ecdysone receptor (EcR-B1) | Developmental Studies Hybridoma Bank AD4.4(EcR-B1) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

ecdysone receptor (EcR-B1)

catalog :

AD4.4(EcR-B1)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AD4.4(EcR-B1)

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 21

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; fruit fly; 1:10; tbl 1	Alvarez Rivero J, Moris Sanz M, Estacio Gómez A, Montoliu Nerin M, Diaz Benjumea F, Herrero P. Variability in the number of abdominal leucokinergic neurons in adult Drosophila melanogaster. J Comp Neurol. 2017;525:639-660 pubmed publisher
western blot; fruit fly; fig 6	Xie X, Hsu F, Gao X, Xu W, Ni J, Xing Y, et al. CDK8-Cyclin C Mediates Nutritional Regulation of Developmental Transitions through the Ecdysone Receptor in Drosophila. PLoS Biol. 2015;13:e1002207 pubmed publisher
	Romani P, Gargiulo G, Cavaliere V. The ecdysone receptor signalling regulates microvilli formation in follicular epithelial cells. Cell Mol Life Sci. 2016;73:409-25 pubmed publisher
	McParland A, Follansbee T, Vesenka G, Panaitiu A, Ganter G. Steroid Receptor Isoform Expression in Drosophila Nociceptor Neurons Is Required for Normal Dendritic Arbor and Sensitivity. PLoS ONE. 2015;10:e0140785 pubmed publisher
	Chawla G, Sokol N. Hormonal activation of let-7-C microRNAs via EcR is required for adult Drosophila melanogaster morphology and function. Development. 2012;139:1788-97 pubmed publisher
	Schwedes C, Tulsiani S, Carney G. Ecdysone receptor expression and activity in adult Drosophila melanogaster. J Insect Physiol. 2011;57:899-907 pubmed publisher
	Bernardi F, Romani P, Tzertzinis G, Gargiulo G, Cavaliere V. EcR-B1 and Usp nuclear hormone receptors regulate expression of the VM32E eggshell gene during Drosophila oogenesis. Dev Biol. 2009;328:541-51 pubmed publisher
	Romani P, Bernardi F, Hackney J, Dobens L, Gargiulo G, Cavaliere V. Cell survival and polarity of Drosophila follicle cells require the activity of ecdysone receptor B1 isoform. Genetics. 2009;181:165-75 pubmed publisher
	Foulk M, Liang C, Wu N, Blitzblau H, Smith H, Alam D, et al. Ecdysone induces transcription and amplification in Sciara coprophila DNA puff II/9A. Dev Biol. 2006;299:151-63 pubmed
	Santos J, Pollak E, Rexer K, Molnar L, Wegener C. Morphology and metamorphosis of the peptidergic Va neurons and the median nerve system of the fruit fly, Drosophila melanogaster. Cell Tissue Res. 2006;326:187-99 pubmed
	Cakouros D, Daish T, Mills K, Kumar S. An arginine-histone methyltransferase, CARMER, coordinates ecdysone-mediated apoptosis in Drosophila cells. J Biol Chem. 2004;279:18467-71 pubmed
	Schubiger M, Tomita S, Sung C, Robinow S, Truman J. Isoform specific control of gene activity in vivo by the Drosophila ecdysone receptor. Mech Dev. 2003;120:909-18 pubmed
	Ghbeish N, Tsai C, Schubiger M, Zhou J, Evans R, McKeown M. The dual role of ultraspiracle, the Drosophila retinoid X receptor, in the ecdysone response. Proc Natl Acad Sci U S A. 2001;98:3867-72 pubmed
	Lee T, Marticke S, Sung C, Robinow S, Luo L. Cell-autonomous requirement of the USP/EcR-B ecdysone receptor for mushroom body neuronal remodeling in Drosophila. Neuron. 2000;28:807-18 pubmed
	Carney G, Bender M. The Drosophila ecdysone receptor (EcR) gene is required maternally for normal oogenesis. Genetics. 2000;154:1203-11 pubmed
	Mao H, Kaufman W. DNA binding properties of the ecdysteroid receptor in the salivary gland of the female ixodid tick, Amblyomma hebraeum. Insect Biochem Mol Biol. 1998;28:947-57 pubmed
	Hodin J, Riddiford L. The ecdysone receptor and ultraspiracle regulate the timing and progression of ovarian morphogenesis during Drosophila metamorphosis. Dev Genes Evol. 1998;208:304-17 pubmed
	Schubiger M, Wade A, Carney G, Truman J, Bender M. Drosophila EcR-B ecdysone receptor isoforms are required for larval molting and for neuron remodeling during metamorphosis. Development. 1998;125:2053-62 pubmed
	Talbot W, Swyryd E, Hogness D. Drosophila tissues with different metamorphic responses to ecdysone express different ecdysone receptor isoforms. Cell. 1993;73:1323-37 pubmed
	Robinow S, Talbot W, Hogness D, Truman J. Programmed cell death in the Drosophila CNS is ecdysone-regulated and coupled with a specific ecdysone receptor isoform. Development. 1993;119:1251-9 pubmed
	Truman J, Talbot W, Fahrbach S, Hogness D. Ecdysone receptor expression in the CNS correlates with stage-specific responses to ecdysteroids during Drosophila and Manduca development. Development. 1994;120:219-34 pubmed

product information

Internal ID :

820

Name :

AD4.4(EcR-B1)

Depositor Name :

Thummel, C. / Hogness, D.

Depositor Institution :

University of Utah

Depositor Website :

http://thummel.genetics.utah.edu/

Date Deposited :

11/1/01

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

ecdysone receptor (EcR-B1)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila,Sciara coprophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

8324824

Collections :

Cell signaling,Drosophila antigens,Neurodevelopment

Search Keywords :

Thummel, C. / Hogness, D., ecdysone receptor (EcR-B1), Drosophila, MIgG1, kappa light chain, Drosophila/Sciara coprophila, EcR, NR1H1, AB_2154902, monoclonal, Cell signaling/Drosophila antigens/Neurodevelopment, Immunohistochemistry/Immunofluorescence/Western Blot/Gel Supershift

Antigen Molecular Weight :

Predicted: 94 kDa; Apparent: 105 kDa

Gene :

EcR

Alternate Gene Name(s) :

NR1H1

Uniprot ID :

P34021

Antibody Registry ID :

AB_2154902

Immunogen :

TrpE-EcR-B1 fusion protein containing both isoform-specific and common segments of EcR-Bl (residues 68-878).

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 68-222

Epitope Map PubMed ID :

8324824

Recommended Applications :

Gel Supershift,Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

8324824 21507325 8119129

Immunofluorescence Pubmed IDs :

8324824 26223269 22510985 19389369 8119129 9716721 12963111 9570770 10757764 11163268 11274407 19015542

Western Blot Pubmed IDs :

8324824 9570770 10757764 14976192

Gel Supershift Pubmed IDs :

9887511 22510985 16938289

Pubmed IDs :

8306887

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 73, 1323-1337.; Cell Tissue Res. 326, 187-199.

more info or order :

Developmental Studies Hybridoma Bank product webpage