Myosin heavy chain (slow and 2A) | Developmental Studies Hybridoma Bank BF-32 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Myosin heavy chain (slow and 2A)

catalog :

BF-32

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BF-32

reactivity :

human, cow

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 4

Reference
Yates D, Cadaret C, Beede K, Riley H, Macko A, Anderson M, et al. Intrauterine growth-restricted sheep fetuses exhibit smaller hindlimb muscle fibers and lower proportions of insulin-sensitive Type I fibers near term. Am J Physiol Regul Integr Comp Physiol. 2016;310:R1020-9 pubmed publisher
Furuichi Y, Goto Inoue N, Manabe Y, Setou M, Masuda K, Fujii N. Imaging mass spectrometry reveals fiber-specific distribution of acetylcarnitine and contraction-induced carnitine dynamics in rat skeletal muscles. Biochim Biophys Acta. 2014;1837:1699-706 pubmed publisher
Borrione A, Zanellato A, Saggin L, Mazzoli M, Azzarello G, Sartore S. Neonatal myosin heavy chains are not expressed in Ni-induced rat rhabdomyosarcoma. Differentiation. 1988;38:49-59 pubmed
Schiaffino S, Gorza L, Sartore S, Saggin L, Ausoni S, Vianello M, et al. Three myosin heavy chain isoforms in type 2 skeletal muscle fibres. J Muscle Res Cell Motil. 1989;10:197-205 pubmed

product information

Internal ID :

5844

Name :

BF-32

Depositor Name :

Schiaffino, S.

Depositor Institution :

University of Padova

Depositor Website :

http://www.vimm.it/research/group.php?PI=12

Date Deposited :

3/25/09

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Myosin heavy chain (slow and 2A)

Antigen Species :

Bovine

Host Species :

mouse

Isotype :

MIgM

Isotype for catalog (legacy) :

IgM

Positive Tested Species Reactivity :

Human,Ovine,Rodent

Species Tested (legacy) :

rat, mouse, and human

Initial Publication Pubmed ID :

2547831

Depositor Notes (Special Instructions) :

Fusion: 1984. Recognizes type 1 (slow) and 2A fibers in skeletal muscle.

Collections :

Cardiac development,Cell markers,Cytoskeleton,Human,Skeletal muscle

Search Keywords :

Schiaffino, Stefano, Myosin heavy chain (slow and 2A), Bovine, MIgM, Rodent/Ovine/Human, MYH2, MyHC-2a, AB_10573811, monoclonal, Human/Cell markers/Cytoskeletal elements/Skeletal muscle/Cardiac development proteins, Immunohistochemistry/Immunofluorescence/Western Blot

Antigen Molecular Weight :

220kDa

Gene :

MYH2

Alternate Gene Name(s) :

MyHC-2a

Uniprot ID :

Q9BE41

Antibody Registry ID :

AB_10573811

Immunogen :

purified myosin from bovine skeletal muscle (fetal, 3 months)

Clonality :

Monoclonal

Myeloma Strain :

NS0

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

2547831

Immunofluorescence Pubmed IDs :

27053651 24882639

Western Blot Pubmed IDs :

2547831 3181651

Additional Information :

This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Muscle Res. Cell. Motil. 10(3), 197-205.

more info or order :

Developmental Studies Hybridoma Bank product webpage