Notch, intracellular domain | Developmental Studies Hybridoma Bank C17.9C6 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Notch, intracellular domain

catalog :

C17.9C6

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C17.9C6

reactivity :

Drosophila melanogaster

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 53

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; Drosophila melanogaster; 1:50; loading ...; fig 4d	Jörg D, Caygill E, Hakes A, Contreras E, Brand A, Simons B. The proneural wave in the Drosophila optic lobe is driven by an excitable reaction-diffusion mechanism. elife. 2019;8: pubmed publisher
immunohistochemistry; Drosophila melanogaster; 1:1000; fig 1e	Loubiere V, Delest A, Thomas A, Bonev B, Schuettengruber B, Sati S, et al. Coordinate redeployment of PRC1 proteins suppresses tumor formation during Drosophila development. Nat Genet. 2016;48:1436-1442 pubmed publisher
western blot; Drosophila melanogaster; 1:500; fig 2d	Erdmann I, Marter K, Kobler O, Niehues S, Abele J, MÃ¼ller A, et al. Cell-selective labelling of proteomes in Drosophila melanogaster. Nat Commun. 2015;6:7521 pubmed publisher
	Zhao S, Wu C, Gao Z, Li X, Guo Z, Wang Z. Notch signaling governs the expression of glypican Dally to define the stem cell niche. Biol Open. 2020;9: pubmed publisher
	Paddibhatla I, Gautam D, Mishra R. SETDB1 modulates the differentiation of both the crystal cells and the lamellocytes in Drosophila. Dev Biol. 2019;456:74-85 pubmed publisher
	Fic W, Faria C, St Johnston D. IMP regulates Kuzbanian to control the timing of Notch signalling in Drosophila follicle cells. Development. 2019;146: pubmed publisher
	Li Y, Liu T, Zhang J. The ATPase TER94 regulates Notch signaling during Drosophila wing development. Biol Open. 2019;8: pubmed publisher
	Cara F, Bülow M, Simmonds A, Rachubinski R. Dysfunctional peroxisomes compromise gut structure and host defense by increased cell death and Tor-dependent autophagy. Mol Biol Cell. 2018;29:2766-2783 pubmed publisher
	Wissel S, Harzer H, Bonnay F, Burkard T, Neumüller R, Knoblich J. Time-resolved transcriptomics in neural stem cells identifies a v-ATPase/Notch regulatory loop. J Cell Biol. 2018;217:3285-3300 pubmed publisher
	Del Bel L, Griffiths N, Wilk R, Wei H, Blagoveshchenskaya A, Burgess J, et al. The phosphoinositide phosphatase Sac1 regulates cell shape and microtubule stability in the developing Drosophila eye. Development. 2018;145: pubmed publisher
	Gomez Lamarca M, Falo Sanjuan J, Stojnic R, Abdul Rehman S, Muresan L, Jones M, et al. Activation of the Notch Signaling Pathway In Vivo Elicits Changes in CSL Nuclear Dynamics. Dev Cell. 2018;44:611-623.e7 pubmed publisher
	Wang Z, Lyu J, Wang F, Miao C, Nan Z, Zhang J, et al. The histone deacetylase HDAC1 positively regulates Notch signaling during Drosophila wing development. Biol Open. 2018;7: pubmed publisher
	Ando T, Fujiwara H, Kojima T. The pivotal role of aristaless in development and evolution of diverse antennal morphologies in moths and butterflies. BMC Evol Biol. 2018;18:8 pubmed publisher
	Kannan R, Cox E, Wang L, Kuzina I, Gu Q, Giniger E. Tyrosine phosphorylation and proteolytic cleavage of Notch are required for non-canonical Notch/Abl signaling in Drosophila axon guidance. Development. 2018;145: pubmed publisher
	Shukla J, Deshpande G, Shashidhara L. Ataxin 2-binding protein 1 is a context-specific positive regulator of Notch signaling during neurogenesis in Drosophila melanogaster. Development. 2017;144:905-915 pubmed publisher
	Tognon E, Kobia F, Busi I, Fumagalli A, De Masi F, Vaccari T. Control of lysosomal biogenesis and Notch-dependent tissue patterning by components of the TFEB-V-ATPase axis in Drosophila melanogaster. Autophagy. 2016;12:499-514 pubmed publisher
	Reimels T, Pfleger C. Drosophila Rabex-5 restricts Notch activity in hematopoietic cells and maintains hematopoietic homeostasis. J Cell Sci. 2015;128:4512-25 pubmed publisher
	Troost T, Schneider M, Klein T. A re-examination of the selection of the sensory organ precursor of the bristle sensilla of Drosophila melanogaster. PLoS Genet. 2015;11:e1004911 pubmed publisher
	Morelli E, Ginefra P, Mastrodonato V, Beznoussenko G, Rusten T, Bilder D, et al. Multiple functions of the SNARE protein Snap29 in autophagy, endocytic, and exocytic trafficking during epithelial formation in Drosophila. Autophagy. 2014;10:2251-68 pubmed publisher
	Couturier L, Trylinski M, Mazouni K, Darnet L, Schweisguth F. A fluorescent tagging approach in Drosophila reveals late endosomal trafficking of Notch and Sanpodo. J Cell Biol. 2014;207:351-63 pubmed publisher
	Ignesti M, Barraco M, Nallamothu G, Woolworth J, Duchi S, Gargiulo G, et al. Notch signaling during development requires the function of awd, the Drosophila homolog of human metastasis suppressor gene Nm23. BMC Biol. 2014;12:12 pubmed publisher
	Scafidi J, Hammond T, Scafidi S, Ritter J, Jablonska B, Roncal M, et al. Intranasal epidermal growth factor treatment rescues neonatal brain injury. Nature. 2014;506:230-4 pubmed publisher
	Angulo Rojo C, Manning Cela R, Aguirre A, Ortega A, Lopez Bayghen E. Involvement of the Notch pathway in terminal astrocytic differentiation: role of PKA. ASN Neuro. 2013;5:e00130 pubmed publisher
	Schaaf C, Misulovin Z, Gause M, Koenig A, Dorsett D. The Drosophila enhancer of split gene complex: architecture and coordinate regulation by notch, cohesin, and polycomb group proteins. G3 (Bethesda). 2013;3:1785-94 pubmed publisher
	Sachan N, Mishra A, Mutsuddi M, Mukherjee A. The Drosophila importin-α3 is required for nuclear import of notch in vivo and it displays synergistic effects with notch receptor on cell proliferation. PLoS ONE. 2013;8:e68247 pubmed publisher
	Whiteman P, de Madrid B, Taylor P, Li D, Heslop R, Viticheep N, et al. Molecular basis for Jagged-1/Serrate ligand recognition by the Notch receptor. J Biol Chem. 2013;288:7305-12 pubmed publisher
	Gómez J, Wang Y, Riechmann V. Tao controls epithelial morphogenesis by promoting Fasciclin 2 endocytosis. J Cell Biol. 2012;199:1131-43 pubmed publisher
	Kulkarni V, Khadilkar R, Magadi S, Srivathsa M, Inamdar M. Asrij maintains the stem cell niche and controls differentiation during Drosophila lymph gland hematopoiesis. PLoS ONE. 2011;6:e27667 pubmed publisher
	Tajiri R, Misaki K, Yonemura S, Hayashi S. Joint morphology in the insect leg: evolutionary history inferred from Notch loss-of-function phenotypes in Drosophila. Development. 2011;138:4621-6 pubmed publisher
	Djiane A, Shimizu H, Wilkin M, Mazleyrat S, Jennings M, Avis J, et al. Su(dx) E3 ubiquitin ligase-dependent and -independent functions of polychaetoid, the Drosophila ZO-1 homologue. J Cell Biol. 2011;192:189-200 pubmed publisher
	Boyles R, Lantz K, Poertner S, Georges S, Andres A. Presenilin controls CBP levels in the adult Drosophila central nervous system. PLoS ONE. 2010;5:e14332 pubmed publisher
	Quijano J, Stinchfield M, Zerlanko B, Gibbens Y, Takaesu N, Hyman Walsh C, et al. The Sno oncogene antagonizes Wingless signaling during wing development in Drosophila. PLoS ONE. 2010;5:e11619 pubmed publisher
	Benhra N, Vignaux F, Dussert A, Schweisguth F, Le Borgne R. Neuralized promotes basal to apical transcytosis of delta in epithelial cells. Mol Biol Cell. 2010;21:2078-86 pubmed publisher
	Genevet A, Wehr M, Brain R, Thompson B, Tapon N. Kibra is a regulator of the Salvador/Warts/Hippo signaling network. Dev Cell. 2010;18:300-8 pubmed publisher
	Matsuura A, Ito M, Sakaidani Y, Kondo T, Murakami K, Furukawa K, et al. O-linked N-acetylglucosamine is present on the extracellular domain of notch receptors. J Biol Chem. 2008;283:35486-95 pubmed publisher
	Bland C, Rand M. Methylmercury induces activation of Notch signaling. Neurotoxicology. 2006;27:982-91 pubmed
	Mukherjee A, Veraksa A, Bauer A, Rosse C, Camonis J, Artavanis Tsakonas S. Regulation of Notch signalling by non-visual beta-arrestin. Nat Cell Biol. 2005;7:1191-201 pubmed
	Kamimura K, Rhodes J, Ueda R, McNeely M, Shukla D, Kimata K, et al. Regulation of Notch signaling by Drosophila heparan sulfate 3-O sulfotransferase. J Cell Biol. 2004;166:1069-79 pubmed
	Rawlins E, Lovegrove B, Jarman A. Echinoid facilitates Notch pathway signalling during Drosophila neurogenesis through functional interaction with Delta. Development. 2003;130:6475-84 pubmed
	Reed R. Evidence for Notch-mediated lateral inhibition in organizing butterfly wing scales. Dev Genes Evol. 2004;214:43-6 pubmed
	Hu Y, Fortini M. Different cofactor activities in gamma-secretase assembly: evidence for a nicastrin-Aph-1 subcomplex. J Cell Biol. 2003;161:685-90 pubmed
	Okajima T, Irvine K. Regulation of notch signaling by o-linked fucose. Cell. 2002;111:893-904 pubmed
	Goto S, Taniguchi M, Muraoka M, Toyoda H, Sado Y, Kawakita M, et al. UDP-sugar transporter implicated in glycosylation and processing of Notch. Nat Cell Biol. 2001;3:816-22 pubmed
	Lawrence N, Klein T, Brennan K, Martinez Arias A. Structural requirements for notch signalling with delta and serrate during the development and patterning of the wing disc of Drosophila. Development. 2000;127:3185-95 pubmed
	Parks A, Klueg K, Stout J, Muskavitch M. Ligand endocytosis drives receptor dissociation and activation in the Notch pathway. Development. 2000;127:1373-85 pubmed
	Rand M, Grimm L, Artavanis Tsakonas S, Patriub V, Blacklow S, Sklar J, et al. Calcium depletion dissociates and activates heterodimeric notch receptors. Mol Cell Biol. 2000;20:1825-35 pubmed
	Ye Y, Fortini M. Apoptotic activities of wild-type and Alzheimer's disease-related mutant presenilins in Drosophila melanogaster. J Cell Biol. 1999;146:1351-64 pubmed
	Ye Y, Lukinova N, Fortini M. Neurogenic phenotypes and altered Notch processing in Drosophila Presenilin mutants. Nature. 1999;398:525-9 pubmed
	Lecourtois M, Schweisguth F. Indirect evidence for Delta-dependent intracellular processing of notch in Drosophila embryos. Curr Biol. 1998;8:771-4 pubmed
	Blahovec J. Stress relaxation in cherry fruit. Biorheology. 1996;33:451-62 pubmed
	Fehon R, Kooh P, Rebay I, Regan C, Xu T, Muskavitch M, et al. Molecular interactions between the protein products of the neurogenic loci Notch and Delta, two EGF-homologous genes in Drosophila. Cell. 1990;61:523-34 pubmed
	Fehon R, Johansen K, Rebay I, Artavanis Tsakonas S. Complex cellular and subcellular regulation of notch expression during embryonic and imaginal development of Drosophila: implications for notch function. J Cell Biol. 1991;113:657-69 pubmed
	Xu T, Caron L, Fehon R, Artavanis Tsakonas S. The involvement of the Notch locus in Drosophila oogenesis. Development. 1992;115:913-22 pubmed

product information

Internal ID :

270

Name :

C17.9C6

Depositor Name :

Artavanis-Tsakonas, S.

Depositor Institution :

Harvard Medical School

Date Deposited :

5/2/00

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Notch, intracellular domain

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

2185893

Collections :

Cardiac development,Cell adhesion,Cell markers,Cell signaling,Drosophila antigens,Neurodevelopment,Skeletal muscle,Transcription factors

Search Keywords :

Artavanis-Tsakonas, S., Notch, intracellular domain, Drosophila, MIgG1, kappa light chain, Drosophila, N, CG3936, Notch, NECD, NICD, AB_528410, Monoclonal, Drosophila antigens/Cell markers/Transcription factors/Neurodevelopment/Cell signaling/Cardiac development/Cell adhesion/Skeletal muscle, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 289 kDa; Apparent: 300 kDa and C-terminal fragments of 120 kDa and lower MW, resulting from proteolysis.

Gene :

Alternate Gene Name(s) :

CG3936, Notch, NECD, NICD

Uniprot ID :

P07207

Antibody Registry ID :

AB_528410

Immunogen :

Recombinant fusion protein containing aa 1791-2504 of the intracellular domain of Notch.

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

Ag8

Epitope Mapped :

Yes

Epitope Location or Sequence :

intracellular domain, aa 1791-2504.

Epitope Map PubMed ID :

2185893

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Excellent

Immunohistochemistry Pubmed IDs :

1451667 10704384 31422102

Immunofluorescence Pubmed IDs :

11533661 12526814 20410139 10206647 14618402 1451667 2016340 16284625 10704384 10669757 10862754 14627723 24286475 9093440

Western Blot Pubmed IDs :

2185893 11533661 10206647 9651681 16284625 18948267 10669757 10491396 16757030 24286475

Immunoprecipitation Pubmed IDs :

16284625 10669757

Additional Information :

The Neurogenic locus Notch protein functions as a transmembrane receptor for membrane-bound ligands Delta and Serrate to regulate neurodevelopment. Upon ligand activation, the intracellular domain (NICD) is released from the cell membrane, and forms a transcriptional activator complex with the Suppressor of hairless protein.

DSHB Growth Medium :

RPMI

References (legacy) :

Cell 61, 523-534.; Nature Cell Biol. 3(9), 816-822.; Cell 111, 893-904.; Mole. Biol. Cell 21, 2078-2086.

more info or order :

Developmental Studies Hybridoma Bank product webpage