Myosin heavy chain, embryonic | Developmental Studies Hybridoma Bank BF-G6 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Myosin heavy chain, embryonic

catalog :

BF-G6

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BF-G6

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; rat; 1:200; fig s3	Moretti I, Ciciliot S, Dyar K, Abraham R, Murgia M, Agatea L, et al. MRF4 negatively regulates adult skeletal muscle growth by repressing MEF2 activity. Nat Commun. 2016;7:12397 pubmed publisher
immunohistochemistry - frozen section; mouse; fig 5	Tjondrokoesoemo A, Schips T, Kanisicak O, Sargent M, Molkentin J. Genetic overexpression of Serpina3n attenuates muscular dystrophy in mice. Hum Mol Genet. 2016;25:1192-202 pubmed publisher
	Marroncelli N, Bianchi M, Bertin M, Consalvi S, Saccone V, De Bardi M, et al. HDAC4 regulates satellite cell proliferation and differentiation by targeting P21 and Sharp1 genes. Sci Rep. 2018;8:3448 pubmed publisher
	Mizbani A, Luca E, Rushing E, Krutzfeldt J. MicroRNA deep sequencing in two adult stem cell populations identifies miR-501 as a novel regulator of myosin heavy chain during muscle regeneration. Development. 2016;143:4137-4148 pubmed
	Oliveira Fernandes M, Tourtellotte W. Egr3-dependent muscle spindle stretch receptor intrafusal muscle fiber differentiation and fusimotor innervation homeostasis. J Neurosci. 2015;35:5566-78 pubmed publisher
	Accornero F, Kanisicak O, Tjondrokoesoemo A, Attia A, McNally E, Molkentin J. Myofiber-specific inhibition of TGF? signaling protects skeletal muscle from injury and dystrophic disease in mice. Hum Mol Genet. 2014;23:6903-15 pubmed publisher
	Costa A, Toschi A, Murfuni I, Pelosi L, Sica G, Adamo S, et al. Local overexpression of V1a-vasopressin receptor enhances regeneration in tumor necrosis factor-induced muscle atrophy. Biomed Res Int. 2014;2014:235426 pubmed publisher
	Röder I, Strack S, Reischl M, Dahley O, Khan M, Kassel O, et al. Participation of myosin Va and Pka type I in the regeneration of neuromuscular junctions. PLoS ONE. 2012;7:e40860 pubmed publisher
	Rossi A, Mammucari C, Argentini C, Reggiani C, Schiaffino S. Two novel/ancient myosins in mammalian skeletal muscles: MYH14/7b and MYH15 are expressed in extraocular muscles and muscle spindles. J Physiol. 2010;588:353-64 pubmed publisher
	Schiaffino S, Gorza L, Pitton G, Saggin L, Ausoni S, Sartore S, et al. Embryonic and neonatal myosin heavy chain in denervated and paralyzed rat skeletal muscle. Dev Biol. 1988;127:1-11 pubmed
	Gorza L, Saggin L, Sartore S, Ausoni S. An embryonic-like myosin heavy chain is transiently expressed in nodal conduction tissue of the rat heart. J Mol Cell Cardiol. 1988;20:931-41 pubmed
	Schiaffino S, Gorza L, Sartore S, Saggin L, Carli M. Embryonic myosin heavy chain as a differentiation marker of developing human skeletal muscle and rhabdomyosarcoma. A monoclonal antibody study. Exp Cell Res. 1986;163:211-20 pubmed
	Bottinelli R, Schiaffino S, Reggiani C. Force-velocity relations and myosin heavy chain isoform compositions of skinned fibres from rat skeletal muscle. J Physiol. 1991;437:655-72 pubmed

product information

Internal ID :

5808

Name :

BF-G6

Depositor Name :

Schiaffino, S.

Depositor Institution :

Universita degli Studi di Padov

Depositor Website :

http://www.ae-info.org/ae/Member/Schiaffino_Stefano

Date Deposited :

3/25/09

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Myosin heavy chain, embryonic

Antigen Species :

Bovine

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Mouse,Rat

Species Tested (legacy) :

rat and mouse

Initial Publication Pubmed ID :

3002828

Depositor Notes (Special Instructions) :

BF-G6 antibody recognizes embryonic myosin heavy chain on fresh frozen or acetone-fixed cultures of rhabdomyosarcomas and Wilms' tumors.

Collections :

Cell markers,Human,Skeletal muscle

Search Keywords :

Schiaffino, Stefano , Myosin heavy chain, embryonic, Bovine, MIgG1, kappa light chain, Rat/Mouse, MYH3, HEMHC, MYHC-EMB, MYHSE1, SMHCE, AB_10571455, Monoclonal, Human/Skeletal muscle/Cell markers, Immunohistochemistry/Immunofluorescence/western blot/ELISA

Antigen Molecular Weight :

223 kDa

Gene :

MYH3

Alternate Gene Name(s) :

HEMHC, MYHC-EMB, MYHSE1, SMHCE

Uniprot ID :

P11055

Antibody Registry ID :

AB_10571455

Immunogen :

Purified myosin from embryonic bovine skeletal muscle

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS-0

Epitope Mapped :

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

3002828 3282936 26744329 1890654 3216402

Immunofluorescence Pubmed IDs :

3002828 3282936 27707793 27484840 25855173 25106553 24971321 22815846 19948655

Western Blot Pubmed IDs :

3002828 3282936 19948655

ELISA Pubmed IDs :

3002828

Pubmed IDs :

1890654

Additional Information :

RRID:AB_10571455

DSHB Growth Medium :

Iscove's

References (legacy) :

Exp. Cell Res. 163(1), 211-220.; J. Mole. Cell. Cardiol. 20(10), 931-941.; Dev. Biol. 127(1), 1-11.; J. Physiol. 437, 655-672.

more info or order :

Developmental Studies Hybridoma Bank product webpage