Myosin heavy chain, embryonic/ myosin-3 | Developmental Studies Hybridoma Bank BF-45 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Myosin heavy chain, embryonic/ myosin-3

catalog :

BF-45

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BF-45

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; rat; 1:200	Szarek M, Li R, Vikraman J, Southwell B, Hutson J. Molecular signals governing cremaster muscle development: clues for cryptorchidism. J Pediatr Surg. 2014;49:312-6; discussion 316 pubmed publisher
	Zou K, De Lisio M, Huntsman H, Pincu Y, Mahmassani Z, Miller M, et al. Laminin-111 improves skeletal muscle stem cell quantity and function following eccentric exercise. Stem Cells Transl Med. 2014;3:1013-22 pubmed publisher
	Bamford J, Lopaschuk G, Maclean I, Reinhart M, Dixon W, Putman C. Effects of chronic AICAR administration on the metabolic and contractile phenotypes of rat slow- and fast-twitch skeletal muscles. Can J Physiol Pharmacol. 2003;81:1072-82 pubmed
	Smith T, Block N, Rhodes S, Konieczny S, Miller J. A unique pattern of expression of the four muscle regulatory factor proteins distinguishes somitic from embryonic, fetal and newborn mouse myogenic cells. Development. 1993;117:1125-33 pubmed
	Schiaffino S, Gorza L, Pitton G, Saggin L, Ausoni S, Sartore S, et al. Embryonic and neonatal myosin heavy chain in denervated and paralyzed rat skeletal muscle. Dev Biol. 1988;127:1-11 pubmed
	Schiaffino S, Gorza L, Sartore S, Saggin L, Carli M. Embryonic myosin heavy chain as a differentiation marker of developing human skeletal muscle and rhabdomyosarcoma. A monoclonal antibody study. Exp Cell Res. 1986;163:211-20 pubmed

product information

Internal ID :

5813

Name :

BF-45

Depositor Name :

Schiaffino, S.

Depositor Institution :

Universita degli Studi di Padov

Depositor Website :

http://www.ae-info.org/ae/Member/Schiaffino_Stefano

Date Deposited :

3/25/09

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Myosin heavy chain, embryonic/ myosin-3

Antigen Species :

Bovine

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Mouse,Rat

Species Tested (legacy) :

rat, mouse and human

Initial Publication Pubmed ID :

3282936

Depositor Notes (Special Instructions) :

BF-G6 antibody recognizes embryonic myosin heavy chain on fresh frozen or acetone-fixed cultures of rhabdomyosarcomas and Wilms' tumors.

Collections :

Cell markers,Human,Skeletal muscle

Search Keywords :

Schiaffino, Stefano , Myosin heavy chain, embryonic/ myosin-3, Bovine, MIgG1, kappa light chain, Rat/Mouse, MYH3, HEMHC, MYHC-EMB, MYHSE1, SMHCE, AB_10573810, Monoclonal, Human/Skeletal muscle/Cell markers, Immunohistochemistry/Immunofluorescence/western blot/ Immunoprecipitation/ELISA

Antigen Molecular Weight :

223 kDa

Gene :

MYH3

Alternate Gene Name(s) :

HEMHC, MYHC-EMB, MYHSE1, SMHCE

Uniprot ID :

P11055

Antibody Registry ID :

AB_10573810

Immunogen :

Purified myosin from embryonic bovine skeletal muscle

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS-0

Epitope Mapped :

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

3282936 14719043 8391976 25015639 14719043 3002828

Immunofluorescence Pubmed IDs :

24528975

Western Blot Pubmed IDs :

3282936

Immunoprecipitation Pubmed IDs :

3282936

Additional Information :

RRID:AB_10573810

DSHB Growth Medium :

Iscove's

References (legacy) :

Dev. Biol. 127(1), 1-11.

more info or order :

Developmental Studies Hybridoma Bank product webpage