Myosin heavy chain (all but 2X) | Developmental Studies Hybridoma Bank BF-35 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Myosin heavy chain (all but 2X)

catalog :

BF-35

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BF-35

reactivity :

human, mouse, dog, cow

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 26

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:100; loading ...; fig 4a	Eshima H, Tamura Y, Kakehi S, Kurebayashi N, Murayama T, Nakamura K, et al. Long-term, but not short-term high-fat diet induces fiber composition changes and impaired contractile force in mouse fast-twitch skeletal muscle. Physiol Rep. 2017;5: pubmed publisher
immunohistochemistry; mouse; 1:2	Southard S, Kim J, Low S, Tsika R, Lepper C. Myofiber-specific TEAD1 overexpression drives satellite cell hyperplasia and counters pathological effects of dystrophin deficiency. elife. 2016;5: pubmed publisher
immunohistochemistry - frozen section; human; 1:50; fig 2	Andersen T, Schmidt J, Pedersen M, Krustrup P, Bangsbo J. The Effects of 52 Weeks of Soccer or Resistance Training on Body Composition and Muscle Function in +65-Year-Old Healthy Males--A Randomized Controlled Trial. PLoS ONE. 2016;11:e0148236 pubmed publisher
immunohistochemistry - frozen section; cow; 1:10	Phelps K, Drouillard J, Silva M, Miranda L, Ebarb S, Van Bibber Krueger C, et al. Effect of extended postmortem aging and steak location on myofibrillar protein degradation and Warner-Bratzler shear force of beef M. semitendinosus steaks. J Anim Sci. 2016;94:412-23 pubmed publisher
immunohistochemistry; mouse; fig 4	Foltz S, Modi J, Melick G, Abousaud M, Luan J, Fortunato M, et al. Abnormal Skeletal Muscle Regeneration plus Mild Alterations in Mature Fiber Type Specification in Fktn-Deficient Dystroglycanopathy Muscular Dystrophy Mice. PLoS ONE. 2016;11:e0147049 pubmed publisher
immunohistochemistry; cow	Phelps K, Drouillard J, Jennings J, Depenbusch B, Van Bibber Krueger C, Miller K, et al. Effects of the Programmed Nutrition Beef Program on meat quality characteristics. J Anim Sci. 2014;92:1780-91 pubmed publisher
	Hergenreder J, Legako J, Dinh T, Spivey K, Baggerman J, Broadway P, et al. Zinc Methionine Supplementation Impacts Gene and Protein Expression in Calf-Fed Holstein Steers with Minimal Impact on Feedlot Performance. Biol Trace Elem Res. 2016;171:315-327 pubmed publisher
	Frese S, Ruebner M, Suhr F, Konou T, Tappe K, Toigo M, et al. Long-Term Endurance Exercise in Humans Stimulates Cell Fusion of Myoblasts along with Fusogenic Endogenous Retroviral Genes In Vivo. PLoS ONE. 2015;10:e0132099 pubmed publisher
	Soukup T, Diallo M. Proportions of myosin heavy chain mRNAs, protein isoforms and fiber types in the slow and fast skeletal muscles are maintained after alterations of thyroid status in rats. Physiol Res. 2015;64:111-8 pubmed
	O Connell R, Carberry J, O Halloran K. Sternohyoid and diaphragm muscle form and function during postnatal development in the rat. Exp Physiol. 2013;98:1386-400 pubmed publisher
	Osterlund C, Liu J, Thornell L, Eriksson P. Intrafusal myosin heavy chain expression of human masseter and biceps muscles at young age shows fundamental similarities but also marked differences. Histochem Cell Biol. 2013;139:895-907 pubmed publisher
	Baligand C, Jouvion G, Schakman O, Gilson H, Wary C, Thissen J, et al. Multiparametric functional nuclear magnetic resonance imaging shows alterations associated with plasmid electrotransfer in mouse skeletal muscle. J Gene Med. 2012;14:598-608 pubmed publisher
	Bryson J, Hobbs C, Parsons M, Bosch K, Pandraud A, Walsh F, et al. Amyloid precursor protein (APP) contributes to pathology in the SOD1(G93A) mouse model of amyotrophic lateral sclerosis. Hum Mol Genet. 2012;21:3871-82 pubmed publisher
	Graziotti G, Chamizo V, Ríos C, Acevedo L, Rodríguez Menéndez J, Victorica C, et al. Adaptive functional specialisation of architectural design and fibre type characteristics in agonist shoulder flexor muscles of the llama, Lama glama. J Anat. 2012;221:151-63 pubmed publisher
	Bloemberg D, Quadrilatero J. Rapid determination of myosin heavy chain expression in rat, mouse, and human skeletal muscle using multicolor immunofluorescence analysis. PLoS ONE. 2012;7:e35273 pubmed publisher
	Duris M, Picard B, Geay Y. Specificity of different anti-myosin heavy chain antibodies in bovine muscle. Meat Sci. 2000;55:67-78 pubmed
	Zhou Y, Liu D, Kaminski H. Pitx2 regulates myosin heavy chain isoform expression and multi-innervation in extraocular muscle. J Physiol. 2011;589:4601-14 pubmed publisher
	Zhou Y, Liu D, Kaminski H. Myosin heavy chain expression in mouse extraocular muscle: more complex than expected. Invest Ophthalmol Vis Sci. 2010;51:6355-63 pubmed publisher
	Hyatt J, Roy R, Rugg S, Talmadge R. Myosin heavy chain composition of tiger (Panthera tigris) and cheetah (Acinonyx jubatus) hindlimb muscles. J Exp Zool A Ecol Genet Physiol. 2010;313:45-57 pubmed publisher
	Acevedo L, Rivero J. New insights into skeletal muscle fibre types in the dog with particular focus towards hybrid myosin phenotypes. Cell Tissue Res. 2006;323:283-303 pubmed
	Graziotti G, Palencia P, Delhon G, Rivero J. Neuromuscular partitioning, architectural design, and myosin fiber types of the M. vastus lateralis of the llama (Lama glama). J Morphol. 2004;262:667-81 pubmed
	Levine S, Kaiser L, Leferovich J, Tikunov B. Cellular adaptations in the diaphragm in chronic obstructive pulmonary disease. N Engl J Med. 1997;337:1799-806 pubmed
	Dunn S, Michel R. Coordinated expression of myosin heavy chain isoforms and metabolic enzymes within overloaded rat muscle fibers. Am J Physiol. 1997;273:C371-83 pubmed
	Rivero J, Talmadge R, Edgerton V. Myosin heavy chain isoforms in adult equine skeletal muscle: an immunohistochemical and electrophoretic study. Anat Rec. 1996;246:185-94 pubmed publisher
	Michel R, Parry D, Dunn S. Regulation of myosin heavy chain expression in adult rat hindlimb muscles during short-term paralysis: comparison of denervation and tetrodotoxin-induced neural inactivation. FEBS Lett. 1996;391:39-44 pubmed
	Schiaffino S, Gorza L, Sartore S, Saggin L, Ausoni S, Vianello M, et al. Three myosin heavy chain isoforms in type 2 skeletal muscle fibres. J Muscle Res Cell Motil. 1989;10:197-205 pubmed

product information

Internal ID :

5839

Name :

BF-35

Depositor Name :

Schiaffino, S.

Depositor Institution :

University of Padova

Depositor Website :

http://www.vimm.it/research/group.php?PI=12

Date Deposited :

3/25/09

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Myosin heavy chain (all but 2X)

Antigen Species :

Bovine

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Bovine,Canine,Equine,Feline,Goat,Guinea Pig,Human,Mole,Porcine,Rodent

Species Tested (legacy) :

rat, mouse, human, guinea pig, horse, cat, cattle, llama, goat, dog, pig

Initial Publication Pubmed ID :

2547831

Depositor Notes (Special Instructions) :

Fusion: 1986. Recognizes type 1, 2A, and 2B fibers in skeletal muscle.

Collections :

Cardiac development,Cell markers,Cytoskeleton,Human,Skeletal muscle

Search Keywords :

Schiaffino, Stefano, Myosin heavy chain (all but 2X), Bovine, MIgG1, Bovine/Porcine/Human/Rodent/Equine/Feline/Canine/Goat/Guinea Pig, MYH2, MyHC-2a, AB_2274680, monoclonal, Human/Cell markers/Cytoskeletal elements/Skeletal muscle/Cardiac development proteins, Immunohistochemistry/Immunofluorescence/Western Blot

Antigen Molecular Weight :

220kDa

Gene :

MYH2

Alternate Gene Name(s) :

MyHC-2a

Uniprot ID :

Q9BE41

Antibody Registry ID :

AB_2274680

Immunogen :

purified myosin from bovine skeletal muscle (fetal, 3 months)

Clonality :

Monoclonal

Myeloma Strain :

NS0

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

2547831 8888960 19768738 9400036 9277335 23306907 16163488 22625659 25194137 23038159 22678056 15376272 23709586 30376699

Immunofluorescence Pubmed IDs :

22060906 21727215 20610840 22530000 8706926

Western Blot Pubmed IDs :

22060906 19768738 16163488

Pubmed IDs :

16163488

Additional Information :

This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Muscle Res. Cell. Motil. 10(3), 197-205.; Cell Tissue Res. 323, 283-303. ; Asian-Aust. J. Anim. Sci. 24(1), 125-129.; J. Anat. doi:10.1111/j.1469-7580.2012.01520.x

more info or order :

Developmental Studies Hybridoma Bank product webpage