product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
tropomyosin, TPM3
catalog :
CG3
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
CG3
reactivity :
chicken
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS
more info or order :
citations: 27
| Reference |
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Schevzov G, Whittaker S, Fath T, Lin J, Gunning P. Tropomyosin isoforms and reagents. Bioarchitecture. 2011;1:135-164 pubmed
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Das K, Bajpai M. Tropomyosins in human diseases: ulcerative colitis. Adv Exp Med Biol. 2008;644:158-67 pubmed
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Mirza Z, Sastri B, Lin J, Amenta P, Das K. Autoimmunity against human tropomyosin isoforms in ulcerative colitis: localization of specific human tropomyosin isoforms in the intestine and extraintestinal organs. Inflamm Bowel Dis. 2006;12:1036-43 pubmed
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O Hara S, Lin J. Accumulation of tropomyosin isoform 5 at the infection sites of host cells during Cryptosporidium invasion. Parasitol Res. 2006;99:45-54 pubmed
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Schevzov G, Bryce N, Almonte Baldonado R, Joya J, Lin J, Hardeman E, et al. Specific features of neuronal size and shape are regulated by tropomyosin isoforms. Mol Biol Cell. 2005;16:3425-37 pubmed
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Schevzov G, Vrhovski B, Bryce N, Elmir S, Qiu M, O Neill G, et al. Tissue-specific tropomyosin isoform composition. J Histochem Cytochem. 2005;53:557-70 pubmed
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Kee A, Schevzov G, Nair Shalliker V, Robinson C, Vrhovski B, Ghoddusi M, et al. Sorting of a nonmuscle tropomyosin to a novel cytoskeletal compartment in skeletal muscle results in muscular dystrophy. J Cell Biol. 2004;166:685-96 pubmed
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Pawlak G, McGarvey T, Nguyen T, Tomaszewski J, Puthiyaveettil R, Malkowicz S, et al. Alterations in tropomyosin isoform expression in human transitional cell carcinoma of the urinary bladder. Int J Cancer. 2004;110:368-73 pubmed
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Ashworth S, Wean S, Campos S, Temm Grove C, Southgate E, Vrhovski B, et al. Renal ischemia induces tropomyosin dissociation-destabilizing microvilli microfilaments. Am J Physiol Renal Physiol. 2004;286:F988-96 pubmed
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DesMarais V, Ichetovkin I, Condeelis J, Hitchcock Degregori S. Spatial regulation of actin dynamics: a tropomyosin-free, actin-rich compartment at the leading edge. J Cell Sci. 2002;115:4649-60 pubmed
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Percival J, Thomas G, Cock T, Gardiner E, Jeffrey P, Lin J, et al. Sorting of tropomyosin isoforms in synchronised NIH 3T3 fibroblasts: evidence for distinct microfilament populations. Cell Motil Cytoskeleton. 2000;47:189-208 pubmed
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Vera C, Sood A, Gao K, Yee L, Lin J, Sung L. Tropomodulin-binding site mapped to residues 7-14 at the N-terminal heptad repeats of tropomyosin isoform 5. Arch Biochem Biophys. 2000;378:16-24 pubmed
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Sung L, Gao K, Yee L, Temm Grove C, Helfman D, Lin J, et al. Tropomyosin isoform 5b is expressed in human erythrocytes: implications of tropomodulin-TM5 or tropomodulin-TM5b complexes in the protofilament and hexagonal organization of membrane skeletons. Blood. 2000;95:1473-80 pubmed
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Kesari K, Yoshizaki N, Geng X, Lin J, Das K. Externalization of tropomyosin isoform 5 in colon epithelial cells. Clin Exp Immunol. 1999;118:219-27 pubmed
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Geng X, Biancone L, Dai H, Lin J, Yoshizaki N, Dasgupta A, et al. Tropomyosin isoforms in intestinal mucosa: production of autoantibodies to tropomyosin isoforms in ulcerative colitis. Gastroenterology. 1998;114:912-22 pubmed
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Novy R, Sellers J, Liu L, Lin J. In vitro functional characterization of bacterially expressed human fibroblast tropomyosin isoforms and their chimeric mutants. Cell Motil Cytoskeleton. 1993;26:248-61 pubmed
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Warren K, Lin J, Wamboldt D, Lin J. Overexpression of human fibroblast caldesmon fragment containing actin-, Ca++/calmodulin-, and tropomyosin-binding domains stabilizes endogenous tropomyosin and microfilaments. J Cell Biol. 1994;125:359-68 pubmed
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Warren K, Lin J, McDermott J, Lin J. Forced expression of chimeric human fibroblast tropomyosin mutants affects cytokinesis. J Cell Biol. 1995;129:697-708 pubmed
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Lin J, Chou C, Lin J. Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application. Hybridoma. 1985;4:223-42 pubmed
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Lin J, Hegmann T, Lin J. Differential localization of tropomyosin isoforms in cultured nonmuscle cells. J Cell Biol. 1988;107:563-72 pubmed
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Hegmann T, Lin J, Lin J. Probing the role of nonmuscle tropomyosin isoforms in intracellular granule movement by microinjection of monoclonal antibodies. J Cell Biol. 1989;109:1141-52 pubmed
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Hegmann T, Lin J, Lin J. Motility-dependence of the heterogenous staining of culture cells by a monoclonal anti-tropomyosin antibody. J Cell Biol. 1988;106:385-93 pubmed
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product information
Internal ID :
6127
Name :
CG3
Depositor Name :
Lin, J.J.-C.
Depositor Institution :
University of Iowa
Date Deposited :
7/17/09
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
tropomyosin, TPM3
Antigen Species :
Chicken
Host Species :
mouse
Isotype :
MIgM
Isotype for catalog (legacy) :
IgM
Positive Tested Species Reactivity :
Chicken,Human,Rodent
Species Tested (legacy) :
human tropomyosin 5 (aa#29-44), rodent TM5 and chicken tropomyosins
Initial Publication Pubmed ID :
3899907
Collections :
Cytoskeleton,Human,Skeletal muscle
Search Keywords :
Lin, J.J.-C., tropomyosin, TPM3, Chicken, MIgM, human/rodent/chicken, TPM3, , AB_10572305, monoclonal, Cytoskeletal elements/skeletal muscle/Human, Immunohistochemistry/Immunofluorescence/Immunoprecipitation/Western Blot/FACS
Antigen Molecular Weight :
Apparent: 32 kDa
Gene :
TPM3
Antibody Registry ID :
AB_10572305
Immunogen :
purified chicken gizzard tropomyosin
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
NS1
Epitope Mapped :
Yes
Epitope Location or Sequence :
In Human, aa 29-44 encoded by exon 1b of TPM3 (AERLQREVEGERRAREQ)
Epitope Map PubMed ID :
22069507
Recommended Applications :
FACS,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
17075344 15337777 16479376 19209821
Immunofluorescence Pubmed IDs :
3899907 7730405 22069507 26475688 27575021 25408381 3047141 8163552 11056521 15337777 16479376 12415009
Western Blot Pubmed IDs :
3899907 2670955 23712688 2448313 22069507 9558279 17075344 3047141 8163552 10540182 11056521 10666227 10871039 15337777 15872049 16479376 18987115 10871039 10666227 12415009 15095301 15075195
Immunoprecipitation Pubmed IDs :
2670955
FACS Pubmed IDs :
10540182
Pubmed IDs :
8293480
Additional Information :
CG3 recognizes all chicken tropomyosin isoforms [PMID: 3899907]. CG3 is specific to all cytoskeletal products from the TPM3 gene [PMID: 22069507], both human and mouse. Can also detect the striated muscle isoform encoded by this gene by cross reacting with theTPM3 exons 1a and 2b products.
DSHB Growth Medium :
Iscove's
References (legacy) :
Hybridoma 4, 223-242.; J. Cell Biol. 106, 385-393.; J. Cell Biol. 109, 1141-1152.; Cell Motil. Cytoskel. 26, 248-261.; J. Cell Biol. 125, 359-368.; J. Cell Biol. 129, 697-708.; Gastroenterology 114, 912-922.; Clin. Exp. Immunol. 118, 219-227.; Cell Motil. Cytoskel. 47, 189-208.; Blood 95, 1473-1480.; Arch. Biochem. Biophys. 378, 16-24.; J. Cell Biol. 166, 685-696. ; Mol Biol. Cell 16, 3425-3437.; Parasitol. Res. 99, 45-54.; Inflamm. Bowel Dis. 12, 1036-1043.; Am. J. Physiol. Renal Physiol. 296, F186-F193.
more info or order :
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Developmental Studies Hybridoma Bank
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