Broad (Z1) | Developmental Studies Hybridoma Bank Broad-Z1 (Z1.3C11.OA1) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Broad (Z1)

catalog :

Broad-Z1 (Z1.3C11.OA1)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Broad-Z1 (Z1.3C11.OA1)

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 12

Reference
Huang Y, Lu Y, Wu J, Chien C, Pi H. The COP9 signalosome converts temporal hormone signaling to spatial restriction on neural competence. PLoS Genet. 2014;10:e1004760 pubmed publisher
Ihry R, Sapiro A, Bashirullah A. Translational control by the DEAD Box RNA helicase belle regulates ecdysone-triggered transcriptional cascades. PLoS Genet. 2012;8:e1003085 pubmed publisher
Gancz D, Lengil T, Gilboa L. Coordinated regulation of niche and stem cell precursors by hormonal signaling. PLoS Biol. 2011;9:e1001202 pubmed publisher
Spokony R, Restifo L. Broad Complex isoforms have unique distributions during central nervous system metamorphosis in Drosophila melanogaster. J Comp Neurol. 2009;517:15-36 pubmed publisher
Prabhat -, Forsberg A, Katzourin M, Wharton K, Slater M. A comparative study of Desktop, Fishtank, and Cave systems for the exploration of volume rendered confocal data sets. IEEE Trans Vis Comput Graph. 2008;14:551-63 pubmed
Shravage B, Altmann G, Technau M, Roth S. The role of Dpp and its inhibitors during eggshell patterning in Drosophila. Development. 2007;134:2261-71 pubmed
Jordan K, Hatfield S, Tworoger M, Ward E, Fischer K, Bowers S, et al. Genome wide analysis of transcript levels after perturbation of the EGFR pathway in the Drosophila ovary. Dev Dyn. 2005;232:709-24 pubmed
Brennan C, Li T, Bender M, Hsiung F, Moses K. Broad-complex, but not ecdysone receptor, is required for progression of the morphogenetic furrow in the Drosophila eye. Development. 2001;128:1-11 pubmed
Schubiger M, Truman J. The RXR ortholog USP suppresses early metamorphic processes in Drosophila in the absence of ecdysteroids. Development. 2000;127:1151-9 pubmed
Brennan C, Ashburner M, Moses K. Ecdysone pathway is required for furrow progression in the developing Drosophila eye. Development. 1998;125:2653-64 pubmed
Sandstrom D, Bayer C, Fristrom J, Restifo L. Broad-complex transcription factors regulate thoracic muscle attachment in Drosophila. Dev Biol. 1997;181:168-85 pubmed
Emery I, Bedian V, Guild G. Differential expression of Broad-Complex transcription factors may forecast tissue-specific developmental fates during Drosophila metamorphosis. Development. 1994;120:3275-87 pubmed

product information

Internal ID :

3771

Name :

Broad-Z1 (Z1.3C11.OA1)

Depositor Name :

Guild, G.

Depositor Institution :

University of Pennsylvania

Date Deposited :

10/15/03

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Broad (Z1)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

7720567

Collections :

Cell markers,Drosophila antigens,Nucleus,Transcription factors

Search Keywords :

Guild, G., Broad (Z1), Drosophila, MIgG1, kappa light chain, Drosophila, br, CG11509; BR-C; BR-C Z1; Br-C Z2; Br-C-Z3; npr1; PP1; PP2; rbp; rdp; rds; uq, AB_528105, Monoclonal, Drosophila antigens/Cell markers/Transcription factors/Nucleus, Immunohistochemistry/Immunofluorescence/Western Blot

Antigen Molecular Weight :

Threre are 3 reported isoforms for Z1. Predicted: 70, 72, and 77 kDa; Apparent: 81 and 91 kDa

Gene :

Alternate Gene Name(s) :

CG11509; BR-C; BR-C Z1; Br-C Z2; Br-C-Z3; npr1; PP1; PP2; rbp; rdp; rds; uq

Uniprot ID :

Q01295

Antibody Registry ID :

AB_528105

Immunogen :

Recombinant, His-tagged fusion protein derived from cDNA clone BRcore-Q-Z1 corresponding to isoform 2 of BR-C [Uniprot ID Q01295-2]

Immunogen Sequence :

Total protein

Clonality :

Monoclonal

Myeloma Strain :

Sp2/0-Ag14

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 432-572 of isoform 2 [Uniprot ID Q01295-2] which is common to all three Z1 isoforms

Epitope Map PubMed ID :

7720567

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

9636080

Immunofluorescence Pubmed IDs :

9636080 11092806 9013928 18461742 23209440 17507396 15704171 10683169 19711379 22131903 25393278

Western Blot Pubmed IDs :

7720567

Additional Information :

Specific for the Z1 isoforms of the broad protein complex of transcription factors. There are three reported Z1 BR-C isoforms.

DSHB Growth Medium :

Iscove's

References (legacy) :

Development 120, 3275-3287.

more info or order :

Developmental Studies Hybridoma Bank product webpage