Mature brain-derived neurotrophic factor | Developmental Studies Hybridoma Bank BDNF-#9 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Mature brain-derived neurotrophic factor

catalog :

BDNF-#9

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BDNF-#9

reactivity :

human, mouse, rat

application :

ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; loading ...; fig 2i	Rauskolb S, Andreska T, Fries S, von Collenberg C, Blum R, Monoranu C, et al. Insulin-like growth factor 5 associates with human Aß plaques and promotes cognitive impairment. Acta Neuropathol Commun. 2022;10:68 pubmed publisher
	López Benito S, Sánchez Sánchez J, Brito V, Calvo L, Lisa S, Torres Valle M, et al. Regulation of BDNF Release by ARMS/Kidins220 through Modulation of Synaptotagmin-IV Levels. J Neurosci. 2018;38:5415-5428 pubmed publisher
	Chen A, Zang K, Masliah E, Reichardt L. Glutamatergic axon-derived BDNF controls GABAergic synaptic differentiation in the cerebellum. Sci Rep. 2016;6:20201 pubmed publisher
	He W, Cui L, Zhang C, Zhang X, He J, Xie Y. Sonic Hedgehog Promotes Neurite Outgrowth of Primary Cortical Neurons Through Up-Regulating BDNF Expression. Neurochem Res. 2016;41:687-95 pubmed publisher
	Andreska T, Aufmkolk S, Sauer M, Blum R. High abundance of BDNF within glutamatergic presynapses of cultured hippocampal neurons. Front Cell Neurosci. 2014;8:107 pubmed publisher
	Van Kanegan M, He D, Dunn D, Yang P, Newman R, West A, et al. BDNF mediates neuroprotection against oxygen-glucose deprivation by the cardiac glycoside oleandrin. J Neurosci. 2014;34:963-8 pubmed publisher
	Rantamäki T, Kemppainen S, Autio H, Stavén S, Koivisto H, Kojima M, et al. The impact of Bdnf gene deficiency to the memory impairment and brain pathology of APPswe/PS1dE9 mouse model of Alzheimer's disease. PLoS ONE. 2013;8:e68722 pubmed publisher
	Deogracias R, Yazdani M, Dekkers M, Guy J, Ionescu M, Vogt K, et al. Fingolimod, a sphingosine-1 phosphate receptor modulator, increases BDNF levels and improves symptoms of a mouse model of Rett syndrome. Proc Natl Acad Sci U S A. 2012;109:14230-5 pubmed publisher
	Dieni S, Matsumoto T, Dekkers M, Rauskolb S, Ionescu M, Deogracias R, et al. BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons. J Cell Biol. 2012;196:775-88 pubmed publisher
	Segawa M, Morinobu S, Matsumoto T, Fuchikami M, Yamawaki S. Electroconvulsive seizure, but not imipramine, rapidly up-regulates pro-BDNF and t-PA, leading to mature BDNF production, in the rat hippocampus. Int J Neuropsychopharmacol. 2013;16:339-50 pubmed publisher
	Heyden A, Ionescu M, Romorini S, Kracht B, Ghiglieri V, Calabresi P, et al. Hippocampal enlargement in Bassoon-mutant mice is associated with enhanced neurogenesis, reduced apoptosis, and abnormal BDNF levels. Cell Tissue Res. 2011;346:11-26 pubmed publisher
	Matsumoto T, Rauskolb S, Polack M, Klose J, Kolbeck R, Korte M, et al. Biosynthesis and processing of endogenous BDNF: CNS neurons store and secrete BDNF, not pro-BDNF. Nat Neurosci. 2008;11:131-3 pubmed publisher
	Kolbeck R, Bartke I, Eberle W, Barde Y. Brain-derived neurotrophic factor levels in the nervous system of wild-type and neurotrophin gene mutant mice. J Neurochem. 1999;72:1930-8 pubmed

product information

Internal ID :

8174

Name :

BDNF-#9

Depositor Name :

Barde, Y.-A.

Depositor Institution :

Biozentrum University of Basel

Date Deposited :

5/2/12

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Mature brain-derived neurotrophic factor

Antigen Species :

Fish/Human

Host Species :

mouse

Isotype :

MIgG2b

Isotype for catalog (legacy) :

IgG2b

Positive Tested Species Reactivity :

Fish,Human,Mammal,Mouse,Rat

Species Tested (legacy) :

fish and mammals including human, mouse and rat

Initial Publication Pubmed ID :

10217270

Depositor Notes (Special Instructions) :

BDNF-#9 blocks the biological activity of Xyphophorus and mammalian BDNF. For immunostaining: perfusion fixation with 4% paraformaldehyde, 0.1% glutaraldehyde, 0.3% picric acid.

Collections :

Cell signaling,Human,Neurodevelopment

Search Keywords :

Barde, Y.-A., Mature brain-derived neurotrophic factor, Fish/Human, MIgG2b, fish/mammal/human/mouse/rat, BDNF, , AB_2617199, monoclonal, Cell signaling/Human/Neurodevelopment, Immunofluorescence/Immunoprecipitation/ELISA/Function Blocking

Antigen Molecular Weight :

Apparent: 14 kDa

Gene :

BDNF

Uniprot ID :

P23560

Antibody Registry ID :

AB_2617199

Synonyms (Alternate Clone Names) :

mAb#9

Immunogen :

Fish and Human BDNF

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

X63.Ag 8.635

Epitope Mapped :

Recommended Applications :

ELISA,Function Blocking,Immunofluorescence,Immunoprecipitation

Immunoblotting (legacy) :

Immunofluorescence Pubmed IDs :

22412021 24782711 26830657

Immunoprecipitation Pubmed IDs :

18204444 18204444 22412021 22891354 22310305

ELISA Pubmed IDs :

10217270 18204444 23844236 21935677 26459360

Function Blocking Pubmed IDs :

10217270 22891354 24431454

Additional Information :

BDNF-#9 does not recognize BDNF on western blots but mAb BDNF-#1 (a different clone from the same fusion; available in the DSHB collection) reacts specifically with BDNF by western blot. BDNF-#9 doesn't appear to recognize chick BDNF. This antibody also recognizes pro-BDNF. For ELISA, BDNF-#9 is often used in combination with BDNF-#1 that recognizes a different epitope on BDNF.

DSHB Growth Medium :

RPMI

References (legacy) :

J. Neurochem. 72, 1930-1938.; Nat. Neurosci. 11, 131-133.; J. Cell Biol. 196, 775-788.

more info or order :

Developmental Studies Hybridoma Bank product webpage