tropomyosin, striated muscle | Developmental Studies Hybridoma Bank CH1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

tropomyosin, striated muscle

catalog :

CH1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CH1

reactivity :

jerboas, human, mouse, bantam, leopard danio, Xenopus laevis

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 36

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; jerboas; 1:10; loading ...; fig 5a	Tran M, Tsutsumi R, Erberich J, Chen K, Flores M, Cooper K. Evolutionary loss of foot muscle during development with characteristics of atrophy and no evidence of cell death. elife. 2019;8: pubmed publisher
immunohistochemistry - frozen section; leopard danio; 1:100; fig s2	Sallin P, Jazwinska A. Acute stress is detrimental to heart regeneration in zebrafish. Open Biol. 2016;6: pubmed publisher
immunohistochemistry; Xenopus laevis; 1:25; fig 5	Tandon P, Wilczewski C, Williams C, Conlon F. The Lhx9-integrin pathway is essential for positioning of the proepicardial organ. Development. 2016;143:831-40 pubmed publisher
immunocytochemistry; human; fig s5	Wang Y, Li Z, Zhang P, Poon E, Kong C, Boheler K, et al. Nitric Oxide-cGMP-PKG Pathway Acts on Orai1 to Inhibit the Hypertrophy of Human Embryonic Stem Cell-Derived Cardiomyocytes. Stem Cells. 2015;33:2973-84 pubmed publisher
immunohistochemistry; Xenopus laevis; 1:10; fig 1	Amin N, Gibbs D, Conlon F. Differential regulation of CASZ1 protein expression during cardiac and skeletal muscle development. Dev Dyn. 2014;243:948-56 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100	Delgado Olguin P, Huang Y, Li X, Christodoulou D, Seidman C, Seidman J, et al. Epigenetic repression of cardiac progenitor gene expression by Ezh2 is required for postnatal cardiac homeostasis. Nat Genet. 2012;44:343-7 pubmed publisher
	Crowell K, Laufenberg L, Lang C. Chronic Alcohol Consumption, but not Acute Intoxication, Decreases In Vitro Skeletal Muscle Contractile Function. Alcohol Clin Exp Res. 2019;43:2090-2099 pubmed publisher
	Kitakaze T, Sakamoto T, Kitano T, Inoue N, Sugihara F, Harada N, et al. The collagen derived dipeptide hydroxyprolyl-glycine promotes C2C12 myoblast differentiation and myotube hypertrophy. Biochem Biophys Res Commun. 2016;478:1292-7 pubmed publisher
	Amarasinghe C, Hossain M, Jin J. Functional Basis of Three New Recessive Mutations of Slow Skeletal Muscle Troponin T Found in Non-Amish TNNT1 Nemaline Myopathies. Biochemistry. 2016;55:4560-7 pubmed publisher
	England J, Pang K, Parnall M, Haig M, Loughna S. Cardiac troponin T is necessary for normal development in the embryonic chick heart. J Anat. 2016;229:436-49 pubmed publisher
	Suzuki D, Fukumoto Y, Yoshimura M, Yamazaki Y, Kosaka J, Kuratani S, et al. Comparative morphology and development of extra-ocular muscles in the lamprey and gnathostomes reveal the ancestral state and developmental patterns of the vertebrate head. Zoological Lett. 2016;2:10 pubmed publisher
	Feng H, Chen X, Malek M, Jin J. Slow recovery of the impaired fatigue resistance in postunloading mouse soleus muscle corresponding to decreased mitochondrial function and a compensatory increase in type I slow fibers. Am J Physiol Cell Physiol. 2016;310:C27-40 pubmed publisher
	Wu Q, Zhang J, Koh W, Yu Q, Zhu X, Amsterdam A, et al. Talin1 is required for cardiac Z-disk stabilization and endothelial integrity in zebrafish. FASEB J. 2015;29:4989-5005 pubmed publisher
	Dube S, Yalamanchili S, Lachant J, Abbott L, Benz P, Mitschow C, et al. Expression of Tropomyosin 1 Gene Isoforms in Human Breast Cancer Cell Lines. Int J Breast Cancer. 2015;2015:859427 pubmed publisher
	Pittner S, Monticelli F, Pfisterer A, Zissler A, Sänger A, Stoiber W, et al. Postmortem degradation of skeletal muscle proteins: a novel approach to determine the time since death. Int J Legal Med. 2016;130:421-31 pubmed publisher
	Amarasinghe C, Jin J. N-Terminal Hypervariable Region of Muscle Type Isoforms of Troponin T Differentially Modulates the Affinity of Tropomyosin-Binding Site 1. Biochemistry. 2015;54:3822-30 pubmed publisher
	Zajdel R, McLean M, Dube S, Dube D. Expression of tropomyosin in relation to myofibrillogenesis in axolotl hearts. Regen Med Res. 2013;1:8 pubmed publisher
	Wang X, Wang X, Yuan W, Chai R, Liu D. Egfl6 is involved in zebrafish notochord development. Fish Physiol Biochem. 2015;41:961-9 pubmed publisher
	Nan C, Dube S, Matoq A, Mikesell L, Abbott L, Alshiekh Nasany R, et al. Expression of sarcomeric tropomyosin in striated muscles in axolotl treated with shz-1, a small cardiogenic molecule. Cardiovasc Toxicol. 2015;15:29-40 pubmed publisher
	Marston S, Copeland O, Messer A, Macnamara E, Nowak K, Zampronio C, et al. Tropomyosin isoform expression and phosphorylation in the human heart in health and disease. J Muscle Res Cell Motil. 2013;34:189-97 pubmed publisher
	Schevzov G, Whittaker S, Fath T, Lin J, Gunning P. Tropomyosin isoforms and reagents. Bioarchitecture. 2011;1:135-164 pubmed
	Takeuchi J, Lou X, Alexander J, Sugizaki H, Delgado Olguin P, Holloway A, et al. Chromatin remodelling complex dosage modulates transcription factor function in heart development. Nat Commun. 2011;2:187 pubmed publisher
	Murphy R. Enhanced technique to measure proteins in single segments of human skeletal muscle fibers: fiber-type dependence of AMPK-alpha1 and -beta1. J Appl Physiol (1985). 2011;110:820-5 pubmed publisher
	Biesiadecki B, Chong S, Nosek T, Jin J. Troponin T core structure and the regulatory NH2-terminal variable region. Biochemistry. 2007;46:1368-79 pubmed
	Zhang Z, Biesiadecki B, Jin J. Selective deletion of the NH2-terminal variable region of cardiac troponin T in ischemia reperfusion by myofibril-associated mu-calpain cleavage. Biochemistry. 2006;45:11681-94 pubmed
	Wang Y, Qian L, Yu Z, Jiang Q, Dong Y, Liu X, et al. Requirements of myocyte-specific enhancer factor 2A in zebrafish cardiac contractility. FEBS Lett. 2005;579:4843-50 pubmed
	Wang Y, Zhong T, Qian L, Dong Y, Jiang Q, Tan L, et al. Wortmannin induces zebrafish cardia bifida through a mechanism independent of phosphoinositide 3-kinase and myosin light chain kinase. Biochem Biophys Res Commun. 2005;331:303-8 pubmed
	Denz C, Narshi A, Zajdel R, Dube D. Expression of a novel cardiac-specific tropomyosin isoform in humans. Biochem Biophys Res Commun. 2004;320:1291-7 pubmed
	Kolker S, Tajchman U, Weeks D. Confocal imaging of early heart development in Xenopus laevis. Dev Biol. 2000;218:64-73 pubmed
	Zajdel R, McLean M, Lemanski S, Muthuchamy M, Wieczorek D, Lemanski L, et al. Ectopic expression of tropomyosin promotes myofibrillogenesis in mutant axolotl hearts. Dev Dyn. 1998;213:412-20 pubmed
	Begum S, Komiyama M, Toyota N, Obinata T, Maruyama K, Shimada Y. Differentiation of muscle-specific proteins in chicken somites as studied by immunofluorescence microscopy. Cell Tissue Res. 1998;293:305-11 pubmed
	L Ecuyer T, Noller J, Fulton A. Assembly of tropomyosin isoforms into the cytoskeleton of avian muscle cells. Pediatr Res. 1998;43:813-22 pubmed
	Muthuchamy M, Grupp I, Grupp G, O Toole B, Kier A, Boivin G, et al. Molecular and physiological effects of overexpressing striated muscle beta-tropomyosin in the adult murine heart. J Biol Chem. 1995;270:30593-603 pubmed
	Lin J, Chou C, Lin J. Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application. Hybridoma. 1985;4:223-42 pubmed
	Lourim D, Lin J. Expression of nuclear lamin A and muscle-specific proteins in differentiating muscle cells in ovo and in vitro. J Cell Biol. 1989;109:495-504 pubmed
	Maier A, Zak R. Arrangement of cytoskeletal filaments at the equator of chicken intrafusal muscle fibers. Histochemistry. 1990;93:423-8 pubmed

product information

Internal ID :

2988

Name :

CH1

Depositor Name :

Lin, J.J.-C.

Depositor Institution :

University of Iowa

Date Deposited :

9/8/88

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

tropomyosin, striated muscle

Antigen Species :

Chicken

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Axolotl,Broad species,Chicken,Fish,Human,Mouse,Rat,Xenopus

Species Tested (legacy) :

chicken, rat, mouse, Xenopus

Initial Publication Pubmed ID :

3899907

Collections :

Cardiac development,Cytoskeleton,Human,Muscular dystrophy,Skeletal muscle,Xenopus

Search Keywords :

Lin, J.J.-C., tropomyosin, striated muscle, Chicken, MIgG1, chicken/rat/mouse/Xenopus/Human/Axolotl/fish/broad species, TPM1, , AB_2205770, monoclonal, Cardiac development/Muscular dystrophy/Skeletal muscle/Xenopus/Cytoskeletal elements/Human, Immunofluorescence/Western Blot/ELISA/FFPE/Function Blocking/Immunoprecipitation

Antigen Molecular Weight :

Apparent: 39 kDa (or smaller depending on the isoform)

Gene :

TPM1

Uniprot ID :

P04268

Antibody Registry ID :

AB_2205770

Immunogen :

Purified chicken cardiac muscle tropomyosin

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS1

Epitope Mapped :

Yes

Epitope Location or Sequence :

CH1 has been reported to bind to the peptide region encoded by exon 9a of TPM genes [PMID: 24958154]; this exon is well conserved in all vertebrates. But, the binding of CH1 has also been described to be limited to alpha9a [PMID: 22069507].

Epitope Map PubMed ID :

24958154 22069507

Recommended Applications :

ELISA,FFPE,Function Blocking,Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

15249230 9853962 26310270 25952972 27194630 27081572 25984327 24633745 2323957 9662653 10644411 16107252 15845393

Western Blot Pubmed IDs :

3899907 21088205 26171250 24958154 26041514 27553280 26447205 25984327 8530495 2668298 23712688 31411733

Immunoprecipitation Pubmed IDs :

9621993

ELISA Pubmed IDs :

26024675 16981728 27429059 17260966

Function Blocking Pubmed IDs :

3899907

Additional Information :

CH1 reacts strongly with striated muscle forms of tropomyosin, including cardiac alpha-tropomyosin and skeletal alpha- and beta-tropomyosins, but not with tropomyosin isoforms from gizzard (smooth muscle) or chicken embryo fibroblasts. CH1 interferes with the binding of tropomyosin to actin. CH1 recognizes sarcomeric Tm soforms alpha-fast, beta, and alpha-slow with poor affinity for alpha-slow.

DSHB Growth Medium :

RPMI

References (legacy) :

Hybridoma 4, 223-242.; Histochem. 93, 423-428.; J. Tiss. Cult. Meth. 14, 31-36.; Cell Tissue Res. 293, 305-311.; Dev. Biol. 218, 64-73.; FEBS Lett. 579, 4843-4850.; Biochem. Biophys. Res. Commun. 331, 303-308.

more info or order :

Developmental Studies Hybridoma Bank product webpage