product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Myosin Heavy Chain Type I
catalog :
BA-D5
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
BA-D5
reactivity :
human, mouse, rat, dogs, bovine, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
citations: 52
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; loading ...
Silva Rojas R, Charles A, Djeddi S, Geny B, Laporte J, Böhm J. Pathophysiological Effects of Overactive STIM1 on Murine Muscle Function and Structure. Cells. 2021;10: pubmed publisher
  • immunohistochemistry; mouse; loading ...
Steinert N, Potts G, Wilson G, Klamen A, Lin K, Hermanson J, et al. Mapping of the contraction-induced phosphoproteome identifies TRIM28 as a significant regulator of skeletal muscle size and function. Cell Rep. 2021;34:108796 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; fig 2e
Seo J, Kang J, Kim Y, Jo Y, Kim J, Hann S, et al. Maintenance of type 2 glycolytic myofibers with age by Mib1-Actn3 axis. Nat Commun. 2021;12:1294 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 2 ug/ml; loading ...; fig s6a
Pereira J, Gerber J, Ghidinelli M, Gerber D, Tortola L, Ommer A, et al. Mice carrying an analogous heterozygous dynamin 2 K562E mutation that causes neuropathy in humans develop predominant characteristics of a primary myopathy. Hum Mol Genet. 2020;29:1253-1273 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:10; loading ...; fig 4a
Arc Chagnaud C, Py G, Fovet T, Roumanille R, Demangel R, Pagano A, et al. Evaluation of an Antioxidant and Anti-inflammatory Cocktail Against Human Hypoactivity-Induced Skeletal Muscle Deconditioning. Front Physiol. 2020;11:71 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 3f
Owen A, Patel S, Smith J, Balasuriya B, Mori S, Hawk G, et al. Chronic muscle weakness and mitochondrial dysfunction in the absence of sustained atrophy in a preclinical sepsis model. elife. 2019;8: pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 4a
Gallot Y, Bohnert K, Straughn A, Xiong G, Hindi S, Kumar A. PERK regulates skeletal muscle mass and contractile function in adult mice. FASEB J. 2019;33:1946-1962 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:40; loading ...; fig 4i
Krag T, Ruiz Ruiz C, Vissing J. Glycogen Synthesis in Glycogenin 1-Deficient Patients: A Role for Glycogenin 2 in Muscle. J Clin Endocrinol Metab. 2017;102:2690-2700 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 3
Shen C, Zhou J, Wang X, Yu X, Liang C, Liu B, et al. Angiotensin-II-induced Muscle Wasting is Mediated by 25-Hydroxycholesterol via GSK3? Signaling Pathway. EBioMedicine. 2017;16:238-250 pubmed publisher
  • immunohistochemistry - frozen section; human; loading ...; fig 7a
Fry C, Johnson D, Ireland M, Noehren B. ACL injury reduces satellite cell abundance and promotes fibrogenic cell expansion within skeletal muscle. J Orthop Res. 2017;35:1876-1885 pubmed publisher
  • immunohistochemistry; human; 1:75; loading ...; tbl 3
McKenzie A, D Lugos A, Saunders M, Gworek K, Luden N. Fiber Type-Specific Satellite Cell Content in Cyclists Following Heavy Training with Carbohydrate and Carbohydrate-Protein Supplementation. Front Physiol. 2016;7:550 pubmed
  • immunohistochemistry; mouse; 1:2
Southard S, Kim J, Low S, Tsika R, Lepper C. Myofiber-specific TEAD1 overexpression drives satellite cell hyperplasia and counters pathological effects of dystrophin deficiency. elife. 2016;5: pubmed publisher
  • immunohistochemistry; human; loading ...; fig 1a
White S, McDermott M, Sufit R, Kosmac K, Bugg A, Gonzalez Freire M, et al. Walking performance is positively correlated to calf muscle fiber size in peripheral artery disease subjects, but fibers show aberrant mitophagy: an observational study. J Transl Med. 2016;14:284 pubmed publisher
  • immunohistochemistry; human; 1:75; loading ...; fig 2d
Murach K, Walton R, Fry C, Michaelis S, Groshong J, Finlin B, et al. Cycle training modulates satellite cell and transcriptional responses to a bout of resistance exercise. Physiol Rep. 2016;4: pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 3d
Liu J, Liang X, Zhou D, Lai L, Xiao L, Liu L, et al. Coupling of mitochondrial function and skeletal muscle fiber type by a miR-499/Fnip1/AMPK circuit. EMBO Mol Med. 2016;8:1212-1228 pubmed publisher
  • immunohistochemistry; rat; 1:100; loading ...; fig 1d
Rui Y, Pan F, Mi J. Composition of Muscle Fiber Types in Rat Rotator Cuff Muscles. Anat Rec (Hoboken). 2016;299:1397-401 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 5
Riaz M, Raz Y, van Putten M, Paniagua Soriano G, Krom Y, Florea B, et al. PABPN1-Dependent mRNA Processing Induces Muscle Wasting. PLoS Genet. 2016;12:e1006031 pubmed publisher
  • immunohistochemistry - frozen section; rat; loading ...
Pannérec A, Springer M, Migliavacca E, Ireland A, Piasecki M, Karaz S, et al. A robust neuromuscular system protects rat and human skeletal muscle from sarcopenia. Aging (Albany NY). 2016;8:712-29 pubmed publisher
  • immunohistochemistry - frozen section; bovine; 1:10
Phelps K, Drouillard J, Silva M, Miranda L, Ebarb S, Van Bibber Krueger C, et al. Effect of extended postmortem aging and steak location on myofibrillar protein degradation and Warner-Bratzler shear force of beef M. semitendinosus steaks. J Anim Sci. 2016;94:412-23 pubmed publisher
  • immunohistochemistry - frozen section; human; loading ...; fig 4a
Arentson Lantz E, English K, Paddon Jones D, Fry C. Fourteen days of bed rest induces a decline in satellite cell content and robust atrophy of skeletal muscle fibers in middle-aged adults. J Appl Physiol (1985). 2016;120:965-75 pubmed publisher
  • immunohistochemistry; mouse; fig 4
Foltz S, Modi J, Melick G, Abousaud M, Luan J, Fortunato M, et al. Abnormal Skeletal Muscle Regeneration plus Mild Alterations in Mature Fiber Type Specification in Fktn-Deficient Dystroglycanopathy Muscular Dystrophy Mice. PLoS ONE. 2016;11:e0147049 pubmed publisher
  • immunohistochemistry; mouse; fig s5
Ohsawa Y, Takayama K, Nishimatsu S, Okada T, Fujino M, Fukai Y, et al. The Inhibitory Core of the Myostatin Prodomain: Its Interaction with Both Type I and II Membrane Receptors, and Potential to Treat Muscle Atrophy. PLoS ONE. 2015;10:e0133713 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 5
Tian L, Ding S, You Y, Li T, Liu Y, Wu X, et al. Leiomodin-3-deficient mice display nemaline myopathy with fast-myofiber atrophy. Dis Model Mech. 2015;8:635-41 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100
Fry C, Lee J, Mula J, Kirby T, Jackson J, Liu F, et al. Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. Nat Med. 2015;21:76-80 pubmed publisher
  • immunohistochemistry; human
Fry C, Noehren B, Mula J, Ubele M, Westgate P, Kern P, et al. Fibre type-specific satellite cell response to aerobic training in sedentary adults. J Physiol. 2014;592:2625-35 pubmed publisher
  • immunohistochemistry; human; 1:100
Hauerslev S, Sveen M, Vissing J, Krag T. Protein turnover and cellular stress in mildly and severely affected muscles from patients with limb girdle muscular dystrophy type 2I. PLoS ONE. 2013;8:e66929 pubmed publisher
Barnes T, Cadaret C, Beede K, Schmidt T, Petersen J, Yates D. Hypertrophic muscle growth and metabolic efficiency were impaired by chronic heat stress, improved by zilpaterol supplementation, and not affected by ractopamine supplementation in feedlot lambs1. J Anim Sci. 2019;97:4101-4113 pubmed publisher
Muyskens J, Foote D, Bigot N, Strycker L, Smolkowski K, Kirkpatrick T, et al. Cellular and morphological changes with EAA supplementation before and after total knee arthroplasty. J Appl Physiol (1985). 2019;127:531-545 pubmed publisher
Wang T, Xu Y, Yuan Y, Xu P, Zhang C, Li F, et al. Succinate induces skeletal muscle fiber remodeling via SUNCR1 signaling. EMBO Rep. 2019;20:e47892 pubmed publisher
Liu Z, Chen J, Chen Z. Muscle Fiber Type Changes in Lumbrical Muscles at Early Stages of Chronic Nerve Compression. Curr Med Sci. 2019;39:59-66 pubmed publisher
Yao X, Yu T, Xi F, Xu Y, Ma L, Pan X, et al. BAMBI shuttling between cytosol and membrane is required for skeletal muscle development and regeneration. Biochem Biophys Res Commun. 2018;: pubmed publisher
Ryan T, Yamaguchi D, Schmidt C, Zeczycki T, Shaikh S, Brophy P, et al. Extensive skeletal muscle cell mitochondriopathy distinguishes critical limb ischemia patients from claudicants. JCI Insight. 2018;3: pubmed publisher
Yalvac M, Amornvit J, Braganza C, Chen L, Hussain S, Shontz K, et al. Impaired regeneration in calpain-3 null muscle is associated with perturbations in mTORC1 signaling and defective mitochondrial biogenesis. Skelet Muscle. 2017;7:27 pubmed publisher
Murgia M, Toniolo L, Nagaraj N, Ciciliot S, Vindigni V, Schiaffino S, et al. Single Muscle Fiber Proteomics Reveals Fiber-Type-Specific Features of Human Muscle Aging. Cell Rep. 2017;19:2396-2409 pubmed publisher
Goh Q, Millay D. Requirement of myomaker-mediated stem cell fusion for skeletal muscle hypertrophy. elife. 2017;6: pubmed publisher
Pardes A, Freedman B, Fryhofer G, Salka N, Bhatt P, Soslowsky L. Males have Inferior Achilles Tendon Material Properties Compared to Females in a Rodent Model. Ann Biomed Eng. 2016;44:2901-2910 pubmed publisher
Gorski J, Huffman N, Vallejo J, Brotto L, Chittur S, Breggia A, et al. Deletion of Mbtps1 (Pcsk8, S1p, Ski-1) Gene in Osteocytes Stimulates Soleus Muscle Regeneration and Increased Size and Contractile Force with Age. J Biol Chem. 2016;291:4308-22 pubmed publisher
Stark D, Coffey N, Pancoast H, Arnold L, Walker J, Vallée J, et al. Ephrin-A3 promotes and maintains slow muscle fiber identity during postnatal development and reinnervation. J Cell Biol. 2015;211:1077-91 pubmed publisher
Hergenreder J, Legako J, Dinh T, Spivey K, Baggerman J, Broadway P, et al. Zinc Methionine Supplementation Impacts Gene and Protein Expression in Calf-Fed Holstein Steers with Minimal Impact on Feedlot Performance. Biol Trace Elem Res. 2016;171:315-327 pubmed publisher
Hyldahl R, Olson T, Welling T, Groscost L, Parcell A. Satellite cell activity is differentially affected by contraction mode in human muscle following a work-matched bout of exercise. Front Physiol. 2014;5:485 pubmed publisher
Braun T, Szumowski M, Levasseur P, Grossberg A, Zhu X, Agarwal A, et al. Muscle atrophy in response to cytotoxic chemotherapy is dependent on intact glucocorticoid signaling in skeletal muscle. PLoS ONE. 2014;9:e106489 pubmed publisher
Moore C, Crocker D, Fahlman A, Moore M, Willoughby D, Robbins K, et al. Ontogenetic changes in skeletal muscle fiber type, fiber diameter and myoglobin concentration in the Northern elephant seal (Mirounga angustirostris). Front Physiol. 2014;5:217 pubmed publisher
Malfatti E, Lehtokari V, Böhm J, De Winter J, Schäffer U, Estournet B, et al. Muscle histopathology in nebulin-related nemaline myopathy: ultrastrastructural findings correlated to disease severity and genotype. Acta Neuropathol Commun. 2014;2:44 pubmed publisher
Dyar K, Ciciliot S, Wright L, Biensø R, Tagliazucchi G, Patel V, et al. Muscle insulin sensitivity and glucose metabolism are controlled by the intrinsic muscle clock. Mol Metab. 2014;3:29-41 pubmed publisher
Bennett B, Mohamed J, Alway S. Effects of resveratrol on the recovery of muscle mass following disuse in the plantaris muscle of aged rats. PLoS ONE. 2013;8:e83518 pubmed publisher
Gangopadhyay S. Systemic administration of follistatin288 increases muscle mass and reduces fat accumulation in mice. Sci Rep. 2013;3:2441 pubmed publisher
Meyers J, Planamento J, Ebrom P, Krulewitz N, Wade E, Pownall M. Sulf1 modulates BMP signaling and is required for somite morphogenesis and development of the horizontal myoseptum. Dev Biol. 2013;378:107-21 pubmed publisher
Didier N, Hourde C, Amthor H, Marazzi G, Sassoon D. Loss of a single allele for Ku80 leads to progenitor dysfunction and accelerated aging in skeletal muscle. EMBO Mol Med. 2012;4:910-23 pubmed publisher
Smerdu V, Soukup T. Demonstration of myosin heavy chain isoforms in rat and humans: the specificity of seven available monoclonal antibodies used in immunohistochemical and immunoblotting methods. Eur J Histochem. 2008;52:179-90 pubmed
Acevedo L, Rivero J. New insights into skeletal muscle fibre types in the dog with particular focus towards hybrid myosin phenotypes. Cell Tissue Res. 2006;323:283-303 pubmed
Sharp S, Dingermann T, Schaack J, Sharp J, Burke D, DeRobertis E, et al. Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes. Nucleic Acids Res. 1983;11:8677-90 pubmed
Schiaffino S, Gorza L, Sartore S, Saggin L, Ausoni S, Vianello M, et al. Three myosin heavy chain isoforms in type 2 skeletal muscle fibres. J Muscle Res Cell Motil. 1989;10:197-205 pubmed
product information
Internal ID :
5818
Name :
BA-D5
Depositor Name :
Schiaffino, S.
Depositor Institution :
University of Padova
Depositor Website :
http://www.vimm.it/research/group.php?PI=7
Date Deposited :
3/25/09
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Myosin Heavy Chain Type I
Antigen Species :
Bovine
Host Species :
mouse
Isotype :
MIgG2b
Isotype for catalog (legacy) :
IgG2b
Positive Tested Species Reactivity :
Bovine,Canine,Fish,Goat,Guinea Pig,Horse,Human,Lamb,Llama,Mouse,Porcine,Rabbit,Rat,Zebrafish
Species Tested (legacy) :
rat, mouse, human, guinea pig, horse, llama, pig, goat, zebrafish
Initial Publication Pubmed ID :
2547831
Collections :
Skeletal muscle
Search Keywords :
schiaffino, Myosin Heavy Chain Type I, slow, alpha- and beta-, MYH7, monoclonal, IgG2b, Bovine,Canine,Fish,Goat,Guinea Pig,Horse,Human,Llama,Mouse,Porcine,Rat,Zebrafish, Immunofluorescence,Immunohistochemistry,Western Blot
Gene :
MYH7
Uniprot ID :
Q9BE39
Antibody Registry ID :
AB_2235587
Clonality :
Monoclonal
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
16163488 23583585 6561520 27687713 26719336 31343947 31410479
Immunofluorescence Pubmed IDs :
24567902 6561520 27150673 29241457 27150673 30385731 30580997 30868492 28614723 31318145
Western Blot Pubmed IDs :
18840559
Additional Information :
Muscle contraction. RRID:AB_2235587
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Muscle Res. Cell. Motil. 10(3), 197-205.; Cell Tissue Res. 323, 283-303.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US