tropomyosin, TPM2 | Developmental Studies Hybridoma Bank CG1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

tropomyosin, TPM2

catalog :

CG1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CG1

reactivity :

chicken

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 13

Reference
Brayford S, Bryce N, Schevzov G, Haynes E, Bear J, Hardeman E, et al. Tropomyosin Promotes Lamellipodial Persistence by Collaborating with Arp2/3 at the Leading Edge. Curr Biol. 2016;26:1312-8 pubmed publisher
Prunotto M, Bruschi M, Gunning P, Gabbiani G, Weibel F, Ghiggeri G, et al. Stable incorporation of Î±-smooth muscle actin into stress fibers is dependent on specific tropomyosin isoforms. Cytoskeleton (Hoboken). 2015;72:257-67 pubmed publisher
Liu R, Feng H, Jin J. Physiological contractility of cardiomyocytes in the wall of mouse and rat azygos vein. Am J Physiol Cell Physiol. 2014;306:C697-704 pubmed publisher
Schevzov G, Whittaker S, Fath T, Lin J, Gunning P. Tropomyosin isoforms and reagents. Bioarchitecture. 2011;1:135-164 pubmed
Gallant C, Appel S, Graceffa P, Leavis P, Lin J, Gunning P, et al. Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells. Am J Physiol Cell Physiol. 2011;300:C1356-65 pubmed publisher
Mirza Z, Sastri B, Lin J, Amenta P, Das K. Autoimmunity against human tropomyosin isoforms in ulcerative colitis: localization of specific human tropomyosin isoforms in the intestine and extraintestinal organs. Inflamm Bowel Dis. 2006;12:1036-43 pubmed
O Hara S, Lin J. Accumulation of tropomyosin isoform 5 at the infection sites of host cells during Cryptosporidium invasion. Parasitol Res. 2006;99:45-54 pubmed
Kesari K, Yoshizaki N, Geng X, Lin J, Das K. Externalization of tropomyosin isoform 5 in colon epithelial cells. Clin Exp Immunol. 1999;118:219-27 pubmed
Geng X, Biancone L, Dai H, Lin J, Yoshizaki N, Dasgupta A, et al. Tropomyosin isoforms in intestinal mucosa: production of autoantibodies to tropomyosin isoforms in ulcerative colitis. Gastroenterology. 1998;114:912-22 pubmed
Novy R, Liu L, Lin C, Helfman D, Lin J. Expression of smooth muscle and nonmuscle tropomyosins in Escherichia coli and characterization of bacterially produced tropomyosins. Biochim Biophys Acta. 1993;1162:255-65 pubmed
Lin J, Chou C, Lin J. Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application. Hybridoma. 1985;4:223-42 pubmed
Hegmann T, Lin J, Lin J. Probing the role of nonmuscle tropomyosin isoforms in intracellular granule movement by microinjection of monoclonal antibodies. J Cell Biol. 1989;109:1141-52 pubmed
Hegmann T, Lin J, Lin J. Motility-dependence of the heterogenous staining of culture cells by a monoclonal anti-tropomyosin antibody. J Cell Biol. 1988;106:385-93 pubmed

product information

Internal ID :

6122

Name :

CG1

Depositor Name :

Lin, J.J.-C.

Depositor Institution :

University of Iowa

Date Deposited :

7/17/09

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

tropomyosin, TPM2

Antigen Species :

Chicken

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Chicken,Ferret,Human,Rodent

Species Tested (legacy) :

human tropomyosin 1, ferret, rodent and chicken

Initial Publication Pubmed ID :

3899907

Collections :

Cytoskeleton,Human,Skeletal muscle

Search Keywords :

Lin, J.J.-C., tropomyosin, TPM2, Chicken, MIgG1, human/ferret/rodent/chicken, TPM2, , AB_10573117, monoclonal, Cytoskeletal elements/skeletal muscle/Human, Immunohistochemistry/Immunofluorescence/Immunoprecipitation/Western Blot/Function Blocking/FACS

Antigen Molecular Weight :

Apparent: 36 kDa (Tm1), 30 kDa (Tm4)

Gene :

TPM2

Uniprot ID :

P19352

Antibody Registry ID :

AB_10573117

Immunogen :

purified chicken gizzard tropomyosin

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS1

Epitope Mapped :

Recommended Applications :

FACS,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

17075344

Immunofluorescence Pubmed IDs :

3899907 2448313 22069507 27112294 26147585

Western Blot Pubmed IDs :

3899907 2670955 2448313 8457589 9558279 26147585 24477237 17075344 16479376 21289288

Immunoprecipitation Pubmed IDs :

2670955

Function Blocking Pubmed IDs :

2670955

FACS Pubmed IDs :

10540182

Additional Information :

CG1 recognizes the fast-migrating component of gizzard tropomyosin, skeletal beta-tropomyosin and alpha-tropomyosin, and chicken embryo fibroblasts tropomyosin. Recognizes Human and rat Tm1 (encoded by TPM2) and cross-reacts with Tm4 (TPM4) on western blots [PMID: 22069507], and has been reported to be specific to Tpm2.1 [PMID: 27112294; 26147585].

DSHB Growth Medium :

Iscove's

References (legacy) :

Hybridoma 4(3), 223-242.; J. Cell Biol. 106, 385-393.; J. Cell Biol. 109, 1141-1152.; Biochim. Biophys. Acta. 1162(3), 255-265.; Gastroenterology 114(5), 912-922.

more info or order :

Developmental Studies Hybridoma Bank product webpage