Delta, extracellular domain | Developmental Studies Hybridoma Bank C594.9B product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Delta, extracellular domain

catalog :

C594.9B

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C594.9B

reactivity :

Drosophila melanogaster

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 51

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; Drosophila melanogaster; 1:50; loading ...; fig 4c	Jörg D, Caygill E, Hakes A, Contreras E, Brand A, Simons B. The proneural wave in the Drosophila optic lobe is driven by an excitable reaction-diffusion mechanism. elife. 2019;8: pubmed publisher
immunocytochemistry; Drosophila melanogaster; 1:100; loading ...; fig 1a	Zhou J, Edgar B, Boutros M. ATF3 acts as a rheostat to control JNK signalling during intestinal regeneration. Nat Commun. 2017;8:14289 pubmed publisher
immunohistochemistry; Drosophila melanogaster; 1:2000; fig 1e	Siudeja K, Nassari S, Gervais L, Skorski P, Lameiras S, Stolfa D, et al. Frequent Somatic Mutation in Adult Intestinal Stem Cells Drives Neoplasia and Genetic Mosaicism during Aging. Cell Stem Cell. 2015;17:663-74 pubmed publisher
immunocytochemistry; Drosophila melanogaster; 1:500; fig 1	Zacharogianni M, Aguilera Gomez A, Veenendaal T, Smout J, Rabouille C. A stress assembly that confers cell viability by preserving ERES components during amino-acid starvation. elife. 2014;3: pubmed publisher
	Zhao S, Wu C, Gao Z, Li X, Guo Z, Wang Z. Notch signaling governs the expression of glypican Dally to define the stem cell niche. Biol Open. 2020;9: pubmed publisher
	Bäumers M, Klose S, Brüser C, Haag C, Hänsch S, Pannen H, et al. The auxiliary ESCRT complexes provide robustness to cold in poikilothermic organisms. Biol Open. 2019;8: pubmed publisher
	Gültekin Y, Steller H. Axin proteolysis by Iduna is required for the regulation of stem cell proliferation and intestinal homeostasis in Drosophila. Development. 2019;146: pubmed publisher
	Hunter G, He L, Perrimon N, Charras G, Giniger E, Baum B. A role for actomyosin contractility in Notch signaling. BMC Biol. 2019;17:12 pubmed publisher
	Li Y, Liu T, Zhang J. The ATPase TER94 regulates Notch signaling during Drosophila wing development. Biol Open. 2019;8: pubmed publisher
	Cara F, Bülow M, Simmonds A, Rachubinski R. Dysfunctional peroxisomes compromise gut structure and host defense by increased cell death and Tor-dependent autophagy. Mol Biol Cell. 2018;29:2766-2783 pubmed publisher
	Li B, Wong C, Gao S, Zhang R, Sun R, Li Y, et al. The retromer complex safeguards against neural progenitor-derived tumorigenesis by regulating Notch receptor trafficking. elife. 2018;7: pubmed publisher
	Yamakawa T, Atsumi Y, Kubo S, Yamagishi A, Morita I, Matsuno K. Insight into Notch Signaling Steps That Involve pecanex from Dominant-Modifier Screens in Drosophila. Genetics. 2018;209:1099-1119 pubmed publisher
	Matamoro Vidal A, Huang Y, Salazar Ciudad I, Shimmi O, Houle D. Quantitative Morphological Variation in the Developing Drosophila Wing. G3 (Bethesda). 2018;8:2399-2409 pubmed publisher
	He L, Si G, Huang J, Samuel A, Perrimon N. Mechanical regulation of stem-cell differentiation by the stretch-activated Piezo channel. Nature. 2018;555:103-106 pubmed publisher
	Chen J, Xu N, Wang C, Huang P, Huang H, Jin Z, et al. Transient Scute activation via a self-stimulatory loop directs enteroendocrine cell pair specification from self-renewing intestinal stem cells. Nat Cell Biol. 2018;20:152-161 pubmed publisher
	Zhang L, Turner B, Ribbeck K, Ten Hagen K. Loss of the mucosal barrier alters the progenitor cell niche via Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling. J Biol Chem. 2017;292:21231-21242 pubmed publisher
	Sallé J, Gervais L, Boumard B, Stefanutti M, Siudeja K, Bardin A. Intrinsic regulation of enteroendocrine fate by Numb. EMBO J. 2017;36:1928-1945 pubmed publisher
	Singh S, Zeng X, Zhao J, Liu Y, Hou G, Liu H, et al. The lipolysis pathway sustains normal and transformed stem cells in adult Drosophila. Nature. 2016;538:109-113 pubmed publisher
	Troost T, Schneider M, Klein T. A re-examination of the selection of the sensory organ precursor of the bristle sensilla of Drosophila melanogaster. PLoS Genet. 2015;11:e1004911 pubmed publisher
	Palmer W, Jia D, Deng W. Cis-interactions between Notch and its ligands block ligand-independent Notch activity. elife. 2014;3: pubmed publisher
	Driver I, Ohlstein B. Specification of regional intestinal stem cell identity during Drosophila metamorphosis. Development. 2014;141:1848-56 pubmed publisher
	Guo Z, Driver I, Ohlstein B. Injury-induced BMP signaling negatively regulates Drosophila midgut homeostasis. J Cell Biol. 2013;201:945-61 pubmed publisher
	Aguilar Hidalgo D, Domínguez Cejudo M, Amore G, Brockmann A, Lemos M, Cordoba A, et al. A Hh-driven gene network controls specification, pattern and size of the Drosophila simple eyes. Development. 2013;140:82-92 pubmed publisher
	Dornier E, Coumailleau F, Ottavi J, Moretti J, Boucheix C, Mauduit P, et al. TspanC8 tetraspanins regulate ADAM10/Kuzbanian trafficking and promote Notch activation in flies and mammals. J Cell Biol. 2012;199:481-96 pubmed publisher
	Liu S, Bonner J, Chanet S, Commisso C, Skwarek L, Schweisguth F, et al. Functional analysis of the NHR2 domain indicates that oligomerization of Neuralized regulates ubiquitination and endocytosis of Delta during Notch signaling. Mol Cell Biol. 2012;32:4933-45 pubmed publisher
	Daskalaki A, Shalaby N, Kux K, Tsoumpekos G, Tsibidis G, Muskavitch M, et al. Distinct intracellular motifs of Delta mediate its ubiquitylation and activation by Mindbomb1 and Neuralized. J Cell Biol. 2011;195:1017-31 pubmed publisher
	Tajiri R, Misaki K, Yonemura S, Hayashi S. Joint morphology in the insect leg: evolutionary history inferred from Notch loss-of-function phenotypes in Drosophila. Development. 2011;138:4621-6 pubmed publisher
	Quijano J, Stinchfield M, Zerlanko B, Gibbens Y, Takaesu N, Hyman Walsh C, et al. The Sno oncogene antagonizes Wingless signaling during wing development in Drosophila. PLoS ONE. 2010;5:e11619 pubmed publisher
	Benhra N, Vignaux F, Dussert A, Schweisguth F, Le Borgne R. Neuralized promotes basal to apical transcytosis of delta in epithelial cells. Mol Biol Cell. 2010;21:2078-86 pubmed publisher
	Steele L, Sukhanova M, Xu J, Gordon G, Huang Y, Yu L, et al. Retinoblastoma family protein promotes normal R8-photoreceptor differentiation in the absence of rhinoceros by inhibiting dE2F1 activity. Dev Biol. 2009;335:228-36 pubmed publisher
	Sun J, Smith L, Armento A, Deng W. Regulation of the endocycle/gene amplification switch by Notch and ecdysone signaling. J Cell Biol. 2008;182:885-96 pubmed publisher
	Delwig A, Rand M. Kuz and TACE can activate Notch independent of ligand. Cell Mol Life Sci. 2008;65:2232-43 pubmed publisher
	Cornbrooks C, Bland C, Williams D, Truman J, Rand M. Delta expression in post-mitotic neurons identifies distinct subsets of adult-specific lineages in Drosophila. Dev Neurobiol. 2007;67:23-38 pubmed
	Hagedorn E, Bayraktar J, Kandachar V, Bai T, Englert D, Chang H. Drosophila melanogaster auxilin regulates the internalization of Delta to control activity of the Notch signaling pathway. J Cell Biol. 2006;173:443-52 pubmed
	Delwig A, Bland C, Beem Miller M, Kimberly P, Rand M. Endocytosis-independent mechanisms of Delta ligand proteolysis. Exp Cell Res. 2006;312:1345-60 pubmed
	Kawakami T, Shimizu T, Kimura A, Hasegawa H, Siar C, Ng K, et al. Immunohistochemical examination of cytological differentiation in osteosarcomas. Eur J Med Res. 2005;10:475-9 pubmed
	Bardot B, Mok L, Thayer T, Ahimou F, Wesley C. The Notch amino terminus regulates protein levels and Delta-induced clustering of Drosophila Notch receptors. Exp Cell Res. 2005;304:202-23 pubmed
	Ahimou F, Mok L, Bardot B, Wesley C. The adhesion force of Notch with Delta and the rate of Notch signaling. J Cell Biol. 2004;167:1217-29 pubmed
	Mistry H, Wilson B, Roberts I, O Kane C, Skeath J. Cullin-3 regulates pattern formation, external sensory organ development and cell survival during Drosophila development. Mech Dev. 2004;121:1495-507 pubmed
	Kamimura K, Rhodes J, Ueda R, McNeely M, Shukla D, Kimata K, et al. Regulation of Notch signaling by Drosophila heparan sulfate 3-O sulfotransferase. J Cell Biol. 2004;166:1069-79 pubmed
	Kondylis V, Rabouille C. A novel role for dp115 in the organization of tER sites in Drosophila. J Cell Biol. 2003;162:185-98 pubmed
	Bland C, Kimberly P, Rand M. Notch-induced proteolysis and nuclear localization of the Delta ligand. J Biol Chem. 2003;278:13607-10 pubmed
	Mishra Gorur K, Rand M, Perez Villamil B, Artavanis Tsakonas S. Down-regulation of Delta by proteolytic processing. J Cell Biol. 2002;159:313-24 pubmed
	Panin V, Shao L, Lei L, Moloney D, Irvine K, Haltiwanger R. Notch ligands are substrates for protein O-fucosyltransferase-1 and Fringe. J Biol Chem. 2002;277:29945-52 pubmed
	Chihara T, Hayashi S. Control of tracheal tubulogenesis by Wingless signaling. Development. 2000;127:4433-42 pubmed
	Klueg K, Muskavitch M. Ligand-receptor interactions and trans-endocytosis of Delta, Serrate and Notch: members of the Notch signalling pathway in Drosophila. J Cell Sci. 1999;112 ( Pt 19):3289-97 pubmed
	Ikeya T, Hayashi S. Interplay of Notch and FGF signaling restricts cell fate and MAPK activation in the Drosophila trachea. Development. 1999;126:4455-63 pubmed
	Qi H, Rand M, Wu X, Sestan N, Wang W, Rakic P, et al. Processing of the notch ligand delta by the metalloprotease Kuzbanian. Science. 1999;283:91-4 pubmed
	Klueg K, Parody T, Muskavitch M. Complex proteolytic processing acts on Delta, a transmembrane ligand for Notch, during Drosophila development. Mol Biol Cell. 1998;9:1709-23 pubmed
	Sun X, Artavanis Tsakonas S. The intracellular deletions of Delta and Serrate define dominant negative forms of the Drosophila Notch ligands. Development. 1996;122:2465-74 pubmed
	Diederich R, Matsuno K, Hing H, Artavanis Tsakonas S. Cytosolic interaction between deltex and Notch ankyrin repeats implicates deltex in the Notch signaling pathway. Development. 1994;120:473-81 pubmed

product information

Internal ID :

427

Name :

C594.9B

Depositor Name :

Artavanis-Tsakonas, S.

Depositor Institution :

Harvard Medical School

Date Deposited :

5/2/00

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Delta, extracellular domain

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

9872749 8756291

Collections :

Cell adhesion,Cell markers,Cell signaling,Drosophila antigens,Neurodevelopment

Search Keywords :

Artavanis-Tsakonas, S., Delta, extracellular domain, Drosophila, MIgG1, Drosophila, Dl, CG3619, AB_528194, Monoclonal, Drosophila antigens/Cell markers/Neurodevelopment/Cell signaling/Cell adhesion, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 89 kDa; Apparent: full length and 65 kDa proteolytic extracellular fragment.

Gene :

Alternate Gene Name(s) :

CG3619

Uniprot ID :

P10041

Antibody Registry ID :

AB_528194

Synonyms (Alternate Clone Names) :

9B, MAb202, anti-Delta9B

Immunogen :

Drosophila Delta extracellular domain

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

Ag8

Epitope Mapped :

Yes

Epitope Location or Sequence :

EGF-like repeats 4 and/or 5

Epitope Map PubMed ID :

9658166

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Excellent

Immunohistochemistry Pubmed IDs :

16354601

Immunofluorescence Pubmed IDs :

9658166 10498681 11003842 19744473 23154412 17443769 16487968 8756291 18535782 10504334 23045391 15707586 27680705 29335529 29844017

Western Blot Pubmed IDs :

9872749 9658166 12403816 12591935 8162848 15707586

Immunoprecipitation Pubmed IDs :

9658166 15707586

Pubmed IDs :

12036964 15511641 20410139

Additional Information :

The extracellular fragment of Delta is a ligand for Notch

DSHB Growth Medium :

RPMI

References (legacy) :

Science 283, 91-94.; Development 126, 4455-4463.; Development 127, 4433-4442.; J. Biol. Chem. 277(33), 29945-29952.; Mech. Dev. 121, 1495-1507.; Eur. J. Med. Res. 10, 475-479.; Dev. Biol. 335, 228-236.; Mole. Biol. Cell 21, 2078-2086.; Development 140(1), 82-92.

more info or order :

Developmental Studies Hybridoma Bank product webpage