Integrin beta-1 (avian) | Developmental Studies Hybridoma Bank CSAT product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Integrin beta-1 (avian)

catalog :

CSAT

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CSAT

reactivity :

chicken

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 3

Reference
Nagy N, Mwizerwa O, Yaniv K, Carmel L, Pieretti Vanmarcke R, Weinstein B, et al. Endothelial cells promote migration and proliferation of enteric neural crest cells via beta1 integrin signaling. Dev Biol. 2009;330:263-72 pubmed publisher
Goel H, Fornaro M, Moro L, Teider N, Rhim J, King M, et al. Selective modulation of type 1 insulin-like growth factor receptor signaling and functions by beta1 integrins. J Cell Biol. 2004;166:407-18 pubmed
Iba K, Albrechtsen R, Gilpin B, Frohlich C, Loechel F, Zolkiewska A, et al. The cysteine-rich domain of human ADAM 12 supports cell adhesion through syndecans and triggers signaling events that lead to beta1 integrin-dependent cell spreading. J Cell Biol. 2000;149:1143-56 pubmed

product information

Internal ID :

4177

Name :

CSAT

Depositor Name :

Horwitz, A.F.

Depositor Institution :

University of Virginia

Depositor Website :

http://www.people.virginia.edu/~afh2n

Date Deposited :

2/3/95

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Integrin beta-1 (avian)

Antigen Species :

Chick

Host Species :

mouse

Isotype :

MIgG2b

Isotype for catalog (legacy) :

IgG2b

Positive Tested Species Reactivity :

Chick,Quail

Species Tested (legacy) :

avian

Initial Publication Pubmed ID :

6183279

Depositor Notes (Special Instructions) :

The cell substrate attachment antibody (CSAT) blocks migrationof neural crest in vivo, prevents neurite outgrowth and blocks myoblast fusion and differentiation in vitro. The antigenic site recognized by CSAT is sensitive to denaturation.

Collections :

CD antigens,Cardiac development,Cell adhesion,Cell signaling,Muscular dystrophy,Neurodevelopment,Skeletal muscle

Search Keywords :

Horwitz, A.F., Integrin beta-1 (avian), Chick, MIgG2b, Chick/Quail, ITGB1, , AB_528142, Monoclonal, Cell adhesion, ELISA/Function Blocking/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

120 kDa and 160 kDa

Gene :

ITGB1

Uniprot ID :

P07228

Antibody Registry ID :

AB_528142

Immunogen :

Membrane blebs from 52-hr cultured myoblasts

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

SP 2/0-Ag14

Epitope Mapped :

Yes

Epitope Location or Sequence :

N-terminus a.a. 1-160

Epitope Map PubMed ID :

7690763

Recommended Applications :

ELISA,Function Blocking,Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

SDS PAGE reveals 2 polypeptides

Immunohistochemistry Pubmed IDs :

19345201

Immunofluorescence Pubmed IDs :

6183279 19345201 18832396 17195179 11803569 14610042 10618164 1371480 8387015 3921554 3653510 7540435

Western Blot Pubmed IDs :

6183279 3131349 10618164 8387015 3921554

Immunoprecipitation Pubmed IDs :

15289498 3131349 7690763 3493247 1706071 3491082

ELISA Pubmed IDs :

6183279

Function Blocking Pubmed IDs :

6183279 26527683 19345201 17239630 14610042 11803569 10831617 10618164 3493247 3115595 8387015 2032798 1725602 3921554 3653510 1706071 3417040 1540708 2539442

FACS Pubmed IDs :

14610042

Additional Information :

In vivo injection of CSAT may be localized in frozen sections (PMID: 2944780). RRID:AB_528142

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 95, 654-666.; Dev. Biol. 150, 82-98.; Cell Motil. Cytoskeleton 45, 27-41.; Pediatr. Res. 64, 326-333.; Dev. Biol. 330(2), 263-272.

more info or order :

Developmental Studies Hybridoma Bank product webpage