product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
avian myoblastosis virus, p19^(gag)
catalog :
AMV-3C2
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
AMV-3C2
reactivity :
chicken, Rous sarcoma virus
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
citations: 50
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; chicken; 1:50; fig 9
Holzmann J, Hennchen M, Rohrer H. Prox1 identifies proliferating neuroblasts and nascent neurons during neurogenesis in sympathetic ganglia. Dev Neurobiol. 2015;75:1352-67 pubmed publisher
Santhakumar D, Rohaim M, Hussein H, Hawes P, Ferreira H, Behboudi S, et al. Chicken Interferon-induced Protein with Tetratricopeptide Repeats 5 Antagonizes Replication of RNA Viruses. Sci Rep. 2018;8:6794 pubmed publisher
Munnamalai V, Sienknecht U, Duncan R, Scott M, Thawani A, Fantetti K, et al. Wnt9a Can Influence Cell Fates and Neural Connectivity across the Radial Axis of the Developing Cochlea. J Neurosci. 2017;37:8975-8988 pubmed publisher
Nerurkar N, Mahadevan L, Tabin C. BMP signaling controls buckling forces to modulate looping morphogenesis of the gut. Proc Natl Acad Sci U S A. 2017;114:2277-2282 pubmed publisher
Koszinowski S, Boerries M, Busch H, Krieglstein K. RARβ regulates neuronal cell death and differentiation in the avian ciliary ganglion. Dev Neurobiol. 2015;75:1204-18 pubmed publisher
Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, et al. Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways. PLoS ONE. 2014;9:e104937 pubmed publisher
Ng C, Wu P, Fan W, Yan J, Chen C, Lai Y, et al. Genomic organization, transcriptomic analysis, and functional characterization of avian α- and β-keratins in diverse feather forms. Genome Biol Evol. 2014;6:2258-73 pubmed publisher
Zhu J, Luz Madrigal A, Haynes T, Zavada J, Burke A, Del Rio Tsonis K. ?-Catenin inactivation is a pre-requisite for chick retina regeneration. PLoS ONE. 2014;9:e101748 pubmed publisher
Li A, Chen M, Jiang T, Wu P, Nie Q, Widelitz R, et al. Shaping organs by a wingless-int/Notch/nonmuscle myosin module which orients feather bud elongation. Proc Natl Acad Sci U S A. 2013;110:E1452-61 pubmed publisher
Chen J, Zahid S, Shilts M, Weaver S, Leskowitz R, Habbsa S, et al. Hoxa-5 acts in segmented somites to regulate cervical vertebral morphology. Mech Dev. 2013;130:226-40 pubmed publisher
Ng C, Wu P, Foley J, Foley A, McDonald M, Juan W, et al. The chicken frizzle feather is due to an ?-keratin (KRT75) mutation that causes a defective rachis. PLoS Genet. 2012;8:e1002748 pubmed publisher
Tassew N, Charish J, Seidah N, Monnier P. SKI-1 and Furin generate multiple RGMa fragments that regulate axonal growth. Dev Cell. 2012;22:391-402 pubmed publisher
Kowalik L, Hudspeth A. A search for factors specifying tonotopy implicates DNER in hair-cell development in the chick's cochlea. Dev Biol. 2011;354:221-31 pubmed publisher
Mwizerwa O, Das P, Nagy N, Akbareian S, Mably J, Goldstein A. Gdnf is mitogenic, neurotrophic, and chemoattractive to enteric neural crest cells in the embryonic colon. Dev Dyn. 2011;240:1402-11 pubmed publisher
Sienknecht U, Anderson B, Parodi R, Fantetti K, Fekete D. Non-cell-autonomous planar cell polarity propagation in the auditory sensory epithelium of vertebrates. Dev Biol. 2011;352:27-39 pubmed publisher
McNally M, Wahlin K, Canto Soler M. Endogenous expression of ASLV viral proteins in specific pathogen free chicken embryos: relevance for the developmental biology research field. BMC Dev Biol. 2010;10:106 pubmed publisher
Kermer V, Ritter M, Albuquerque B, Leib C, Stanke M, Zimmermann H. Knockdown of tissue nonspecific alkaline phosphatase impairs neural stem cell proliferation and differentiation. Neurosci Lett. 2010;485:208-11 pubmed publisher
Fuchs A, Inthal A, Herrmann D, Cheng S, Nakatomi M, Peters H, et al. Regulation of Tbx22 during facial and palatal development. Dev Dyn. 2010;239:2860-74 pubmed publisher
Krawchuk D, Weiner S, Chen Y, Lu B, Costantini F, Behringer R, et al. Twist1 activity thresholds define multiple functions in limb development. Dev Biol. 2010;347:133-46 pubmed publisher
Suerth J, Maetzig T, Galla M, Baum C, Schambach A. Self-inactivating alpharetroviral vectors with a split-packaging design. J Virol. 2010;84:6626-35 pubmed publisher
Satoh A, Makanae A, Wada N. The apical ectodermal ridge (AER) can be re-induced by wounding, wnt-2b, and fgf-10 in the chicken limb bud. Dev Biol. 2010;342:157-68 pubmed publisher
Kothlow S, Schenk Weibhauser K, Ratcliffe M, Kaspers B. Prolonged effect of BAFF on chicken B cell development revealed by RCAS retroviral gene transfer in vivo. Mol Immunol. 2010;47:1619-28 pubmed publisher
Morillo S, Escoll P, de la Hera A, Frade J. Somatic tetraploidy in specific chick retinal ganglion cells induced by nerve growth factor. Proc Natl Acad Sci U S A. 2010;107:109-14 pubmed publisher
Takimoto A, Nishizaki Y, Hiraki Y, Shukunami C. Differential actions of VEGF-A isoforms on perichondrial angiogenesis during endochondral bone formation. Dev Biol. 2009;332:196-211 pubmed publisher
Mao J, McGlinn E, Huang P, Tabin C, McMahon A. Fgf-dependent Etv4/5 activity is required for posterior restriction of Sonic Hedgehog and promoting outgrowth of the vertebrate limb. Dev Cell. 2009;16:600-6 pubmed publisher
Tassew N, Charish J, Chestopalova L, Monnier P. Sustained in vivo inhibition of protein domains using single-chain Fv recombinant antibodies and its application to dissect RGMa activity on axonal outgrowth. J Neurosci. 2009;29:1126-31 pubmed publisher
Hong S, Lardaro T, Oh M, Huh Y, Ding Y, Kang U, et al. Regulation of the noradrenaline neurotransmitter phenotype by the transcription factor AP-2beta. J Biol Chem. 2008;283:16860-7 pubmed publisher
Havens B, Velonis D, Kronenberg M, Lichtler A, Oliver B, Mina M. Roles of FGFR3 during morphogenesis of Meckel's cartilage and mandibular bones. Dev Biol. 2008;316:336-49 pubmed publisher
Li H, Marijanovic I, Kronenberg M, Erceg I, Stover M, Velonis D, et al. Expression and function of Dlx genes in the osteoblast lineage. Dev Biol. 2008;316:458-70 pubmed publisher
Hruska M, Nishi R. Cell-autonomous inhibition of alpha 7-containing nicotinic acetylcholine receptors prevents death of parasympathetic neurons during development. J Neurosci. 2007;27:11501-9 pubmed
Spence J, Madhavan M, Aycinena J, Del Rio Tsonis K. Retina regeneration in the chick embryo is not induced by spontaneous Mitf downregulation but requires FGF/FGFR/MEK/Erk dependent upregulation of Pax6. Mol Vis. 2007;13:57-65 pubmed
Hong S, Huh Y, Chae H, Hong S, Lardaro T, Kim K. GATA-3 regulates the transcriptional activity of tyrosine hydroxylase by interacting with CREB. J Neurochem. 2006;98:773-81 pubmed
Moreira E, Adler R. Effects of follistatin overexpression on cell differentiation in the chick embryo retina. Dev Biol. 2006;298:272-84 pubmed
Matsunaga E, Nakamura H, Chedotal A. Repulsive guidance molecule plays multiple roles in neuronal differentiation and axon guidance. J Neurosci. 2006;26:6082-8 pubmed
Simard A, Di Pietro E, Young C, Plaza S, Ryan A. Alterations in heart looping induced by overexpression of the tight junction protein Claudin-1 are dependent on its C-terminal cytoplasmic tail. Mech Dev. 2006;123:210-27 pubmed
Harpavat S, Cepko C. RCAS-RNAi: a loss-of-function method for the developing chick retina. BMC Dev Biol. 2006;6:2 pubmed
Lee E, Linial M. Deletion of a Cys-His motif from the Alpharetrovirus nucleocapsid domain reveals late domain mutant-like budding defects. Virology. 2006;347:226-33 pubmed
Sato N, Sakuma C, Sato Y, Gould T, Oppenheim R, Yaginuma H. Distinct susceptibility of developing neurons to death following Bax overexpression in the chicken embryo. Cell Death Differ. 2006;13:435-45 pubmed
Harris M, Williamson S, Fallon J, Meinhardt H, Prum R. Molecular evidence for an activator-inhibitor mechanism in development of embryonic feather branching. Proc Natl Acad Sci U S A. 2005;102:11734-9 pubmed
Hornbruch A, Ma G, Ballermann M, Tumova K, Liu D, Cairine Logan C. A BMP-mediated transcriptional cascade involving Cash1 and Tlx-3 specifies first-order relay sensory neurons in the developing hindbrain. Mech Dev. 2005;122:900-13 pubmed
Huillard E, Laugier D, Marx M. Defects in chicken neuroretina misexpressing the BMP antagonist Drm/Gremlin. Dev Biol. 2005;283:335-44 pubmed
Person A, Garriock R, Krieg P, Runyan R, Klewer S. Frzb modulates Wnt-9a-mediated beta-catenin signaling during avian atrioventricular cardiac cushion development. Dev Biol. 2005;278:35-48 pubmed
Leu S, Jacques S, Wingerd K, Hikita S, Tolhurst E, Pring J, et al. Integrin alpha4beta1 function is required for cell survival in developing retina. Dev Biol. 2004;276:416-30 pubmed
Hornik C, Krishan K, Yusuf F, Scaal M, Brand Saberi B. cDermo-1 misexpression induces dense dermis, feathers, and scales. Dev Biol. 2005;277:42-50 pubmed
Spence J, Madhavan M, Ewing J, Jones D, Lehman B, Del Rio Tsonis K. The hedgehog pathway is a modulator of retina regeneration. Development. 2004;131:4607-21 pubmed
Brent A, Tabin C. FGF acts directly on the somitic tendon progenitors through the Ets transcription factors Pea3 and Erm to regulate scleraxis expression. Development. 2004;131:3885-96 pubmed
Fischer A, Wang S, Reh T. NeuroD induces the expression of visinin and calretinin by proliferating cells derived from toxin-damaged chicken retina. Dev Dyn. 2004;229:555-63 pubmed
Suksaweang S, Lin C, Jiang T, Hughes M, Widelitz R, Chuong C. Morphogenesis of chicken liver: identification of localized growth zones and the role of beta-catenin/Wnt in size regulation. Dev Biol. 2004;266:109-22 pubmed
Liu J, Laufer E, Jessell T. Assigning the positional identity of spinal motor neurons: rostrocaudal patterning of Hox-c expression by FGFs, Gdf11, and retinoids. Neuron. 2001;32:997-1012 pubmed
Potts W, Olsen M, Boettiger D, Vogt V. Epitope mapping of monoclonal antibodies to gag protein p19 of avian sarcoma and leukaemia viruses. J Gen Virol. 1987;68 ( Pt 12):3177-82 pubmed
product information
Internal ID :
1322
Name :
AMV-3C2
Depositor Name :
Boettiger, D.
Depositor Institution :
University of Pennsylvania
Date Deposited :
5/5/94
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
avian myoblastosis virus, p19^(gag)
Antigen Species :
Avian myoblastosis virus (AMV)
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Avian myoblastosis virus (AMV)
Initial Publication Pubmed ID :
2447226
Depositor Notes (Special Instructions) :
This antibody identifies cells infected with all strains of the avian sarcoma/leukemia virus complex and can also be used to identify cells harboring Rous sarcoma or avian leukemia virus-derived vectors. Gag-Pro polyprotein is cleaved into protein fragments; p19 represents the first 155 amino acids of the Gag-Pro polyprotein.
Collections :
Epitope tags,Microbe antibodies,Protein tags
Search Keywords :
Boettiger, David, avian myoblastosis virus, p19^(gag), Avian myoblastosis virus (AMV), MIgG1, Avian Myeloblastosis Virus, gag-pro, , AB_528098, monoclonal
Antigen Molecular Weight :
Predicted: 16.5kDa; Apparent: 19kDa
Gene :
gag-pro
Uniprot ID :
P03322
Antibody Registry ID :
AB_528098
Synonyms (Alternate Clone Names) :
3C2
Immunogen :
Avian Myeloblastosis Virus purified from infected chickens.
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
SP2/0-Ag14
Epitope Mapped :
Yes
Epitope Location or Sequence :
N-terminal a.a.1-51 near a.a. 40.
Epitope Map PubMed ID :
2447226
Recommended Applications :
FFPE,Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
18339367 20347761 20149459 25170755 22829773 20845426 15922575 15649459 15907833 16087884 14729482 16426460
Immunofluorescence Pubmed IDs :
19464280 16872597 20955591 21255565 25003522 23576731 19176821 23462683 16151456 15342484 15253939 14991711 11754833 21497156
Western Blot Pubmed IDs :
2447226 20410274
FFPE Pubmed IDs :
25152353 22829773 20845426
Pubmed IDs :
17959793 20849921 16893419 20732316 16738252 18280462 18424435 16406458 20018664 16500087 15572138 15581875 19386268 22340500
Depositor Growth Medium :
Iscove's
Additional Information :
Abdominal-b has two isoforms, M and R; isoform R lacks the amino terminal 223 aa portion of isoform M. Both M and R isoforms are recognized by mAb 1A2E9.
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Gen. Virol. 68, 3177-3182.; Dev. Biol. 233, 271-290.; Neuron 32, 997-1012.; Mol. Immunol. 47, 1619-1628.; Dev. Biol. 354, 221-231.; Dev. Dyn. 240, 1402-1411.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US