product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
lamin Dm0
catalog :
ADL84.12
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
ADL84.12
reactivity :
Drosophila melanogaster
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 33
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; Drosophila melanogaster; 1:500; loading ...; fig 2c
Kanca O, ZIRIN J, García Marqués J, Knight S, Yang Zhou D, Amador G, et al. An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms. elife. 2019;8: pubmed publisher
  • western blot; Drosophila melanogaster; 1:1000; fig s1
Hicks L, Liu G, Ukken F, Lu S, Bollinger K, O Connor Giles K, et al. Depletion or over-expression of Sh3px1 results in dramatic changes in cell morphology. Biol Open. 2015;4:1448-61 pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:50; fig 10
Zhan L, Hanson K, Kim S, Tare A, Tibbetts R. Identification of genetic modifiers of TDP-43 neurotoxicity in Drosophila. PLoS ONE. 2013;8:e57214 pubmed publisher
Swider Z, Ng R, Varadarajan R, Fagerstrom C, Rusan N. Fascetto interacting protein ensures proper cytokinesis and ploidy. Mol Biol Cell. 2019;30:992-1007 pubmed publisher
Mehsen H, Boudreau V, Garrido D, Bourouh M, Larouche M, Maddox P, et al. PP2A-B55 promotes nuclear envelope reformation after mitosis in Drosophila. J Cell Biol. 2018;217:4106-4123 pubmed publisher
Kistler K, Trcek T, Hurd T, Chen R, Liang F, Sall J, et al. Phase transitioned nuclear Oskar promotes cell division of Drosophila primordial germ cells. elife. 2018;7: pubmed publisher
Lu K, Yamashita Y. Germ cell connectivity enhances cell death in response to DNA damage in the Drosophila testis. elife. 2017;6: pubmed publisher
Ding Z, Wang Y, Huang Y, Lee M, Tseng M, Chi Y, et al. Outer nuclear membrane protein Kuduk modulates the LINC complex and nuclear envelope architecture. J Cell Biol. 2017;216:2827-2841 pubmed publisher
Tsou W, Qiblawi S, Hosking R, Gomez C, Todi S. Polyglutamine length-dependent toxicity from ?1ACT in Drosophila models of spinocerebellar ataxia type 6. Biol Open. 2016;5:1770-1775 pubmed publisher
Hayashi D, Tanabe K, Katsube H, Inoue Y. B-type nuclear lamin and the nuclear pore complex Nup107-160 influences maintenance of the spindle envelope required for cytokinesis in Drosophila male meiosis. Biol Open. 2016;5:1011-21 pubmed publisher
George C, Bozler J, Nguyen H, Bosco G. Condensins are Required for Maintenance of Nuclear Architecture. Cells. 2014;3:865-82 pubmed publisher
Zhang L, Beaucher M, Cheng Y, Rong Y. Coordination of transposon expression with DNA replication in the targeting of telomeric retrotransposons in Drosophila. EMBO J. 2014;33:1148-58 pubmed publisher
Barton L, Wilmington S, Martin M, Skopec H, Lovander K, Pinto B, et al. Unique and shared functions of nuclear lamina LEM domain proteins in Drosophila. Genetics. 2014;197:653-65 pubmed publisher
Sanghavi P, Laxani S, Li X, Bullock S, Gonsalvez G. Dynein associates with oskar mRNPs and is required for their efficient net plus-end localization in Drosophila oocytes. PLoS ONE. 2013;8:e80605 pubmed publisher
Ali Murthy Z, Lott S, Eisen M, Kornberg T. An essential role for zygotic expression in the pre-cellular Drosophila embryo. PLoS Genet. 2013;9:e1003428 pubmed publisher
Buster D, Daniel S, Nguyen H, Windler S, Skwarek L, Peterson M, et al. SCFSlimb ubiquitin ligase suppresses condensin II-mediated nuclear reorganization by degrading Cap-H2. J Cell Biol. 2013;201:49-63 pubmed publisher
Milon B, Cheng H, Tselebrovsky M, Lavrov S, Nenasheva V, Mikhaleva E, et al. Role of histone deacetylases in gene regulation at nuclear lamina. PLoS ONE. 2012;7:e49692 pubmed publisher
Villa Cuesta E, Sage B, Tatar M. A role for Drosophila dFoxO and dFoxO 5'UTR internal ribosomal entry sites during fasting. PLoS ONE. 2010;5:e11521 pubmed publisher
Civelekoglu Scholey G, Tao L, Brust Mascher I, Wollman R, Scholey J. Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope. J Cell Biol. 2010;188:49-68 pubmed publisher
Wang L, Lam G, Thummel C. Med24 and Mdh2 are required for Drosophila larval salivary gland cell death. Dev Dyn. 2010;239:954-64 pubmed publisher
Schulze S, Curio Penny B, Speese S, Dialynas G, Cryderman D, McDonough C, et al. A comparative study of Drosophila and human A-type lamins. PLoS ONE. 2009;4:e7564 pubmed publisher
Landmann F, Orsi G, Loppin B, Sullivan W. Wolbachia-mediated cytoplasmic incompatibility is associated with impaired histone deposition in the male pronucleus. PLoS Pathog. 2009;5:e1000343 pubmed publisher
Furukawa K, Ishida K, Tsunoyama T, Toda S, Osoda S, Horigome T, et al. A-type and B-type lamins initiate layer assembly at distinct areas of the nuclear envelope in living cells. Exp Cell Res. 2009;315:1181-9 pubmed publisher
Bass B, Cullen K, McCall K. The axon guidance gene lola is required for programmed cell death in the Drosophila ovary. Dev Biol. 2007;304:771-85 pubmed
Osouda S, Nakamura Y, de Saint Phalle B, McConnell M, Horigome T, Sugiyama S, et al. Null mutants of Drosophila B-type lamin Dm(0) show aberrant tissue differentiation rather than obvious nuclear shape distortion or specific defects during cell proliferation. Dev Biol. 2005;284:219-32 pubmed
Schulze S, Curio Penny B, Li Y, Imani R, Rydberg L, Geyer P, et al. Molecular genetic analysis of the nested Drosophila melanogaster lamin C gene. Genetics. 2005;171:185-96 pubmed
Martin D, Baehrecke E. Caspases function in autophagic programmed cell death in Drosophila. Development. 2004;131:275-84 pubmed
Rzepecki R, Fisher P. In vivo phosphorylation of Drosophila melanogaster nuclear lamins during both interphase and mitosis. Cell Mol Biol Lett. 2002;7:859-76 pubmed
Guillemin K, Williams T, Krasnow M. A nuclear lamin is required for cytoplasmic organization and egg polarity in Drosophila. Nat Cell Biol. 2001;3:848-51 pubmed
Sasse B, Aebi U, Stuurman N. A tailless Drosophila lamin Dm0 fragment reveals lateral associations of dimers. J Struct Biol. 1998;123:56-66 pubmed
Stuurman N, Sasse B, Fisher P. Intermediate filament protein polymerization: molecular analysis of Drosophila nuclear lamin head-to-tail binding. J Struct Biol. 1996;117:1-15 pubmed
Stuurman N, Maus N, Fisher P. Interphase phosphorylation of the Drosophila nuclear lamin: site-mapping using a monoclonal antibody. J Cell Sci. 1995;108 ( Pt 9):3137-44 pubmed
Maus N, Stuurman N, Fisher P. Disassembly of the Drosophila nuclear lamina in a homologous cell-free system. J Cell Sci. 1995;108 ( Pt 5):2027-35 pubmed
product information
Internal ID :
3154
Name :
ADL84.12
Depositor Name :
Fisher, P. A.
Depositor Institution :
Pharmacological Sciences, SUNY at Stony Brook
Depositor Website :
http://www.pharm.stonybrook.edu/faculty/f/fisher
Date Deposited :
3/11/03
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
lamin Dm0
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila, not Sf9 lamin
Initial Publication Pubmed ID :
8537453
Depositor Notes (Special Instructions) :
Does not react with Sf9 lamin. Reacts only with Dm1 and Dmmit, not Dm2 or Dmmit-s; phosphorylation of S(25) abolishes reactivity; does not react with lamin C.
Collections :
Cytoskeleton,Drosophila antigens,Nucleus
Search Keywords :
Fisher, P. A., lamin Dm0, Drosophila, MIgG1, Drosophila, Lam, Dm0; lamin Dm0; Dm1; Dm2, AB_528338, monoclonal, Cytoskeletal elements/Drosophila antigens/Nucleus, Immunofluorescence/Western Blot/Immunoprecipitation
Antigen Molecular Weight :
Predicted: 71 kDa; Apparent: 74 kDa
Gene :
Lam
Alternate Gene Name(s) :
Dm0; lamin Dm0; Dm1; Dm2
Uniprot ID :
P08928
Antibody Registry ID :
AB_528338
Synonyms (Alternate Clone Names) :
ADL84; alphaL22-28
Immunogen :
Recombinant total protein
Immunogen Sequence :
Total protein
Clonality :
Monoclonal
Myeloma Strain :
NS0
Epitope Mapped :
Yes
Epitope Location or Sequence :
aa 22-28; RPPSAGP
Epitope Map PubMed ID :
8537453
Recommended Applications :
Immunofluorescence,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Yes
Immunofluorescence Pubmed IDs :
8537453 19210986 15965247 19855837 7657722 11533666 9774545 19210986 19300496 23226217 14668412 17336958 20063412 28809158
Western Blot Pubmed IDs :
8537453 12378269 24700158 14668412
Immunoprecipitation Pubmed IDs :
8776884 8537453
Pubmed IDs :
15996653
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Cell Sci. 108, 3137-3144.; J. Struct. Biol. 117(1), 1-15.; Exp. Cell Res. 315(7), 1181-1189.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US