slit protein, C-terminal | Developmental Studies Hybridoma Bank C555.6D product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

slit protein, C-terminal

catalog :

C555.6D

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C555.6D

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; 1:30; loading ...; fig 4A	Raza Q, Vanderploeg J, Jacobs J. Matrix Metalloproteinases are required for membrane motility and lumenogenesis during Drosophila heart development. PLoS ONE. 2017;12:e0171905 pubmed publisher
immunocytochemistry; fruit fly; 1:50; fig 1	Reichert M, Brown H, Evans T. In vivo functional analysis of Drosophila Robo1 immunoglobulin-like domains. Neural Dev. 2016;11:15 pubmed publisher
immunocytochemistry; fruit fly; 1:50	Brown H, Reichert M, Evans T. Slit Binding via the Ig1 Domain Is Essential for Midline Repulsion by Drosophila Robo1 but Dispensable for Receptor Expression, Localization, and Regulation in Vivo. G3 (Bethesda). 2015;5:2429-39 pubmed publisher
immunocytochemistry; fruit fly; 1:100; fig 5	Chance R, Bashaw G. Slit-Dependent Endocytic Trafficking of the Robo Receptor Is Required for Son of Sevenless Recruitment and Midline Axon Repulsion. PLoS Genet. 2015;11:e1005402 pubmed publisher
immunohistochemistry; fruit fly; 1:50; fig 4	Evans T, Santiago C, Arbeille E, Bashaw G. Robo2 acts in trans to inhibit Slit-Robo1 repulsion in pre-crossing commissural axons. elife. 2015;4:e08407 pubmed publisher
	Sallé J, Gervais L, Boumard B, Stefanutti M, Siudeja K, Bardin A. Intrinsic regulation of enteroendocrine fate by Numb. EMBO J. 2017;36:1928-1945 pubmed publisher
	Drechsler M, Schmidt A, Meyer H, Paululat A. The conserved ADAMTS-like protein lonely heart mediates matrix formation and cardiac tissue integrity. PLoS Genet. 2013;9:e1003616 pubmed publisher
	Shen S, Aleksic J, Russell S. Identifying targets of the Sox domain protein Dichaete in the Drosophila CNS via targeted expression of dominant negative proteins. BMC Dev Biol. 2013;13:1 pubmed publisher
	Slováková J, Carmena A. Canoe functions at the CNS midline glia in a complex with Shotgun and Wrapper-Nrx-IV during neuron-glia interactions. Development. 2011;138:1563-71 pubmed publisher
	Albrecht S, Altenhein B, Paululat A. The transmembrane receptor Uncoordinated5 (Unc5) is essential for heart lumen formation in Drosophila melanogaster. Dev Biol. 2011;350:89-100 pubmed publisher
	Banerjee S, Blauth K, Peters K, Rogers S, Fanning A, Bhat M. Drosophila neurexin IV interacts with Roundabout and is required for repulsive midline axon guidance. J Neurosci. 2010;30:5653-67 pubmed publisher
	Evans I, Hu N, Skaer H, Wood W. Interdependence of macrophage migration and ventral nerve cord development in Drosophila embryos. Development. 2010;137:1625-33 pubmed publisher
	Bahri S, Choy J, Manser E, Lim L, Yang X. The Drosophila homologue of Arf-GAP GIT1, dGIT, is required for proper muscle morphogenesis and guidance during embryogenesis. Dev Biol. 2009;325:15-23 pubmed publisher
	Furrer M, Vasenkova I, Kamiyama D, Rosado Y, Chiba A. Slit and Robo control the development of dendrites in Drosophila CNS. Development. 2007;134:3795-804 pubmed
	Bhat K, Gaziova I, Krishnan S. Regulation of axon guidance by slit and netrin signaling in the Drosophila ventral nerve cord. Genetics. 2007;176:2235-46 pubmed
	Lee S, Nahm M, Lee M, Kwon M, Kim E, Zadeh A, et al. The F-actin-microtubule crosslinker Shot is a platform for Krasavietz-mediated translational regulation of midline axon repulsion. Development. 2007;134:1767-77 pubmed
	Hiramoto M, Hiromi Y. ROBO directs axon crossing of segmental boundaries by suppressing responsiveness to relocalized Netrin. Nat Neurosci. 2006;9:58-66 pubmed
	Tayler T, Robichaux M, Garrity P. Compartmentalization of visual centers in the Drosophila brain requires Slit and Robo proteins. Development. 2004;131:5935-45 pubmed
	Rothberg J, Jacobs J, Goodman C, Artavanis Tsakonas S. slit: an extracellular protein necessary for development of midline glia and commissural axon pathways contains both EGF and LRR domains. Genes Dev. 1990;4:2169-87 pubmed

product information

Internal ID :

421

Name :

C555.6D

Depositor Name :

Artavanis-Tsakonas, S.

Depositor Institution :

Harvard Medical School

Date Deposited :

5/2/00

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

slit protein, C-terminal

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG2b, kappa light chain

Isotype for catalog (legacy) :

IgG2b, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

2176636

Depositor Notes (Special Instructions) :

This antibody was generated against the C-terminal region of the protein slit

Collections :

Cardiac development,Cell signaling,Drosophila antigens,Extracellular matrix proteins,Neurodevelopment

Search Keywords :

Artavanis-Tsakonas, S., slit protein, C-terminal, Drosophila, MIgG2b, kappa light chain, Drosophila, sli, CG43758, AB_528470, Monoclonal, Drosophila antigens/Neurodevelopment/Cell signaling/Extracellular matrix proteins/Cardiac development, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted; 169 kDa; Apparent 190 kDa and smaller proteolytic fragments containing the C-terminal region of the Slit protein

Gene :

sli

Alternate Gene Name(s) :

CG43758

Uniprot ID :

P24014

Antibody Registry ID :

AB_528470

Synonyms (Alternate Clone Names) :

anti-slit, anti-Slit-C

Immunogen :

Recombinant fusion protein containing the C-terminal region of slit, aa 1311-1480 (isoformorm A; Uniprot ID P24014-2)

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

Ag8

Epitope Mapped :

Yes

Epitope Location or Sequence :

C-terminal portion, aa 1311-1480 [see depositor notes]

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Excellent

Immunohistochemistry Pubmed IDs :

2176636 17565966

Immunofluorescence Pubmed IDs :

2176636 20392742 21094637 20410118 18996366 21389054 16341212 17409115 23874219 15525663 17933790

Western Blot Pubmed IDs :

2176636 20410118 17933790 17565966 26335920

Immunoprecipitation Pubmed IDs :

2176636 20410118

DSHB Growth Medium :

RPMI

References (legacy) :

Genes Dev. 4, 2169-2187.; J. Innate Immun. 1(4), 322-334.; Development 137(10), 1625-1633.; Dev. Biol. 350, 89-100.

more info or order :

Developmental Studies Hybridoma Bank product webpage