Broad (Z3) | Developmental Studies Hybridoma Bank Broad-Z3 (Z3.9A7) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Broad (Z3)

catalog :

Broad-Z3 (Z3.9A7)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Broad-Z3 (Z3.9A7)

reactivity :

fruit fly

application :

western blot, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 4

Reference
Zhang C, Robinson B, Xu W, Yang L, Yao B, Zhao H, et al. The ecdysone receptor coactivator Taiman links Yorkie to transcriptional control of germline stem cell factors in somatic tissue. Dev Cell. 2015;34:168-80 pubmed publisher
Spokony R, Restifo L. Broad Complex isoforms have unique distributions during central nervous system metamorphosis in Drosophila melanogaster. J Comp Neurol. 2009;517:15-36 pubmed publisher
Brennan C, Li T, Bender M, Hsiung F, Moses K. Broad-complex, but not ecdysone receptor, is required for progression of the morphogenetic furrow in the Drosophila eye. Development. 2001;128:1-11 pubmed
Emery I, Bedian V, Guild G. Differential expression of Broad-Complex transcription factors may forecast tissue-specific developmental fates during Drosophila metamorphosis. Development. 1994;120:3275-87 pubmed

product information

Internal ID :

3783

Name :

Broad-Z3 (Z3.9A7)

Depositor Name :

Guild, G.

Depositor Institution :

University of Pennsylvania

Date Deposited :

10/15/03

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Broad (Z3)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG2a, kappa light chain

Isotype for catalog (legacy) :

IgG2a, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

7720567

Collections :

Cell markers,Drosophila antigens,Nucleus,Transcription factors

Search Keywords :

Guild, G., Broad (Z3), Drosophila, MIgG2a, kappa light chain, Drosophila, br, CG11509; BR-C; BR-C Z1; Br-C Z2; Br-C-Z3; npr1; PP1; PP2; rbp; rdp; rds; uq, AB_528106, Monoclonal, Drosophila antigens/Cell markers/Transcription factors/Nucleus, Immunofluorescence/Western Blot

Antigen Molecular Weight :

Predicted: 74 kDa; Apparent: 91 kDa

Gene :

Alternate Gene Name(s) :

CG11509; BR-C; BR-C Z1; Br-C Z2; Br-C-Z3; npr1; PP1; PP2; rbp; rdp; rds; uq

Uniprot ID :

Q01295

Antibody Registry ID :

AB_528106

Immunogen :

Recombinant, His-tagged fusion protein derived from cDNA clone Z3 corresponding to aa 516-702 of isoform 5 of BR-C [Uniprot ID Q01295-5]

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

Sp2/0-Ag14

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 516-702 of isoform 5 [Uniprot ID Q01295-5]

Epitope Map PubMed ID :

7720567

Recommended Applications :

Immunofluorescence,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

11092806 26143992 19711379

Western Blot Pubmed IDs :

7720567

Additional Information :

Specific for the Z3 isoform of the broad protein complex of transcription factors. There is one Z3 BR-C isoform reported.

DSHB Growth Medium :

Iscove's

References (legacy) :

Development 120, 3275-3287.

more info or order :

Developmental Studies Hybridoma Bank product webpage