Coracle (heavy isoform) | Developmental Studies Hybridoma Bank C615.16 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Coracle (heavy isoform)

catalog :

C615.16

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C615.16

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 21

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; 1:500	Fairchild M, Smendziuk C, Tanentzapf G. A somatic permeability barrier around the germline is essential for Drosophila spermatogenesis. Development. 2015;142:268-81 pubmed publisher
	Tsarouhas V, Liu D, Tsikala G, Fedoseienko A, Zinn K, Matsuda R, et al. WASH phosphorylation balances endosomal versus cortical actin network integrities during epithelial morphogenesis. Nat Commun. 2019;10:2193 pubmed publisher
	Samarasekera G, Auld V. C-terminal Src kinase (Csk) regulates the tricellular junction protein Gliotactin independent of Src. Mol Biol Cell. 2018;29:123-136 pubmed publisher
	Tenenbaum C, Misra M, Alizzi R, Gavis E. Enclosure of Dendrites by Epidermal Cells Restricts Branching and Permits Coordinated Development of Spatially Overlapping Sensory Neurons. Cell Rep. 2017;20:3043-3056 pubmed publisher
	Liang J, Balachandra S, Ngo S, O Brien L. Feedback regulation of steady-state epithelial turnover and organ size. Nature. 2017;548:588-591 pubmed publisher
	Izumi Y, Motoishi M, Furuse K, Furuse M. A tetraspanin regulates septate junction formation in Drosophila midgut. J Cell Sci. 2016;129:1155-64 pubmed publisher
	Sollier K, GaudÃ© H, Chartier F, Laprise P. Rac1 controls epithelial tube length through the apical secretion and polarity pathways. Biol Open. 2015;5:49-54 pubmed publisher
	Kim M, Choe K. Basement membrane and cell integrity of self-tissues in maintaining Drosophila immunological tolerance. PLoS Genet. 2014;10:e1004683 pubmed publisher
	Tsikala G, Karagogeos D, Strigini M. Btk-dependent epithelial cell rearrangements contribute to the invagination of nearby tubular structures in the posterior spiracles of Drosophila. Dev Biol. 2014;396:42-56 pubmed publisher
	Maimon I, Popliker M, Gilboa L. Without children is required for Stat-mediated zfh1 transcription and for germline stem cell differentiation. Development. 2014;141:2602-10 pubmed publisher
	Nakajima Y, Meyer E, Kroesen A, McKinney S, Gibson M. Epithelial junctions maintain tissue architecture by directing planar spindle orientation. Nature. 2013;500:359-62 pubmed publisher
	Mayhew M, Merritt D. The morphogenesis of spermathecae and spermathecal glands in Drosophila melanogaster. Arthropod Struct Dev. 2013;42:385-93 pubmed publisher
	Song Y, Eng M, Ghabrial A. Focal defects in single-celled tubes mutant for Cerebral cavernous malformation 3, GCKIII, or NSF2. Dev Cell. 2013;25:507-19 pubmed publisher
	Izumi Y, Yanagihashi Y, Furuse M. A novel protein complex, Mesh-Ssk, is required for septate junction formation in the Drosophila midgut. J Cell Sci. 2012;125:4923-33 pubmed publisher
	Ile K, Tripathy R, Goldfinger V, Renault A. Wunen, a Drosophila lipid phosphate phosphatase, is required for septate junction-mediated barrier function. Development. 2012;139:2535-46 pubmed publisher
	Yanagihashi Y, Usui T, Izumi Y, Yonemura S, Sumida M, Tsukita S, et al. Snakeskin, a membrane protein associated with smooth septate junctions, is required for intestinal barrier function in Drosophila. J Cell Sci. 2012;125:1980-90 pubmed publisher
	Kim M, Shrestha B, Blazeski R, Mason C, Grueber W. Integrins establish dendrite-substrate relationships that promote dendritic self-avoidance and patterning in drosophila sensory neurons. Neuron. 2012;73:79-91 pubmed publisher
	Kim M, McGinnis W. Phosphorylation of Grainy head by ERK is essential for wound-dependent regeneration but not for development of an epidermal barrier. Proc Natl Acad Sci U S A. 2011;108:650-5 pubmed publisher
	Tanentzapf G, Tepass U. Interactions between the crumbs, lethal giant larvae and bazooka pathways in epithelial polarization. Nat Cell Biol. 2003;5:46-52 pubmed
	Fernandez R, Takahashi F, Liu Z, Steward R, Stein D, Stanley E. The Drosophila shark tyrosine kinase is required for embryonic dorsal closure. Genes Dev. 2000;14:604-14 pubmed
	Fehon R, Dawson I, Artavanis Tsakonas S. A Drosophila homologue of membrane-skeleton protein 4.1 is associated with septate junctions and is encoded by the coracle gene. Development. 1994;120:545-57 pubmed

product information

Internal ID :

3043

Name :

C615.16

Depositor Name :

Fehon, R.

Depositor Institution :

University of Chicago

Depositor Website :

http://fehonlab.bsd.uchicago.edu/

Date Deposited :

8/25/07

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Coracle (heavy isoform)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

8162854

Collections :

Cell signaling,Cytoskeleton,Drosophila antigens,Skeletal muscle

Search Keywords :

Richard Fehon, Coracle , Drosophila, MIgG1, kappa light chain, Drosophila, cora, CG11949 , AB_1161644, Monoclonal, Drosophila antigens/Cytoskeletal elements, Immunohistochemistry/Immunofluorescence/Western blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 184 kDa; Apparent: 200 kDa

Gene :

cora

Alternate Gene Name(s) :

CG11949, Cor, Coracle, D4.1

Uniprot ID :

Q9V8R9

Antibody Registry ID :

AB_1161644

Immunogen :

Recombinant GST fusion protein

Immunogen Sequence :

Coralcle C-terminal region a.a. 608-1285

Clonality :

Monoclonal

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 608-1285 of isoform 1 (Uniprot ID: Q9V8R9-1).

Epitope Map PubMed ID :

8162854

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

10716948

Immunofluorescence Pubmed IDs :

25329560 24903753 23873041 22243748 21187384 23872109 22854041 25305143 26848177 25329560 22328496 26700724 25503408 22675212 23763949 12510193 29167383 29706514 30389853 31006568 31390561

Western Blot Pubmed IDs :

26700724

Additional Information :

This antibody only recognizes coracle isoform 1 (Uniprot ID: Q9V8R9-1). This antigen is also known as protein 4.1 homolog and is a component of septate junctions. FBgn0010434 / RRID:AB_1161644

DSHB Growth Medium :

Iscove's

References (legacy) :

Development 120, 545-557.; J. Cell Biol. 140, 1463-1473.; PNAS 108(2), 650-655.

more info or order :

Developmental Studies Hybridoma Bank product webpage