product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Vimentin
catalog :
AMF-17b
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
AMF-17b
reactivity :
human, dog, chicken, cow, pig
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 35
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 1b
Fenton S, Hutchens K, Denning M. Targeting Fyn in Ras-transformed cells induces F-actin to promote adherens junction-mediated cell-cell adhesion. Mol Carcinog. 2015;54:1181-93 pubmed publisher
  • immunohistochemistry; pig; 1:100
Rodriguez A, Allegrucci C, Alberio R. Modulation of pluripotency in the porcine embryo and iPS cells. PLoS ONE. 2012;7:e49079 pubmed publisher
Walker J, Bleaken B, Romisher A, Alnwibit A, Menko A. In wound repair vimentin mediates the transition of mesenchymal leader cells to a myofibroblast phenotype. Mol Biol Cell. 2018;29:1555-1570 pubmed publisher
Gerhart J, Greenbaum M, Scheinfeld V, Fitzgerald P, Crawford M, Bravo Nuevo A, et al. Myo/Nog cells: targets for preventing the accumulation of skeletal muscle-like cells in the human lens. PLoS ONE. 2014;9:e95262 pubmed publisher
Pleumeekers M, Nimeskern L, Koevoet W, Kops N, Poublon R, Stok K, et al. The in vitro and in vivo capacity of culture-expanded human cells from several sources encapsulated in alginate to form cartilage. Eur Cell Mater. 2014;27:264-80; discussion 278-80 pubmed
Laurent M, Maryvonne L, Le Dreau G, Gwenvaël L, Guillonneau X, Xavier G, et al. Temporal and spatial expression of CCN3 during retina development. Dev Neurobiol. 2012;72:1363-75 pubmed publisher
Pandit S, Sullivan J, Egger V, Borecki A, Oleskevich S. Functional effects of adult human olfactory stem cells on early-onset sensorineural hearing loss. Stem Cells. 2011;29:670-7 pubmed publisher
Hellingman C, Verwiel E, Slagt I, Koevoet W, Poublon R, Nolst Trenité G, et al. Differences in cartilage-forming capacity of expanded human chondrocytes from ear and nose and their gene expression profiles. Cell Transplant. 2011;20:925-40 pubmed publisher
Xu G, Paige J, Jaffrey S. Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling. Nat Biotechnol. 2010;28:868-73 pubmed publisher
Bódi I, Nagy N, Sinka L, Igyártó B, Oláh I. Novel monoclonal antibodies recognise guinea fowl thrombocytes. Acta Vet Hung. 2009;57:239-46 pubmed publisher
Nagy N, Oláh I. Locally applied testosterone is a novel method to influence the development of the avian bursa of Fabricius. J Immunol Methods. 2009;343:97-102 pubmed publisher
Kawaguchi M, Hager H, Wada A, Koyama T, Chang M, Bader D. Identification of a novel intracellular interaction domain essential for Bves function. PLoS ONE. 2008;3:e2261 pubmed publisher
Nagy N, Oláh I. Pyloric tonsil as a novel gut-associated lymphoepithelial organ of the chicken. J Anat. 2007;211:407-11 pubmed
Rouger K, Fornasari B, Armengol V, Jouvion G, Leroux I, Dubreil L, et al. Progenitor cell isolation from muscle-derived cells based on adhesion properties. J Histochem Cytochem. 2007;55:607-18 pubmed
Bimber B, Dettman R, Simon H. Differential regulation of Tbx5 protein expression and sub-cellular localization during heart development. Dev Biol. 2007;302:230-42 pubmed
Bahia D, Avelar L, Vigorosi F, Cioli D, Oliveira G, Mortara R. The distribution of motor proteins in the muscles and flame cells of the Schistosoma mansoni miracidium and primary sporocyst. Parasitology. 2006;133:321-9 pubmed
Weber G, Menko A. Actin filament organization regulates the induction of lens cell differentiation and survival. Dev Biol. 2006;295:714-29 pubmed
Moralez I, Phelps A, Riley B, Raines M, Wirrig E, Snarr B, et al. Muscularizing tissues in the endocardial cushions of the avian heart are characterized by the expression of h1-calponin. Dev Dyn. 2006;235:1648-58 pubmed
Liu H, Meagher C, Moore C, Phillips T. M cells in the follicle-associated epithelium of the rabbit conjunctiva preferentially bind and translocate latex beads. Invest Ophthalmol Vis Sci. 2005;46:4217-23 pubmed
Felföldi B, Imre G, Igyarto B, Iván J, Mihalik R, Lackó E, et al. In ovo vitelline duct ligation results in transient changes of bursal microenvironments. Immunology. 2005;116:267-75 pubmed
Nagy N, Igyarto B, Magyar A, Gazdag E, Palya V, Olah I. Oesophageal tonsil of the chicken. Acta Vet Hung. 2005;53:173-88 pubmed
Kues W, Petersen B, Mysegades W, Carnwath J, Niemann H. Isolation of murine and porcine fetal stem cells from somatic tissue. Biol Reprod. 2005;72:1020-8 pubmed
Nagy N, Bíró E, Takacs A, Pólos M, Magyar A, Oláh I. Peripheral blood fibrocytes contribute to the formation of the avian spleen. Dev Dyn. 2005;232:55-66 pubmed
Trickey W, Vail T, Guilak F. The role of the cytoskeleton in the viscoelastic properties of human articular chondrocytes. J Orthop Res. 2004;22:131-9 pubmed
Pérez Pomares J, Phelps A, Sedmerova M, Wessels A. Epicardial-like cells on the distal arterial end of the cardiac outflow tract do not derive from the proepicardium but are derivatives of the cephalic pericardium. Dev Dyn. 2003;227:56-68 pubmed
Tischer T, Milz S, Maier M, Schieker M, Benjamin M. An immunohistochemical study of the rabbit suprapatella, a sesamoid fibrocartilage in the quadriceps tendon containing aggrecan. J Histochem Cytochem. 2002;50:955-60 pubmed
Li C, Yan R, Wang S. Atrophy of Müller glia and photoreceptor cells in chick retina misexpressing cNSCL2. Invest Ophthalmol Vis Sci. 2001;42:3103-9 pubmed
Pérez Pomares J, Macías López D, García Garrido L, Muñoz Chápuli R. Immunohistochemical evidence for a mesothelial contribution to the ventral wall of the avian aorta. Histochem J. 1999;31:771-9 pubmed
Bellin R, Sernett S, Becker B, Ip W, Huiatt T, Robson R. Molecular characteristics and interactions of the intermediate filament protein synemin. Interactions with alpha-actinin may anchor synemin-containing heterofilaments. J Biol Chem. 1999;274:29493-9 pubmed
Muñoz Chápuli R, Pérez Pomares J, Macias D, García Garrido L, Carmona R, Gonzalez M. Differentiation of hemangioblasts from embryonic mesothelial cells? A model on the origin of the vertebrate cardiovascular system. Differentiation. 1999;64:133-41 pubmed
Tanaka Kamioka K, Kamioka H, Ris H, Lim S. Osteocyte shape is dependent on actin filaments and osteocyte processes are unique actin-rich projections. J Bone Miner Res. 1998;13:1555-68 pubmed
Hemken P, Bellin R, Sernett S, Becker B, Huiatt T, Robson R. Molecular characteristics of the novel intermediate filament protein paranemin. Sequence reveals EAP-300 and IFAPa-400 are highly homologous to paranemin. J Biol Chem. 1997;272:32489-99 pubmed
Pérez Pomares J, Macias D, García Garrido L, Muñoz Chápuli R. Contribution of the primitive epicardium to the subepicardial mesenchyme in hamster and chick embryos. Dev Dyn. 1997;210:96-105 pubmed
Isaacs W, Cook R, Van Atta J, Redmond C, Fulton A. Assembly of vimentin in cultured cells varies with cell type. J Biol Chem. 1989;264:17953-60 pubmed
Isaacs W, Kim I, Struve A, Fulton A. Association of titin and myosin heavy chain in developing skeletal muscle. Proc Natl Acad Sci U S A. 1992;89:7496-500 pubmed
product information
Internal ID :
3202
Name :
AMF-17b
Depositor Name :
Fulton, A.B.
Depositor Institution :
University of Iowa
Date Deposited :
11/21/95
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Vimentin
Antigen Species :
Chicken
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Bovine,Canine,Chicken,Human,Porcine,Rabbit
Species Tested (legacy) :
chicken, human, bovine, dog (all mammals tested except rodents)
Initial Publication Pubmed ID :
2808358
Depositor Notes (Special Instructions) :
AMF-17b recognizes vimentin in all mammal species tested except rodents.
Collections :
Cytoskeleton,Human,Stem cell
Search Keywords :
Fulton, Alice B., Vimentin, Chicken, MIgG1, kappa light chain, Human/Chicken/Bovine/Canine/Porcine/Rabbit, VIM, , AB_528505, monoclonal
Antigen Molecular Weight :
Predicted: 53kDa; Apparent: 53kDa
Gene :
VIM
Uniprot ID :
P09654
Antibody Registry ID :
AB_528505
Immunogen :
Embryonic myofibril
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Epitope Mapped :
Yes
Epitope Location or Sequence :
Located close (upstream) to amino acid position 220
Epitope Map PubMed ID :
2808358
Recommended Applications :
FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
11726609 10661320 21054934 19265698 17593219 16162275 12070274 10234810 22038708 15959976 16502418 24706178
Immunofluorescence Pubmed IDs :
9405460 1502160 9337131 23145076 9783544 10506213 14656671 10661320 15616223 16249501 15580570 24736495 21312317 12701099
Western Blot Pubmed IDs :
2808358 10506213 24976598 16678812
Immunoprecipitation Pubmed IDs :
2808358 1502160
FFPE Pubmed IDs :
9337131 10661320 16249501 10234810 12701099 15959976 16502418 24706178
Pubmed IDs :
16740180 17045582 17312016
Depositor Growth Medium :
Iscove's
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Biol. Chem. 264, 17953-17960.; Dev. Dyn. 232, 55-66.; Acta Vet. Hung. 53(2), 173-188.; PNAS 106(46), 19310-19315.; J. Immunol. Methods 343, 97-102.; Stem Cells 29, 670-677.; Acta Vet. Hung. 57(2), 239-246.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US