product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Coracle (common)
catalog :
C566.9
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
C566.9
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
citations: 12
Published Application/Species/Sample/Dilution | Reference |
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Tanentzapf G, Tepass U. Interactions between the crumbs, lethal giant larvae and bazooka pathways in epithelial polarization. Nat Cell Biol. 2003;5:46-52 pubmed
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Fernandez R, Takahashi F, Liu Z, Steward R, Stein D, Stanley E. The Drosophila shark tyrosine kinase is required for embryonic dorsal closure. Genes Dev. 2000;14:604-14 pubmed
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Fehon R, Dawson I, Artavanis Tsakonas S. A Drosophila homologue of membrane-skeleton protein 4.1 is associated with septate junctions and is encoded by the coracle gene. Development. 1994;120:545-57 pubmed
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product information
Internal ID :
3037
Name :
C566.9
Depositor Name :
Fehon, R.
Depositor Institution :
University of Chicago
Date Deposited :
8/25/07
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Coracle (common)
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
8162854
Collections :
Cell signaling,Cytoskeleton,Drosophila antigens,Skeletal muscle
Search Keywords :
Fehon, R., Coracle (common), Drosophila, MIgG1, kappa light chain , Drosophila, cora, CG11949, Cor, Coracle, D4.1, AB_1161642, monoclonal, Cell signaling/Cytoskeletal elements/Drosophila antigens/Skeletal muscle, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation
Antigen Molecular Weight :
Predicted: multiple isoforms from 78 to 184 kDa. Apparent: 200 kDa.
Gene :
cora
Alternate Gene Name(s) :
CG11949, Cor, Coracle, D4.1
Uniprot ID :
Q9V8R9
Antibody Registry ID :
AB_1161642
Immunogen :
Recombinant Coracle FERM domain (aa 72-397)/GST fusion protein
Immunogen Sequence :
Partial protein
Clonality :
Monoclonal
Epitope Mapped :
Yes
Epitope Location or Sequence :
aa 72-397 of isoform 1 (Uniprot ID: Q9V8R9-1).
Epitope Map PubMed ID :
8162854
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
8162854 23266957 10716948
Immunofluorescence Pubmed IDs :
26477561 23266957 21613324 26700724 25503408 22675212 23763949 12510193
Western Blot Pubmed IDs :
8162854 26700724
Additional Information :
This antibody will recognize all coracle isoforms except maybe isoform 5 (Uniprot ID: Q9V8R9-5). This antigen is also known as protein 4.1 homolog and is a component of septate junctions.
DSHB Growth Medium :
Iscove's
References (legacy) :
Development 120, 545-557.; J. Cell Biol. 140, 1463-1473.
more info or order :
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Developmental Studies Hybridoma Bank
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