tubulin (alpha-) | Developmental Studies Hybridoma Bank AA4.3 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

tubulin (alpha-)

catalog :

AA4.3

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AA4.3

reactivity :

Naegleria gruberi strain NEG-M, human, rat, fruit fly

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 26

Published Application/Species/Sample/Dilution	Reference
western blot; human; 0.3 ug/ml; loading ...; fig 5c	Scott C, Marsden A, Rebagliati M, Zhang Q, Chamling X, Searby C, et al. Nuclear/cytoplasmic transport defects in BBS6 underlie congenital heart disease through perturbation of a chromatin remodeling protein. PLoS Genet. 2017;13:e1006936 pubmed publisher
western blot; rat; 1:4000	Bernard L, Zhang H. MARK/Par1 Kinase Is Activated Downstream of NMDA Receptors through a PKA-Dependent Mechanism. PLoS ONE. 2015;10:e0124816 pubmed publisher
western blot; fruit fly; 1:1000	Nagy P, Kárpáti M, Varga A, Pircs K, Venkei Z, Takáts S, et al. Atg17/FIP200 localizes to perilysosomal Ref(2)P aggregates and promotes autophagy by activation of Atg1 in Drosophila. Autophagy. 2014;10:453-67 pubmed publisher
	Oh K, Haney J, Wang X, Chuang C, Richmond J, Kim H. ERG-28 controls BK channel trafficking in the ER to regulate synaptic function and alcohol response in C. elegans. elife. 2017;6: pubmed publisher
	Hofherr A, Wagner C, Watnick T, KÃ¶ttgen M. Targeted rescue of a polycystic kidney disease mutation by lysosomal inhibition. Kidney Int. 2016;89:949-55 pubmed publisher
	Lee J, Lee S, Chen C, Shim H, Kim Ha J. shot regulates the microtubule reorganization required for localization of axis-determining mRNAs during oogenesis. FEBS Lett. 2016;590:431-44 pubmed publisher
	Nechiporuk T, MCGANN J, Mullendorff K, Hsieh J, Wurst W, Floss T, et al. The REST remodeling complex protects genomic integrity during embryonic neurogenesis. elife. 2016;5:e09584 pubmed publisher
	Mi Mi L, Pruyne D. Loss of Sarcomere-associated Formins Disrupts Z-line Organization, but does not Prevent Thin Filament Assembly in Caenorhabditis elegans Muscle. J Cytol Histol. 2015;6: pubmed
	Brockmann B, Mastel H, Oswald F, Maier D. Analysis of the interaction between human RITA and Drosophila Suppressor of Hairless. Hereditas. 2014;151:209-19 pubmed publisher
	Ramdas N, Shivashankar G. Cytoskeletal control of nuclear morphology and chromatin organization. J Mol Biol. 2015;427:695-706 pubmed publisher
	Sen A, Damm V, Cox R. Drosophila clueless is highly expressed in larval neuroblasts, affects mitochondrial localization and suppresses mitochondrial oxidative damage. PLoS ONE. 2013;8:e54283 pubmed publisher
	Pircs K, Nagy P, Varga A, Venkei Z, Erdi B, Hegedus K, et al. Advantages and limitations of different p62-based assays for estimating autophagic activity in Drosophila. PLoS ONE. 2012;7:e44214 pubmed publisher
	Walsh C. The structure of the mitotic spindle and nucleolus during mitosis in the amebo-flagellate Naegleria. PLoS ONE. 2012;7:e34763 pubmed publisher
	Zhang W, Peng G, Lin S, Zhang P. DNA damage response is suppressed by the high cyclin-dependent kinase 1 activity in mitotic mammalian cells. J Biol Chem. 2011;286:35899-905 pubmed publisher
	Lee N, Skinner J, Zajac J, MacLean H. Ornithine decarboxylase is upregulated by the androgen receptor in skeletal muscle and regulates myoblast proliferation. Am J Physiol Endocrinol Metab. 2011;301:E172-9 pubmed publisher
	Kim D, Lee B, Suh E. Ionizing radiation-induced TAp63? phosphorylation at C-terminal S/TQ motifs requires the N-terminal transactivation (TA) domain. Cell Cycle. 2011;10:840-9 pubmed
	Molina M, Neto A, Maeso I, Gomez Skarmeta J, Salo E, Cebria F. Noggin and noggin-like genes control dorsoventral axis regeneration in planarians. Curr Biol. 2011;21:300-5 pubmed publisher
	Fukumoto H, Takahashi H, Tarui N, Matsui J, Tomita T, Hirode M, et al. A noncompetitive BACE1 inhibitor TAK-070 ameliorates Abeta pathology and behavioral deficits in a mouse model of Alzheimer's disease. J Neurosci. 2010;30:11157-66 pubmed publisher
	Larson S, Lee H, Hung P, Matthews L, Robinson D, Evans J. Cortical mechanics and meiosis II completion in mammalian oocytes are mediated by myosin-II and Ezrin-Radixin-Moesin (ERM) proteins. Mol Biol Cell. 2010;21:3182-92 pubmed publisher
	Li X, Urwyler O, Suter B. Drosophila Xpd regulates Cdk7 localization, mitotic kinase activity, spindle dynamics, and chromosome segregation. PLoS Genet. 2010;6:e1000876 pubmed publisher
	Schleich S, Teleman A. Akt phosphorylates both Tsc1 and Tsc2 in Drosophila, but neither phosphorylation is required for normal animal growth. PLoS ONE. 2009;4:e6305 pubmed publisher
	Mohammad M, Raftos D, Joss J. Cytoskeletal proteins in thymic epithelial cells of the Australian lungfish Neoceratodus forsteri. J Anat. 2009;214:140-52 pubmed publisher
	Larsen K, Xu J, Cermelli S, Shu Z, Gross S. BicaudalD actively regulates microtubule motor activity in lipid droplet transport. PLoS ONE. 2008;3:e3763 pubmed publisher
	Mar J, Walsh C. Temperature-shock induction of multiple flagella induces additional synthesis of flagellum specific mRNAs and tubulin. Exp Cell Res. 2008;314:896-902 pubmed publisher
	Walsh C. Synthesis and assembly of the cytoskeleton of Naegleria gruberi flagellates. J Cell Biol. 1984;98:449-56 pubmed
	Shea D, Walsh C. mRNAs for alpha- and beta-tubulin and flagellar calmodulin are among those coordinately regulated when Naegleria gruberi amebae differentiate into flagellates. J Cell Biol. 1987;105:1303-9 pubmed

product information

Internal ID :

939

Name :

AA4.3

Depositor Name :

Walsh, C.

Depositor Institution :

University of Pittsburgh

Depositor Website :

http://www.biology.pitt.edu/person/charles-walsh

Date Deposited :

12/20/05

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

tubulin (alpha-)

Antigen Species :

Naegleria gruberi

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Broad species,C. elegans,Drosophila melanogaster,Fish,Human,Mammal,Mouse,Plant,Protist,Rat,Worm

Species Tested (legacy) :

very wide; plants, protists, animals (mammals, worms etc.)

Initial Publication Pubmed ID :

6363422 3654753

Collections :

C. elegans,Cytoskeleton,Drosophila antigens,Human,Microbe antibodies,Muscular dystrophy,Xenopus

Search Keywords :

Walsh, C., tubulin (alpha-), Naegleria gruberi, MIgG1, kappa light chain , Broad species/Plant/protist/Mammal/Mouse/Rat/Human/Worm/Fish/Drosophila melanogaster/C. elegans, TBA1, , AB_579793, monoclonal, C. elegans/Cytoskeletal Microbe antibodies/Xenopus/Drosophila antigens/Human/Muscular Dystrophy , Immunohistochemistry/Immunohistochemistry/Western Blot/Immunoprecipitation/ELISA

Antigen Molecular Weight :

Predicted: 50 kDa; Apparent: 55 kDa

Gene :

TBA1

Uniprot ID :

P11237

Antibody Registry ID :

AB_579793

Synonyms (Alternate Clone Names) :

AA-4.3

Immunogen :

flagellar axoneme

Immunogen Sequence :

Total protein

Clonality :

Monoclonal

Myeloma Strain :

P3-X63Ag8

Epitope Mapped :

Recommended Applications :

ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

19166477

Immunofluorescence Pubmed IDs :

6363422 21878640 18201698 21295481 22493714 19018277 25281900 25588307 26832192

Western Blot Pubmed IDs :

6363422 3654753 21878640 18201698 21505150 22952930 26161293 21325887 26924047 19609361 20720123 31308408

ELISA Pubmed IDs :

6363422

Pubmed IDs :

20660156 23342118 20300654 20660156

Additional Information :

Initial characterization [PMID 6363422] performed on Naegleria gruberi tubulins suggested that this antibody target was beta-tubulin. Further characterization [PMID 3654753] demonstrated that the target was alpha-tubulin. This antibody may recognize more than one alpha-tubulin isoform. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 98, 449-456. (This original description of AA4.3 is wrong; it is against a-tubulin not b-tubulin.); J. Cell Biol. 105, 1303-1309.; Mole. Biol. Cell 21, 3182-3192.; J. Biol. Chem. 286(41), 35899-35905. doi: 10.1074/jbc.M111.267690.

more info or order :

Developmental Studies Hybridoma Bank product webpage