product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
integrin betaPS (myospheroid)
catalog :
CF.6G11
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
CF.6G11
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS, immunohistochemistry - paraffin section
citations: 53
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; fruit fly ; 1:20; fig 2b
Heiden S, Siwek R, Lotz M, Borkowsky S, Schroter R, Nedvetsky P, et al. Apical-basal polarity regulators are essential for slit diaphragm assembly and endocytosis in Drosophila nephrocytes. Cell Mol Life Sci. 2021;78:3657-3672 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:10; loading ...; fig 6d'
Tattikota S, Cho B, Liu Y, Hu Y, Barrera V, Steinbaugh M, et al. A single-cell survey of Drosophila blood. elife. 2020;9: pubmed publisher
  • immunohistochemistry; fruit fly ; 1:30; loading ...; fig 3A
Raza Q, Vanderploeg J, Jacobs J. Matrix Metalloproteinases are required for membrane motility and lumenogenesis during Drosophila heart development. PLoS ONE. 2017;12:e0171905 pubmed publisher
  • western blot; fruit fly ; 1:100; fig 8
Vanhoutte D, Schips T, Kwong J, Davis J, Tjondrokoesoemo A, Brody M, et al. Thrombospondin expression in myofibers stabilizes muscle membranes. elife. 2016;5: pubmed publisher
  • immunohistochemistry; fruit fly ; 1:40; loading ...; fig 2e
Hanlon C, Andrew D. Drosophila FoxL1 non-autonomously coordinates organ placement during embryonic development. Dev Biol. 2016;419:273-284 pubmed publisher
  • immunohistochemistry - paraffin section; fruit fly ; 1:50; fig 3
Zappia M, Frolov M. E2F function in muscle growth is necessary and sufficient for viability in Drosophila. Nat Commun. 2016;7:10509 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:2; loading ...; tbl 3
Flores Benitez D, Knust E. Crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in Drosophila. elife. 2015;4: pubmed publisher
  • immunohistochemistry; fruit fly ; 1:20; fig s6
Campbell K, Casanova J. A role for E-cadherin in ensuring cohesive migration of a heterogeneous population of non-epithelial cells. Nat Commun. 2015;6:7998 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:100; fig 3
Castanieto A, Johnston M, Nystul T. EGFR signaling promotes self-renewal through the establishment of cell polarity in Drosophila follicle stem cells. elife. 2014;3: pubmed publisher
Mlih M, Karpac J. Integrin-ECM interactions and membrane-associated Catalase cooperate to promote resilience of the Drosophila intestinal epithelium. PLoS Biol. 2022;20:e3001635 pubmed publisher
Yang D, Choi K. Suppression of Patronin deficiency by altered Hippo signaling in Drosophila organ development. Cell Death Differ. 2021;28:233-250 pubmed publisher
Thuveson M, Gaengel K, Collu G, Chin M, Singh J, Mlodzik M. Integrins are required for synchronous ommatidial rotation in the Drosophila eye linking planar cell polarity signalling to the extracellular matrix. Open Biol. 2019;9:190148 pubmed publisher
Lu Y, Yao Y, Li Z. Ectopic Dpp signaling promotes stem cell competition through EGFR signaling in the Drosophila testis. Sci Rep. 2019;9:6118 pubmed publisher
Sui L, Alt S, Weigert M, Dye N, Eaton S, Jug F, et al. Differential lateral and basal tension drive folding of Drosophila wing discs through two distinct mechanisms. Nat Commun. 2018;9:4620 pubmed publisher
Park S, Lee C, Lee J, Park J, Roshandell M, Brennan C, et al. Requirement for and polarized localization of integrin proteins during Drosophila wound closure. Mol Biol Cell. 2018;29:2137-2147 pubmed publisher
Ratheesh A, Biebl J, Veselá J, Smutny M, Papusheva E, Krens S, et al. Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration. Dev Cell. 2018;45:331-346.e7 pubmed publisher
Beati H, Peek I, Hordowska P, Honemann Capito M, Glashauser J, Renschler F, et al. The adherens junction-associated LIM domain protein Smallish regulates epithelial morphogenesis. J Cell Biol. 2018;217:1079-1095 pubmed publisher
Tenenbaum C, Misra M, Alizzi R, Gavis E. Enclosure of Dendrites by Epidermal Cells Restricts Branching and Permits Coordinated Development of Spatially Overlapping Sensory Neurons. Cell Rep. 2017;20:3043-3056 pubmed publisher
Poe A, Tang L, Wang B, Li Y, Sapar M, Han C. Dendritic space-filling requires a neuronal type-specific extracellular permissive signal in Drosophila. Proc Natl Acad Sci U S A. 2017;114:E8062-E8071 pubmed publisher
Weitkunat M, Brasse M, Bausch A, Schnorrer F. Mechanical tension and spontaneous muscle twitching precede the formation of cross-striated muscle in vivo. Development. 2017;144:1261-1272 pubmed publisher
Wang Z, Rabouille C, Geisbrecht E. Loss of a Clueless-dGRASP complex results in ER stress and blocks Integrin exit from the perinuclear endoplasmic reticulum in Drosophila larval muscle. Biol Open. 2015;4:636-48 pubmed publisher
Drechsler M, Schmidt A, Meyer H, Paululat A. The conserved ADAMTS-like protein lonely heart mediates matrix formation and cardiac tissue integrity. PLoS Genet. 2013;9:e1003616 pubmed publisher
Matsuda S, Blanco J, Shimmi O. A feed-forward loop coupling extracellular BMP transport and morphogenesis in Drosophila wing. PLoS Genet. 2013;9:e1003403 pubmed publisher
Lewellyn L, Cetera M, Horne Badovinac S. Misshapen decreases integrin levels to promote epithelial motility and planar polarity in Drosophila. J Cell Biol. 2013;200:721-9 pubmed publisher
Cohen J, Brinton E, Ito M, Jacobson T. Understanding Statin Use in America and Gaps in Patient Education (USAGE): an internet-based survey of 10,138 current and former statin users. J Clin Lipidol. 2012;6:208-15 pubmed publisher
Elias M, Pronovost S, Cahill K, Beckerle M, Kadrmas J. A crucial role for Ras suppressor-1 (RSU-1) revealed when PINCH and ILK binding is disrupted. J Cell Sci. 2012;125:3185-94 pubmed publisher
Lahaye L, Wouda R, de Jong A, Fradkin L, Noordermeer J. WNT5 interacts with the Ryk receptors doughnut and derailed to mediate muscle attachment site selection in Drosophila melanogaster. PLoS ONE. 2012;7:e32297 pubmed publisher
Kim M, Shrestha B, Blazeski R, Mason C, Grueber W. Integrins establish dendrite-substrate relationships that promote dendritic self-avoidance and patterning in drosophila sensory neurons. Neuron. 2012;73:79-91 pubmed publisher
Pereira A, Tudor C, Kanger J, Subramaniam V, Martin Blanco E. Integrin-dependent activation of the JNK signaling pathway by mechanical stress. PLoS ONE. 2011;6:e26182 pubmed publisher
Xavier M, Williams M. The Rho-family GTPase Rac1 regulates integrin localization in Drosophila immunosurveillance cells. PLoS ONE. 2011;6:e19504 pubmed publisher
Kugler S, Gehring E, Wallkamm V, Krüger V, Nagel A. The Putzig-NURF nucleosome remodeling complex is required for ecdysone receptor signaling and innate immunity in Drosophila melanogaster. Genetics. 2011;188:127-39 pubmed publisher
Egoz Matia N, Nachman A, Halachmi N, Toder M, Klein Y, Salzberg A. Spatial regulation of cell adhesion in the Drosophila wing is mediated by Delilah, a potent activator of βPS integrin expression. Dev Biol. 2011;351:99-109 pubmed publisher
Gandille P, Narbonne Reveau K, Boissonneau E, Randsholt N, Busson D, Pret A. Mutations in the polycomb group gene polyhomeotic lead to epithelial instability in both the ovary and wing imaginal disc in Drosophila. PLoS ONE. 2010;5:e13946 pubmed publisher
Ueyama M, Akimoto Y, Ichimiya T, Ueda R, Kawakami H, Aigaki T, et al. Increased apoptosis of myoblasts in Drosophila model for the Walker-Warburg syndrome. PLoS ONE. 2010;5:e11557 pubmed publisher
Shirinian M, Popovic M, Grabbe C, Varshney G, Hugosson F, Bos H, et al. The Rap1 guanine nucleotide exchange factor C3G is required for preservation of larval muscle integrity in Drosophila melanogaster. PLoS ONE. 2010;5:e9403 pubmed publisher
Fraichard S, Bouge A, Kendall T, Chauvel I, Bouhin H, Bunch T. Tenectin is a novel alphaPS2betaPS integrin ligand required for wing morphogenesis and male genital looping in Drosophila. Dev Biol. 2010;340:504-17 pubmed publisher
Klein Y, Halachmi N, Egoz Matia N, Toder M, Salzberg A. The proprioceptive and contractile systems in Drosophila are both patterned by the EGR family transcription factor Stripe. Dev Biol. 2010;337:458-70 pubmed publisher
Viktorinova I, König T, Schlichting K, Dahmann C. The cadherin Fat2 is required for planar cell polarity in the Drosophila ovary. Development. 2009;136:4123-32 pubmed publisher
Delon I, Brown N. The integrin adhesion complex changes its composition and function during morphogenesis of an epithelium. J Cell Sci. 2009;122:4363-74 pubmed publisher
Abe M, Setoguchi Y, Tanaka T, Awano W, Takahashi K, Ueda R, et al. Membrane protein location-dependent regulation by PI3K (III) and rabenosyn-5 in Drosophila wing cells. PLoS ONE. 2009;4:e7306 pubmed publisher
Woolworth J, Nallamothu G, Hsu T. The Drosophila metastasis suppressor gene Nm23 homolog, awd, regulates epithelial integrity during oogenesis. Mol Cell Biol. 2009;29:4679-90 pubmed publisher
Rodahl L, Haglund K, Sem Jacobsen C, Wendler F, Vincent J, Lindmo K, et al. Disruption of Vps4 and JNK function in Drosophila causes tumour growth. PLoS ONE. 2009;4:e4354 pubmed publisher
Dinkins M, Fratto V, LeMosy E. Integrin alpha chains exhibit distinct temporal and spatial localization patterns in epithelial cells of the Drosophila ovary. Dev Dyn. 2008;237:3927-39 pubmed publisher
Bahri S, Choy J, Manser E, Lim L, Yang X. The Drosophila homologue of Arf-GAP GIT1, dGIT, is required for proper muscle morphogenesis and guidance during embryogenesis. Dev Biol. 2009;325:15-23 pubmed publisher
Helsten T, Bunch T, Kato H, Yamanouchi J, Choi S, Jannuzi A, et al. Differences in regulation of Drosophila and vertebrate integrin affinity by talin. Mol Biol Cell. 2008;19:3589-98 pubmed publisher
Jani K, Schock F. Zasp is required for the assembly of functional integrin adhesion sites. J Cell Biol. 2007;179:1583-97 pubmed publisher
Kadrmas J, Smith M, Clark K, Pronovost S, Muster N, Yates J, et al. The integrin effector PINCH regulates JNK activity and epithelial migration in concert with Ras suppressor 1. J Cell Biol. 2004;167:1019-24 pubmed
Grabbe C, Zervas C, Hunter T, Brown N, Palmer R. Focal adhesion kinase is not required for integrin function or viability in Drosophila. Development. 2004;131:5795-805 pubmed
Araujo H, Negreiros E, Bier E. Integrins modulate Sog activity in the Drosophila wing. Development. 2003;130:3851-64 pubmed
Hoang B, Chiba A. Genetic analysis on the role of integrin during axon guidance in Drosophila. J Neurosci. 1998;18:7847-55 pubmed
Gullberg D, Fessler L, Fessler J. Differentiation, extracellular matrix synthesis, and integrin assembly by Drosophila embryo cells cultured on vitronectin and laminin substrates. Dev Dyn. 1994;199:116-28 pubmed
Brower D, Wilcox M, Piovant M, Smith R, Reger L. Related cell-surface antigens expressed with positional specificity in Drosophila imaginal discs. Proc Natl Acad Sci U S A. 1984;81:7485-9 pubmed
Brower D, Piovant M, Reger L. Developmental analysis of Drosophila position-specific antigens. Dev Biol. 1985;108:120-30 pubmed
product information
Internal ID :
1376
Name :
CF.6G11
Depositor Name :
Brower, D.
Depositor Institution :
Harvard Medical School
Date Deposited :
7/17/02
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
integrin betaPS (myospheroid)
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG2b, kappa light chain
Isotype for catalog (legacy) :
IgG2b, kappa light chain
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
6390440
Depositor Notes (Special Instructions) :
This antibody doesn't block function.
Collections :
Cell adhesion,Cell signaling,Drosophila antigens,Neurodevelopment,Skeletal muscle
Search Keywords :
Brower, D., integrin betaPS (myospheroid), Drosophila, MIgG2b, kappa light chain, Drosophila, mys, betaPS, CG1560, AB_528310, Monoclonal, Neurodevelopment/Cell adhesion/Cell signaling/Drosophila antigens/Skeletal muscle, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/FACS
Antigen Molecular Weight :
93 kDa
Gene :
mys
Alternate Gene Name(s) :
betaPS, CG1560
Uniprot ID :
P11584
Antibody Registry ID :
AB_528310
Synonyms (Alternate Clone Names) :
PS3, betaPS
Immunogen :
Sonicated imaginal discs
Immunogen Sequence :
Total protein
Clonality :
Monoclonal
Myeloma Strain :
P3/NSI
Epitope Mapped :
No
Recommended Applications :
FACS,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
9742153
Immunofluorescence Pubmed IDs :
6390440 18996366 19906848 19581292 19194501 19798413 19903692 19944090 20209136 20644630 21085656 21215259 21385730 21603603 22467865 7515725 12835400 25862246 23509067 15525665 19035354 22243748 3918898 27669143 28874572 29738712 30397306 30992503 31409231
Western Blot Pubmed IDs :
22467865 27669143
Immunoprecipitation Pubmed IDs :
7515725 12835400
FACS Pubmed IDs :
18508915
Additional Information :
Integrins are heterodimers of an alpha and a beta subunit. Beta-PS associates with either alpha-PS1, alpha-PS2, alpha-PS3, alpha-PS4 or alpha-PS5.
DSHB Growth Medium :
RPMI
References (legacy) :
Proc. Natl. Acad. Sci. 81, 7485-7489.; Dev. Biol. 325(1), 15-23.; Development 136, 4123-4132.; Mole. Cell. Biol. 29(17), 4679-4690.; PLoS ONE 4(2): e4354. doi:10.1371/journal.pone.0004354.; PLoS ONE 4(10): e7306. doi:10.1371/journal.pone.0007306.; J. Cell Sci. 122(23), 4363-4374.; Dev. Biol. 337(2), 458-470.; Dev. Biol. 340(2), 504-517.; PLoS ONE 5(3): e9403. doi:10.1371/journal.pone.0009403.; PLoS ONE 5(7): e11557. doi:10.1371/journal.pone.0011557.; PLoS ONE 5(11): e13946. doi:10.1371/journal.pone.0013946.; Dev. Biol. 351(1), 99-109.; doi: 10.1534/genetics.111.127795.; PLos ONE 6(5): e19504. doi:10.1371/journal.pone.0019504.; J. Cell Sci. 10138: doi:10.1242/jcs.101386.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US