tubulin (beta-) | Developmental Studies Hybridoma Bank AA12.1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

tubulin (beta-)

catalog :

AA12.1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AA12.1

reactivity :

Naegleria gruberi strain NEG-M, human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
western blot; human	Makowski S, Wang Z, Pomerantz J. A protease-independent function for SPPL3 in NFAT activation. Mol Cell Biol. 2015;35:451-67 pubmed publisher
western blot; mouse	Prosser B, Ward C, Lederer W. X-ROS signaling: rapid mechano-chemo transduction in heart. Science. 2011;333:1440-5 pubmed publisher
	Fritz Laylin L, Levy Y, Levitan E, Chen S, Cande W, Lai E, et al. Rapid centriole assembly in Naegleria reveals conserved roles for both de novo and mentored assembly. Cytoskeleton (Hoboken). 2016;73:109-16 pubmed publisher
	Hamblet C, Makowski S, Tritapoe J, Pomerantz J. NK Cell Maturation and Cytotoxicity Are Controlled by the Intramembrane Aspartyl Protease SPPL3. J Immunol. 2016;196:2614-26 pubmed publisher
	Eichinger J, Satterlie R. Organization of the ectodermal nervous structures in medusae: cubomedusae. Biol Bull. 2014;226:41-55 pubmed
	Kombairaju P, Kerr J, Roche J, Pratt S, Lovering R, Sussan T, et al. Genetic silencing of Nrf2 enhances X-ROS in dysferlin-deficient muscle. Front Physiol. 2014;5:57 pubmed publisher
	Sen A, Damm V, Cox R. Drosophila clueless is highly expressed in larval neuroblasts, affects mitochondrial localization and suppresses mitochondrial oxidative damage. PLoS ONE. 2013;8:e54283 pubmed publisher
	Farrell J, O Farrell P. Mechanism and regulation of Cdc25/Twine protein destruction in embryonic cell-cycle remodeling. Curr Biol. 2013;23:118-26 pubmed publisher
	Whitcomb D, Larusch J, Krasinskas A, Klei L, Smith J, Brand R, et al. Common genetic variants in the CLDN2 and PRSS1-PRSS2 loci alter risk for alcohol-related and sporadic pancreatitis. Nat Genet. 2012;44:1349-54 pubmed publisher
	Khairallah R, Shi G, Sbrana F, Prosser B, Borroto C, Mazaitis M, et al. Microtubules underlie dysfunction in duchenne muscular dystrophy. Sci Signal. 2012;5:ra56 pubmed publisher
	Abdou M, Peng C, Huang J, Zyaan O, Wang S, Li S, et al. Wnt signaling cross-talks with JH signaling by suppressing Met and gce expression. PLoS ONE. 2011;6:e26772 pubmed publisher
	Huang J, Tian L, Peng C, Abdou M, Wen D, Wang Y, et al. DPP-mediated TGFbeta signaling regulates juvenile hormone biosynthesis by activating the expression of juvenile hormone acid methyltransferase. Development. 2011;138:2283-91 pubmed publisher
	Dhaunchak A, Huang J, De Faria Junior O, Roth A, Pedraza L, Antel J, et al. A proteome map of axoglial specializations isolated and purified from human central nervous system. Glia. 2010;58:1949-60 pubmed publisher
	Li X, Urwyler O, Suter B. Drosophila Xpd regulates Cdk7 localization, mitotic kinase activity, spindle dynamics, and chromosome segregation. PLoS Genet. 2010;6:e1000876 pubmed publisher
	Mar J, Walsh C. Temperature-shock induction of multiple flagella induces additional synthesis of flagellum specific mRNAs and tubulin. Exp Cell Res. 2008;314:896-902 pubmed publisher
	Walsh C. Synthesis and assembly of the cytoskeleton of Naegleria gruberi flagellates. J Cell Biol. 1984;98:449-56 pubmed
	Shea D, Walsh C. mRNAs for alpha- and beta-tubulin and flagellar calmodulin are among those coordinately regulated when Naegleria gruberi amebae differentiate into flagellates. J Cell Biol. 1987;105:1303-9 pubmed

product information

Internal ID :

927

Name :

AA12.1

Depositor Name :

Walsh, C.

Depositor Institution :

University of Pittsburgh

Depositor Website :

http://www.biology.pitt.edu/person/charles-walsh

Date Deposited :

12/20/05

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

tubulin (beta-)

Antigen Species :

Naegleria gruberi

Host Species :

mouse

Isotype :

MIgM, kappa light chain

Isotype for catalog (legacy) :

IgM, kappa light chain

Positive Tested Species Reactivity :

Broad species,Drosophila melanogaster,Human,Mammal,Mouse,Protist,Rat

Species Tested (legacy) :

protists, mammals, not further tested

Initial Publication Pubmed ID :

6363422 3654753

Collections :

C. elegans,Cytoskeleton,Drosophila antigens,Human,Microbe antibodies,Muscular dystrophy,Xenopus

Search Keywords :

Walsh, C., tubulin (beta-), Naegleria gruberi, MIgM, kappa light chain , Broad species/protist/Mammal/Mouse/Rat/Human/Drosophila melanogaster, TBB, , AB_579794, monoclonal, C. elegans/Cytoskeletal Microbe antibodies/Xenopus/Drosophila antigens/Human/Muscular Dystrophy , Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/ELISA

Antigen Molecular Weight :

Predicted: 50 kDa; Apparent: 55 kDa

Gene :

TBB

Uniprot ID :

P34108

Antibody Registry ID :

AB_579794

Synonyms (Alternate Clone Names) :

AA-12.1

Immunogen :

flagellar axoneme

Immunogen Sequence :

Total protein

Clonality :

Monoclonal

Myeloma Strain :

P3-X63Ag8

Epitope Mapped :

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

24648206

Western Blot Pubmed IDs :

3654753 20830807 22871609 18201698 24600403 23143602 21558376 22087234 26851218 25384971

ELISA Pubmed IDs :

6363422

Pubmed IDs :

23342118 23290551 26873879 20300654

Additional Information :

This antibody may recognize more than one beta-tubulin isoform. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 98, 449-456. (This original description of AA12.1 is wrong; it is against b-tubulin not a-tubulin.); J. Cell Biol. 105, 1303-1309.

more info or order :

Developmental Studies Hybridoma Bank product webpage