product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
alkaline phosphatase, tissue-nonspecific
catalog :
B4-78
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
B4-78
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, FACS
citations: 14
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:100; fig 3
Moshfegh C, Aires L, Kisielow M, Vogel V. A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways. Sci Rep. 2016;6:25104 pubmed publisher
  • immunocytochemistry; human; 1:1000
Faia Torres A, Goren T, Ihalainen T, Guimond Lischer S, Charnley M, Rottmar M, et al. Regulation of human mesenchymal stem cell osteogenesis by specific surface density of fibronectin: a gradient study. ACS Appl Mater Interfaces. 2015;7:2367-75 pubmed publisher
Martínez Moya P, Ortega González M, Gonzalez R, Anzola A, Ocón B, Hernández Chirlaque C, et al. Exogenous alkaline phosphatase treatment complements endogenous enzyme protection in colonic inflammation and reduces bacterial translocation in rats. Pharmacol Res. 2012;66:144-53 pubmed publisher
López Posadas R, Gonzalez R, Ballester I, Martínez Moya P, Romero Calvo I, Suárez M, et al. Tissue-nonspecific alkaline phosphatase is activated in enterocytes by oxidative stress via changes in glycosylation. Inflamm Bowel Dis. 2011;17:543-56 pubmed publisher
Speer M, Li X, Hiremath P, Giachelli C. Runx2/Cbfa1, but not loss of myocardin, is required for smooth muscle cell lineage reprogramming toward osteochondrogenesis. J Cell Biochem. 2010;110:935-47 pubmed publisher
Nigam V, Srivastava D. Notch1 represses osteogenic pathways in aortic valve cells. J Mol Cell Cardiol. 2009;47:828-34 pubmed publisher
de Oliva M, Maximiano W, de Castro L, da Silva P, Fernandes R, Ciancaglini P, et al. Treatment with a growth factor-protein mixture inhibits formation of mineralized nodules in osteogenic cell cultures grown on titanium. J Histochem Cytochem. 2009;57:265-76 pubmed publisher
Siddappa R, Martens A, Doorn J, Leusink A, Olivo C, Licht R, et al. cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo. Proc Natl Acad Sci U S A. 2008;105:7281-6 pubmed publisher
de Oliveira P, de Oliva M, Maximiano W, Sebastião K, Crippa G, Ciancaglini P, et al. Effects of a mixture of growth factors and proteins on the development of the osteogenic phenotype in human alveolar bone cell cultures. J Histochem Cytochem. 2008;56:629-38 pubmed publisher
Muguruma Y, Yahata T, Miyatake H, Sato T, Uno T, Itoh J, et al. Reconstitution of the functional human hematopoietic microenvironment derived from human mesenchymal stem cells in the murine bone marrow compartment. Blood. 2006;107:1878-87 pubmed
Eghbali Fatourechi G, Lamsam J, Fraser D, Nagel D, Riggs B, Khosla S. Circulating osteoblast-lineage cells in humans. N Engl J Med. 2005;352:1959-66 pubmed
Sanchez de Medina F, Martinez Augustin O, Gonzalez R, Ballester I, Nieto A, Galvez J, et al. Induction of alkaline phosphatase in the inflamed intestine: a novel pharmacological target for inflammatory bowel disease. Biochem Pharmacol. 2004;68:2317-26 pubmed
Dorheim M, Sullivan M, Dandapani V, Wu X, Hudson J, Segarini P, et al. Osteoblastic gene expression during adipogenesis in hematopoietic supporting murine bone marrow stromal cells. J Cell Physiol. 1993;154:317-28 pubmed
Lawson G, Katzmann J, Kimlinger T, O Brien J. Isolation and preliminary characterization of a monoclonal antibody that interacts preferentially with the liver isoenzyme of human alkaline phosphatase. Clin Chem. 1985;31:381-5 pubmed
product information
Internal ID :
2809
Name :
B4-78
Depositor Name :
Katzmann, J.A.
Depositor Institution :
Mayo Clinic, Rochester
Date Deposited :
6/14/89
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
alkaline phosphatase, tissue-nonspecific
Antigen Species :
human
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Human,Mouse,Rat
Species Tested (legacy) :
human, mouse, rat
Initial Publication Pubmed ID :
3971557
Depositor Notes (Special Instructions) :
Specific for bone and liver ALP (does not react with intestinal or placental ALP; kidney ALP not tested).
Collections :
Enzymes,Human,Stem cell
Search Keywords :
Katzmann, J.A., alkaline phosphatase, tissue-nonspecific, human, MIgG1, kappa light chain, human/mouse/rat, ALPL, AP-TNAP, TNSALP, HOPS, AB_528075, monoclonal, Enzymes/Human/Stem cell, Immunohistochemistry/Immunofluorescence/Western Blot/FACS
Antigen Molecular Weight :
Predicted: 47, 49, and 53 kDa;Apparent: 75 kDa
Gene :
ALPL
Alternate Gene Name(s) :
AP-TNAP, TNSALP, HOPS
Uniprot ID :
P05186
Antibody Registry ID :
AB_528075
Immunogen :
Purified human bone alkaline phosphatase
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
NS1
Epitope Mapped :
No
Recommended Applications :
FACS,Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
15548378
Immunofluorescence Pubmed IDs :
8425912 15888696 16282345 18379017 19029403 27157261 20645320
Western Blot Pubmed IDs :
15548378 19695258 20645320 22569414 20564193
FACS Pubmed IDs :
15888696 18490653
DSHB Growth Medium :
RPMI
References (legacy) :
Clin. Chem. 31, 381-385.; J. Cell. Phys. 154, 317-328.; N. Engl. J. Med. 352, 1959-1966.; Blood 107(5), 1878-1887.; J. Histochem. Cytochem. 56(7), 629-638.; ; PNAS 105(20), 7281-7286.; J. Histochem. Cytochem. 57(3), 265-276.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US