ecdysone receptor (EcR common) | Developmental Studies Hybridoma Bank Ag10.2(EcR common) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

ecdysone receptor (EcR common)

catalog :

Ag10.2(EcR common)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Ag10.2(EcR common)

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 20

Reference
Romani P, Gargiulo G, Cavaliere V. The ecdysone receptor signalling regulates microvilli formation in follicular epithelial cells. Cell Mol Life Sci. 2016;73:409-25 pubmed publisher
Kumar S, Chen D, Jang C, Nall A, Zheng X, Sehgal A. An ecdysone-responsive nuclear receptor regulates circadian rhythms in Drosophila. Nat Commun. 2014;5:5697 pubmed publisher
Sap K, Bezstarosti K, Dekkers D, van den Hout M, van Ijcken W, Rijkers E, et al. Global quantitative proteomics reveals novel factors in the ecdysone signaling pathway in Drosophila melanogaster. Proteomics. 2015;15:725-38 pubmed publisher
Wang J, Wang S, Li S. Sumoylation modulates 20-hydroxyecdysone signaling by maintaining USP protein levels in Drosophila. Insect Biochem Mol Biol. 2014;54:80-8 pubmed publisher
Li Y, Ma Q, Cherry C, MATUNIS E. Steroid signaling promotes stem cell maintenance in the Drosophila testis. Dev Biol. 2014;394:129-41 pubmed publisher
Santhanam A, Peng W, Yu Y, Sang T, Chen G, Meng T. Ecdysone-induced receptor tyrosine phosphatase PTP52F regulates Drosophila midgut histolysis by enhancement of autophagy and apoptosis. Mol Cell Biol. 2014;34:1594-606 pubmed publisher
Wang S, Wang J, Sun Y, Song Q, Li S. PKC-mediated USP phosphorylation at Ser35 modulates 20-hydroxyecdysone signaling in Drosophila. J Proteome Res. 2012;11:6187-96 pubmed publisher
Chawla G, Sokol N. Hormonal activation of let-7-C microRNAs via EcR is required for adult Drosophila melanogaster morphology and function. Development. 2012;139:1788-97 pubmed publisher
Schwedes C, Tulsiani S, Carney G. Ecdysone receptor expression and activity in adult Drosophila melanogaster. J Insect Physiol. 2011;57:899-907 pubmed publisher
König A, Yatsenko A, WEISS M, Shcherbata H. Ecdysteroids affect Drosophila ovarian stem cell niche formation and early germline differentiation. EMBO J. 2011;30:1549-62 pubmed publisher
van der Knaap J, Kozhevnikova E, Langenberg K, Moshkin Y, Verrijzer C. Biosynthetic enzyme GMP synthetase cooperates with ubiquitin-specific protease 7 in transcriptional regulation of ecdysteroid target genes. Mol Cell Biol. 2010;30:736-44 pubmed publisher
Azoitei A, Spindler Barth M. DNA affects ligand binding of the ecdysone receptor of Drosophila melanogaster. Mol Cell Endocrinol. 2009;303:91-9 pubmed publisher
Bernardi F, Romani P, Tzertzinis G, Gargiulo G, Cavaliere V. EcR-B1 and Usp nuclear hormone receptors regulate expression of the VM32E eggshell gene during Drosophila oogenesis. Dev Biol. 2009;328:541-51 pubmed publisher
Hackney J, Pucci C, Naes E, Dobens L. Ras signaling modulates activity of the ecdysone receptor EcR during cell migration in the Drosophila ovary. Dev Dyn. 2007;236:1213-26 pubmed
Arbeitman M, Hogness D. Molecular chaperones activate the Drosophila ecdysone receptor, an RXR heterodimer. Cell. 2000;101:67-77 pubmed
Buszczak M, Freeman M, Carlson J, Bender M, Cooley L, Segraves W. Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila. Development. 1999;126:4581-9 pubmed
Mao H, Kaufman W. Profile of the ecdysteroid hormone and its receptor in the salivary gland of the adult female tick, Amblyomma hebraeum. Insect Biochem Mol Biol. 1999;29:33-42 pubmed
Mao H, Kaufman W. DNA binding properties of the ecdysteroid receptor in the salivary gland of the female ixodid tick, Amblyomma hebraeum. Insect Biochem Mol Biol. 1998;28:947-57 pubmed
Antoniewski C, Mugat B, Delbac F, Lepesant J. Direct repeats bind the EcR/USP receptor and mediate ecdysteroid responses in Drosophila melanogaster. Mol Cell Biol. 1996;16:2977-86 pubmed
Talbot W, Swyryd E, Hogness D. Drosophila tissues with different metamorphic responses to ecdysone express different ecdysone receptor isoforms. Cell. 1993;73:1323-37 pubmed

product information

Internal ID :

826

Name :

Ag10.2(EcR common)

Depositor Name :

Thummel, C. / Hogness, D.

Depositor Institution :

University of Utah

Depositor Website :

http://thummel.genetics.utah.edu/

Date Deposited :

11/1/01

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

ecdysone receptor (EcR common)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Amblyomma hebraeum,Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

8324824

Collections :

Cell signaling,Drosophila antigens,Neurodevelopment

Search Keywords :

Thummel, C. / Hogness, D., ecdysone receptor (EcR common), Drosophila, MIgG1, kappa light chain, Drosophila/Amblyomma hebraeum, EcR, NR1H1, AB_10683832, monoclonal, Cell signaling/Drosophila antigens/Neurodevelopment, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/Gel Supershift

Antigen Molecular Weight :

Predicted: 91 kDa (EcR-A), 94 kDa (EcR-B1), 73 kDa (EcR-B2); Apparent; 105 kDa (EcR-A and EcR-B1) and 80 kDa (EcR-B2)

Gene :

EcR

Alternate Gene Name(s) :

NR1H1

Uniprot ID :

P34021

Antibody Registry ID :

AB_10683832

Immunogen :

TrpE-EcR-B1 fusion protein containing both isoform-specific and common segments of EcR-Bl (residues 68-878).

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 649-878

Epitope Map PubMed ID :

8324824

Recommended Applications :

Gel Supershift,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunohistochemistry Pubmed IDs :

8324824 21507325

Immunofluorescence Pubmed IDs :

8324824 10498692 17436275 25511299 26223269 21423150 25093968 22510985 19389369

Western Blot Pubmed IDs :

8324824 9887511 10070743 19995917 24550005 23136906 22510985 25240618

Immunoprecipitation Pubmed IDs :

25403936

Gel Supershift Pubmed IDs :

10778857 9887511 8649409 22510985

Pubmed IDs :

19428996

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 73, 1323-1337.

more info or order :

Developmental Studies Hybridoma Bank product webpage