neuroglian (Drosophila, neuron-specific) | Developmental Studies Hybridoma Bank BP 104 anti-Neuroglian product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

neuroglian (Drosophila, neuron-specific)

catalog :

BP 104 anti-Neuroglian

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BP 104 anti-Neuroglian

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 20

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; 1:10; loading ...; fig 2c	Hartenstein V, Omoto J, Ngo K, Wong D, Kuert P, Reichert H, et al. Structure and development of the subesophageal zone of the Drosophila brain. I. Segmental architecture, compartmentalization, and lineage anatomy. J Comp Neurol. 2018;526:6-32 pubmed publisher
immunohistochemistry; fruit fly; 1:10; loading ...; fig 6e	Lovick J, Omoto J, Ngo K, Hartenstein V. Development of the anterior visual input pathway to the Drosophila central complex. J Comp Neurol. 2017;525:3458-3475 pubmed publisher
immunohistochemistry; fruit fly; 1:10; loading ...; fig 4d	Hartenstein V, Cruz L, Lovick J, Guo M. Developmental analysis of the dopamine-containing neurons of the Drosophila brain. J Comp Neurol. 2017;525:363-379 pubmed publisher
	Kendroud S, Bohra A, Kuert P, Nguyen B, Guillermin O, Sprecher S, et al. Structure and development of the subesophageal zone of the Drosophila brain. II. Sensory compartments. J Comp Neurol. 2018;526:33-58 pubmed publisher
	Shepherd D, Harris R, Williams D, Truman J. Postembryonic lineages of the Drosophila ventral nervous system: Neuroglian expression reveals the adult hemilineage associated fiber tracts in the adult thoracic neuromeres. J Comp Neurol. 2016;524:2677-95 pubmed publisher
	Peterson S, Krasnow M. Subcellular trafficking of FGF controls tracheal invasion of Drosophila flight muscle. Cell. 2015;160:313-23 pubmed publisher
	Lu C, Zhai B, Mauss A, Landgraf M, Gygi S, Van Vactor D. MicroRNA-8 promotes robust motor axon targeting by coordinate regulation of cell adhesion molecules during synapse development. Philos Trans R Soc Lond B Biol Sci. 2014;369: pubmed publisher
	Gross G, Lone G, Leung L, Hartenstein V, Guo M. X11/Mint genes control polarized localization of axonal membrane proteins in vivo. J Neurosci. 2013;33:8575-86 pubmed publisher
	Pfeiffer B, Ngo T, Hibbard K, Murphy C, Jenett A, Truman J, et al. Refinement of tools for targeted gene expression in Drosophila. Genetics. 2010;186:735-55 pubmed publisher
	Sepp K, Hong P, Lizarraga S, Liu J, Mejia L, Walsh C, et al. Identification of neural outgrowth genes using genome-wide RNAi. PLoS Genet. 2008;4:e1000111 pubmed publisher
	Yamamoto M, Ueda R, Takahashi K, Saigo K, Uemura T. Control of axonal sprouting and dendrite branching by the Nrg-Ank complex at the neuron-glia interface. Curr Biol. 2006;16:1678-83 pubmed
	Islam R, Wei S, Chiu W, Hortsch M, Hsu J. Neuroglian activates Echinoid to antagonize the Drosophila EGF receptor signaling pathway. Development. 2003;130:2051-9 pubmed
	Baumann O. Posterior midgut epithelial cells differ in their organization of the membrane skeleton from other drosophila epithelia. Exp Cell Res. 2001;270:176-87 pubmed
	Baker N, Yu S. The EGF receptor defines domains of cell cycle progression and survival to regulate cell number in the developing Drosophila eye. Cell. 2001;104:699-708 pubmed
	Bouley M, Tian M, Paisley K, Shen Y, Malhotra J, Hortsch M. The L1-type cell adhesion molecule neuroglian influences the stability of neural ankyrin in the Drosophila embryo but not its axonal localization. J Neurosci. 2000;20:4515-23 pubmed
	Koushika S, Lisbin M, White K. ELAV, a Drosophila neuron-specific protein, mediates the generation of an alternatively spliced neural protein isoform. Curr Biol. 1996;6:1634-41 pubmed
	Jacobs J. Perturbed glial scaffold formation precedes axon tract malformation in Drosophila mutants. J Neurobiol. 1993;24:611-26 pubmed
	Hortsch M, Wang Y, Marikar Y, Bieber A. The cytoplasmic domain of the Drosophila cell adhesion molecule neuroglian is not essential for its homophilic adhesive properties in S2 cells. J Biol Chem. 1995;270:18809-17 pubmed
	Hafen E, Basler K. Specification of cell fate in the developing eye of Drosophila. Dev Suppl. 1991;1:123-30 pubmed
	Hortsch M, Bieber A, Patel N, Goodman C. Differential splicing generates a nervous system-specific form of Drosophila neuroglian. Neuron. 1990;4:697-709 pubmed

product information

Internal ID :

3628

Name :

BP 104 anti-Neuroglian

Depositor Name :

Goodman, C.

Depositor Institution :

University of California, Berkeley

Date Deposited :

2/1/94

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

neuroglian (Drosophila, neuron-specific)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Drosophila

Initial Publication Pubmed ID :

1693086

Depositor Notes (Special Instructions) :

Differential splicing generates a nervous system-specific form of neuroglian. The antibody recognizes a specific form of neuroglian via a cytoplasmic domain generated by alternative splicing. Staining is first seen at about 6 hrs in a subset of neurons, but rapidly expands to include all neurons of the CNS and PNS. It is an exceptionally useful marker for the neurons of the neuron pattern in the PNS and developing eye disc. The PNS staining includes a small number of non-neuronal support cells such as the innermost sheath cell associated with the chordotonal neurons. This antibody works well on Westerns.

Collections :

Cell adhesion,Cell markers,Drosophila antigens,Neurodevelopment

Search Keywords :

Goodman, C., neuroglian (Drosophila, neuron-specific), Drosophila, MIgG1, Drosophila, Nrg, Nrg180; CG1634, AB_528402, Monoclonal, Drosophila antigens/Cell markers/Neurodevelopment/cell adhesion, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted 144 kDa; Apparent: 180 kDa

Gene :

Nrg

Alternate Gene Name(s) :

Nrg180; CG1634

Uniprot ID :

P20241

Antibody Registry ID :

AB_528402

Immunogen :

Membrane protein preparation from cultured embryonic Drosophila neurons.

Immunogen Sequence :

Total protein

Clonality :

Monoclonal

Myeloma Strain :

NS-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

Cytoplasmic domain; aa 1236-1302 of isoform B of Uniprot ID P20241

Epitope Map PubMed ID :

1693086

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

1693086 10844021 26878258 8326301

Immunofluorescence Pubmed IDs :

11640882 11257224 25135978 8994828 26878258 20697123 16920632 23658195 25557078 1742497 28875566 26878258 30871987 31006568

Western Blot Pubmed IDs :

1693086 7642532 12668620

Immunoprecipitation Pubmed IDs :

1693086

Pubmed IDs :

25135978

Additional Information :

BP 104 requires detergent permeabilization of tissue to bind to the protein, suggesting that it recognizes an epitope specific to the cytoplasmic domain of this form (isoform B) of the protein [PMID 1693086].

DSHB Growth Medium :

Iscove's

References (legacy) :

Neuron 4, 697-709.; Exp. Cell Res. 270, 176-187.; Cell 104, 699-708.; Phil. Trans. R. Soc. B doi:10.1098/rstb.2013.0517.

more info or order :

Developmental Studies Hybridoma Bank product webpage