Adaptin | Developmental Studies Hybridoma Bank APA2 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Adaptin

catalog :

APA2

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

APA2

reactivity :

Caenorhabditis elegans

application :

western blot, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 2

Reference
Cheng L, Li B, Wang Z. Level-Set Minimization of Potential Controlled Hadwiger Valuations for Molecular Solvation. J Comput Phys. 2010;229:8497-8510 pubmed
Hadwiger G, Dour S, Arur S, Fox P, Nonet M. A monoclonal antibody toolkit for C. elegans. PLoS ONE. 2010;5:e10161 pubmed publisher

product information

Internal ID :

6466

Name :

APA2

Depositor Name :

Nonet, M.L. / Hadwiger, G. / Dour, S.

Depositor Institution :

Washington University Medical School

Date Deposited :

2/16/10

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Adaptin

Antigen Species :

C. elegans

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

C. elegans

Species Tested (legacy) :

C. elegans

Initial Publication Pubmed ID :

20405020

Depositor Notes (Special Instructions) :

Works on C. elegans whole mounts. Works poorly on immunoblots.

Collections :

C. elegans

Search Keywords :

Nonet, M.L. / Hadwiger, G. / Dour, S., Adaptin, C. elegans, MIgG1, C. elegans, apa-2, , AB_10567130, monoclonal, C. elegans, Immunofluorescence/Western Blot

Antigen Molecular Weight :

Predicted: 104 kDa; Apparent: 102 and 250 kDa

Gene :

apa-2

Uniprot ID :

Q22601

Antibody Registry ID :

AB_10567130

Synonyms (Alternate Clone Names) :

11B11

Immunogen :

Recombinant His6-tagged APA-2 (C-terminal 308 aa) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

P3X63Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

C-terminal 308 aa

Epitope Map PubMed ID :

20405020

Recommended Applications :

Immunofluorescence,Western Blot

Immunoblotting (legacy) :

poor

Immunofluorescence Pubmed IDs :

20405020

Western Blot Pubmed IDs :

20405020

Additional Information :

The AdaPtin, Alpha chain (Clathrin associated complex), APA-2, is an adaptin for the alpha subunit of the adaptor protein complex 2 (AP2) that is involved in endocytosis.

DSHB Growth Medium :

Iscove's

References (legacy) :

Hadwiger, G., Dour, S., Arur, S., Fox, P., and Nonet, M.L. (2010) A monoclonal antibody toolkit for C. elegans. PLoS One. 5(4):e10161-10115.

more info or order :

Developmental Studies Hybridoma Bank product webpage