Myosin heavy chain (human slow fibers) | Developmental Studies Hybridoma Bank A4.951 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Myosin heavy chain (human slow fibers)

catalog :

A4.951

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A4.951

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 29

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; rat; 1:250; loading ...; fig 2a, 2b	Giacco A, delli Paoli G, Simiele R, Caterino M, Ruoppolo M, Bloch W, et al. Exercise with food withdrawal at thermoneutrality impacts fuel use, the microbiome, AMPK phosphorylation, muscle fibers, and thyroid hormone levels in rats. Physiol Rep. 2020;8:e14354 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:50; fig 2	Barone R, Macaluso F, Sangiorgi C, Campanella C, Marino Gammazza A, Moresi V, et al. Skeletal muscle Heat shock protein 60 increases after endurance training and induces peroxisome proliferator-activated receptor gamma coactivator 1 Î±1 expression. Sci Rep. 2016;6:19781 pubmed publisher
immunohistochemistry; human	Karlsen A, CouppÃ© C, Andersen J, Mikkelsen U, Nielsen R, Magnusson S, et al. Matters of fiber size and myonuclear domain: Does size matter more than age?. Muscle Nerve. 2015;52:1040-6 pubmed publisher
immunohistochemistry - frozen section; human	Snijders T, Verdijk L, Smeets J, McKay B, Senden J, Hartgens F, et al. The skeletal muscle satellite cell response to a single bout of resistance-type exercise is delayed with aging in men. Age (Dordr). 2014;36:9699 pubmed publisher
immunohistochemistry; human	Janbaz A, Lindström M, Liu J, Pedrosa Domellöf F. Intermediate filaments in the human extraocular muscles. Invest Ophthalmol Vis Sci. 2014;55:5151-9 pubmed publisher
	Lessard S, Macdonald T, Pathak P, Han M, Coffey V, Edge J, et al. JNK regulates muscle remodeling via myostatin/SMAD inhibition. Nat Commun. 2018;9:3030 pubmed publisher
	Nederveen J, Joanisse S, Snijders T, Thomas A, Kumbhare D, Parise G. The influence of capillarization on satellite cell pool expansion and activation following exercise-induced muscle damage in healthy young men. J Physiol. 2018;596:1063-1078 pubmed publisher
	Tjust A, Danielsson A, Andersen P, Brannstrom T, Pedrosa Domellöf F. Impact of Amyotrophic Lateral Sclerosis on Slow Tonic Myofiber Composition in Human Extraocular Muscles. Invest Ophthalmol Vis Sci. 2017;58:3708-3715 pubmed publisher
	Jarosch I, Gehlert S, Jacko D, Koczulla R, Wencker M, Welte T, et al. Different Training-Induced Skeletal Muscle Adaptations in COPD Patients with and without Alpha-1 Antitrypsin Deficiency. Respiration. 2016;92:339-347 pubmed
	Choi Y, Chen P, Shin S, Zhang J, Hwang S, Lee K. Mild heat stress enhances differentiation and proliferation of Japanese quail myoblasts and enhances slow muscle fiber characteristics. Poult Sci. 2016;95:1912-7 pubmed publisher
	Mackey A, Rasmussen L, Kadi F, Schjerling P, Helmark I, Ponsot E, et al. Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication. FASEB J. 2016;30:2266-81 pubmed publisher
	Joanisse S, McKay B, Nederveen J, Scribbans T, Gurd B, Gillen J, et al. Satellite cell activity, without expansion, after nonhypertrophic stimuli. Am J Physiol Regul Integr Comp Physiol. 2015;309:R1101-11 pubmed publisher
	Molsted S, Andersen J, Harrison A, Eidemak I, Mackey A. Fiber type-specific response of skeletal muscle satellite cells to high-intensity resistance training in dialysis patients. Muscle Nerve. 2015;52:736-45 pubmed publisher
	Hollinger K, Shanely R, Quindry J, Selsby J. Long-term quercetin dietary enrichment decreases muscle injury in mdx mice. Clin Nutr. 2015;34:515-22 pubmed publisher
	Osterlund C, Liu J, Thornell L, Eriksson P. Intrafusal myosin heavy chain expression of human masseter and biceps muscles at young age shows fundamental similarities but also marked differences. Histochem Cell Biol. 2013;139:895-907 pubmed publisher
	Keizer H, Schaart G, Tandon N, Glatz J, Luiken J. Subcellular immunolocalisation of fatty acid translocase (FAT)/CD36 in human type-1 and type-2 skeletal muscle fibres. Histochem Cell Biol. 2004;121:101-7 pubmed
	Liu J, Thornell L, Pedrosa Domellöf F. Muscle spindles in the deep muscles of the human neck: a morphological and immunocytochemical study. J Histochem Cytochem. 2003;51:175-86 pubmed
	Liu J, Eriksson P, Thornell L, Pedrosa Domellöf F. Myosin heavy chain composition of muscle spindles in human biceps brachii. J Histochem Cytochem. 2002;50:171-83 pubmed
	Maggs A, Taylor Harris P, Peckham M, Hughes S. Evidence for differential post-translational modifications of slow myosin heavy chain during murine skeletal muscle development. J Muscle Res Cell Motil. 2000;21:101-13 pubmed
	Pedrosa Domellof F, Holmgren Y, Lucas C, Hoh J, Thornell L. Human extraocular muscles: unique pattern of myosin heavy chain expression during myotube formation. Invest Ophthalmol Vis Sci. 2000;41:1608-16 pubmed
	Shiotani A, Westra W, Flint P. Myosin heavy chain composition in human laryngeal muscles. Laryngoscope. 1999;109:1521-4 pubmed
	Levine S, Kaiser L, Leferovich J, Tikunov B. Cellular adaptations in the diaphragm in chronic obstructive pulmonary disease. N Engl J Med. 1997;337:1799-806 pubmed
	Dunn S, Michel R. Coordinated expression of myosin heavy chain isoforms and metabolic enzymes within overloaded rat muscle fibers. Am J Physiol. 1997;273:C371-83 pubmed
	Tikunov B, Levine S, Mancini D. Chronic congestive heart failure elicits adaptations of endurance exercise in diaphragmatic muscle. Circulation. 1997;95:910-6 pubmed
	Tikunov B, Mancini D, Levine S. Changes in myofibrillar protein composition of human diaphragm elicited by congestive heart failure. J Mol Cell Cardiol. 1996;28:2537-41 pubmed
	Michel R, Parry D, Dunn S. Regulation of myosin heavy chain expression in adult rat hindlimb muscles during short-term paralysis: comparison of denervation and tetrodotoxin-induced neural inactivation. FEBS Lett. 1996;391:39-44 pubmed
	Cho M, Webster S, Blau H. Evidence for myoblast-extrinsic regulation of slow myosin heavy chain expression during muscle fiber formation in embryonic development. J Cell Biol. 1993;121:795-810 pubmed
	Hughes S, Cho M, Karsch Mizrachi I, Travis M, Silberstein L, Leinwand L, et al. Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle. Dev Biol. 1993;158:183-99 pubmed
	Webster C, Silberstein L, Hays A, Blau H. Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy. Cell. 1988;52:503-13 pubmed

product information

Internal ID :

1280

Name :

A4.951

Depositor Name :

Blau, H.M.

Depositor Institution :

Baxter Lab for Stem Cell Biology, Stanford University

Depositor Website :

http://baxterlab.stanford.edu/

Date Deposited :

8/25/93

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Myosin heavy chain (human slow fibers)

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Fish,Human,Quail,Rodent

Species Tested (legacy) :

human, rodent (others?)

Initial Publication Pubmed ID :

3342447

Depositor Notes (Special Instructions) :

Fusion: 1983. Specific for slow fibers.

Collections :

Cardiac development,Cell markers,Cytoskeleton,Human,Skeletal muscle

Search Keywords :

Blau, H. M., Myosin heavy chain (human slow fibers), Human, MIgG1, kappa light chain, Human/Rodent/Quail/Fish, MYH7, CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB, AB_528385, monoclonal, Human/Cell markers/Cytoskeletal elements/Skeletal muscle/Cardiac development proteins, Immunohistochemistry/Immunofluorescence/Western Blot/ELISA//Immunoprecipitation

Antigen Molecular Weight :

220kDa

Gene :

MYH7

Alternate Gene Name(s) :

CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB

Uniprot ID :

P12883

Antibody Registry ID :

AB_528385

Immunogen :

partially purified myosin (pyrophosphate extracted) from human adult (22 year old) skeletal muscle

Clonality :

Monoclonal

Myeloma Strain :

653

Epitope Mapped :

Yes

Epitope Location or Sequence :

N-terminal first 100 aa

Epitope Map PubMed ID :

7687223

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

8491773 10845576 11799136 12533526 14740222 10961835 9277335 23306907 27686000

Immunofluorescence Pubmed IDs :

3342447 25736589 24998094 26936358 26333785 10961835 7687223 8706926 29315567

Western Blot Pubmed IDs :

7687223 3342447 8491773 10845576 11799136 27038421 9054750 10499066 9004169 9400036

ELISA Pubmed IDs :

7687223 3342447 8491773

Additional Information :

Stains second developmental MyHCI and the epitope on slow MHC is different from that of mAbs A4.840 and N2.261 [PMID 7687223]

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 52, 503-513.; Cell 68, 659-671.; J. Cell Biol. 121, 795-810.; Dev. Biol. 158, 183-199.; Invest. Ophthalmol. Vis. Sci. 41(7), 1608-1616.; Histochem. Cell. Biol. 116, 63-68.; J. Histochem. Cytochem. 50(2), 171-183.; J. Histochem. Cytochem. 51(2), 175-186.; Histochem. Cell Biol. 121, 101-107.

more info or order :

Developmental Studies Hybridoma Bank product webpage