product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
creatine kinase
catalog :
CK-5D11
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
CK-5D11
reactivity :
human
application :
immunocytochemistry, immunoprecipitation
citations: 1
Reference
Man N, Cartwright A, Andrews K, Morris G. Treatment of human muscle creatine kinase with glutaraldehyde preferentially increases the immunogenicity of the native conformation and permits production of high-affinity monoclonal antibodies which recognize two distinct surface epitopes. J Immunol Methods. 1989;125:251-9 pubmed
product information
Internal ID :
5097
Name :
CK-5D11
Depositor Name :
Morris, G.E.
Depositor Institution :
Centre for Inherited Neuromuscular Disease
Depositor Website :
http://www.glennmorris.org.uk
Date Deposited :
9/25/08
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
creatine kinase
Antigen Species :
human
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Human
Species Tested (legacy) :
human only (not rat, rabbit, pig, ox or chicken)
Initial Publication Pubmed ID :
2481696
Depositor Notes (Special Instructions) :
This antibody does not Western Blot, itæcaptures native MM-CK from muscle extract. It does not work with rabbits, rats, pigs, or chicken.
Collections :
Enzymes,Human,Muscular dystrophy
Search Keywords :
morris, creatine kinase, CKM, CKMM, monoclonal, IgG1, human, Immunofluorescence,Immunoprecipitation
Antigen Molecular Weight :
43 kDa
Gene :
CKM
Alternate Gene Name(s) :
CKMM
Uniprot ID :
P06732
Antibody Registry ID :
AB_1553661
Clonality :
Monoclonal
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunoprecipitation
Immunoblotting (legacy) :
no
Additional Information :
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. RRID:AB_1553661
Additional Characterization :
http://www.glennmorris.org.uk/datasheets/CK-5D11.doc
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Immunol. Methods 125, 251-259.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US