Notch, extracellular domain, EGF repeats #12-20 | Developmental Studies Hybridoma Bank C458.2H product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Notch, extracellular domain, EGF repeats #12-20

catalog :

C458.2H

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C458.2H

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 27

Reference
Fic W, Faria C, St Johnston D. IMP regulates Kuzbanian to control the timing of Notch signalling in Drosophila follicle cells. Development. 2019;146: pubmed publisher
Vissers J, Froldi F, Schröder J, Papenfuss A, Cheng L, Harvey K. The Scalloped and Nerfin-1 Transcription Factors Cooperate to Maintain Neuronal Cell Fate. Cell Rep. 2018;25:1561-1576.e7 pubmed publisher
Li B, Wong C, Gao S, Zhang R, Sun R, Li Y, et al. The retromer complex safeguards against neural progenitor-derived tumorigenesis by regulating Notch receptor trafficking. elife. 2018;7: pubmed publisher
Court H, Ahearn I, Amoyel M, Bach E, Philips M. Regulation of NOTCH signaling by RAB7 and RAB8 requires carboxyl methylation by ICMT. J Cell Biol. 2017;216:4165-4182 pubmed publisher
HegedÅ±s K, TakÃ¡ts S, Boda A, Jipa A, Nagy P, Varga K, et al. The Ccz1-Mon1-Rab7 module and Rab5 control distinct steps of autophagy. Mol Biol Cell. 2016;27:3132-3142 pubmed
Troost T, Schneider M, Klein T. A re-examination of the selection of the sensory organ precursor of the bristle sensilla of Drosophila melanogaster. PLoS Genet. 2015;11:e1004911 pubmed publisher
Morelli E, Ginefra P, Mastrodonato V, Beznoussenko G, Rusten T, Bilder D, et al. Multiple functions of the SNARE protein Snap29 in autophagy, endocytic, and exocytic trafficking during epithelial formation in Drosophila. Autophagy. 2014;10:2251-68 pubmed publisher
Levy P, Larsen C. Odd-skipped labels a group of distinct neurons associated with the mushroom body and optic lobe in the adult Drosophila brain. J Comp Neurol. 2013;521:3716-40 pubmed publisher
van de Hoef D, Bonner J, Boulianne G. FKBP14 is an essential gene that regulates Presenilin protein levels and Notch signaling in Drosophila. Development. 2013;140:810-9 pubmed publisher
Dornier E, Coumailleau F, Ottavi J, Moretti J, Boucheix C, Mauduit P, et al. TspanC8 tetraspanins regulate ADAM10/Kuzbanian trafficking and promote Notch activation in flies and mammals. J Cell Biol. 2012;199:481-96 pubmed publisher
Perdigoto C, Schweisguth F, Bardin A. Distinct levels of Notch activity for commitment and terminal differentiation of stem cells in the adult fly intestine. Development. 2011;138:4585-95 pubmed publisher
Djiane A, Shimizu H, Wilkin M, Mazleyrat S, Jennings M, Avis J, et al. Su(dx) E3 ubiquitin ligase-dependent and -independent functions of polychaetoid, the Drosophila ZO-1 homologue. J Cell Biol. 2011;192:189-200 pubmed publisher
Benhra N, Vignaux F, Dussert A, Schweisguth F, Le Borgne R. Neuralized promotes basal to apical transcytosis of delta in epithelial cells. Mol Biol Cell. 2010;21:2078-86 pubmed publisher
Fiuza U, Klein T, Martinez Arias A, Hayward P. Mechanisms of ligand-mediated inhibition in Notch signaling activity in Drosophila. Dev Dyn. 2010;239:798-805 pubmed publisher
Matsuura A, Ito M, Sakaidani Y, Kondo T, Murakami K, Furukawa K, et al. O-linked N-acetylglucosamine is present on the extracellular domain of notch receptors. J Biol Chem. 2008;283:35486-95 pubmed publisher
Okajima T, Reddy B, Matsuda T, Irvine K. Contributions of chaperone and glycosyltransferase activities of O-fucosyltransferase 1 to Notch signaling. BMC Biol. 2008;6:1 pubmed publisher
Song X, Call G, Kirilly D, Xie T. Notch signaling controls germline stem cell niche formation in the Drosophila ovary. Development. 2007;134:1071-80 pubmed
Baonza A, Freeman M. Control of cell proliferation in the Drosophila eye by Notch signaling. Dev Cell. 2005;8:529-39 pubmed
Ahimou F, Mok L, Bardot B, Wesley C. The adhesion force of Notch with Delta and the rate of Notch signaling. J Cell Biol. 2004;167:1217-29 pubmed
Kamimura K, Rhodes J, Ueda R, McNeely M, Shukla D, Kimata K, et al. Regulation of Notch signaling by Drosophila heparan sulfate 3-O sulfotransferase. J Cell Biol. 2004;166:1069-79 pubmed
Okajima T, Irvine K. Regulation of notch signaling by o-linked fucose. Cell. 2002;111:893-904 pubmed
Goto S, Taniguchi M, Muraoka M, Toyoda H, Sado Y, Kawakita M, et al. UDP-sugar transporter implicated in glycosylation and processing of Notch. Nat Cell Biol. 2001;3:816-22 pubmed
Parks A, Klueg K, Stout J, Muskavitch M. Ligand endocytosis drives receptor dissociation and activation in the Notch pathway. Development. 2000;127:1373-85 pubmed
Rauskolb C, Correia T, Irvine K. Fringe-dependent separation of dorsal and ventral cells in the Drosophila wing. Nature. 1999;401:476-80 pubmed
Ye Y, Fortini M. Apoptotic activities of wild-type and Alzheimer's disease-related mutant presenilins in Drosophila melanogaster. J Cell Biol. 1999;146:1351-64 pubmed
Ye Y, Lukinova N, Fortini M. Neurogenic phenotypes and altered Notch processing in Drosophila Presenilin mutants. Nature. 1999;398:525-9 pubmed
Diederich R, Matsuno K, Hing H, Artavanis Tsakonas S. Cytosolic interaction between deltex and Notch ankyrin repeats implicates deltex in the Notch signaling pathway. Development. 1994;120:473-81 pubmed

product information

Internal ID :

415

Name :

C458.2H

Depositor Name :

Artavanis-Tsakonas, S.

Depositor Institution :

Harvard Medical School

Date Deposited :

5/2/00

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Notch, extracellular domain, EGF repeats #12-20

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

8162848

Depositor Notes (Special Instructions) :

This antibody was generated against the extracellular domain of Notch containing the EGF repeats #12-20

Collections :

Cardiac development,Cell adhesion,Cell markers,Cell signaling,Drosophila antigens,Neurodevelopment,Skeletal muscle,Transcription factors

Search Keywords :

Artavanis-Tsakonas, S., Notch, extracellular domain, EGF repeats #12-20, Drosophila, MIgG1, kappa light chain, Drosophila, N, CG3936, Notch, NECD, NICD, AB_528408, Monoclonal, Drosophila antigens/Cell markers/Transcription factors/Neurodevelopment/Cell signaling/Cardiac development/Cell adhesion/Skeletal muscle, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 289 kDa; Apparent: 300 kDa and lower MW N-terminal fragment, resulting from proteolysis.

Gene :

Alternate Gene Name(s) :

CG3936, Notch, NECD, NICD

Uniprot ID :

P07207

Antibody Registry ID :

AB_528408

Immunogen :

Recombinant fusion protein containing the EGF repeats #12-20

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

Ag8

Epitope Mapped :

Yes

Epitope Location or Sequence :

extracellular domain, EGF repeats #12-20 [see depositor notes]

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Excellent

Immunohistochemistry Pubmed IDs :

10704384 10519550

Immunofluorescence Pubmed IDs :

8162848 11533661 12526814 10206647 23318643 18194540 10704384 15809035 20063416 21965616 10491396 17287246

Western Blot Pubmed IDs :

18948267 20063416

Immunoprecipitation Pubmed IDs :

18948267

Additional Information :

The Neurogenic locus Notch protein functions as a transmembrane receptor for membrane-bound ligands Delta and Serrate to regulate neurodevelopment. Upon ligand activation, the intracellular domain (NICD) is released from the cell membrane, and forms a transcriptional activator complex with the Suppressor of hairless protein.

DSHB Growth Medium :

RPMI

References (legacy) :

Development 120, 473-481.; Nature Cell Biol. 3(9), 816-822.; Cell 111, 893-904.; Mole. Biol. Cell 21, 2078-2086.; Development 140, 810-819.

more info or order :

Developmental Studies Hybridoma Bank product webpage