Heterochromatin Protein 1 | Developmental Studies Hybridoma Bank C1A9 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Heterochromatin Protein 1

catalog :

C1A9

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C1A9

reactivity :

fruit fly

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, proximity ligation assay

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 43

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; fruit fly; 1:500; fig 5-s1	Swenson J, Colmenares S, Strom A, Costes S, Karpen G. The composition and organization of Drosophila heterochromatin are heterogeneous and dynamic. elife. 2016;5: pubmed publisher
proximity ligation assay; fruit fly; fig 4 immunoprecipitation; fruit fly; fig 2 immunocytochemistry; fruit fly; fig 1 western blot; fruit fly; fig 6	Eberle A, Jordán Pla A, Gañez Zapater A, Hessle V, Silberberg G, von Euler A, et al. An Interaction between RRP6 and SU(VAR)3-9 Targets RRP6 to Heterochromatin and Contributes to Heterochromatin Maintenance in Drosophila melanogaster. PLoS Genet. 2015;11:e1005523 pubmed publisher
immunocytochemistry; fruit fly; 1:10; fig 7	Cenci G, Ciapponi L, Marzullo M, Raffa G, Morciano P, Raimondo D, et al. The Analysis of Pendolino (peo) Mutants Reveals Differences in the Fusigenic Potential among Drosophila Telomeres. PLoS Genet. 2015;11:e1005260 pubmed publisher
	Brueckner L, van Arensbergen J, Akhtar W, Pagie L, van Steensel B. High-throughput assessment of context-dependent effects of chromatin proteins. Epigenetics Chromatin. 2016;9:43 pubmed
	Hong S, Choi K. Antagonistic roles of Drosophila Tctp and Brahma in chromatin remodelling and stabilizing repeated sequences. Nat Commun. 2016;7:12988 pubmed publisher
	Wang C, Sun H, Zou R, Zhou T, Wang S, Sun S, et al. MDC1 functionally identified as an androgen receptor co-activator participates in suppression of prostate cancer. Nucleic Acids Res. 2015;43:4893-908 pubmed publisher
	López Falcón B, Meyer Nava S, Hernández Rodríguez B, Campos A, Montero D, Rudiño E, et al. Characterization of the Drosophila group ortholog to the amino-terminus of the alpha-thalassemia and mental retardation X-Linked (ATRX) vertebrate protein. PLoS ONE. 2014;9:e113182 pubmed publisher
	Xu N, Emelyanov A, Fyodorov D, Skoultchi A. Drosophila linker histone H1 coordinates STAT-dependent organization of heterochromatin and suppresses tumorigenesis caused by hyperactive JAK-STAT signaling. Epigenetics Chromatin. 2014;7:16 pubmed publisher
	Marr S, Lis J, Treisman J, Marr M. The metazoan-specific mediator subunit 26 (Med26) is essential for viability and is found at both active genes and pericentric heterochromatin in Drosophila melanogaster. Mol Cell Biol. 2014;34:2710-20 pubmed
	Basquin D, Spierer A, Begeot F, Koryakov D, Todeschini A, Ronsseray S, et al. The Drosophila Su(var)3-7 gene is required for oogenesis and female fertility, genetically interacts with piwi and aubergine, but impacts only weakly transposon silencing. PLoS ONE. 2014;9:e96802 pubmed publisher
	Dufourt J, Dennis C, Boivin A, Gueguen N, Théron E, Goriaux C, et al. Spatio-temporal requirements for transposable element piRNA-mediated silencing during Drosophila oogenesis. Nucleic Acids Res. 2014;42:2512-24 pubmed publisher
	Jiang N, Du G, Tobias E, Wood J, Whitaker R, Neretti N, et al. Dietary and genetic effects on age-related loss of gene silencing reveal epigenetic plasticity of chromatin repression during aging. Aging (Albany NY). 2013;5:813-24 pubmed
	Schoborg T, Rickels R, Barrios J, Labrador M. Chromatin insulator bodies are nuclear structures that form in response to osmotic stress and cell death. J Cell Biol. 2013;202:261-76 pubmed publisher
	Herzog S, Nagarkar Jaiswal S, Urban E, Riemer A, Fischer S, Heidmann S. Functional dissection of the Drosophila melanogaster condensin subunit Cap-G reveals its exclusive association with condensin I. PLoS Genet. 2013;9:e1003463 pubmed publisher
	Bugga L, McDaniel I, Engie L, Armstrong J. The Drosophila melanogaster CHD1 chromatin remodeling factor modulates global chromosome structure and counteracts HP1a and H3K9me2. PLoS ONE. 2013;8:e59496 pubmed publisher
	Sienski G, Donertas D, Brennecke J. Transcriptional silencing of transposons by Piwi and maelstrom and its impact on chromatin state and gene expression. Cell. 2012;151:964-80 pubmed publisher
	Maheshwari S, Barbash D. Cis-by-Trans regulatory divergence causes the asymmetric lethal effects of an ancestral hybrid incompatibility gene. PLoS Genet. 2012;8:e1002597 pubmed publisher
	Larson K, Yan S, Tsurumi A, Liu J, Zhou J, Gaur K, et al. Heterochromatin formation promotes longevity and represses ribosomal RNA synthesis. PLoS Genet. 2012;8:e1002473 pubmed publisher
	Dronamraju R, Mason J. MU2 and HP1a regulate the recognition of double strand breaks in Drosophila melanogaster. PLoS ONE. 2011;6:e25439 pubmed publisher
	Dufourt J, Brasset E, Desset S, Pouchin P, Vaury C. Polycomb group-dependent, heterochromatin protein 1-independent, chromatin structures silence retrotransposons in somatic tissues outside ovaries. DNA Res. 2011;18:451-61 pubmed publisher
	Wood J, Hillenmeyer S, Lawrence C, Chang C, Hosier S, Lightfoot W, et al. Chromatin remodeling in the aging genome of Drosophila. Aging Cell. 2010;9:971-8 pubmed publisher
	Sharakhova M, George P, Brusentsova I, Leman S, Bailey J, Smith C, et al. Genome mapping and characterization of the Anopheles gambiae heterochromatin. BMC Genomics. 2010;11:459 pubmed publisher
	Moshkovich N, Lei E. HP1 recruitment in the absence of argonaute proteins in Drosophila. PLoS Genet. 2010;6:e1000880 pubmed publisher
	Salzler H, Davidson J, Montgomery N, Duronio R. Loss of the histone pre-mRNA processing factor stem-loop binding protein in Drosophila causes genomic instability and impaired cellular proliferation. PLoS ONE. 2009;4:e8168 pubmed publisher
	Piacentini L, Fanti L, Negri R, Del Vescovo V, Fatica A, Altieri F, et al. Heterochromatin protein 1 (HP1a) positively regulates euchromatic gene expression through RNA transcript association and interaction with hnRNPs in Drosophila. PLoS Genet. 2009;5:e1000670 pubmed publisher
	Koch C, Honemann Capito M, Egger Adam D, Wodarz A. Windei, the Drosophila homolog of mAM/MCAF1, is an essential cofactor of the H3K9 methyl transferase dSETDB1/Eggless in germ line development. PLoS Genet. 2009;5:e1000644 pubmed publisher
	Gracheva E, Dus M, Elgin S. Drosophila RISC component VIG and its homolog Vig2 impact heterochromatin formation. PLoS ONE. 2009;4:e6182 pubmed publisher
	Cakouros D, Mills K, Denton D, Paterson A, Daish T, Kumar S. dLKR/SDH regulates hormone-mediated histone arginine methylation and transcription of cell death genes. J Cell Biol. 2008;182:481-95 pubmed publisher
	Shi S, Larson K, Guo D, Lim S, Dutta P, Yan S, et al. Drosophila STAT is required for directly maintaining HP1 localization and heterochromatin stability. Nat Cell Biol. 2008;10:489-96 pubmed publisher
	Smith E, Lee M, Winter B, Droz N, Eissenberg J, Shiekhattar R, et al. Drosophila UTX is a histone H3 Lys27 demethylase that colocalizes with the elongating form of RNA polymerase II. Mol Cell Biol. 2008;28:1041-6 pubmed
	Bongiorni S, Pasqualini B, Taranta M, Singh P, Prantera G. Epigenetic regulation of facultative heterochromatinisation in Planococcus citri via the Me(3)K9H3-HP1-Me(3)K20H4 pathway. J Cell Sci. 2007;120:1072-80 pubmed
	Shi S, Calhoun H, Xia F, Li J, Le L, Li W. JAK signaling globally counteracts heterochromatic gene silencing. Nat Genet. 2006;38:1071-6 pubmed
	Liu L, Ni J, Shi Y, Oakeley E, Sun F. Sex-specific role of Drosophila melanogaster HP1 in regulating chromatin structure and gene transcription. Nat Genet. 2005;37:1361-6 pubmed
	De Lucia F, Ni J, Vaillant C, Sun F. HP1 modulates the transcription of cell-cycle regulators in Drosophila melanogaster. Nucleic Acids Res. 2005;33:2852-8 pubmed
	Cryderman D, Grade S, Li Y, Fanti L, Pimpinelli S, Wallrath L. Role of Drosophila HP1 in euchromatic gene expression. Dev Dyn. 2005;232:767-74 pubmed
	Perrini B, Piacentini L, Fanti L, Altieri F, Chichiarelli S, Berloco M, et al. HP1 controls telomere capping, telomere elongation, and telomere silencing by two different mechanisms in Drosophila. Mol Cell. 2004;15:467-76 pubmed
	Piacentini L, Fanti L, Berloco M, Perrini B, Pimpinelli S. Heterochromatin protein 1 (HP1) is associated with induced gene expression in Drosophila euchromatin. J Cell Biol. 2003;161:707-14 pubmed
	Fanti L, Berloco M, Piacentini L, Pimpinelli S. Chromosomal distribution of heterochromatin protein 1 (HP1) in Drosophila: a cytological map of euchromatic HP1 binding sites. Genetica. 2003;117:135-47 pubmed
	Cenci G, Siriaco G, Raffa G, Kellum R, Gatti M. The Drosophila HOAP protein is required for telomere capping. Nat Cell Biol. 2003;5:82-4 pubmed
	Fanti L, Giovinazzo G, Berloco M, Pimpinelli S. The heterochromatin protein 1 prevents telomere fusions in Drosophila. Mol Cell. 1998;2:527-38 pubmed
	Cléard F, Delattre M, Spierer P. SU(VAR)3-7, a Drosophila heterochromatin-associated protein and companion of HP1 in the genomic silencing of position-effect variegation. EMBO J. 1997;16:5280-8 pubmed
	Platero J, Hartnett T, Eissenberg J. Functional analysis of the chromo domain of HP1. EMBO J. 1995;14:3977-86 pubmed
	James T, Elgin S. Identification of a nonhistone chromosomal protein associated with heterochromatin in Drosophila melanogaster and its gene. Mol Cell Biol. 1986;6:3862-72 pubmed

product information

Internal ID :

909

Name :

C1A9

Depositor Name :

Wallrath, L.L.

Depositor Institution :

University of Iowa

Date Deposited :

1/4/02

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Heterochromatin Protein 1

Antigen Species :

Drosophila melanogaster

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila,Planococcus citri

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

3099166

Depositor Notes (Special Instructions) :

detects HP1, a structural component of heterochromatin, on fixed squashed and whole mount Drosophila melanogaster salivary gland polytene chromosomes

Collections :

Drosophila antigens,Nucleus

Search Keywords :

Wallrath, L.L., Heterochromatin Protein 1, Drosophila melanogaster, MIgG1, kappa light chain, Drosophila/Planococcus citri, Su(var)205, HP1, CG8409, AB_528276, Monoclonal, Drosophila antigens/Nucleus, Immunofluorescence/Western Blot/Immunoprecipitation/Chromatin Immunoprecipitation

Antigen Molecular Weight :

Predicted: 23 kDa; Apparent 24 kDa

Gene :

Su(var)205

Alternate Gene Name(s) :

HP1, CG8409

Uniprot ID :

P05205

Antibody Registry ID :

AB_528276

Immunogen :

Nuclear proteins extracted in 2.0 M potassium thiocyanate

Immunogen Sequence :

Total protein

Clonality :

Monoclonal

Myeloma Strain :

Sp2/0 Ag14

Epitope Mapped :

Recommended Applications :

Chromatin Immunoprecipitation,Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

27687497

Immunofluorescence Pubmed IDs :

3099166 9311988 9844626 12723693 16258543 7664737 16892059 12756231 15304225 15704177 17327272 18344984 24820420 19584931 20961390 22291607 18039863 30389853

Western Blot Pubmed IDs :

3099166 27687497 9311988 12510197 16258543 16892059 12756231 15304225 18344984 15905474 24820420 22291607

Immunoprecipitation Pubmed IDs :

19798443 19584931

Chromatin Immunoprecipitation Pubmed IDs :

12756231 18344984 20961390 22291607

DSHB Growth Medium :

RPMI

References (legacy) :

Mol. Cell. Biol. 6, 3862-3872.; Proc. Natl. Acad. Sci. USA 87, 9923-9927.

more info or order :

Developmental Studies Hybridoma Bank product webpage