collagen type II | Developmental Studies Hybridoma Bank CIIC1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

collagen type II

catalog :

CIIC1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CIIC1

reactivity :

human, mouse, rat, chicken, bovine

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 38

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:400; loading ...; fig 5a western blot; mouse; 1:1000; loading ...; fig 5c	Li P, Fleischhauer L, Nicolae C, Prein C, Farkas Z, Saller M, et al. Mice Lacking the Matrilin Family of Extracellular Matrix Proteins Develop Mild Skeletal Abnormalities and Are Susceptible to Age-Associated Osteoarthritis. Int J Mol Sci. 2020;21: pubmed publisher
immunocytochemistry; human; fig 2c	Schminke B, Trautmann S, Mai B, Miosge N, Blaschke S. Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage. Eur J Immunol. 2016;46:440-5 pubmed publisher
immunohistochemistry; mouse	Kan L, Peng C, McGuire T, Kessler J. Glast-expressing progenitor cells contribute to heterotopic ossification. Bone. 2013;53:194-203 pubmed publisher
	Bertram K, Narendran N, Tailor P, Jablonski C, Leonard C, Irvine E, et al. 17-DMAG regulates p21 expression to induce chondrogenesis in vitro and in vivo. Dis Model Mech. 2018;11: pubmed publisher
	Clark A, Worni Schudel I, Korte F, Foster M. A murine Ig light chain transgene reveals IGKV3 gene contributions to anti-collagen types IV and II specificities. Mol Immunol. 2017;91:49-56 pubmed publisher
	Bartolomeo R, Cinque L, De Leonibus C, Forrester A, Salzano A, Monfregola J, et al. mTORC1 hyperactivation arrests bone growth in lysosomal storage disorders by suppressing autophagy. J Clin Invest. 2017;127:3717-3729 pubmed publisher
	Rodriguez Fontan F, Payne K, Chahla J, Mei Dan O, Richards A, Uchida S, et al. Viability and Tissue Quality of Cartilage Flaps From Patients With Femoroacetabular Hip Impingement: A Matched-Control Comparison. Orthop J Sports Med. 2017;5:2325967117723608 pubmed publisher
	Giannoni P, Villa F, Cordazzo C, Zardi L, Fattori P, Quarto R, et al. Rheological properties, biocompatibility and in vivo performance of new hydrogel-based bone fillers. Biomater Sci. 2016;4:1691-1703 pubmed
	Yadav P, Khan M, Prashar P, Duggal S, Rath S, Chattopadhyay N, et al. Characterization of BMP signaling dependent osteogenesis using a BMP depletable avianized bone marrow stromal cell line (TVA-BMSC). Bone. 2016;91:39-52 pubmed publisher
	Leyh M, Seitz A, Dürselen L, Springorum H, Angele P, Ignatius A, et al. Osteoarthritic cartilage explants affect extracellular matrix production and composition in cocultured bone marrow-derived mesenchymal stem cells and articular chondrocytes. Stem Cell Res Ther. 2014;5:77 pubmed publisher
	Rundle C, Chen S, Coen M, Wergedal J, Stiffel V, Lau K. Direct lentiviral-cyclooxygenase 2 application to the tendon-bone interface promotes osteointegration and enhances return of the pull-out tensile strength of the tendon graft in a rat model of biceps tenodesis. PLoS ONE. 2014;9:e98004 pubmed publisher
	Shintani N, Siebenrock K, Hunziker E. TGF-ß1 enhances the BMP-2-induced chondrogenesis of bovine synovial explants and arrests downstream differentiation at an early stage of hypertrophy. PLoS ONE. 2013;8:e53086 pubmed publisher
	Grassel S, Rickert M, Opolka A, Bosserhoff A, Angele P, Grifka J, et al. Coculture between periosteal explants and articular chondrocytes induces expression of TGF-beta1 and collagen I. Rheumatology (Oxford). 2010;49:218-30 pubmed publisher
	Gilbert S, Singhrao S, Khan I, Gonzalez L, Thomson B, Burdon D, et al. Enhanced tissue integration during cartilage repair in vitro can be achieved by inhibiting chondrocyte death at the wound edge. Tissue Eng Part A. 2009;15:1739-49 pubmed publisher
	Nandakumar K, Holmdahl R. Efficient promotion of collagen antibody induced arthritis (CAIA) using four monoclonal antibodies specific for the major epitopes recognized in both collagen induced arthritis and rheumatoid arthritis. J Immunol Methods. 2005;304:126-36 pubmed
	Burkhardt H, Sehnert B, Bockermann R, Engström Å, Kalden J, Holmdahl R. Humoral immune response to citrullinated collagen type II determinants in early rheumatoid arthritis. Eur J Immunol. 2005;35:1643-52 pubmed
	Domm C, Schünke M, Steinhagen J, Freitag S, Kurz B. Influence of various alginate brands on the redifferentiation of dedifferentiated bovine articular chondrocytes in alginate bead culture under high and low oxygen tension. Tissue Eng. 2004;10:1796-805 pubmed
	Davis M, Shubin N, Force A. Pectoral fin and girdle development in the basal actinopterygians Polyodon spathula and Acipenser transmontanus. J Morphol. 2004;262:608-28 pubmed
	Veilleux N, Yannas I, Spector M. Effect of passage number and collagen type on the proliferative, biosynthetic, and contractile activity of adult canine articular chondrocytes in type I and II collagen-glycosaminoglycan matrices in vitro. Tissue Eng. 2004;10:119-27 pubmed
	Gerhart J, Neely C, Stewart B, Perlman J, Beckmann D, Wallon M, et al. Epiblast cells that express MyoD recruit pluripotent cells to the skeletal muscle lineage. J Cell Biol. 2004;164:739-46 pubmed
	Young H, Black A. Adult stem cells. Anat Rec A Discov Mol Cell Evol Biol. 2004;276:75-102 pubmed
	Young H. Existence of reserve quiescent stem cells in adults, from amphibians to humans. Curr Top Microbiol Immunol. 2004;280:71-109 pubmed
	Milz S, Regner F, Putz R, Benjamin M. Expression of a wide range of extracellular matrix molecules in the tendon and trochlea of the human superior oblique muscle. Invest Ophthalmol Vis Sci. 2002;43:1330-4 pubmed
	Milz S, Rufai A, Buettner A, Putz R, Ralphs J, Benjamin M. Three-dimensional reconstructions of the Achilles tendon insertion in man. J Anat. 2002;200:145-52 pubmed
	Young H, Steele T, Bray R, Hudson J, Floyd J, Hawkins K, et al. Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors. Anat Rec. 2001;264:51-62 pubmed
	Young H, Duplaa C, Young T, Floyd J, Reeves M, Davis K, et al. Clonogenic analysis reveals reserve stem cells in postnatal mammals: I. Pluripotent mesenchymal stem cells. Anat Rec. 2001;263:350-60 pubmed
	Milz S, Schlüter T, Putz R, Moriggl B, Ralphs J, Benjamin M. Fibrocartilage in the transverse ligament of the human atlas. Spine (Phila Pa 1976). 2001;26:1765-71 pubmed
	Boszczyk B, Boszczyk A, Putz R, Büttner A, Benjamin M, Milz S. An immunohistochemical study of the dorsal capsule of the lumbar and thoracic facet joints. Spine (Phila Pa 1976). 2001;26:E338-43 pubmed
	Milz S, Valassis G, Büttner A, Maier M, Putz R, Ralphs J, et al. Fibrocartilage in the transverse ligament of the human acetabulum. J Anat. 2001;198:223-8 pubmed
	Milz S, Putz R, Ralphs J, Benjamin M. Fibrocartilage in the extensor tendons of the human metacarpophalangeal joints. Anat Rec. 1999;256:139-45 pubmed
	Milz S, McNeilly C, Putz R, Ralphs J, Benjamin M. Fibrocartilages in the extensor tendons of the interphalangeal joints of human toes. Anat Rec. 1998;252:264-70 pubmed
	Mills D, Daniel J. Development of functional specializations within the maturing rabbit flexor digitorum profundus tendon. Connect Tissue Res. 1993;30:37-57 pubmed
	George Weinstein M, Gerhart J, Foti G, Lash J. Maturation of myogenic and chondrogenic cells in the presomitic mesoderm of the chick embryo. Exp Cell Res. 1994;211:263-74 pubmed
	Aulthouse A, Hitt D. The teratogenic effects of valproic acid in human chondrogenesis in vitro. Teratology. 1994;49:208-17 pubmed
	Aulthouse A, Alroy J. An in vitro model for abnormal skeletal development in the lysosomal storage diseases. Virchows Arch. 1995;426:135-40 pubmed
	Aeschlimann D, Kaupp O, Paulsson M. Transglutaminase-catalyzed matrix cross-linking in differentiating cartilage: identification of osteonectin as a major glutaminyl substrate. J Cell Biol. 1995;129:881-92 pubmed
	Holmdahl R, Rubin K, Klareskog L, Larsson E, Wigzell H. Characterization of the antibody response in mice with type II collagen-induced arthritis, using monoclonal anti-type II collagen antibodies. Arthritis Rheum. 1986;29:400-10 pubmed
	Benjamin M, Ralphs J. Extracellular matrix of connective tissues in the heads of teleosts. J Anat. 1991;179:137-48 pubmed

product information

Internal ID :

4147

Name :

CIIC1

Depositor Name :

Holmdahl, R. / Rubin, K.

Depositor Institution :

University of Uppsala

Date Deposited :

8/31/89

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

collagen type II

Antigen Species :

chicken

Host Species :

mouse

Isotype :

MIgG2a, kappa light chain

Isotype for catalog (legacy) :

IgG2a

Positive Tested Species Reactivity :

Bovine,Chick,Fish,Human,Mouse,Ovine,Rabbit,Rat,Xenopus

Species Tested (legacy) :

human, bovine, chick, rat, mouse, Xenopus, ovine

Initial Publication Pubmed ID :

2421741

Depositor Notes (Special Instructions) :

Stains hyaluronidase-treated fetal and adult mouse and human cartilage in vitro as well as mouse cartilage in vivo. This antibody recognizes native triple-helical type II collagen. It does not bind to denatured type II collagen (PMID: 25005029).

Collections :

Extracellular matrix proteins,Human,Stem cell,Xenopus

Search Keywords :

Holmdahl, R. /Rubin, K., collagen type II, chicken, MIgG2a, kappa light chain, human/bovine/chick/rat/mouse/Xenopus/ovine/fish, COL2A1, , AB_528164, monoclonal, Extracellular matrix proteins/Human/Stem cell/Xenopus, Immunohistochemistry/Immunofluorescence/Western Blot/ELISA

Antigen Molecular Weight :

Predicted: 125 kDa

Gene :

COL2A1

Uniprot ID :

H9L2B7

Antibody Registry ID :

AB_528164

Synonyms (Alternate Clone Names) :

CIICI, C1

Immunogen :

native chick type I1 collagen

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

X63.Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

The ARGLT epitope is located at aa 359-363 of the human collagen II triple helical region

Epitope Map PubMed ID :

16125192

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

9776080 10486511 11500811 11505371 11474365 11493848 11273046 11895112 11980843 14594208 14699636 16125192 15684688 15376275 27709133 28868322

Immunofluorescence Pubmed IDs :

8143772 8059428 7757283 19952089 19119922 1817131

Western Blot Pubmed IDs :

7730416 15009937 15832289 15684688

ELISA Pubmed IDs :

15832289 28886586

FFPE Pubmed IDs :

27709133

Pubmed IDs :

8149743

Additional Information :

The CIIC1 antibody induces sub clinical arthritis if injected at low (0.5 mg) doses and clinical arthritis in mice if injected at higher doses and in combination with other antibodies (see [PMID: 16125192]).

DSHB Growth Medium :

Iscove's

References (legacy) :

Arthritis Rheum. 29, 400-410.; Corr. Tissue Res. 30, 37-57.; Exp. Cell Res. 211, 263-274.; J. Cell Biol. 129, 881-892.; Teratology 49, 208-217.; Virchows Archiv 426, 135-140.; Anat. Rec. 252, 264-270.; Anat. Rec. 256, 139-145.; Anat. Rec. 263, 350-360.; Anat. Rec. 264, 51-62.; Spine 26(15), E338-E343.; Spine 26(16), 1765-1771.; J. Anat. 198, 223-228.; J. Anat. 200, 145-152.; Invest. Ophthalmol. Vis. Sci. 43(5), 1330-1334.; Curr. Top. Microbiol. Immunol. 280, 71-109.; Anat. Rec. Part A 276A, 75-102.

more info or order :

Developmental Studies Hybridoma Bank product webpage