product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Coracle (common)
catalog :
C566.9
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
C566.9
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 12
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; fruit fly; 1:500; fig 4
  • western blot; fruit fly; 1:500; fig 4
Sollier K, Gaudé H, Chartier F, Laprise P. Rac1 controls epithelial tube length through the apical secretion and polarity pathways. Biol Open. 2015;5:49-54 pubmed publisher
  • immunohistochemistry; fruit fly; 1:25; loading ...; tbl 3
Flores Benitez D, Knust E. Crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in Drosophila. elife. 2015;4: pubmed publisher
  • immunohistochemistry; fruit fly; 1:500
Fairchild M, Smendziuk C, Tanentzapf G. A somatic permeability barrier around the germline is essential for Drosophila spermatogenesis. Development. 2015;142:268-81 pubmed publisher
Jiang N, Rasmussen J, Clanton J, Rosenberg M, Luedke K, Cronan M, et al. A conserved morphogenetic mechanism for epidermal ensheathment of nociceptive sensory neurites. elife. 2019;8: pubmed publisher
Loganathan R, Lee J, Wells M, GREVENGOED E, Slattery M, Andrew D. Ribbon regulates morphogenesis of the Drosophila embryonic salivary gland through transcriptional activation and repression. Dev Biol. 2016;409:234-250 pubmed publisher
Song Y, Eng M, Ghabrial A. Focal defects in single-celled tubes mutant for Cerebral cavernous malformation 3, GCKIII, or NSF2. Dev Cell. 2013;25:507-19 pubmed publisher
Gómez J, Wang Y, Riechmann V. Tao controls epithelial morphogenesis by promoting Fasciclin 2 endocytosis. J Cell Biol. 2012;199:1131-43 pubmed publisher
Ile K, Tripathy R, Goldfinger V, Renault A. Wunen, a Drosophila lipid phosphate phosphatase, is required for septate junction-mediated barrier function. Development. 2012;139:2535-46 pubmed publisher
Gibbens Y, Warren J, Gilbert L, O Connor M. Neuroendocrine regulation of Drosophila metamorphosis requires TGFbeta/Activin signaling. Development. 2011;138:2693-703 pubmed publisher
Tanentzapf G, Tepass U. Interactions between the crumbs, lethal giant larvae and bazooka pathways in epithelial polarization. Nat Cell Biol. 2003;5:46-52 pubmed
Fernandez R, Takahashi F, Liu Z, Steward R, Stein D, Stanley E. The Drosophila shark tyrosine kinase is required for embryonic dorsal closure. Genes Dev. 2000;14:604-14 pubmed
Fehon R, Dawson I, Artavanis Tsakonas S. A Drosophila homologue of membrane-skeleton protein 4.1 is associated with septate junctions and is encoded by the coracle gene. Development. 1994;120:545-57 pubmed
product information
Internal ID :
3037
Name :
C566.9
Depositor Name :
Fehon, R.
Depositor Institution :
University of Chicago
Date Deposited :
8/25/07
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Coracle (common)
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
8162854
Collections :
Cell signaling,Cytoskeleton,Drosophila antigens,Skeletal muscle
Search Keywords :
Fehon, R., Coracle (common), Drosophila, MIgG1, kappa light chain , Drosophila, cora, CG11949, Cor, Coracle, D4.1, AB_1161642, monoclonal, Cell signaling/Cytoskeletal elements/Drosophila antigens/Skeletal muscle, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation
Antigen Molecular Weight :
Predicted: multiple isoforms from 78 to 184 kDa. Apparent: 200 kDa.
Gene :
cora
Alternate Gene Name(s) :
CG11949, Cor, Coracle, D4.1
Uniprot ID :
Q9V8R9
Antibody Registry ID :
AB_1161642
Immunogen :
Recombinant Coracle FERM domain (aa 72-397)/GST fusion protein
Immunogen Sequence :
Partial protein
Clonality :
Monoclonal
Epitope Mapped :
Yes
Epitope Location or Sequence :
aa 72-397 of isoform 1 (Uniprot ID: Q9V8R9-1).
Epitope Map PubMed ID :
8162854
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
8162854 23266957 10716948
Immunofluorescence Pubmed IDs :
26477561 23266957 21613324 26700724 25503408 22675212 23763949 12510193
Western Blot Pubmed IDs :
8162854 26700724
Additional Information :
This antibody will recognize all coracle isoforms except maybe isoform 5 (Uniprot ID: Q9V8R9-5). This antigen is also known as protein 4.1 homolog and is a component of septate junctions.
DSHB Growth Medium :
Iscove's
References (legacy) :
Development 120, 545-557.; J. Cell Biol. 140, 1463-1473.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US