Osteonectin (SPARC) | Developmental Studies Hybridoma Bank AON-1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Osteonectin (SPARC)

catalog :

AON-1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AON-1

reactivity :

human, rat, dog, cow

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:5	Pautke C, Schieker M, Tischer T, Kolk A, Neth P, Mutschler W, et al. Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts. Anticancer Res. 2004;24:3743-8 pubmed
	Leblon C, Casey M, Fodor C, Zhang T, Zhang X, Jedlicka S. Correlation between in vitro expansion-related cell stiffening and differentiation potential of human mesenchymal stem cells. Differentiation. 2015;90:1-15 pubmed publisher
	Fedorovich N, Schuurman W, Wijnberg H, Prins H, van Weeren P, Malda J, et al. Biofabrication of osteochondral tissue equivalents by printing topologically defined, cell-laden hydrogel scaffolds. Tissue Eng Part C Methods. 2012;18:33-44 pubmed publisher
	Chen J, Yu Q, Wu B, Lin Z, Pavlos N, Xu J, et al. Autologous tenocyte therapy for experimental Achilles tendinopathy in a rabbit model. Tissue Eng Part A. 2011;17:2037-48 pubmed publisher
	Juhásová J, Juhás S, Klima J, Strnádel J, Holubova M, Motlik J. Osteogenic differentiation of miniature pig mesenchymal stem cells in 2D and 3D environment. Physiol Res. 2011;60:559-71 pubmed
	Reding T, Wagner U, Silva A, Sun L, Bain M, Kim S, et al. Inflammation-dependent expression of SPARC during development of chronic pancreatitis in WBN/Kob rats and a microarray gene expression analysis. Physiol Genomics. 2009;38:196-204 pubmed publisher
	Koelling S, Kruegel J, Irmer M, Path J, Sadowski B, Miro X, et al. Migratory chondrogenic progenitor cells from repair tissue during the later stages of human osteoarthritis. Cell Stem Cell. 2009;4:324-35 pubmed publisher
	Cole A, Hall B. The nature and significance of invertebrate cartilages revisited: distribution and histology of cartilage and cartilage-like tissues within the Metazoa. Zoology (Jena). 2004;107:261-73 pubmed
	Bampton E, Ma C, Tolkovsky A, Taylor J. Osteonectin is a Schwann cell-secreted factor that promotes retinal ganglion cell survival and process outgrowth. Eur J Neurosci. 2005;21:2611-23 pubmed
	Lieb E, Vogel T, Milz S, Dauner M, Schulz M. Effects of transforming growth factor beta1 on bonelike tissue formation in three-dimensional cell culture. II: Osteoblastic differentiation. Tissue Eng. 2004;10:1414-25 pubmed
	Loukopoulos P, O BRIEN T, Ghoddusi M, Mungall B, Robinson W. Characterisation of three novel canine osteosarcoma cell lines producing high levels of matrix metalloproteinases. Res Vet Sci. 2004;77:131-41 pubmed
	Johansson C, Roser K, Bolind P, Donath K, Albrektsson T. Bone-tissue formation and integration of titanium implants: an evaluation with newly developed enzyme and immunohistochemical techniques. Clin Implant Dent Relat Res. 1999;1:33-40 pubmed
	Roser K, Johansson C, Donath K, Albrektsson T. A new approach to demonstrate cellular activity in bone formation adjacent to implants. J Biomed Mater Res. 2000;51:280-91 pubmed
	Bürgi Saville M, Gerber H, Peter H, Paulsson M, Aeschlimann D, Glaser C, et al. Expression patterns of extracellular matrix components in native and cultured normal human thyroid tissue and in human toxic adenoma tissue. Thyroid. 1997;7:347-56 pubmed
	Bolander M, Robey P, Fisher L, Conn K, Prabhakar B, Termine J. Monoclonal antibodies against osteonectin show conservation of epitopes across species. Calcif Tissue Int. 1989;45:74-80 pubmed

product information

Internal ID :

761

Name :

AON-1

Depositor Name :

Termine, J.D.

Depositor Institution :

Bone Research Branch, NIDR, NIH

Date Deposited :

7/13/90

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Osteonectin (SPARC)

Antigen Species :

Bovine

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Acorn worm,Bovine,Canine,Human,Monkey,Pig,Rabbit,Rat

Species Tested (legacy) :

human, cow, monkey, pig, dog, rabbit, rat

Initial Publication Pubmed ID :

2476206

Depositor Notes (Special Instructions) :

The isotype of this monoclonal antibody was originally reported as IgG3 (PMID: 2476206). This antibody recognizes osteonectin (SPARC)-containing cartilages in invertebrate species. See PMID: 16351944.

Collections :

Cell adhesion,Extracellular matrix proteins,Human

Search Keywords :

Termine, J.D., Osteonectin (SPARC), Bovine, MIgG1, kappa light chain, Human/Bovine/Monkey/Pig/canine/Rabbit/Rat/Acorn worm, SPARC, , AB_528421, Monoclonal , Human/Extracellular Matrix proteins, Western Blot/Immunoprecipitation/Immunohistochemistry/ELISA/Immunofluorescence/FFPE

Antigen Molecular Weight :

42 kDa

Gene :

SPARC

Uniprot ID :

P13213

Antibody Registry ID :

AB_528421

Immunogen :

Purified bovine osteonectin (SPARC)

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

x63-AG8.653

Epitope Mapped :

Epitope Location or Sequence :

The epitope bound by AON-1 is cryptic and often requires tissue pretreatment for exposure. See PMID: 19435834 & 2476206.

Recommended Applications :

ELISA,FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

10825228 11359309 2476206 15196903 19435834 21495863 21854293 15588401 31402634

Immunofluorescence Pubmed IDs :

9226202 2476206 21401296 9226202 19341622 26381795

Western Blot Pubmed IDs :

2476206 15926910

Immunoprecipitation Pubmed IDs :

2476206

ELISA Pubmed IDs :

2476206

FFPE Pubmed IDs :

16351944 31402634

Additional Information :

RRID:AB_528421

DSHB Growth Medium :

Iscove's

References (legacy) :

Calcif. Tiss. Int. 45, 74-80.; Thyroid 7(3), 347-356.; Clin. Imp. Dent. Rel. Res. 1(1), 33-40.; J. Biomed. Mater. Res. 51, 280-291.; Res. Vet. Sci. 77, 131-141.; Eur. J. Neurosci. 21, 2611-2623.; Physiol. Genomics 38, 196-204.

more info or order :

Developmental Studies Hybridoma Bank product webpage