LAMP-2 | Developmental Studies Hybridoma Bank ABL-93 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

LAMP-2

catalog :

ABL-93

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

ABL-93

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation, immunohistochemistry knockout validation, immunocytochemistry knockout validation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 56

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry knockout validation; mouse; loading ...; fig 2d western blot knockout validation; mouse; loading ...; fig s2c	Hubert V, Peschel A, Langer B, Groger M, Rees A, Kain R. LAMP-2 is required for incorporating syntaxin-17 into autophagosomes and for their fusion with lysosomes. Biol Open. 2016;5:1516-1529 pubmed publisher
immunohistochemistry knockout validation; mouse; loading ...; fig 2f western blot knockout validation; mouse; loading ...; fig 6e	Rothaug M, Stroobants S, Schweizer M, Peters J, Zunke F, Allerding M, et al. LAMP-2 deficiency leads to hippocampal dysfunction but normal clearance of neuronal substrates of chaperone-mediated autophagy in a mouse model for Danon disease. Acta Neuropathol Commun. 2015;3:6 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 7a immunocytochemistry; human; 1:200; loading ...; fig 3a	Aoto K, Kato M, Akita T, Nakashima M, Mutoh H, Akasaka N, et al. ATP6V0A1 encoding the a1-subunit of the V0 domain of vacuolar H+-ATPases is essential for brain development in humans and mice. Nat Commun. 2021;12:2107 pubmed publisher
immunohistochemistry; mouse; 1:200; loading ...; fig 4g	Bassal M, Liu J, Jankowiak W, Saftig P, Bartsch U. Rapid and Progressive Loss of Multiple Retinal Cell Types in Cathepsin D-Deficient Mice-An Animal Model of CLN10 Disease. Cells. 2021;10: pubmed publisher
immunohistochemistry - paraffin section; mouse; loading ...; fig 3g	Ladinsky M, Araujo L, Zhang X, Veltri J, Galán Díez M, Soualhi S, et al. Endocytosis of commensal antigens by intestinal epithelial cells regulates mucosal T cell homeostasis. Science. 2019;363: pubmed publisher
western blot; mouse; 0.5 ug/ml; loading ...; fig s3g	Bae D, Moore K, Mella J, Hayashi S, Hollien J. Degradation of Blos1 mRNA by IRE1 repositions lysosomes and protects cells from stress. J Cell Biol. 2019;218:1118-1127 pubmed publisher
immunocytochemistry; mouse; loading ...; fig 4a	Nnah I, Wang B, Saqcena C, Weber G, Bonder E, Bagley D, et al. TFEB-driven endocytosis coordinates MTORC1 signaling and autophagy. Autophagy. 2019;15:151-164 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 5d western blot; mouse; 1:250; loading ...; fig 5b	Liu H, Jiang W, Chen X, Chang G, Zhao L, Li X, et al. Skeletal muscle-specific Sidt2 knockout in mice induced muscular dystrophy-like phenotype. Metabolism. 2018;85:259-270 pubmed publisher
immunocytochemistry; mouse; loading ...; fig 3b western blot; mouse; loading ...; fig 3c	Kissing S, Rudnik S, Damme M, Lüllmann Rauch R, Ichihara A, Kornak U, et al. Disruption of the vacuolar-type H+-ATPase complex in liver causes MTORC1-independent accumulation of autophagic vacuoles and lysosomes. Autophagy. 2017;13:670-685 pubmed publisher
immunocytochemistry; mouse; 1:200; loading ...; fig 2b	Rahman N, Ramos Espiritu L, Milner T, Buck J, Levin L. Soluble adenylyl cyclase is essential for proper lysosomal acidification. J Gen Physiol. 2016;148:325-39 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:50; loading ...; fig 5i	Hubbs A, Fluharty K, Edwards R, Barnabei J, Grantham J, Palmer S, et al. Accumulation of Ubiquitin and Sequestosome-1 Implicate Protein Damage in Diacetyl-Induced Cytotoxicity. Am J Pathol. 2016;186:2887-2908 pubmed publisher
immunocytochemistry; mouse; 1:200; loading ...; fig s7a	Demetriades C, Plescher M, Teleman A. Lysosomal recruitment of TSC2 is a universal response to cellular stress. Nat Commun. 2016;7:10662 pubmed publisher
immunocytochemistry; mouse; fig 5	Plescher M, Teleman A, Demetriades C. TSC2 mediates hyperosmotic stress-induced inactivation of mTORC1. Sci Rep. 2015;5:13828 pubmed publisher
immunocytochemistry; mouse	Omari S, Waters M, Naranian T, Kim K, Perumalsamy A, Chi M, et al. Mcl-1 is a key regulator of the ovarian reserve. Cell Death Dis. 2015;6:e1755 pubmed publisher
immunocytochemistry; mouse western blot; mouse; 1:1000	Kett L, Stiller B, Bernath M, Tasset I, Blesa J, Jackson Lewis V, et al. Î±-Synuclein-independent histopathological and motor deficits in mice lacking the endolysosomal Parkinsonism protein Atp13a2. J Neurosci. 2015;35:5724-42 pubmed publisher
western blot; rat; 1:1000	Liu S, Sarkar C, Dinizo M, Faden A, Koh E, Lipinski M, et al. Disrupted autophagy after spinal cord injury is associated with ER stress and neuronal cell death. Cell Death Dis. 2015;6:e1582 pubmed publisher
western blot; mouse; 1:1000; fig 4	Sarkar C, Zhao Z, Aungst S, Sabirzhanov B, Faden A, Lipinski M. Impaired autophagy flux is associated with neuronal cell death after traumatic brain injury. Autophagy. 2014;10:2208-22 pubmed publisher
western blot; human; fig 5	Voss M, KÃ¼nzel U, Higel F, Kuhn P, Colombo A, Fukumori A, et al. Shedding of glycan-modifying enzymes by signal peptide peptidase-like 3 (SPPL3) regulates cellular N-glycosylation. EMBO J. 2014;33:2890-905 pubmed publisher
immunocytochemistry; mouse; 1:200; fig 3	Yu S, Yehia G, Wang J, Stypulkowski E, Sakamori R, Jiang P, et al. Global ablation of the mouse Rab11a gene impairs early embryogenesis and matrix metalloproteinase secretion. J Biol Chem. 2014;289:32030-43 pubmed publisher
immunocytochemistry; mouse; fig 1	Rajapakshe A, Podyma Inoue K, Terasawa K, Hasegawa K, Namba T, Kumei Y, et al. Lysosome-associated membrane proteins (LAMPs) regulate intracellular positioning of mitochondria in MC3T3-E1 cells. Exp Cell Res. 2015;331:211-22 pubmed publisher
immunocytochemistry; mouse; fig 5d	Schneppenheim J, Hüttl S, Mentrup T, Lüllmann Rauch R, Rothaug M, Engelke M, et al. The intramembrane proteases signal Peptide peptidase-like 2a and 2b have distinct functions in vivo. Mol Cell Biol. 2014;34:1398-411 pubmed publisher
immunohistochemistry; human western blot; human	Lee T, Streb J, Georger M, Miano J. Tissue expression of the novel serine carboxypeptidase Scpep1. J Histochem Cytochem. 2006;54:701-11 pubmed
	Joshi B, Youssef S, Bron R, de Bruin A, Kampinga H, Zuhorn I. DNAJB6b-enriched small extracellular vesicles decrease polyglutamine aggregation in in vitro and in vivo models of Huntington disease. iScience. 2021;24:103282 pubmed publisher
	Dong T, Tejwani L, Jung Y, Kokubu H, Luttik K, Driessen T, et al. Microglia regulate brain progranulin levels through the endocytosis/lysosomal pathway. JCI Insight. 2021;6: pubmed publisher
	Lancaster C, Fountain A, Dayam R, Somerville E, Sheth J, Jacobelli V, et al. Phagosome resolution regenerates lysosomes and maintains the degradative capacity in phagocytes. J Cell Biol. 2021;220: pubmed publisher
	Clayton E, Milioto C, Muralidharan B, Norona F, Edgar J, Soriano A, et al. Frontotemporal dementia causative CHMP2B impairs neuronal endolysosomal traffic-rescue by TMEM106B knockdown. Brain. 2018;141:3428-3442 pubmed publisher
	Sobajima T, Yoshimura S, Maeda T, Miyata H, Miyoshi E, Harada A. The Rab11-binding protein RELCH/KIAA1468 controls intracellular cholesterol distribution. J Cell Biol. 2018;217:1777-1796 pubmed publisher
	Okuda K, Tong M, Dempsey B, Moore K, Gazzinelli R, Silverman N. Leishmania amazonensis Engages CD36 to Drive Parasitophorous Vacuole Maturation. PLoS Pathog. 2016;12:e1005669 pubmed publisher
	Clayton E, Mizielinska S, Edgar J, Nielsen T, Marshall S, Norona F, et al. Frontotemporal dementia caused by CHMP2B mutation is characterised by neuronal lysosomal storage pathology. Acta Neuropathol. 2015;130:511-23 pubmed publisher
	Pei G, Schnettger L, Bronietzki M, Repnik U, Griffiths G, Gutierrez M. Interferon-Î³-inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages. Mol Biol Cell. 2015;26:3061-70 pubmed publisher
	Sobajima T, Yoshimura S, Iwano T, Kunii M, Watanabe M, Atik N, et al. Rab11a is required for apical protein localisation in the intestine. Biol Open. 2014;4:86-94 pubmed publisher
	Castillo K, Valenzuela V, Matus S, Nassif M, Onate M, Fuentealba Y, et al. Measurement of autophagy flux in the nervous system in vivo. Cell Death Dis. 2013;4:e917 pubmed publisher
	Sato T, Iwano T, Kunii M, Matsuda S, Mizuguchi R, Jung Y, et al. Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis. J Cell Sci. 2014;127:422-31 pubmed publisher
	Stewart R, Teng H, Wilkinson R. "Late" macroendosomes and acidic endosomes in vertebrate motor nerve terminals. J Comp Neurol. 2012;520:4275-93 pubmed publisher
	Nagajyothi F, Weiss L, Silver D, Desruisseaux M, Scherer P, Herz J, et al. Trypanosoma cruzi utilizes the host low density lipoprotein receptor in invasion. PLoS Negl Trop Dis. 2011;5:e953 pubmed publisher
	Schneede A, Schmidt C, Hölttä Vuori M, Heeren J, Willenborg M, Blanz J, et al. Role for LAMP-2 in endosomal cholesterol transport. J Cell Mol Med. 2011;15:280-95 pubmed publisher
	Ferguson C, Lenk G, Meisler M. Defective autophagy in neurons and astrocytes from mice deficient in PI(3,5)P2. Hum Mol Genet. 2009;18:4868-78 pubmed publisher
	Guo X, Tu L, Gumper I, Plesken H, Novak E, Chintala S, et al. Involvement of vps33a in the fusion of uroplakin-degrading multivesicular bodies with lysosomes. Traffic. 2009;10:1350-61 pubmed publisher
	Diril M, Schmidt S, Krauss M, Gawlik V, Joost H, Schurmann A, et al. Lysosomal localization of GLUT8 in the testis--the EXXXLL motif of GLUT8 is sufficient for its intracellular sorting via AP1- and AP2-mediated interaction. FEBS J. 2009;276:3729-43 pubmed publisher
	Holt O, Kanno E, Bossi G, Booth S, Daniele T, Santoro A, et al. Slp1 and Slp2-a localize to the plasma membrane of CTL and contribute to secretion from the immunological synapse. Traffic. 2008;9:446-57 pubmed publisher
	Miedel M, Weixel K, Bruns J, Traub L, Weisz O. Posttranslational cleavage and adaptor protein complex-dependent trafficking of mucolipin-1. J Biol Chem. 2006;281:12751-9 pubmed
	Fang D, Kerppola T. Ubiquitin-mediated fluorescence complementation reveals that Jun ubiquitinated by Itch/AIP4 is localized to lysosomes. Proc Natl Acad Sci U S A. 2004;101:14782-7 pubmed
	Esselens C, Oorschot V, Baert V, Raemaekers T, Spittaels K, Serneels L, et al. Presenilin 1 mediates the turnover of telencephalin in hippocampal neurons via an autophagic degradative pathway. J Cell Biol. 2004;166:1041-54 pubmed
	Frackowiak J, Sukontasup T, Potempska A, Mazur Kolecka B. Lysosomal deposition of Abeta in cultures of brain vascular smooth muscle cells is enhanced by iron. Brain Res. 2004;1002:67-75 pubmed
	Moreno R. Differential expression of lysosomal associated membrane protein (LAMP-1) during mammalian spermiogenesis. Mol Reprod Dev. 2003;66:202-9 pubmed
	Tanaka Y, Guhde G, Suter A, Eskelinen E, Hartmann D, Lullmann Rauch R, et al. Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice. Nature. 2000;406:902-6 pubmed
	Lichter Konecki U, Moter S, Krawisz B, Schlotter M, Hipke C, Konecki D. Expression patterns of murine lysosome-associated membrane protein 2 (Lamp-2) transcripts during morphogenesis. Differentiation. 1999;65:43-58 pubmed
	Li R, Blanchette Mackie E, Ladisch S. Induction of endocytic vesicles by exogenous C(6)-ceramide. J Biol Chem. 1999;274:21121-7 pubmed
	Braulke T, Dittmer F, Gotz W, Von Figura K. Alteration in pancreatic immunoreactivity of insulin-like growth factor (IGF)-binding protein (IGFBP)-6 and in intracellular degradation of IGFBP-3 in fibroblasts of IGF-II receptor/IGF-II-deficient mice. Horm Metab Res. 1999;31:235-41 pubmed
	Andrejewski N, Punnonen E, Guhde G, Tanaka Y, Lullmann Rauch R, Hartmann D, et al. Normal lysosomal morphology and function in LAMP-1-deficient mice. J Biol Chem. 1999;274:12692-701 pubmed
	Ramachandra L, Sramkoski R, Canaday D, Boom W, Harding C. Flow analysis of MHC molecules and other membrane proteins in isolated phagosomes. J Immunol Methods. 1998;213:53-71 pubmed
	Jasiulionis M, Chammas R, Ventura A, Travassos L, Brentani R. alpha6beta1-Integrin, a major cell surface carrier of beta1-6-branched oligosaccharides, mediates migration of EJ-ras-transformed fibroblasts on laminin-1 independently of its glycosylation state. Cancer Res. 1996;56:1682-9 pubmed
	Goldenthal K, Hedman K, Chen J, August J, Willingham M. Postfixation detergent treatment for immunofluorescence suppresses localization of some integral membrane proteins. J Histochem Cytochem. 1985;33:813-20 pubmed
	Chen J, Chen G, D Souza M, Murphy T, August J. Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2. Biochem Soc Symp. 1986;51:97-112 pubmed
	Chen J, Murphy T, Willingham M, Pastan I, August J. Identification of two lysosomal membrane glycoproteins. J Cell Biol. 1985;101:85-95 pubmed
	Amos B, Lotan R. Modulation of lysosomal-associated membrane glycoproteins during retinoic acid-induced embryonal carcinoma cell differentiation. J Biol Chem. 1990;265:19192-8 pubmed

product information

Internal ID :

479

Name :

ABL-93

Depositor Name :

August, J.T.

Depositor Institution :

Johns Hopkins University School of Medicine

Date Deposited :

8/1/86

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

LAMP-2

Antigen Species :

Mouse

Host Species :

rat

Isotype :

RIgG2a

Isotype for catalog (legacy) :

IgG2a

Positive Tested Species Reactivity :

Mouse

Species Tested (legacy) :

mouse

Initial Publication Pubmed ID :

2409098

Collections :

CD antigens,Cell adhesion,Cell markers,Cell signaling

Search Keywords :

August, J.T., LAMP-2 (110 kDa lysosomal membrane glycoprotein), Mouse, RIgG2a, Mouse, Lamp2, CD107b, Mac3, LGP-B, AB_2134767, monoclonal, Cell signaling/Cluster Determinant antigens/Cell markers/Cell adhesion, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/FACS

Antigen Molecular Weight :

Predicted: 43 kDa; Apparent: around 110 kDa

Gene :

Lamp2

Alternate Gene Name(s) :

CD107b, Mac3, LGP-B, Igp110

Uniprot ID :

P17047

Antibody Registry ID :

AB_2134767

Synonyms (Alternate Clone Names) :

CD107b

Immunogen :

BALB/c 3T3 mouse embryo fibroblast tissue culture cell glycoproteins purified by lectin chromatography

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3x63-Ag8-653

Epitope Mapped :

Yes

Epitope Location or Sequence :

Luminal region of LAMP-2

Epitope Map PubMed ID :

10448712

Recommended Applications :

FACS,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

10448712 25855184

Immunofluorescence Pubmed IDs :

3894499 2409098 14988035 10212251 10409665 15469925 22740045 12950108 16517607 19566896

Western Blot Pubmed IDs :

19793721 10212251 2172246 12950108 10972293 19566896

Immunoprecipitation Pubmed IDs :

2409098 10212251 10226807

FACS Pubmed IDs :

9671125

Pubmed IDs :

3101702 8603420

Additional Information :

LAMP2 is a marker of lysosmes and late endosomes. ABL-93 is directed against the luminal domain of LAMP-2 common to all isoforms. RRID: AB_2134767

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 101, 85-95.; Arch. Biochem. Biophys. 239, 574-586.; Biochem. Soc. Symp. 51, 97-112.; J. Tiss. Cult. Meth. 14, 31-36.; Cancer Res. 56, 1682-1689.; Brain Res. 1002, 67-75.; Hum. Mole. Gen. 18(24), 4868-4878.

more info or order :

Developmental Studies Hybridoma Bank product webpage