fibronectin, avian | Developmental Studies Hybridoma Bank B3/D6 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

fibronectin, avian

catalog :

B3/D6

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

B3/D6

reactivity :

tobacco hornworm, chicken

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 25

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; chicken; fig 1 western blot; tobacco hornworm; fig 3a	Boswell B, Korol A, West Mays J, Musil L. Dual function of TGF? in lens epithelial cell fate: implications for secondary cataract. Mol Biol Cell. 2017;28:907-921 pubmed publisher
immunohistochemistry; chicken; fig 6	Stanic K, Saldivia N, Förstera B, Torrejon M, Montecinos H, Caprile T. Expression Patterns of Extracellular Matrix Proteins during Posterior Commissure Development. Front Neuroanat. 2016;10:89 pubmed
immunohistochemistry; chicken; 1:5; loading ...; fig 5a	Nagy N, Barad C, Graham H, Hotta R, Cheng L, Fejszak N, et al. Sonic hedgehog controls enteric nervous system development by patterning the extracellular matrix. Development. 2016;143:264-75 pubmed publisher
	Iida H, Yang T, Yasugi S, Ishii Y. Temporal dissociation of developmental events in the chick eye under low temperature conditions. Dev Growth Differ. 2016;58:741-749 pubmed publisher
	Oltean A, Huang J, Beebe D, Taber L. Tissue growth constrained by extracellular matrix drives invagination during optic cup morphogenesis. Biomech Model Mechanobiol. 2016;15:1405-1421 pubmed
	Aleksandrova A, Czirok A, Kosa E, Galkin O, Cheuvront T, Rongish B. The endoderm and myocardium join forces to drive early heart tube assembly. Dev Biol. 2015;404:40-54 pubmed publisher
	Loganathan R, Little C, Joshi P, Filla M, Cheuvront T, Lansford R, et al. Identification of emergent motion compartments in the amniote embryo. Organogenesis. 2014;10:350-64 pubmed publisher
	Aleksandrova A, Rongish B, Little C, CzirÃ³k A. Active cell and ECM movements during development. Methods Mol Biol. 2015;1189:123-32 pubmed publisher
	Biro E, Kocsis K, Nagy N, Molnar D, Kabell S, Palya V, et al. Origin of the chicken splenic reticular cells influences the effect of the infectious bursal disease virus on the extracellular matrix. Avian Pathol. 2011;40:199-206 pubmed publisher
	Benazeraf B, Francois P, Baker R, Denans N, Little C, Pourquie O. A random cell motility gradient downstream of FGF controls elongation of an amniote embryo. Nature. 2010;466:248-52 pubmed publisher
	Nagy N, Mwizerwa O, Yaniv K, Carmel L, Pieretti Vanmarcke R, Weinstein B, et al. Endothelial cells promote migration and proliferation of enteric neural crest cells via beta1 integrin signaling. Dev Biol. 2009;330:263-72 pubmed publisher
	Zamir E, Rongish B, Little C. The ECM moves during primitive streak formation--computation of ECM versus cellular motion. PLoS Biol. 2008;6:e247 pubmed publisher
	Tomanek R, Hansen H, Dedkov E. Vascular patterning of the quail coronary system during development. Anat Rec A Discov Mol Cell Evol Biol. 2006;288:989-99 pubmed
	Gumati M, Magyar A, Nagy N, Kurucz E, Felföldi B, Oláh I. Extracellular matrix of different composition supports the various splenic compartments of guinea fowl ( Numida meleagris). Cell Tissue Res. 2003;312:333-43 pubmed
	Lwigale P, Thurmond J, Norton W, Spooner B, Wiens D. Simulated microgravity and hypergravity attenuate heart tissue development in explant culture. Cells Tissues Organs. 2000;167:171-83 pubmed
	Yablonka Reuveni Z, Christ B, Benson J. Transitions in cell organization and in expression of contractile and extracellular matrix proteins during development of chicken aortic smooth muscle: evidence for a complex spatial and temporal differentiation program. Anat Embryol (Berl). 1998;197:421-37 pubmed
	Wiens D. An alternative model for cell sheet migration on fibronectin during heart formation. J Theor Biol. 1996;179:33-9 pubmed
	Hilfer S, Randolph G. Immunolocalization of basal lamina components during development of chick otic and optic primordia. Anat Rec. 1993;235:443-52 pubmed
	Harrisson F, van Nassauw L, van Hoof J, Foidart J. Microinjection of antifibronectin antibodies in the chicken blastoderm: inhibition of mesoblast cell migration but not of cell ingression at the primitive streak. Anat Rec. 1993;236:685-96 pubmed
	Gardner J, Fambrough D. Fibronectin expression during myogenesis. J Cell Biol. 1983;96:474-85 pubmed
	Solursh M, Jensen K. The accumulation of basement membrane components during the onset of chondrogenesis and myogenesis in the chick wing bud. Development. 1988;104:41-9 pubmed
	Zanetti N, Dress V, Solursh M. Comparison between ectoderm-conditioned medium and fibronectin in their effects on chondrogenesis by limb bud mesenchymal cells. Dev Biol. 1990;139:383-95 pubmed
	Shinomura T, Jensen K, Yamagata M, Kimata K, Solursh M. The distribution of mesenchyme proteoglycan (PG-M) during wing bud outgrowth. Anat Embryol (Berl). 1990;181:227-33 pubmed
	Vandenburgh H, Swasdison S, Karlisch P. Computer-aided mechanogenesis of skeletal muscle organs from single cells in vitro. FASEB J. 1991;5:2860-7 pubmed
	Chuong C, Chen H. Enhanced expression of neural cell adhesion molecules and tenascin (cytotactin) during wound healing. Am J Pathol. 1991;138:427-40 pubmed

product information

Internal ID :

2695

Name :

B3/D6

Depositor Name :

Fambrough, D.M.

Depositor Institution :

Johns Hopkins School of Medicine

Depositor Website :

http://www.searlescholars.net/person/475

Date Deposited :

4/8/86

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

fibronectin, avian

Antigen Species :

chicken

Host Species :

mouse

Isotype :

MIgG2a, kappa light chain

Isotype for catalog (legacy) :

IgG2a

Positive Tested Species Reactivity :

Avian

Species Tested (legacy) :

specific for avian fibronectin

Initial Publication Pubmed ID :

6833367

Depositor Notes (Special Instructions) :

Specific for avian fibronectin. Day 11 chicken embryonic myoblasts have extracellular fibronectin fibrils 24 hours after plating. By day 6 of culture 50% of the myotubes have detectable fibronectin, 75% by day 10. There is some intracellular staining of myotubes.

Collections :

Cell adhesion,Cell signaling,Extracellular matrix proteins

Search Keywords :

Fambrough, D.M., fibronectin, avian, chicken, MIgG2a, kappa light chain, Avian, FN1, FN, AB_2105970, monoclonal, Cell adhesion/Extracellular matrix proteins/Cell signaling, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/ELISA

Antigen Molecular Weight :

Predicted: 270 kDa; Apparent: 230 kDa (multiple isoforms)

Gene :

FN1

Alternate Gene Name(s) :

Uniprot ID :

F1NJT3

Antibody Registry ID :

AB_2105970

Synonyms (Alternate Clone Names) :

B3D6

Immunogen :

Purified cellular fibronectin from tertiary chicken embryo fibroblasts

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/0

Epitope Mapped :

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

1916108 8733429 10971041 12756528 19345201 21500040

Immunofluorescence Pubmed IDs :

6833367 3253060 2186947 2186666 1704192 8430914 9682974 16892426 27733818 25952622 25245691 25482403 26984743 27921294

Western Blot Pubmed IDs :

6833367 2186947

Immunoprecipitation Pubmed IDs :

6833367

Pubmed IDs :

8379592

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 96, 474-485.; Development 104,41-49.; Dev. Biol. 139, 383-395.; Anat. Embryol. 181, 227-233.; Am. J. Pathol. 138(2), 427-432.; FASEB J. 5(13), 2860-2867.; J. Tiss. Cult. Meth. 14, 31-36.; Anat. Rec. 235, 443-452.; Anat. Rec. 236, 685-696.; J. Theor. Biol. 179, 33-39.; Anat. Embryol. 197, 421-437.; Cells Tissues Org. 167, 171-183.; Cell Tissue Res. 312, 333-343.; Anat. Rec. A 288A, 989-999.; Dev. Biol. 330(2), 263-272.; Avian Pathol. 40(2), 199-206.

more info or order :

Developmental Studies Hybridoma Bank product webpage