product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
lamin Dm0
catalog :
ADL67.10
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
ADL67.10
reactivity :
Drosophila melanogaster
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 43
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; Drosophila melanogaster; 1:50; loading ...; fig s1b
Chen D, Dale R, Lei E. Shep regulates Drosophila neuronal remodeling by controlling transcription of its chromatin targets. Development. 2018;145: pubmed publisher
  • western blot; Drosophila melanogaster; 1:1000; loading ...; fig 4f
Stepien B, Oppitz C, Gerlach D, Dag U, Novatchkova M, Krüttner S, et al. RNA-binding profiles of Drosophila CPEB proteins Orb and Orb2. Proc Natl Acad Sci U S A. 2016;113:E7030-E7038 pubmed publisher
  • immunohistochemistry - paraffin section; Drosophila melanogaster; 1:10; fig 2b
Beck K, Ehmann N, Andlauer T, Ljaschenko D, Strecker K, Fischer M, et al. Loss of the Coffin-Lowry syndrome-associated gene RSK2 alters ERK activity, synaptic function and axonal transport in Drosophila motoneurons. Dis Model Mech. 2015;8:1389-400 pubmed publisher
  • immunohistochemistry; Drosophila melanogaster; 1:100; loading ...; fig 5a
Pflanz R, Voigt A, Yakulov T, Jäckle H. Drosophila gene tao-1 encodes proteins with and without a Ste20 kinase domain that affect cytoskeletal architecture and cell migration differently. Open Biol. 2015;5:140161 pubmed publisher
Viets K, Sauria M, Chernoff C, Rodriguez Viales R, Echterling M, Anderson C, et al. Characterization of Button Loci that Promote Homologous Chromosome Pairing and Cell-Type-Specific Interchromosomal Gene Regulation. Dev Cell. 2019;51:341-356.e7 pubmed publisher
Kneuss E, Munafò M, Eastwood E, Deumer U, Preall J, Hannon G, et al. Specialization of the Drosophila nuclear export family protein Nxf3 for piRNA precursor export. Genes Dev. 2019;33:1208-1220 pubmed publisher
Fabry M, Ciabrelli F, Munafò M, Eastwood E, Kneuss E, Falciatori I, et al. piRNA-guided co-transcriptional silencing coopts nuclear export factors. elife. 2019;8: pubmed publisher
Andreyeva E, Ogienko A, Dubatolova T, Oshchepkova A, Kozhevnikova E, Ivankin A, et al. A toolset to study functions of Cytosolic non-specific dipeptidase 2 (CNDP2) using Drosophila as a model organism. BMC Genet. 2019;20:31 pubmed publisher
Kotov A, Adashev V, Godneeva B, Ninova M, Shatskikh A, Bazylev S, et al. piRNA silencing contributes to interspecies hybrid sterility and reproductive isolation in Drosophila melanogaster. Nucleic Acids Res. 2019;47:4255-4271 pubmed publisher
Cornelison G, Levy S, Jenson T, Frost B. Tau-induced nuclear envelope invagination causes a toxic accumulation of mRNA in Drosophila. Aging Cell. 2019;18:e12847 pubmed publisher
Mehsen H, Boudreau V, Garrido D, Bourouh M, Larouche M, Maddox P, et al. PP2A-B55 promotes nuclear envelope reformation after mitosis in Drosophila. J Cell Biol. 2018;217:4106-4123 pubmed publisher
Yamamoto Hino M, Katsumata E, Suzuki E, Maeda Y, Kinoshita T, Goto S. Nuclear envelope localization of PIG-B is essential for GPI-anchor synthesis in Drosophila. J Cell Sci. 2018;131: pubmed publisher
Mallik M, Catinozzi M, Hug C, Zhang L, Wagner M, Bussmann J, et al. Xrp1 genetically interacts with the ALS-associated FUS orthologue caz and mediates its toxicity. J Cell Biol. 2018;217:3947-3964 pubmed publisher
Courgeon M, He D, Liu H, Legent K, Treisman J. The Drosophila Epidermal Growth Factor Receptor does not act in the nucleus. J Cell Sci. 2018;131: pubmed publisher
Deutschman E, Ward J, Ho A Lim K, Alban T, Zhang D, Willard B, et al. Comparing and Contrasting the Effects of Drosophila Condensin II Subunit dCAP-D3 Overexpression and Depletion in Vivo. Genetics. 2018;210:531-546 pubmed publisher
Strunov A, Boldyreva L, Andreyeva E, Pavlova G, Popova J, Razuvaeva A, et al. Ultrastructural analysis of mitotic Drosophila S2 cells identifies distinctive microtubule and intracellular membrane behaviors. BMC Biol. 2018;16:68 pubmed publisher
Johnson P, Doe C, Lai S. Drosophila nucleostemin 3 is required to maintain larval neuroblast proliferation. Dev Biol. 2018;440:1-12 pubmed publisher
Názer E, Dale R, Chinen M, Radmanesh B, Lei E. Argonaute2 and LaminB modulate gene expression by controlling chromatin topology. PLoS Genet. 2018;14:e1007276 pubmed publisher
Baron O, Boudi A, Dias C, Schilling M, Nölle A, Vizcay Barrena G, et al. Stall in Canonical Autophagy-Lysosome Pathways Prompts Nucleophagy-Based Nuclear Breakdown in Neurodegeneration. Curr Biol. 2017;27:3626-3642.e6 pubmed publisher
Cheng M, Andrejka L, Vorster P, Hinman A, Lipsick J. The Drosophila LIN54 homolog Mip120 controls two aspects of oogenesis. Biol Open. 2017;6:967-978 pubmed publisher
Buxa M, Slotman J, van Royen M, Paul M, Houtsmuller A, Renkawitz R. Insulator speckles associated with long-distance chromatin contacts. Biol Open. 2016;5:1266-74 pubmed publisher
Wei W, Wang H, Li X, Fang N, Yang S, Liu H, et al. Cloning and Characterization of Sf9 Cell Lamin and the Lamin Conformational Changes during Autographa californica multiple nucleopolyhedrovirus Infection. Viruses. 2016;8: pubmed publisher
Fabbretti F, Iannetti I, Guglielmi L, Perconti S, Evangelistella C, Proietti de Santis L, et al. Confocal Analysis of Nuclear Lamina Behavior during Male Meiosis and Spermatogenesis in Drosophila melanogaster. PLoS ONE. 2016;11:e0151231 pubmed publisher
Ramdas N, Shivashankar G. Cytoskeletal control of nuclear morphology and chromatin organization. J Mol Biol. 2015;427:695-706 pubmed publisher
Bosch J, Sumabat T, Hafezi Y, Pellock B, Gandhi K, Hariharan I. The Drosophila F-box protein Fbxl7 binds to the protocadherin fat and regulates Dachs localization and Hippo signaling. elife. 2014;3:e03383 pubmed publisher
Zhang L, Beaucher M, Cheng Y, Rong Y. Coordination of transposon expression with DNA replication in the targeting of telomeric retrotransposons in Drosophila. EMBO J. 2014;33:1148-58 pubmed publisher
Lim S, Boyle P, Chinen M, Dale R, Lei E. Genome-wide localization of exosome components to active promoters and chromatin insulators in Drosophila. Nucleic Acids Res. 2013;41:2963-80 pubmed publisher
Sienski G, Donertas D, Brennecke J. Transcriptional silencing of transposons by Piwi and maelstrom and its impact on chromatin state and gene expression. Cell. 2012;151:964-80 pubmed publisher
Cohen J, Brinton E, Ito M, Jacobson T. Understanding Statin Use in America and Gaps in Patient Education (USAGE): an internet-based survey of 10,138 current and former statin users. J Clin Lipidol. 2012;6:208-15 pubmed publisher
Kim M, Bucciarelli E, Morton D, Williams B, Blake Hodek K, Pellacani C, et al. Bypassing the Greatwall-Endosulfine pathway: plasticity of a pivotal cell-cycle regulatory module in Drosophila melanogaster and Caenorhabditis elegans. Genetics. 2012;191:1181-97 pubmed publisher
Elias M, Pronovost S, Cahill K, Beckerle M, Kadrmas J. A crucial role for Ras suppressor-1 (RSU-1) revealed when PINCH and ILK binding is disrupted. J Cell Sci. 2012;125:3185-94 pubmed publisher
Zaremba Czogalla M, Piekarowicz K, Wachowicz K, Kozioł K, Dubińska Magiera M, Rzepecki R. The different function of single phosphorylation sites of Drosophila melanogaster lamin Dm and lamin C. PLoS ONE. 2012;7:e32649 pubmed publisher
Sharakhova M, George P, Brusentsova I, Leman S, Bailey J, Smith C, et al. Genome mapping and characterization of the Anopheles gambiae heterochromatin. BMC Genomics. 2010;11:459 pubmed publisher
Civelekoglu Scholey G, Tao L, Brust Mascher I, Wollman R, Scholey J. Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope. J Cell Biol. 2010;188:49-68 pubmed publisher
Pickersgill H, Kalverda B, de Wit E, Talhout W, Fornerod M, van Steensel B. Characterization of the Drosophila melanogaster genome at the nuclear lamina. Nat Genet. 2006;38:1005-14 pubmed
Mills K, Daish T, Harvey K, Pfleger C, Hariharan I, Kumar S. The Drosophila melanogaster Apaf-1 homologue ARK is required for most, but not all, programmed cell death. J Cell Biol. 2006;172:809-15 pubmed
Osouda S, Nakamura Y, de Saint Phalle B, McConnell M, Horigome T, Sugiyama S, et al. Null mutants of Drosophila B-type lamin Dm(0) show aberrant tissue differentiation rather than obvious nuclear shape distortion or specific defects during cell proliferation. Dev Biol. 2005;284:219-32 pubmed
Schulze S, Curio Penny B, Li Y, Imani R, Rydberg L, Geyer P, et al. Molecular genetic analysis of the nested Drosophila melanogaster lamin C gene. Genetics. 2005;171:185-96 pubmed
Pang M, McConnell M, Fisher P. The Drosophila mus 308 gene product, implicated in tolerance of DNA interstrand crosslinks, is a nuclear protein found in both ovaries and embryos. DNA Repair (Amst). 2005;4:971-82 pubmed
Lenz Böhme B, Wismar J, Fuchs S, Reifegerste R, Buchner E, Betz H, et al. Insertional mutation of the Drosophila nuclear lamin Dm0 gene results in defective nuclear envelopes, clustering of nuclear pore complexes, and accumulation of annulate lamellae. J Cell Biol. 1997;137:1001-16 pubmed
Stuurman N, Sasse B, Fisher P. Intermediate filament protein polymerization: molecular analysis of Drosophila nuclear lamin head-to-tail binding. J Struct Biol. 1996;117:1-15 pubmed
Stuurman N, Maus N, Fisher P. Interphase phosphorylation of the Drosophila nuclear lamin: site-mapping using a monoclonal antibody. J Cell Sci. 1995;108 ( Pt 9):3137-44 pubmed
Riemer D, Stuurman N, Berrios M, Hunter C, Fisher P, Weber K. Expression of Drosophila lamin C is developmentally regulated: analogies with vertebrate A-type lamins. J Cell Sci. 1995;108 ( Pt 10):3189-98 pubmed
product information
Internal ID :
3142
Name :
ADL67.10
Depositor Name :
Fisher, P. A.
Depositor Institution :
Pharmacological Sciences, SUNY at Stony Brook
Depositor Website :
http://www.pharm.stonybrook.edu/faculty/f/fisher
Date Deposited :
3/11/03
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
lamin Dm0
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Drosophila,Mosquito,Spodoptera frugiperda
Species Tested (legacy) :
Drosophila, Sf9 lamin
Initial Publication Pubmed ID :
7593280
Depositor Notes (Special Instructions) :
Reacts with Sf9 lamin. Reacts with all Dm isoforms; does not react with lamin C. Epitope between aa 548-620 (tail region).
Collections :
Cytoskeleton,Drosophila antigens,Nucleus
Search Keywords :
Fisher, P. A., lamin Dm0, Drosophila, MIgG1, Drosophila/Spodoptera frugiperda/Mosquito, Lam, Dm0; lamin Dm0; Dm1; Dm2, AB_528336, monoclonal, Cytoskeletal elements/Drosophila antigens/Nucleus, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation
Antigen Molecular Weight :
Predicted: 71 kDa; Apparent: 74 and 76 kDa
Gene :
Lam
Alternate Gene Name(s) :
Dm0; lamin Dm0; Dm1; Dm2
Uniprot ID :
P08928
Antibody Registry ID :
AB_528336
Synonyms (Alternate Clone Names) :
ADL67; alphaL528-622
Immunogen :
Recombinant total protein
Immunogen Sequence :
Total protein
Clonality :
Monoclonal
Myeloma Strain :
NS0
Epitope Mapped :
Yes
Epitope Location or Sequence :
aa 548-620 [Depositor Notes] or aa 528-622 [PMID 8776884].
Epitope Map PubMed ID :
8776884
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
7593280 29174892
Immunofluorescence Pubmed IDs :
7593280 9166402 15996653 16878134 27164127 22393432 15965247 26963718 25281900 20684766 22649080
Western Blot Pubmed IDs :
7593280 8537453 22467865 9166402 15996653 16878134 27164127 15961355
Immunoprecipitation Pubmed IDs :
8776884 8537453
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Cell Sci. 108, 3189-3198.; J. Struct. Biol. 117(1), 1-15.; J. Cell Sci. 110, 2519-2532.; J. Cell Sci. 10138: doi:10.1242/jcs.101386.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US