integrin alpha-5 | Developmental Studies Hybridoma Bank BIIG2 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

integrin alpha-5

catalog :

BIIG2

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

BIIG2

reactivity :

human, cow

application :

western blot, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 18

Reference
Bekier M, Wang L, Li J, Huang H, Tang D, Zhang X, et al. Knockout of the Golgi stacking proteins GRASP55 and GRASP65 impairs Golgi structure and function. Mol Biol Cell. 2017;28:2833-2842 pubmed publisher
Agarwal R, GonzÃ¡lez GarcÃa C, Torstrick B, Guldberg R, SalmerÃ³n SÃ¡nchez M, GarcÃa A. Simple coating with fibronectin fragment enhances stainless steel screw osseointegration in healthy and osteoporotic rats. Biomaterials. 2015;63:137-45 pubmed publisher
Taylor Weiner H, Ravi N, Engler A. Traction forces mediated by integrin signaling are necessary for definitive endoderm specification. J Cell Sci. 2015;128:1961-8 pubmed publisher
Ahmadi A, McNeill B, Vulesevic B, Kordos M, Mesana L, Thorn S, et al. The role of integrin ?2 in cell and matrix therapy that improves perfusion, viability and function of infarcted myocardium. Biomaterials. 2014;35:4749-58 pubmed publisher
Gorrell R, Guan J, Xin Y, Tafreshi M, Hutton M, McGuckin M, et al. A novel NOD1- and CagA-independent pathway of interleukin-8 induction mediated by the Helicobacter pylori type IV secretion system. Cell Microbiol. 2013;15:554-70 pubmed publisher
Cox R, Livesay S, Johnson M, Ohi M, Williams J. The human metapneumovirus fusion protein mediates entry via an interaction with RGD-binding integrins. J Virol. 2012;86:12148-60 pubmed publisher
Gupton S, Riquelme D, Hughes Alford S, Tadros J, Rudina S, Hynes R, et al. Mena binds ?5 integrin directly and modulates ?5?1 function. J Cell Biol. 2012;198:657-76 pubmed publisher
Kariya Y, Gu J. N-glycosylation of ß4 integrin controls the adhesion and motility of keratinocytes. PLoS ONE. 2011;6:e27084 pubmed publisher
Isaji T, Kariya Y, Xu Q, Fukuda T, Taniguchi N, Gu J. Functional roles of the bisecting GlcNAc in integrin-mediated cell adhesion. Methods Enzymol. 2010;480:445-59 pubmed publisher
Hutton M, Kaparakis Liaskos M, Turner L, Cardona A, Kwok T, Ferrero R. Helicobacter pylori exploits cholesterol-rich microdomains for induction of NF-kappaB-dependent responses and peptidoglycan delivery in epithelial cells. Infect Immun. 2010;78:4523-31 pubmed publisher
Pellinen T, Arjonen A, Vuoriluoto K, Kallio K, Fransen J, Ivaska J. Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of beta1-integrins. J Cell Biol. 2006;173:767-80 pubmed
Koivisto L, Grenman R, Heino J, Larjava H. Integrins alpha5beta1, alphavbeta1, and alphavbeta6 collaborate in squamous carcinoma cell spreading and migration on fibronectin. Exp Cell Res. 2000;255:10-7 pubmed
Koivisto L, Larjava K, Hakkinen L, Uitto V, Heino J, Larjava H. Different integrins mediate cell spreading, haptotaxis and lateral migration of HaCaT keratinocytes on fibronectin. Cell Adhes Commun. 1999;7:245-57 pubmed
Huhtala P, Humphries M, McCarthy J, Tremble P, Werb Z, Damsky C. Cooperative signaling by alpha 5 beta 1 and alpha 4 beta 1 integrins regulates metalloproteinase gene expression in fibroblasts adhering to fibronectin. J Cell Biol. 1995;129:867-79 pubmed
Damsky C, Librach C, Lim K, Fitzgerald M, McMaster M, Janatpour M, et al. Integrin switching regulates normal trophoblast invasion. Development. 1994;120:3657-66 pubmed
Werb Z, Tremble P, Behrendtsen O, Crowley E, Damsky C. Signal transduction through the fibronectin receptor induces collagenase and stromelysin gene expression. J Cell Biol. 1989;109:877-89 pubmed
Hall D, Reichardt L, Crowley E, Holley B, Moezzi H, Sonnenberg A, et al. The alpha 1/beta 1 and alpha 6/beta 1 integrin heterodimers mediate cell attachment to distinct sites on laminin. J Cell Biol. 1990;110:2175-84 pubmed
Damsky C, Fitzgerald M, Fisher S. Distribution patterns of extracellular matrix components and adhesion receptors are intricately modulated during first trimester cytotrophoblast differentiation along the invasive pathway, in vivo. J Clin Invest. 1992;89:210-22 pubmed

product information

Internal ID :

2096

Name :

BIIG2

Depositor Name :

Damsky, C.H.

Depositor Institution :

University of California

Date Deposited :

12/16/97

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

integrin alpha-5

Antigen Species :

Human

Host Species :

rat

Isotype :

RIgG2b, kappa light chain

Isotype for catalog (legacy) :

IgG2b, kappa light chain

Positive Tested Species Reactivity :

Bovine,Human,Rabbit

Species Tested (legacy) :

human, rabbit, bovine

Initial Publication Pubmed ID :

2351695

Depositor Notes (Special Instructions) :

Stains aldehyde fixed cells and frozen sections of aldehyde fixed cells. Blocks cell attachment to Fibronectin only. Binds Protein G. Cell binding: stains cell surface of connective tissue cells, some embryonic epithelia. Doesn't appear to work for western blotting.

Collections :

CD antigens,Cardiac development,Cell adhesion,Cell signaling,Human,Muscular dystrophy,Neurodevelopment,Skeletal muscle

Search Keywords :

Damsky, C.H., integrin alpha-5, Human, RIgG2b, kappa light chain, human/rabbit/bovine, ITGA5, FNRA, CD49e, VLA-5, VLA5A, AB_528155, monoclonal, Cardiac development/Cell adhesion/Cell signaling/Cluster Determinant antigens/Human/Muscular dystrophy/Neurodevelopment/Skeletal muscle, Function Blocking/Immunofluorescence/Immunoprecipitation/FACS/Western Blot

Antigen Molecular Weight :

Predicted: 115 kDa; Apparent: 130 kDa non-reduced gel mobility, 120 kDa reduced gel mobility

Gene :

ITGA5

Alternate Gene Name(s) :

FNRA, CD49e, VLA-5, VLA5A

Uniprot ID :

P08648

Antibody Registry ID :

AB_528155

Synonyms (Alternate Clone Names) :

anti-FnR

Immunogen :

EDTA-harvested human JAR choriocarcinoma cells

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

SP 2/0

Epitope Mapped :

Recommended Applications :

FACS,Function Blocking,Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Immunofluorescence Pubmed IDs :

1370295 7529679 20713621

Western Blot Pubmed IDs :

24631247

Immunoprecipitation Pubmed IDs :

2547805 7537277 20816221

Function Blocking Pubmed IDs :

2547805 2351695 10626908 10666329 20713621 26100343 25908864 23107019 22933271 24631247 10809728

FACS Pubmed IDs :

10626908 10666329 22933271

Additional Information :

Like most anti-integrin antibodies, AIIB2 does not blot well. Refer to the cited paper for protocol required for western blotting.

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Clin. Invest. 89, 210-222.; Development 120, 3657-3666.; J. Cell Biol. 129, 867-880.; Cell Adhes. Commun. 7(3), 245-257.; Exp. Cell Res. 255, 10-17.; Infect. Immun. 78(11), 4523-4531.

more info or order :

Developmental Studies Hybridoma Bank product webpage