product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
lamin Dm0
catalog :
ADL67.10
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
ADL67.10
reactivity :
Drosophila melanogaster
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
citations: 43
Published Application/Species/Sample/Dilution | Reference |
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Pickersgill H, Kalverda B, de Wit E, Talhout W, Fornerod M, van Steensel B. Characterization of the Drosophila melanogaster genome at the nuclear lamina. Nat Genet. 2006;38:1005-14 pubmed
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Mills K, Daish T, Harvey K, Pfleger C, Hariharan I, Kumar S. The Drosophila melanogaster Apaf-1 homologue ARK is required for most, but not all, programmed cell death. J Cell Biol. 2006;172:809-15 pubmed
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Osouda S, Nakamura Y, de Saint Phalle B, McConnell M, Horigome T, Sugiyama S, et al. Null mutants of Drosophila B-type lamin Dm(0) show aberrant tissue differentiation rather than obvious nuclear shape distortion or specific defects during cell proliferation. Dev Biol. 2005;284:219-32 pubmed
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Schulze S, Curio Penny B, Li Y, Imani R, Rydberg L, Geyer P, et al. Molecular genetic analysis of the nested Drosophila melanogaster lamin C gene. Genetics. 2005;171:185-96 pubmed
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Pang M, McConnell M, Fisher P. The Drosophila mus 308 gene product, implicated in tolerance of DNA interstrand crosslinks, is a nuclear protein found in both ovaries and embryos. DNA Repair (Amst). 2005;4:971-82 pubmed
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Lenz Böhme B, Wismar J, Fuchs S, Reifegerste R, Buchner E, Betz H, et al. Insertional mutation of the Drosophila nuclear lamin Dm0 gene results in defective nuclear envelopes, clustering of nuclear pore complexes, and accumulation of annulate lamellae. J Cell Biol. 1997;137:1001-16 pubmed
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Stuurman N, Sasse B, Fisher P. Intermediate filament protein polymerization: molecular analysis of Drosophila nuclear lamin head-to-tail binding. J Struct Biol. 1996;117:1-15 pubmed
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Stuurman N, Maus N, Fisher P. Interphase phosphorylation of the Drosophila nuclear lamin: site-mapping using a monoclonal antibody. J Cell Sci. 1995;108 ( Pt 9):3137-44 pubmed
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Riemer D, Stuurman N, Berrios M, Hunter C, Fisher P, Weber K. Expression of Drosophila lamin C is developmentally regulated: analogies with vertebrate A-type lamins. J Cell Sci. 1995;108 ( Pt 10):3189-98 pubmed
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product information
Internal ID :
3142
Name :
ADL67.10
Depositor Name :
Fisher, P. A.
Depositor Institution :
Pharmacological Sciences, SUNY at Stony Brook
Depositor Website :
http://www.pharm.stonybrook.edu/faculty/f/fisher
Date Deposited :
3/11/03
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
lamin Dm0
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Drosophila,Mosquito,Spodoptera frugiperda
Species Tested (legacy) :
Drosophila, Sf9 lamin
Initial Publication Pubmed ID :
7593280
Depositor Notes (Special Instructions) :
Reacts with Sf9 lamin. Reacts with all Dm isoforms; does not react with lamin C. Epitope between aa 548-620 (tail region).
Collections :
Cytoskeleton,Drosophila antigens,Nucleus
Search Keywords :
Fisher, P. A., lamin Dm0, Drosophila, MIgG1, Drosophila/Spodoptera frugiperda/Mosquito, Lam, Dm0; lamin Dm0; Dm1; Dm2, AB_528336, monoclonal, Cytoskeletal elements/Drosophila antigens/Nucleus, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation
Antigen Molecular Weight :
Predicted: 71 kDa; Apparent: 74 and 76 kDa
Gene :
Lam
Alternate Gene Name(s) :
Dm0; lamin Dm0; Dm1; Dm2
Uniprot ID :
P08928
Antibody Registry ID :
AB_528336
Synonyms (Alternate Clone Names) :
ADL67; alphaL528-622
Immunogen :
Recombinant total protein
Immunogen Sequence :
Total protein
Clonality :
Monoclonal
Myeloma Strain :
NS0
Epitope Mapped :
Yes
Epitope Location or Sequence :
aa 548-620 [Depositor Notes] or aa 528-622 [PMID 8776884].
Epitope Map PubMed ID :
8776884
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
7593280 29174892
Immunofluorescence Pubmed IDs :
7593280 9166402 15996653 16878134 27164127 22393432 15965247 26963718 25281900 20684766 22649080
Western Blot Pubmed IDs :
7593280 8537453 22467865 9166402 15996653 16878134 27164127 15961355
Immunoprecipitation Pubmed IDs :
8776884 8537453
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Cell Sci. 108, 3189-3198.; J. Struct. Biol. 117(1), 1-15.; J. Cell Sci. 110, 2519-2532.; J. Cell Sci. 10138: doi:10.1242/jcs.101386.
more info or order :
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