Mature brain-derived neurotrophic factor | Developmental Studies Hybridoma Bank BDNF-#1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Mature brain-derived neurotrophic factor

catalog :

BDNF-#1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BDNF-#1

reactivity :

human, mouse, rat

application :

western blot, ELISA

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 5

Reference
López Benito S, Sánchez Sánchez J, Brito V, Calvo L, Lisa S, Torres Valle M, et al. Regulation of BDNF Release by ARMS/Kidins220 through Modulation of Synaptotagmin-IV Levels. J Neurosci. 2018;38:5415-5428 pubmed publisher
Rantamäki T, Kemppainen S, Autio H, Stavén S, Koivisto H, Kojima M, et al. The impact of Bdnf gene deficiency to the memory impairment and brain pathology of APPswe/PS1dE9 mouse model of Alzheimer's disease. PLoS ONE. 2013;8:e68722 pubmed publisher
Heyden A, Ionescu M, Romorini S, Kracht B, Ghiglieri V, Calabresi P, et al. Hippocampal enlargement in Bassoon-mutant mice is associated with enhanced neurogenesis, reduced apoptosis, and abnormal BDNF levels. Cell Tissue Res. 2011;346:11-26 pubmed publisher
Matsumoto T, Rauskolb S, Polack M, Klose J, Kolbeck R, Korte M, et al. Biosynthesis and processing of endogenous BDNF: CNS neurons store and secrete BDNF, not pro-BDNF. Nat Neurosci. 2008;11:131-3 pubmed publisher
Kolbeck R, Bartke I, Eberle W, Barde Y. Brain-derived neurotrophic factor levels in the nervous system of wild-type and neurotrophin gene mutant mice. J Neurochem. 1999;72:1930-8 pubmed

product information

Internal ID :

8178

Name :

BDNF-#1

Depositor Name :

Barde, Y.-A.

Depositor Institution :

Biozentrum University of Basel

Date Deposited :

5/2/12

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Mature brain-derived neurotrophic factor

Antigen Species :

Fish/Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human,Mammal,Mouse,Rat

Species Tested (legacy) :

mammals including human, mouse and rat

Initial Publication Pubmed ID :

10217270

Depositor Notes (Special Instructions) :

This antibody doesn't work for Immunostaining nor function blocking. Works for immunoprecipitation but BDNF-#9 is preferred.

Collections :

Cell signaling,Human,Neurodevelopment

Search Keywords :

Barde, Y.-A., Mature brain-derived neurotrophic factor, Fish/Human, MIgG1, mammal/human/mouse/rat, BDNF, , AB_2617198, monoclonal, Cell signaling/Human/Neurodevelopment, Western Blot/ELISA

Antigen Molecular Weight :

Apparent: 14 kDa

Gene :

BDNF

Uniprot ID :

P23560

Antibody Registry ID :

AB_2617198

Synonyms (Alternate Clone Names) :

mAb#1

Immunogen :

Fish and Human BDNF

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

X63.Ag 8.635

Epitope Mapped :

Recommended Applications :

ELISA,Western Blot

Immunoblotting (legacy) :

yes, but poorly

Western Blot Pubmed IDs :

10217270

ELISA Pubmed IDs :

10217270 18204444 23844236 21935677

Additional Information :

For ELISA, BDNF-#1 is often used in combination with BDNF-#9 (a different clone from the same fusion; available in the DSHB collection) that recognizes a different epitope on BDNF.

DSHB Growth Medium :

RPMI

References (legacy) :

J. Neurochem. 72, 1930-1938.; Nat. Neurosci. 11, 131-133.; J. Cell Biol. 196, 775-788.

more info or order :

Developmental Studies Hybridoma Bank product webpage