Broad (core) | Developmental Studies Hybridoma Bank Broad-core (25E9.D7) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Broad (core)

catalog :

Broad-core (25E9.D7)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Broad-core (25E9.D7)

reactivity :

fruit fly

application :

western blot, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 11

Reference
Fregoso Lomas M, De Vito S, Boisclair Lachance J, Houde J, Nilson L. Determination of EGFR Signaling Output by Opposing Gradients of BMP and JAK/STAT Activity. Curr Biol. 2016;26:2572-2582 pubmed publisher
Zeng X, Hou S. Broad relays hormone signals to regulate stem cell differentiation in Drosophila midgut during metamorphosis. Development. 2012;139:3917-25 pubmed publisher
Gancz D, Lengil T, Gilboa L. Coordinated regulation of niche and stem cell precursors by hormonal signaling. PLoS Biol. 2011;9:e1001202 pubmed publisher
Abdou M, Peng C, Huang J, Zyaan O, Wang S, Li S, et al. Wnt signaling cross-talks with JH signaling by suppressing Met and gce expression. PLoS ONE. 2011;6:e26772 pubmed publisher
Huang J, Tian L, Peng C, Abdou M, Wen D, Wang Y, et al. DPP-mediated TGFbeta signaling regulates juvenile hormone biosynthesis by activating the expression of juvenile hormone acid methyltransferase. Development. 2011;138:2283-91 pubmed publisher
Spokony R, Restifo L. Broad Complex isoforms have unique distributions during central nervous system metamorphosis in Drosophila melanogaster. J Comp Neurol. 2009;517:15-36 pubmed publisher
Mirth C, Truman J, Riddiford L. The ecdysone receptor controls the post-critical weight switch to nutrition-independent differentiation in Drosophila wing imaginal discs. Development. 2009;136:2345-53 pubmed publisher
Cáceres L, Nilson L. Production of gurken in the nurse cells is sufficient for axis determination in the Drosophila oocyte. Development. 2005;132:2345-53 pubmed
Brennan C, Li T, Bender M, Hsiung F, Moses K. Broad-complex, but not ecdysone receptor, is required for progression of the morphogenetic furrow in the Drosophila eye. Development. 2001;128:1-11 pubmed
Buszczak M, Freeman M, Carlson J, Bender M, Cooley L, Segraves W. Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila. Development. 1999;126:4581-9 pubmed
Emery I, Bedian V, Guild G. Differential expression of Broad-Complex transcription factors may forecast tissue-specific developmental fates during Drosophila metamorphosis. Development. 1994;120:3275-87 pubmed

product information

Internal ID :

3759

Name :

Broad-core (25E9.D7)

Depositor Name :

Guild, G.

Depositor Institution :

University of Pennsylvania

Date Deposited :

10/15/03

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Broad (core)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

7720567

Collections :

Cell markers,Drosophila antigens,Nucleus,Transcription factors

Search Keywords :

Guild, G., Broad (core), Drosophila, MIgG1, kappa light chain, Drosophila, br, CG11509; BR-C; BR-C Z1; Br-C Z2; Br-C-Z3; npr1; PP1; PP2; rbp; rdp; rds; uq, AB_528104, Monoclonal, Drosophila antigens/Cell markers/Transcription factors/Nucleus, Immunofluorescence/Western Blot

Antigen Molecular Weight :

There are 6 reported isoforms. Predicted: 55, 70, 72, 74, 77, and 92 kDa; Apparent: 57, 64, 81, 91, and 118 kDa

Gene :

Alternate Gene Name(s) :

CG11509; BR-C; BR-C Z1; Br-C Z2; Br-C-Z3; npr1; PP1; PP2; rbp; rdp; rds; uq

Uniprot ID :

Q01295

Antibody Registry ID :

AB_528104

Immunogen :

Recombinant, His-tagged fusion protein derived from cDNA clone BRcore-Z2 corresponding to isoform 4 of BR-C [Uniprot ID Q01295-4]

Immunogen Sequence :

Total protein

Clonality :

Monoclonal

Myeloma Strain :

Sp2/0-Ag14

Epitope Mapped :

Yes

Epitope Location or Sequence :

The CORE region common to all isoforms (aa 1-431)

Epitope Map PubMed ID :

7720567

Recommended Applications :

Immunofluorescence,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

7720567 15829517 10498692 11092806 19711379 23048182 19515698 22131903 22087234 21558376 27593379

Western Blot Pubmed IDs :

7720567 10498692 22087234 21558376

Additional Information :

Binds to all 6 isoforms of the broad protein complex of transcription factors (BR-C).

DSHB Growth Medium :

Iscove's

References (legacy) :

Development 120, 3275-3287.; Development 132, 2345-2353.

more info or order :

Developmental Studies Hybridoma Bank product webpage