Notch2 intracellular domain (human) | Developmental Studies Hybridoma Bank C651.6DbHN product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Notch2 intracellular domain (human)

catalog :

C651.6DbHN

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

C651.6DbHN

reactivity :

human, mouse, dog

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 29

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; fig s4a	Mangolini M, Götte F, Moore A, Ammon T, Oelsner M, Lutzny Geier G, et al. Notch2 controls non-autonomous Wnt-signalling in chronic lymphocytic leukaemia. Nat Commun. 2018;9:3839 pubmed publisher
western blot; mouse; loading ...; fig 2e	Hwang I, Boularan C, Harrison K, Kehrl J. Gαi Signaling Promotes Marginal Zone B Cell Development by Enabling Transitional B Cell ADAM10 Expression. Front Immunol. 2018;9:687 pubmed publisher
western blot; mouse; loading ...; fig 4a	Fujiwara T, Zhou J, Ye S, Zhao H. RNA-binding protein Musashi2 induced by RANKL is critical for osteoclast survival. Cell Death Dis. 2016;7:e2300 pubmed publisher
western blot; mouse; 1:500; fig 5 western blot; human; 1:1000; fig 2	Dhar S, Kumar A, Zhang L, Rimando A, Lage J, Lewin J, et al. Dietary pterostilbene is a novel MTA1-targeted chemopreventive and therapeutic agent in prostate cancer. Oncotarget. 2016;7:18469-84 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200	DeFalco T, Saraswathula A, Briot A, Iruela Arispe M, Capel B. Testosterone levels influence mouse fetal Leydig cell progenitors through notch signaling. Biol Reprod. 2013;88:91 pubmed publisher
	Azimi M, Le T, Brown N. Presenilin gene function and Notch signaling feedback regulation in the developing mouse lens. Differentiation. 2018;102:40-52 pubmed publisher
	Yu J, Zanotti S, Walia B, JELLISON E, Sanjay A, Canalis E. The Hajdu Cheney Mutation Is a Determinant of B-Cell Allocation of the Splenic Marginal Zone. Am J Pathol. 2018;188:149-159 pubmed publisher
	Fiorotto R, Amenduni M, Mariotti V, Fabris L, Spirli C, Strazzabosco M. Src kinase inhibition reduces inflammatory and cytoskeletal changes in ΔF508 human cholangiocytes and improves cystic fibrosis transmembrane conductance regulator correctors efficacy. Hepatology. 2018;67:972-988 pubmed publisher
	Fortini F, Vieceli Dalla Sega F, Caliceti C, Aquila G, Pannella M, Pannuti A, et al. Estrogen receptor β-dependent Notch1 activation protects vascular endothelium against tumor necrosis factor α (TNFα)-induced apoptosis. J Biol Chem. 2017;292:18178-18191 pubmed publisher
	Zhang S, Chung W, Xu K. Lunatic Fringe is a potent tumor suppressor in Kras-initiated pancreatic cancer. Oncogene. 2016;35:2485-95 pubmed publisher
	Bauer L, Takacs A, Slotta Huspenina J, Langer R, Becker K, Novotny A, et al. Clinical Significance of NOTCH1 and NOTCH2 Expression in Gastric Carcinomas: An Immunohistochemical Study. Front Oncol. 2015;5:94 pubmed publisher
	Zhou J, Fujiwara T, Ye S, Li X, Zhao H. Ubiquitin E3 Ligase LNX2 is Critical for Osteoclastogenesis In Vitro by Regulating M-CSF/RANKL Signaling and Notch2. Calcif Tissue Int. 2015;96:465-75 pubmed publisher
	Baumgart A, Mazur P, Anton M, Rudelius M, Schwamborn K, Feuchtinger A, et al. Opposing role of Notch1 and Notch2 in a Kras(G12D)-driven murine non-small cell lung cancer model. Oncogene. 2015;34:578-88 pubmed publisher
	Simonetti G, Carette A, Silva K, Wang H, De Silva N, Heise N, et al. IRF4 controls the positioning of mature B cells in the lymphoid microenvironments by regulating NOTCH2 expression and activity. J Exp Med. 2013;210:2887-902 pubmed publisher
	Caliceti C, Aquila G, Pannella M, Morelli M, Fortini C, Pinton P, et al. 17Î²-estradiol enhances signalling mediated by VEGF-A-delta-like ligand 4-notch1 axis in human endothelial cells. PLoS ONE. 2013;8:e71440 pubmed publisher
	Bauer L, Langer R, Becker K, Hapfelmeier A, Ott K, Novotny A, et al. Expression profiling of stem cell-related genes in neoadjuvant-treated gastric cancer: a NOTCH2, GSK3B and ?-catenin gene signature predicts survival. PLoS ONE. 2012;7:e44566 pubmed publisher
	Tu X, Chen J, Lim J, Karner C, Lee S, Heisig J, et al. Physiological notch signaling maintains bone homeostasis via RBPjk and Hey upstream of NFATc1. PLoS Genet. 2012;8:e1002577 pubmed publisher
	Mazur P, Riener M, Jochum W, Kristiansen G, Weber A, Schmid R, et al. Expression and clinicopathological significance of notch signaling and cell-fate genes in biliary tract cancer. Am J Gastroenterol. 2012;107:126-35 pubmed publisher
	Zeuner A, Francescangeli F, Signore M, Venneri M, Pedini F, Felli N, et al. The Notch2-Jagged1 interaction mediates stem cell factor signaling in erythropoiesis. Cell Death Differ. 2011;18:371-80 pubmed publisher
	Watanabe K, Nagaoka T, Lee J, Bianco C, Gonzales M, Castro N, et al. Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1. J Cell Biol. 2009;187:343-53 pubmed publisher
	Ellsworth B, Butts D, Camper S. Mechanisms underlying pituitary hypoplasia and failed cell specification in Lhx3-deficient mice. Dev Biol. 2008;313:118-29 pubmed
	Brinkmeier M, Potok M, Davis S, Camper S. TCF4 deficiency expands ventral diencephalon signaling and increases induction of pituitary progenitors. Dev Biol. 2007;311:396-407 pubmed
	Raetzman L, Cai J, Camper S. Hes1 is required for pituitary growth and melanotrope specification. Dev Biol. 2007;304:455-66 pubmed
	Noggle S, Weiler D, Condie B. Notch signaling is inactive but inducible in human embryonic stem cells. Stem Cells. 2006;24:1646-53 pubmed
	Tanimizu N, Miyajima A. Notch signaling controls hepatoblast differentiation by altering the expression of liver-enriched transcription factors. J Cell Sci. 2004;117:3165-74 pubmed
	Raetzman L, Ross S, Cook S, Dunwoodie S, Camper S, Thomas P. Developmental regulation of Notch signaling genes in the embryonic pituitary: Prop1 deficiency affects Notch2 expression. Dev Biol. 2004;265:329-40 pubmed
	Nefedova Y, Cheng P, Alsina M, Dalton W, Gabrilovich D. Involvement of Notch-1 signaling in bone marrow stroma-mediated de novo drug resistance of myeloma and other malignant lymphoid cell lines. Blood. 2004;103:3503-10 pubmed
	Blaumueller C, Qi H, Zagouras P, Artavanis Tsakonas S. Intracellular cleavage of Notch leads to a heterodimeric receptor on the plasma membrane. Cell. 1997;90:281-91 pubmed
	Zagouras P, Stifani S, Blaumueller C, Carcangiu M, Artavanis Tsakonas S. Alterations in Notch signaling in neoplastic lesions of the human cervix. Proc Natl Acad Sci U S A. 1995;92:6414-8 pubmed

product information

Internal ID :

276

Name :

C651.6DbHN

Depositor Name :

Artavanis-Tsakonas, S.

Depositor Institution :

Harvard Medical School

Date Deposited :

5/2/00

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Notch2 intracellular domain (human)

Antigen Species :

Human

Host Species :

rat

Isotype :

RIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Canine,Human,Mouse

Species Tested (legacy) :

human, mouse, dog

Initial Publication Pubmed ID :

7604005

Depositor Notes (Special Instructions) :

Works well for Immunoprecipitation

Collections :

Cardiac development,Cell adhesion,Cell signaling,Human,Transcription factors

Search Keywords :

Artavanis-Tsakonas, S., Notch2 intracellular domain (human), Human, RIgG1, kappa light chain, Human/mouse/canine, Neurogenic locus notch homolog protein 2, NOTCH2, hN2, N2ICD, AGS2, HJCYS, AB_528412, monoclonal, Cardiac development/Cell adhesion/Cell signaling/Human/Transcription factors, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Apparent: 300 kDa processed to give a major intracellular product of 110 kDa

Gene :

NOTCH2

Alternate Gene Name(s) :

hN2; AGS2; HJCYS

Uniprot ID :

Q04721

Antibody Registry ID :

AB_528412

Synonyms (Alternate Clone Names) :

6D, bhN6D

Immunogen :

Recombinant fusion protein GST-Notch2 intrcellular domain

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

Ag8/ATCC CRL1580

Epitope Mapped :

Yes

Epitope Location or Sequence :

intracellular domain

Epitope Map PubMed ID :

7604005

Recommended Applications :

FACS,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Excellent

Immunohistochemistry Pubmed IDs :

7604005 24509876 26279302 21931375 15226394

Immunofluorescence Pubmed IDs :

7604005 17919533 18037398 14732396 25712254

Western Blot Pubmed IDs :

7604005 9244302 23967210 14670925 24509876 16614005 26279302 20829885 31371452

FACS Pubmed IDs :

20829885

DSHB Growth Medium :

RPMI

References (legacy) :

Proc. Natl. Acad. Sci. 92, 6414-6418.; Cell 90, 281-291.; Dev. Biol. 304, 455-466.; Dev. Biol. 311, 396-407.; Dev. Biol. 313, 118-129.; PLoS One 8(8): e71440. doi:10.1371/journal.pone.0071440.

more info or order :

Developmental Studies Hybridoma Bank product webpage