tropomyosin | Developmental Studies Hybridoma Bank CH291 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

tropomyosin

catalog :

CH291

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CH291

reactivity :

human, chicken

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 7

Reference
Marston S, Copeland O, Messer A, Macnamara E, Nowak K, Zampronio C, et al. Tropomyosin isoform expression and phosphorylation in the human heart in health and disease. J Muscle Res Cell Motil. 2013;34:189-97 pubmed publisher
L Ecuyer T, Noller J, Fulton A. Assembly of tropomyosin isoforms into the cytoskeleton of avian muscle cells. Pediatr Res. 1998;43:813-22 pubmed
Sieber Blum M, Ito K, Richardson M, Langtimm C, Duff R. Distribution of pluripotent neural crest cells in the embryo and the role of brain-derived neurotrophic factor in the commitment to the primary sensory neuron lineage. J Neurobiol. 1993;24:173-84 pubmed
Sieber Blum M, Ito K. In vitro clonal analysis of cardiac outflow tract mesenchyme. Ann N Y Acad Sci. 1995;752:92-100 pubmed
Lin J, Chou C, Lin J. Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application. Hybridoma. 1985;4:223-42 pubmed
Hegmann T, Lin J, Lin J. Probing the role of nonmuscle tropomyosin isoforms in intracellular granule movement by microinjection of monoclonal antibodies. J Cell Biol. 1989;109:1141-52 pubmed
Lourim D, Lin J. Expression of nuclear lamin A and muscle-specific proteins in differentiating muscle cells in ovo and in vitro. J Cell Biol. 1989;109:495-504 pubmed

product information

Internal ID :

7103

Name :

CH291

Depositor Name :

Lin, J.J.-C.

Depositor Institution :

University of Iowa

Date Deposited :

3/22/11

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

tropomyosin

Antigen Species :

Chicken

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Broad species,Chicken,Human

Species Tested (legacy) :

Broad species-specific

Initial Publication Pubmed ID :

3899907

Collections :

Cardiac development,Cytoskeleton,Human,Skeletal muscle

Search Keywords :

Lin, J.J.-C., tropomyosin, Chicken, MIgG1, broad species/Human/chicken, TPM1, , AB_10807224, monoclonal, Cardiac development/Human/Skeletal muscle/Cytoskeletal elements, Immunoprecipitation/Immunofluorescence/Western Blot/Function Blocking

Antigen Molecular Weight :

Apparent: 39 kDa

Gene :

TPM1

Uniprot ID :

P04268

Antibody Registry ID :

AB_10807224

Immunogen :

Purified chicken cardiac muscle tropomyosin

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS1

Epitope Mapped :

Recommended Applications :

Function Blocking,Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

8445386 7755300 2668298

Western Blot Pubmed IDs :

3899907 2670955 2668298 23712688 9621993

Immunoprecipitation Pubmed IDs :

2670955

Function Blocking Pubmed IDs :

3899907

Additional Information :

CH291 antibody reacts with chicken cardiac alpha-tropomyosin and skeletal alpha-tropomyosin but not with other isoforms of tropomyosin, including skeletal beta-tropomyosin. CH291 interferes with the binding of tropomyosin to actin. CH291 is specific to ?-tropomyosin in the presence of ?-tropomyosin [PMID: 23712688].

DSHB Growth Medium :

RPMI

References (legacy) :

Hybridoma 4, 223-242.

more info or order :

Developmental Studies Hybridoma Bank product webpage