tropomyosin, TPM1 | Developmental Studies Hybridoma Bank CGbeta6 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

tropomyosin, TPM1

catalog :

CGbeta6

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CGbeta6

reactivity :

human, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 19

Reference
Glass J, Phillips P, Gunning P, Stehn J. Hypoxia alters the recruitment of tropomyosins into the actin stress fibres of neuroblastoma cells. BMC Cancer. 2015;15:712 pubmed publisher
Prunotto M, Bruschi M, Gunning P, Gabbiani G, Weibel F, Ghiggeri G, et al. Stable incorporation of Î±-smooth muscle actin into stress fibers is dependent on specific tropomyosin isoforms. Cytoskeleton (Hoboken). 2015;72:257-67 pubmed publisher
Liu R, Feng H, Jin J. Physiological contractility of cardiomyocytes in the wall of mouse and rat azygos vein. Am J Physiol Cell Physiol. 2014;306:C697-704 pubmed publisher
Schevzov G, Whittaker S, Fath T, Lin J, Gunning P. Tropomyosin isoforms and reagents. Bioarchitecture. 2011;1:135-164 pubmed
Gallant C, Appel S, Graceffa P, Leavis P, Lin J, Gunning P, et al. Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells. Am J Physiol Cell Physiol. 2011;300:C1356-65 pubmed publisher
Lin J, Eppinga R, Warren K, McCrae K. Human tropomyosin isoforms in the regulation of cytoskeleton functions. Adv Exp Med Biol. 2008;644:201-22 pubmed
Das K, Bajpai M. Tropomyosins in human diseases: ulcerative colitis. Adv Exp Med Biol. 2008;644:158-67 pubmed
Mirza Z, Sastri B, Lin J, Amenta P, Das K. Autoimmunity against human tropomyosin isoforms in ulcerative colitis: localization of specific human tropomyosin isoforms in the intestine and extraintestinal organs. Inflamm Bowel Dis. 2006;12:1036-43 pubmed
Eppinga R, Li Y, Lin J, Lin J. Tropomyosin and caldesmon regulate cytokinesis speed and membrane stability during cell division. Arch Biochem Biophys. 2006;456:161-74 pubmed
O Hara S, Lin J. Accumulation of tropomyosin isoform 5 at the infection sites of host cells during Cryptosporidium invasion. Parasitol Res. 2006;99:45-54 pubmed
DesMarais V, Ichetovkin I, Condeelis J, Hitchcock Degregori S. Spatial regulation of actin dynamics: a tropomyosin-free, actin-rich compartment at the leading edge. J Cell Sci. 2002;115:4649-60 pubmed
Geng X, Biancone L, Dai H, Lin J, Yoshizaki N, Dasgupta A, et al. Tropomyosin isoforms in intestinal mucosa: production of autoantibodies to tropomyosin isoforms in ulcerative colitis. Gastroenterology. 1998;114:912-22 pubmed
Pelham R, Lin J, Wang Y. A high molecular mass non-muscle tropomyosin isoform stimulates retrograde organelle transport. J Cell Sci. 1996;109 ( Pt 5):981-9 pubmed
Novy R, Liu L, Lin C, Helfman D, Lin J. Expression of smooth muscle and nonmuscle tropomyosins in Escherichia coli and characterization of bacterially produced tropomyosins. Biochim Biophys Acta. 1993;1162:255-65 pubmed
Warren K, Lin J, McDermott J, Lin J. Forced expression of chimeric human fibroblast tropomyosin mutants affects cytokinesis. J Cell Biol. 1995;129:697-708 pubmed
Lin J, Chou C, Lin J. Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application. Hybridoma. 1985;4:223-42 pubmed
Lin J, Hegmann T, Lin J. Differential localization of tropomyosin isoforms in cultured nonmuscle cells. J Cell Biol. 1988;107:563-72 pubmed
Hegmann T, Lin J, Lin J. Probing the role of nonmuscle tropomyosin isoforms in intracellular granule movement by microinjection of monoclonal antibodies. J Cell Biol. 1989;109:1141-52 pubmed
Hegmann T, Lin J, Lin J. Motility-dependence of the heterogenous staining of culture cells by a monoclonal anti-tropomyosin antibody. J Cell Biol. 1988;106:385-93 pubmed

product information

Internal ID :

6132

Name :

CGbeta6

Depositor Name :

Lin, J.J.-C.

Depositor Institution :

University of Iowa

Date Deposited :

7/17/09

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

tropomyosin, TPM1

Antigen Species :

Chicken

Host Species :

mouse

Isotype :

MIgM

Isotype for catalog (legacy) :

IgM

Positive Tested Species Reactivity :

Chicken,Human,Rodent

Species Tested (legacy) :

human tropomyosin isoforms 2 and 3 (hTM and hTM3), rodent and chicken tropomyosins

Initial Publication Pubmed ID :

3899907

Collections :

Cytoskeleton,Human,Skeletal muscle

Search Keywords :

Lin, J.J.-C., tropomyosin, TPM1, Chicken, MIgM, human/rodent/chicken, TPM1, , AB_10573118, monoclonal, Cytoskeletal elements/skeletal muscle/Human, Immunohistochemistry/Immunofluorescence/Immunoprecipitation/Western Blot

Antigen Molecular Weight :

Apparent: 34kDa

Gene :

TPM1

Uniprot ID :

P04268

Antibody Registry ID :

AB_10573118

Immunogen :

purified chicken gizzard tropomyosin

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS1

Epitope Mapped :

Yes

Epitope Location or Sequence :

C-terminal end of Human Tm2 and Tm3 (TPM1)

Epitope Map PubMed ID :

22069507

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

17075344 19209821

Immunofluorescence Pubmed IDs :

7730405 22069507 26147585 26475688 3047141 19209824 16854366 8743945

Western Blot Pubmed IDs :

3899907 2670955 2448313 7730405 8457589 9558279 26147585 26475688 24477237 17075344 3047141 16479376 12415009 21289288

Immunoprecipitation Pubmed IDs :

2670955

Additional Information :

CGbeta6 recognizes the slow-migrating component of gizzard tropomyosin and cross-reacts with chicken embryo fibroblasts tropomyosin, particularly isoforms a, b, 1, and 2, but does not recognize tropomyosin isoforms from striated muscle. Recognizes Human and rat Tm2 and Tm3 (encoded by TPM1) and rat Tm5a (TPM1) [PMID: 22069507].

DSHB Growth Medium :

Iscove's

References (legacy) :

Hybridoma 4, 223-242.; J. Cell Biol. 106, 385-393.; J. Cell Biol. 107, 563-572.; J. Cell Biol. 109, 1141-1152.; Cell Motil. Cytoskel. 26, 248-261.; J. Cell Biol. 129, 697-708.; Gasteroenterology 114, 912-922.; Parasitol. Res. 99, 45-54.; Inflamm. Bowel Dis. 12, 1036-1043.; Adv. Exp. Med. Biol. 644, 201.222.

more info or order :

Developmental Studies Hybridoma Bank product webpage