product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Myosin heavy chain (slow, alpha- and beta-)
catalog :
BA-F8
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
BA-F8
reactivity :
human, mouse, rat, bovine, swine
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
citations: 51
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; loading ...; fig 4b
Vang P, Vasdev A, Zhan W, Gransee H, Sieck G, Mantilla C. Diaphragm muscle sarcopenia into very old age in mice. Physiol Rep. 2020;8:e14305 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:50; loading ...; fig 4b
Bergmeister K, Aman M, Muceli S, Vujaklija I, Manzano Szalai K, Unger E, et al. Peripheral nerve transfers change target muscle structure and function. Sci Adv. 2019;5:eaau2956 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 3c
Honda M, Hidaka K, Fukada S, Sugawa R, Shirai M, Ikawa M, et al. Vestigial-like 2 contributes to normal muscle fiber type distribution in mice. Sci Rep. 2017;7:7168 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 3b
  • western blot; mouse; loading ...; fig 3c
Koh J, Hancock C, Terada S, Higashida K, Holloszy J, Han D. PPARβ Is Essential for Maintaining Normal Levels of PGC-1α and Mitochondria and for the Increase in Muscle Mitochondria Induced by Exercise. Cell Metab. 2017;25:1176-1185.e5 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig 1g
Lee C, Hanna A, Wang H, Dagnino Acosta A, Joshi A, Knoblauch M, et al. A chemical chaperone improves muscle function in mice with a RyR1 mutation. Nat Commun. 2017;8:14659 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 3b
Fajardo V, Gamu D, Mitchell A, Bloemberg D, Bombardier E, Chambers P, et al. Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice. PLoS ONE. 2017;12:e0173708 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:25; loading ...; fig 3c
Morrow R, Picard M, Derbeneva O, Leipzig J, McManus M, Gouspillou G, et al. Mitochondrial energy deficiency leads to hyperproliferation of skeletal muscle mitochondria and enhanced insulin sensitivity. Proc Natl Acad Sci U S A. 2017;114:2705-2710 pubmed publisher
  • immunohistochemistry; human; 1:25; loading ...
St Jean Pelletier F, Pion C, Leduc Gaudet J, Sgarioto N, Zovilé I, Barbat Artigas S, et al. The impact of ageing, physical activity, and pre-frailty on skeletal muscle phenotype, mitochondrial content, and intramyocellular lipids in men. J Cachexia Sarcopenia Muscle. 2017;8:213-228 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:25; loading ...; fig 4a
Spendiff S, Vuda M, Gouspillou G, Aare S, Pérez A, Morais J, et al. Denervation drives mitochondrial dysfunction in skeletal muscle of octogenarians. J Physiol. 2016;594:7361-7379 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:25; fig 1
Aare S, Spendiff S, Vuda M, Elkrief D, Pérez A, Wu Q, et al. Failed reinnervation in aging skeletal muscle. Skelet Muscle. 2016;6:29 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 2g
Coleman S, Rebalka I, D Souza D, Deodhare N, Desjardins E, Hawke T. Myostatin inhibition therapy for insulin-deficient type 1 diabetes. Sci Rep. 2016;6:32495 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 4a
Woodall B, Woodall M, Luongo T, Grisanti L, Tilley D, Elrod J, et al. Skeletal Muscle-specific G Protein-coupled Receptor Kinase 2 Ablation Alters Isolated Skeletal Muscle Mechanics and Enhances Clenbuterol-stimulated Hypertrophy. J Biol Chem. 2016;291:21913-21924 pubmed
  • immunohistochemistry; mouse; fig 2
Fajardo V, Smith I, Bombardier E, Chambers P, Quadrilatero J, Tupling A. Diaphragm assessment in mice overexpressing phospholamban in slow-twitch type I muscle fibers. Brain Behav. 2016;6:e00470 pubmed publisher
  • western blot; swine; 1:80; loading ...; fig 1b
Spanos D, Tørngren M, Christensen M, Baron C. Effect of oxygen level on the oxidative stability of two different retail pork products stored using modified atmosphere packaging (MAP). Meat Sci. 2016;113:162-9 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:25; fig 1b
Power G, Minozzo F, Spendiff S, Filion M, Konokhova Y, Purves Smith M, et al. Reduction in single muscle fiber rate of force development with aging is not attenuated in world class older masters athletes. Am J Physiol Cell Physiol. 2016;310:C318-27 pubmed publisher
  • immunohistochemistry; mouse; fig 1
Ebert S, Dyle M, Bullard S, Dierdorff J, Murry D, Fox D, et al. Identification and Small Molecule Inhibition of an Activating Transcription Factor 4 (ATF4)-dependent Pathway to Age-related Skeletal Muscle Weakness and Atrophy. J Biol Chem. 2015;290:25497-511 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 6
Giordano C, Lemaire C, Li T, Kimoff R, Petrof B. Autophagy-associated atrophy and metabolic remodeling of the mouse diaphragm after short-term intermittent hypoxia. PLoS ONE. 2015;10:e0131068 pubmed publisher
  • immunocytochemistry; mouse
Fajardo V, Bombardier E, McMillan E, TRAN K, Wadsworth B, Gamu D, et al. Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype. Dis Model Mech. 2015;8:999-1009 pubmed publisher
  • immunohistochemistry - frozen section; swine; 1:50
Clark D, Clark D, Beever J, Dilger A. Increased prenatal IGF2 expression due to the porcine intron3-G3072A mutation may be responsible for increased muscle mass. J Anim Sci. 2015;93:2546-58 pubmed publisher
  • western blot; human; fig 1
Bollinger L, Powell J, Houmard J, Witczak C, Brault J. Skeletal muscle myotubes in severe obesity exhibit altered ubiquitin-proteasome and autophagic/lysosomal proteolytic flux. Obesity (Silver Spring). 2015;23:1185-93 pubmed publisher
  • immunohistochemistry; human; 1:25
  • immunohistochemistry; mouse; 1:25
  • immunohistochemistry; rat; 1:25
Gouspillou G, Sgarioto N, Norris B, Barbat Artigas S, Aubertin Leheudre M, Morais J, et al. The relationship between muscle fiber type-specific PGC-1α content and mitochondrial content varies between rodent models and humans. PLoS ONE. 2014;9:e103044 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:25
Gouspillou G, Sgarioto N, Kapchinsky S, Purves Smith F, Norris B, Pion C, et al. Increased sensitivity to mitochondrial permeability transition and myonuclear translocation of endonuclease G in atrophied muscle of physically active older humans. FASEB J. 2014;28:1621-33 pubmed publisher
  • immunohistochemistry - frozen section; mouse
Barton E, Park S, James J, Makarewich C, Philippou A, Eletto D, et al. Deletion of muscle GRP94 impairs both muscle and body growth by inhibiting local IGF production. FASEB J. 2012;26:3691-702 pubmed publisher
Carroll K, Bazyler C, Bernards J, Taber C, Stuart C, DeWeese B, et al. Skeletal Muscle Fiber Adaptations Following Resistance Training Using Repetition Maximums or Relative Intensity. Sports (Basel). 2019;7: pubmed publisher
Sonjak V, Jacob K, Morais J, Rivera Zengotita M, Spendiff S, Spake C, et al. Fidelity of muscle fibre reinnervation modulates ageing muscle impact in elderly women. J Physiol. 2019;597:5009-5023 pubmed publisher
Hunt L, Stover J, Haugen B, Shaw T, Li Y, Pagala V, et al. A Key Role for the Ubiquitin Ligase UBR4 in Myofiber Hypertrophy in Drosophila and Mice. Cell Rep. 2019;28:1268-1281.e6 pubmed publisher
Wang C, Arrington J, Ratliff A, Chen J, Horton H, Nie Y, et al. Methyltransferase-like 21c methylates and stabilizes the heat shock protein Hspa8 in type I myofibers in mice. J Biol Chem. 2019;294:13718-13728 pubmed publisher
Suzuki J. Effects of exercise training with short-duration intermittent hypoxia on endurance performance and muscle metabolism in well-trained mice. Physiol Rep. 2019;7:e14182 pubmed publisher
Iso T, Haruyama H, Sunaga H, Matsui M, Matsui H, Tanaka R, et al. Exercise endurance capacity is markedly reduced due to impaired energy homeostasis during prolonged fasting in FABP4/5 deficient mice. BMC Physiol. 2019;19:1 pubmed publisher
Cullins M, Krekeler B, Connor N. Differential impact of tongue exercise on intrinsic lingual muscles. Laryngoscope. 2018;128:2245-2251 pubmed publisher
García Liñeiro J, Graziotti G, Rodríguez Menéndez J, Rios C, Affricano N, Victorica C. Structural and functional characteristics of the thoracolumbar multifidus muscle in horses. J Anat. 2017;230:398-406 pubmed publisher
De Smet S, Van Thienen R, Deldicque L, James R, Sale C, Bishop D, et al. Nitrate Intake Promotes Shift in Muscle Fiber Type Composition during Sprint Interval Training in Hypoxia. Front Physiol. 2016;7:233 pubmed publisher
Bergmeister K, Gröger M, Aman M, Willensdorfer A, Manzano Szalai K, Salminger S, et al. Automated muscle fiber type population analysis with ImageJ of whole rat muscles using rapid myosin heavy chain immunohistochemistry. Muscle Nerve. 2016;54:292-9 pubmed publisher
Thomas M, Trajcevski K, Coleman S, Jiang M, Di Michele J, O Neill H, et al. Early oxidative shifts in mouse skeletal muscle morphology with high-fat diet consumption do not lead to functional improvements. Physiol Rep. 2014;2: pubmed publisher
Dyle M, Ebert S, Cook D, Kunkel S, Fox D, Bongers K, et al. Systems-based discovery of tomatidine as a natural small molecule inhibitor of skeletal muscle atrophy. J Biol Chem. 2014;289:14913-24 pubmed publisher
Fajardo V, Bombardier E, Vigna C, Devji T, Bloemberg D, Gamu D, et al. Co-expression of SERCA isoforms, phospholamban and sarcolipin in human skeletal muscle fibers. PLoS ONE. 2013;8:e84304 pubmed publisher
Bombardier E, Vigna C, Bloemberg D, Quadrilatero J, Tiidus P, Tupling A. The role of estrogen receptor-? in estrogen-mediated regulation of basal and exercise-induced Hsp70 and Hsp27 expression in rat soleus. Can J Physiol Pharmacol. 2013;91:823-29 pubmed
Schwend T, Jin Z, Jiang K, Mitchell B, Jia J, Yang J. Stabilization of speckle-type POZ protein (Spop) by Daz interacting protein 1 (Dzip1) is essential for Gli turnover and the proper output of Hedgehog signaling. J Biol Chem. 2013;288:32809-20 pubmed publisher
O Connell R, Carberry J, O Halloran K. Sternohyoid and diaphragm muscle form and function during postnatal development in the rat. Exp Physiol. 2013;98:1386-400 pubmed publisher
Radzyukevich T, Neumann J, Rindler T, Oshiro N, Goldhamer D, Lingrel J, et al. Tissue-specific role of the Na,K-ATPase ?2 isozyme in skeletal muscle. J Biol Chem. 2013;288:1226-37 pubmed publisher
Graziotti G, Chamizo V, Ríos C, Acevedo L, Rodríguez Menéndez J, Victorica C, et al. Adaptive functional specialisation of architectural design and fibre type characteristics in agonist shoulder flexor muscles of the llama, Lama glama. J Anat. 2012;221:151-63 pubmed publisher
Bloemberg D, Quadrilatero J. Rapid determination of myosin heavy chain expression in rat, mouse, and human skeletal muscle using multicolor immunofluorescence analysis. PLoS ONE. 2012;7:e35273 pubmed publisher
Banfi S, Monti L, Acquati F, Tettamanti G, de Eguileor M, Grimaldi A. Muscle development and differentiation in the urodele Ambystoma mexicanum. Dev Growth Differ. 2012;54:489-502 pubmed publisher
Tupling A, Bombardier E, Gupta S, Hussain D, Vigna C, Bloemberg D, et al. Enhanced Ca2+ transport and muscle relaxation in skeletal muscle from sarcolipin-null mice. Am J Physiol Cell Physiol. 2011;301:C841-9 pubmed publisher
Graziotti G, Palencia P, Delhon G, Rivero J. Neuromuscular partitioning, architectural design, and myosin fiber types of the M. vastus lateralis of the llama (Lama glama). J Morphol. 2004;262:667-81 pubmed
Grimaldi A, Tettamanti G, Martin B, Gaffield W, Pownall M, Hughes S. Hedgehog regulation of superficial slow muscle fibres in Xenopus and the evolution of tetrapod trunk myogenesis. Development. 2004;131:3249-62 pubmed
Dunn S, Michel R. Coordinated expression of myosin heavy chain isoforms and metabolic enzymes within overloaded rat muscle fibers. Am J Physiol. 1997;273:C371-83 pubmed
Michel R, Parry D, Dunn S. Regulation of myosin heavy chain expression in adult rat hindlimb muscles during short-term paralysis: comparison of denervation and tetrodotoxin-induced neural inactivation. FEBS Lett. 1996;391:39-44 pubmed
Levin V, Tarasov N, Prokof eva I, Kozlova M, Sedova T. [Use of colloid solution of In113m in clinical practice]. Med Radiol (Mosk). 1973;18:25-31 pubmed
Borrione A, Zanellato A, Saggin L, Mazzoli M, Azzarello G, Sartore S. Neonatal myosin heavy chains are not expressed in Ni-induced rat rhabdomyosarcoma. Differentiation. 1988;38:49-59 pubmed
Jennings H, Gamian A, Ashton F. N-propionylated group B meningococcal polysaccharide mimics a unique epitope on group B Neisseria meningitidis. J Exp Med. 1987;165:1207-11 pubmed
product information
Internal ID :
5824
Name :
BA-F8
Depositor Name :
Schiaffino, S.
Depositor Institution :
University of Padova
Depositor Website :
http://www.vimm.it/research/group.php?PI=12
Date Deposited :
3/25/09
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Myosin heavy chain (slow, alpha- and beta-)
Antigen Species :
Bovine
Host Species :
mouse
Isotype :
MIgG2b
Isotype for catalog (legacy) :
IgG2b
Positive Tested Species Reactivity :
Axolotl,Human,Llama,Rodent,Xenopus
Species Tested (legacy) :
rat, mouse, sheep, human
Initial Publication Pubmed ID :
3181651
Depositor Notes (Special Instructions) :
Fusion: 1986. Recognizes type 1 fibers (alpha- and beta-slow MyHC) in skeletal muscle
Collections :
Cardiac development,Cell markers,Cytoskeleton,Human,Skeletal muscle,Xenopus
Search Keywords :
Schiaffino, Stefano, Myosin heavy chain (slow, alpha- and beta-), Bovine, MIgG2b, Human/Rodent/Llama/Axolotl/xenopus, MYH7, MyHC-slow, AB_10572253, monoclonal, Human/Cell markers/Cytoskeletal elements/Skeletal muscle/Xenopus/Cardiac development proteins, Immunohistochemistry/Immunofluorescence/Western Blot
Antigen Molecular Weight :
220kDa
Gene :
MYH7
Alternate Gene Name(s) :
MyHC-slow
Uniprot ID :
Q9BE39
Antibody Registry ID :
AB_10572253
Immunogen :
purified myosin from adult bovine atrium
Clonality :
Monoclonal
Myeloma Strain :
NS0
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
9277335 22625659 3181651 22519643 15201218 2435835 24072710 22519643 15376272 15376272 23709586 24303535 25121500 24719321 27861847 31365869 31368533
Immunofluorescence Pubmed IDs :
22530000 8706926 23192345 22519643 21697544 4789910 29243257 26788932 30866899 31346037 31328438 31346037 31373325
Western Blot Pubmed IDs :
3181651 22519643 24358354 22519643 31373325
Additional Information :
This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
DSHB Growth Medium :
Iscove's
References (legacy) :
Differentiation 38(1), 49-59.; Cell Tissue Res. 254(1), 59-68.; Asian-Aust. J. Anim. Sci. 24(1), 125-129.; J. Biol. Chem. 288(2), 1226-1237.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US