product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
lamin Dm0
catalog :
ADL195
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
ADL195
reactivity :
fruit fly
application :
western blot, immunocytochemistry
citations: 9
Published Application/Species/Sample/DilutionReference
  • western blot; fruit fly; 1:200; fig 4f
Groen C, Jayo A, Parsons M, Tootle T. Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture. Mol Biol Cell. 2015;26:1901-17 pubmed publisher
Beck K, Ehmann N, Andlauer T, Ljaschenko D, Strecker K, Fischer M, et al. Loss of the Coffin-Lowry syndrome-associated gene RSK2 alters ERK activity, synaptic function and axonal transport in Drosophila motoneurons. Dis Model Mech. 2015;8:1389-400 pubmed publisher
Kohwi M, Lupton J, Lai S, Miller M, Doe C. Developmentally regulated subnuclear genome reorganization restricts neural progenitor competence in Drosophila. Cell. 2013;152:97-108 pubmed publisher
Milon B, Cheng H, Tselebrovsky M, Lavrov S, Nenasheva V, Mikhaleva E, et al. Role of histone deacetylases in gene regulation at nuclear lamina. PLoS ONE. 2012;7:e49692 pubmed publisher
Gilliland W, Hughes S, Cotitta J, Takeo S, Xiang Y, Hawley R. The multiple roles of mps1 in Drosophila female meiosis. PLoS Genet. 2007;3:e113 pubmed
Wagner N, Kagermeier B, Loserth S, Krohne G. The Drosophila melanogaster LEM-domain protein MAN1. Eur J Cell Biol. 2006;85:91-105 pubmed
Qi H, Rath U, Wang D, Xu Y, Ding Y, Zhang W, et al. Megator, an essential coiled-coil protein that localizes to the putative spindle matrix during mitosis in Drosophila. Mol Biol Cell. 2004;15:4854-65 pubmed
Wagner N, Weber D, Seitz S, Krohne G. The lamin B receptor of Drosophila melanogaster. J Cell Sci. 2004;117:2015-28 pubmed
Klapper M, Exner K, Kempf A, Gehrig C, Stuurman N, Fisher P, et al. Assembly of A- and B-type lamins studied in vivo with the baculovirus system. J Cell Sci. 1997;110 ( Pt 20):2519-32 pubmed
product information
Internal ID :
3124
Name :
ADL195
Depositor Name :
Fisher, P. A.
Depositor Institution :
Pharmacological Sciences, SUNY at Stony Brook
Depositor Website :
http://www.pharm.stonybrook.edu/faculty/f/fisher
Date Deposited :
3/11/03
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
lamin Dm0
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgM
Isotype for catalog (legacy) :
IgM
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila, not Sf9 lamin
Initial Publication Pubmed ID :
9372441
Depositor Notes (Special Instructions) :
Does not react with Sf9 lamin. Reacts with all Dm isoforms; does not react with lamin C. Epitope between aa 548-620 (tail region).
Collections :
Cytoskeleton,Drosophila antigens,Nucleus
Search Keywords :
Fisher, P. A., lamin Dm0, Drosophila, MIgM, Drosophila, Lam, Dm0; lamin Dm0; Dm1; Dm2, AB_528333, monoclonal, Cytoskeletal elements/Drosophila antigens/Nucleus, Immunofluorescence/Western Blot
Antigen Molecular Weight :
Predicted: 71 kDa; Apparent: 74 and 76 kDa
Gene :
Lam
Alternate Gene Name(s) :
Dm0; lamin Dm0; Dm1; Dm2
Uniprot ID :
P08928
Antibody Registry ID :
AB_528333
Synonyms (Alternate Clone Names) :
ADL84; alphaL22-28
Immunogen :
Recombinant total protein
Immunogen Sequence :
Total protein
Clonality :
Monoclonal
Myeloma Strain :
NS0
Epitope Mapped :
Yes
Epitope Location or Sequence :
aa 548-620 [Depositor Notes] or aa 542-622 [PMID 9372441]
Epitope Map PubMed ID :
9372441
Recommended Applications :
Immunofluorescence,Western Blot
Immunoblotting (legacy) :
Yes
Immunofluorescence Pubmed IDs :
9372441 17630834 15356261
Western Blot Pubmed IDs :
9372441 23226217 25808493
Pubmed IDs :
23332748 15054108 16439308 26398944
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Cell Sci. 110, 2519-2532.; Cell 152, 97-108.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US