product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
alkaline phosphatase, tissue-nonspecific
catalog :
B4-78
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
B4-78
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, FACS
more info or order :
citations: 14
Published Application/Species/Sample/Dilution | Reference |
---|---|
| |
| |
Muguruma Y, Yahata T, Miyatake H, Sato T, Uno T, Itoh J, et al. Reconstitution of the functional human hematopoietic microenvironment derived from human mesenchymal stem cells in the murine bone marrow compartment. Blood. 2006;107:1878-87 pubmed
| |
Eghbali Fatourechi G, Lamsam J, Fraser D, Nagel D, Riggs B, Khosla S. Circulating osteoblast-lineage cells in humans. N Engl J Med. 2005;352:1959-66 pubmed
| |
Sanchez de Medina F, Martinez Augustin O, Gonzalez R, Ballester I, Nieto A, Galvez J, et al. Induction of alkaline phosphatase in the inflamed intestine: a novel pharmacological target for inflammatory bowel disease. Biochem Pharmacol. 2004;68:2317-26 pubmed
| |
Dorheim M, Sullivan M, Dandapani V, Wu X, Hudson J, Segarini P, et al. Osteoblastic gene expression during adipogenesis in hematopoietic supporting murine bone marrow stromal cells. J Cell Physiol. 1993;154:317-28 pubmed
| |
Lawson G, Katzmann J, Kimlinger T, O Brien J. Isolation and preliminary characterization of a monoclonal antibody that interacts preferentially with the liver isoenzyme of human alkaline phosphatase. Clin Chem. 1985;31:381-5 pubmed
|
product information
Internal ID :
2809
Name :
B4-78
Depositor Name :
Katzmann, J.A.
Depositor Institution :
Mayo Clinic, Rochester
Date Deposited :
6/14/89
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
alkaline phosphatase, tissue-nonspecific
Antigen Species :
human
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Human,Mouse,Rat
Species Tested (legacy) :
human, mouse, rat
Initial Publication Pubmed ID :
3971557
Depositor Notes (Special Instructions) :
Specific for bone and liver ALP (does not react with intestinal or placental ALP; kidney ALP not tested).
Collections :
Enzymes,Human,Stem cell
Search Keywords :
Katzmann, J.A., alkaline phosphatase, tissue-nonspecific, human, MIgG1, kappa light chain, human/mouse/rat, ALPL, AP-TNAP, TNSALP, HOPS, AB_528075, monoclonal, Enzymes/Human/Stem cell, Immunohistochemistry/Immunofluorescence/Western Blot/FACS
Antigen Molecular Weight :
Predicted: 47, 49, and 53 kDa;Apparent: 75 kDa
Gene :
ALPL
Alternate Gene Name(s) :
AP-TNAP, TNSALP, HOPS
Uniprot ID :
P05186
Antibody Registry ID :
AB_528075
Immunogen :
Purified human bone alkaline phosphatase
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
NS1
Epitope Mapped :
No
Recommended Applications :
FACS,Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
15548378
Immunofluorescence Pubmed IDs :
8425912 15888696 16282345 18379017 19029403 27157261 20645320
Western Blot Pubmed IDs :
15548378 19695258 20645320 22569414 20564193
FACS Pubmed IDs :
15888696 18490653
DSHB Growth Medium :
RPMI
References (legacy) :
Clin. Chem. 31, 381-385.; J. Cell. Phys. 154, 317-328.; N. Engl. J. Med. 352, 1959-1966.; Blood 107(5), 1878-1887.; J. Histochem. Cytochem. 56(7), 629-638.; ; PNAS 105(20), 7281-7286.; J. Histochem. Cytochem. 57(3), 265-276.
more info or order :
company information
Developmental Studies Hybridoma Bank
review
product type
related products
browse more products
questions and comments