CD133/ Prominin-1 | Developmental Studies Hybridoma Bank CD133 HB#7 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

CD133/ Prominin-1

catalog :

CD133 HB#7

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CD133 HB#7

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 3

Reference
Görgens A, Ludwig A, Möllmann M, Krawczyk A, Dürig J, Hanenberg H, et al. Multipotent hematopoietic progenitors divide asymmetrically to create progenitors of the lymphomyeloid and erythromyeloid lineages. Stem Cell Reports. 2014;3:1058-72 pubmed publisher
Skubitz A, Taras E, Boylan K, Waldron N, Oh S, Panoskaltsis Mortari A, et al. Targeting CD133 in an in vivo ovarian cancer model reduces ovarian cancer progression. Gynecol Oncol. 2013;130:579-87 pubmed publisher
Swaminathan S, Olin M, Forster C, Cruz K, Panyam J, Ohlfest J. Identification of a novel monoclonal antibody recognizing CD133. J Immunol Methods. 2010;361:110-5 pubmed publisher

product information

Internal ID :

6701

Name :

CD133 HB#7

Depositor Name :

Swaminathan, S.K. / Panyam, J. / Ohlfest, J.R.

Depositor Institution :

University of Minnesota

Date Deposited :

8/17/10

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

CD133/ Prominin-1

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human

Species Tested (legacy) :

human

Initial Publication Pubmed ID :

20674577

Depositor Notes (Special Instructions) :

This antibody recognizes both glycosylated and non-glycoslyated extracellular domains of CD133.

Collections :

CD antigens,Human,Stem cell

Search Keywords :

Swaminathan, S.K. / Panyam, J. / Ohlfest, J.R., CD133/ Prominin-1, Human, MIgG1, Human, PROM1, AC133, CD133, CORD12, MCDR2, PROML1, RP41, STGD4, , Monoclonal, Human/Cluster determinant antigens, FACS/Immunohistochemistry/Immunofluorescence/Western blot

Antigen Molecular Weight :

predicted molecular weight 97 kDa

Gene :

PROM1

Alternate Gene Name(s) :

AC133, CD133, CORD12, MCDR2, PROML1, RP41, STGD4

Uniprot ID :

O43490

Antibody Registry ID :

AB_2722053

Synonyms (Alternate Clone Names) :

HC7

Immunogen :

Recombinant chimeric protein

Immunogen Sequence :

aa180-380 and aa612-765

Clonality :

Monoclonal

Myeloma Strain :

SP 2/0

Epitope Mapped :

Recommended Applications :

FACS,Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

20674577

Immunofluorescence Pubmed IDs :

20674577 25448068

Western Blot Pubmed IDs :

20674577 28923841

FFPE Pubmed IDs :

20674577

FACS Pubmed IDs :

20674577 23721800 25448068

Additional Information :

Depending upon glycosylation, the apparent molecular weight on western blots will increase to ~ 120 kDa. This antibody recognizes CD133 is aldehyde fixed cells and tissues (see 25448068 ). RRID:AB_2722053

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Immunol. Methods, doi:10.1016/j.jim.2010.07.007.

more info or order :

Developmental Studies Hybridoma Bank product webpage