Myosin heavy chain (human slow fibers) | Developmental Studies Hybridoma Bank A4.840 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Myosin heavy chain (human slow fibers)

catalog :

A4.840

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A4.840

reactivity :

human, mouse, chicken

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 56

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:200; loading ...; fig 3c	Wyckelsma V, Levinger I, Murphy R, Petersen A, Perry B, Hedges C, et al. Intense interval training in healthy older adults increases skeletal muscle [3H]ouabain-binding site content and elevates Na+,K+-ATPase ?2 isoform abundance in Type II fibers. Physiol Rep. 2017;5: pubmed publisher
western blot; human; 1:200; loading ...; fig 7	Perry B, Wyckelsma V, Murphy R, Steward C, Anderson M, Levinger I, et al. Dissociation between short-term unloading and resistance training effects on skeletal muscle Na+,K+-ATPase, muscle function, and fatigue in humans. J Appl Physiol (1985). 2016;121:1074-1086 pubmed publisher
immunohistochemistry; human; 1:25; loading ...; fig 3a II	Dirks M, Wall B, van de Valk B, Holloway T, Holloway G, Chabowski A, et al. One Week of Bed Rest Leads to Substantial Muscle Atrophy and Induces Whole-Body Insulin Resistance in the Absence of Skeletal Muscle Lipid Accumulation. Diabetes. 2016;65:2862-75 pubmed publisher
immunohistochemistry - frozen section; mouse; fig 5 western blot; mouse; fig 5	Stewart M, Lopez S, Nagandla H, Soibam B, Benham A, Nguyen J, et al. Mouse myofibers lacking the SMYD1 methyltransferase are susceptible to atrophy, internalization of nuclei and myofibrillar disarray. Dis Model Mech. 2016;9:347-59 pubmed publisher
immunohistochemistry - frozen section; scFv; fig 5	Valenzuela N, Fan Q, Fa ak F, Soibam B, Nagandla H, Liu Y, et al. Cardiomyocyte-specific conditional knockout of the histone chaperone HIRA in mice results in hypertrophy, sarcolemmal damage and focal replacement fibrosis. Dis Model Mech. 2016;9:335-45 pubmed publisher
western blot; human; 1:200; fig 1	Wyckelsma V, McKenna M, Levinger I, Petersen A, Lamboley C, Murphy R. Cell specific differences in the protein abundances of GAPDH and Na(+),K(+)-ATPase in skeletal muscle from aged individuals. Exp Gerontol. 2016;75:8-15 pubmed publisher
immunohistochemistry; human; 1:20; loading ...; tbl 1	Shah F, Berggren D, Holmlund T, Levring JÃ¤ghagen E, StÃ¥l P. Unique expression of cytoskeletal proteins in human soft palate muscles. J Anat. 2016;228:487-94 pubmed publisher
immunohistochemistry - frozen section; mouse; fig 1	Tallon C, Russell K, Sakhalkar S, Andrapallayal N, Farah M. Length-dependent axo-terminal degeneration at the neuromuscular synapses of type II muscle in SOD1 mice. Neuroscience. 2016;312:179-89 pubmed publisher
western blot; human; fig 1	Mohr M, Thomassen M, Girard O, Racinais S, Nybo L. Muscle variables of importance for physiological performance in competitive football. Eur J Appl Physiol. 2016;116:251-62 pubmed publisher
western blot; human; fig 2	Hostrup M, Kalsen A, Onslev J, Jessen S, Haase C, Habib S, et al. Mechanisms underlying enhancements in muscle force and power output during maximal cycle ergometer exercise induced by chronic Î²2-adrenergic stimulation in men. J Appl Physiol (1985). 2015;119:475-86 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:600; fig 9	Seaberg B, Henslee G, Wang S, Paez Colasante X, Landreth G, Rimer M. Muscle-derived extracellular signal-regulated kinases 1 and 2 are required for the maintenance of adult myofibers and their neuromuscular junctions. Mol Cell Biol. 2015;35:1238-53 pubmed publisher
immunohistochemistry; human	Janbaz A, Lindström M, Liu J, Pedrosa Domellöf F. Intermediate filaments in the human extraocular muscles. Invest Ophthalmol Vis Sci. 2014;55:5151-9 pubmed publisher
western blot; human; 1:200; fig 1a	Lamboley C, Murphy R, McKenna M, Lamb G. Sarcoplasmic reticulum Ca2+ uptake and leak properties, and SERCA isoform expression, in type I and type II fibres of human skeletal muscle. J Physiol. 2014;592:1381-95 pubmed publisher
	Morton A, Smuder A, Wiggs M, Hall S, Ahn B, Hinkley J, et al. Increased SOD2 in the diaphragm contributes to exercise-induced protection against ventilator-induced diaphragm dysfunction. Redox Biol. 2019;20:402-413 pubmed publisher
	Smeuninx B, McKendry J, Wilson D, Martin U, Breen L. Age-Related Anabolic Resistance of Myofibrillar Protein Synthesis Is Exacerbated in Obese Inactive Individuals. J Clin Endocrinol Metab. 2017;102:3535-3545 pubmed publisher
	Saha M, Mitsuhashi S, Jones M, Manko K, Reddy H, Bruels C, et al. Consequences of MEGF10 deficiency on myoblast function and Notch1 interactions. Hum Mol Genet. 2017;26:2984-3000 pubmed publisher
	Chow L, Mashek D, Wang Q, Shepherd S, Goodpaster B, Dube J. Effect of acute physiological free fatty acid elevation in the context of hyperinsulinemia on fiber type-specific IMCL accumulation. J Appl Physiol (1985). 2017;123:71-78 pubmed publisher
	Vikmoen O, Rønnestad B, Ellefsen S, Raastad T. Heavy strength training improves running and cycling performance following prolonged submaximal work in well-trained female athletes. Physiol Rep. 2017;5: pubmed publisher
	Zygmunt D, Singhal N, Kim M, Cramer M, Crowe K, Xu R, et al. Deletion of Pofut1 in Mouse Skeletal Myofibers Induces Muscle Aging-Related Phenotypes in cis and in trans. Mol Cell Biol. 2017;37: pubmed publisher
	Wyckelsma V, Levinger I, McKenna M, Formosa L, Ryan M, Petersen A, et al. Preservation of skeletal muscle mitochondrial content in older adults: relationship between mitochondria, fibre type and high-intensity exercise training. J Physiol. 2017;595:3345-3359 pubmed publisher
	Zhao K, Shen C, Lu Y, Huang Z, Li L, Rand C, et al. Muscle Yap Is a Regulator of Neuromuscular Junction Formation and Regeneration. J Neurosci. 2017;37:3465-3477 pubmed publisher
	Mohan D, Lewis A, Patel M, Curtis K, Lee J, Hopkinson N, et al. Using laser capture microdissection to study fiber specific signaling in locomotor muscle in COPD: A pilot study. Muscle Nerve. 2017;55:902-912 pubmed publisher
	Giorgetti E, Yu Z, Chua J, Shimamura R, Zhao L, Zhu F, et al. Rescue of Metabolic Alterations in AR113Q Skeletal Muscle by Peripheral Androgen Receptor Gene Silencing. Cell Rep. 2016;17:125-136 pubmed publisher
	Toral Ojeda I, Aldanondo G, Lasa Elgarresta J, Lasa Fernández H, Fernandez Torron R, Lopez de Munain A, et al. Calpain 3 deficiency affects SERCA expression and function in the skeletal muscle. Expert Rev Mol Med. 2016;18:e7 pubmed publisher
	Coughlin D, Shiels L, Nuthakki S, Shuman J. Thermal acclimation to cold alters myosin content and contractile properties of rainbow smelt, Osmerus mordax, red muscle. Comp Biochem Physiol A Mol Integr Physiol. 2016;196:46-53 pubmed publisher
	Hyatt H, Toedebusch R, Ruegsegger G, Mobley C, Fox C, McGinnis G, et al. Comparative adaptations in oxidative and glycolytic muscle fibers in a low voluntary wheel running rat model performing three levels of physical activity. Physiol Rep. 2015;3: pubmed publisher
	Guerra J, Ferrer B, Giralt M, Comes G, Carrasco J, Molinero A, et al. Muscular interleukin-6 differentially regulates skeletal muscle adaptation to high-fat diet in a sex-dependent manner. Cytokine. 2015;74:145-51 pubmed publisher
	Boman N, BurÃ©n J, Antti H, Svensson M. Gene expression and fiber type variations in repeated vastus lateralis biopsies. Muscle Nerve. 2015;52:812-7 pubmed publisher
	Coughlin D, Akhtar M. Contractile properties of the myotomal muscle of sheepshead, Archosargus probatocephalus. J Exp Zool A Ecol Genet Physiol. 2015;323:169-78 pubmed publisher
	Verhees K, Pansters N, Baarsma H, Remels A, Haegens A, de Theije C, et al. Pharmacological inhibition of GSK-3 in a guinea pig model of LPS-induced pulmonary inflammation: II. Effects on skeletal muscle atrophy. Respir Res. 2013;14:117 pubmed publisher
	Luo Q, Douglas M, BURKHOLDER T, Sokoloff A. Absence of developmental and unconventional myosin heavy chain in human suprahyoid muscles. Muscle Nerve. 2014;49:534-44 pubmed publisher
	Bentzinger C, Lin S, Romanino K, Castets P, Guridi M, Summermatter S, et al. Differential response of skeletal muscles to mTORC1 signaling during atrophy and hypertrophy. Skelet Muscle. 2013;3:6 pubmed publisher
	Osterlund C, Liu J, Thornell L, Eriksson P. Intrafusal myosin heavy chain expression of human masseter and biceps muscles at young age shows fundamental similarities but also marked differences. Histochem Cell Biol. 2013;139:895-907 pubmed publisher
	Valdez G, Tapia J, Lichtman J, Fox M, Sanes J. Shared resistance to aging and ALS in neuromuscular junctions of specific muscles. PLoS ONE. 2012;7:e34640 pubmed publisher
	Lee Y, Mikesh M, Smith I, Rimer M, Thompson W. Muscles in a mouse model of spinal muscular atrophy show profound defects in neuromuscular development even in the absence of failure in neuromuscular transmission or loss of motor neurons. Dev Biol. 2011;356:432-44 pubmed publisher
	Otto A, Macharia R, Matsakas A, Valasek P, Mankoo B, Patel K. A hypoplastic model of skeletal muscle development displaying reduced foetal myoblast cell numbers, increased oxidative myofibres and improved specific tension capacity. Dev Biol. 2010;343:51-62 pubmed publisher
	Sokoloff A, Li H, Burkholder T. Limited expression of slow tonic myosin heavy chain in human cranial muscles. Muscle Nerve. 2007;36:183-9 pubmed
	Schaart G, Hesselink R, Keizer H, Van Kranenburg G, Drost M, Hesselink M. A modified PAS stain combined with immunofluorescence for quantitative analyses of glycogen in muscle sections. Histochem Cell Biol. 2004;122:161-9 pubmed
	Salerno M, Thomas M, Forbes D, Watson T, Kambadur R, Sharma M. Molecular analysis of fiber type-specific expression of murine myostatin promoter. Am J Physiol Cell Physiol. 2004;287:C1031-40 pubmed
	Keizer H, Schaart G, Tandon N, Glatz J, Luiken J. Subcellular immunolocalisation of fatty acid translocase (FAT)/CD36 in human type-1 and type-2 skeletal muscle fibres. Histochem Cell Biol. 2004;121:101-7 pubmed
	Brazelton T, Nystrom M, Blau H. Significant differences among skeletal muscles in the incorporation of bone marrow-derived cells. Dev Biol. 2003;262:64-74 pubmed
	Liu J, Thornell L, Pedrosa Domellöf F. Muscle spindles in the deep muscles of the human neck: a morphological and immunocytochemical study. J Histochem Cytochem. 2003;51:175-86 pubmed
	Lindman R, Stål P. Abnormal palatopharyngeal muscle morphology in sleep-disordered breathing. J Neurol Sci. 2002;195:11-23 pubmed
	Liu J, Eriksson P, Thornell L, Pedrosa Domellöf F. Myosin heavy chain composition of muscle spindles in human biceps brachii. J Histochem Cytochem. 2002;50:171-83 pubmed
	Lindman R, Paulin G, Stål P. Morphological characterization of the levator veli palatini muscle in children born with cleft palates. Cleft Palate Craniofac J. 2001;38:438-48 pubmed
	Jergovic D, Stål P, Lidman D, Lindvall B, Hildebrand C. Changes in a rat facial muscle after facial nerve injury and repair. Muscle Nerve. 2001;24:1202-12 pubmed
	Stål P, Lindman R. Characterisation of human soft palate muscles with respect to fibre types, myosins and capillary supply. J Anat. 2000;197 ( Pt 2):275-90 pubmed
	Maggs A, Taylor Harris P, Peckham M, Hughes S. Evidence for differential post-translational modifications of slow myosin heavy chain during murine skeletal muscle development. J Muscle Res Cell Motil. 2000;21:101-13 pubmed
	Pedrosa Domellof F, Holmgren Y, Lucas C, Hoh J, Thornell L. Human extraocular muscles: unique pattern of myosin heavy chain expression during myotube formation. Invest Ophthalmol Vis Sci. 2000;41:1608-16 pubmed
	Shiotani A, Jones R, Flint P. Postnatal development of myosin heavy chain isoforms in rat laryngeal muscles. Ann Otol Rhinol Laryngol. 1999;108:509-15 pubmed
	Dunn S, Michel R. Coordinated expression of myosin heavy chain isoforms and metabolic enzymes within overloaded rat muscle fibers. Am J Physiol. 1997;273:C371-83 pubmed
	Tikunov B, Levine S, Mancini D. Chronic congestive heart failure elicits adaptations of endurance exercise in diaphragmatic muscle. Circulation. 1997;95:910-6 pubmed
	Michel R, Parry D, Dunn S. Regulation of myosin heavy chain expression in adult rat hindlimb muscles during short-term paralysis: comparison of denervation and tetrodotoxin-induced neural inactivation. FEBS Lett. 1996;391:39-44 pubmed
	Hughes S, Cho M, Karsch Mizrachi I, Travis M, Silberstein L, Leinwand L, et al. Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle. Dev Biol. 1993;158:183-99 pubmed
	Webster C, Silberstein L, Hays A, Blau H. Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy. Cell. 1988;52:503-13 pubmed
	Hughes S, Blau H. Muscle fiber pattern is independent of cell lineage in postnatal rodent development. Cell. 1992;68:659-71 pubmed

product information

Internal ID :

1274

Name :

A4.840

Depositor Name :

Blau, H.M.

Depositor Institution :

Baxter Lab for Stem Cell Biology, Stanford University

Depositor Website :

http://baxterlab.stanford.edu/

Date Deposited :

8/25/93

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Myosin heavy chain (human slow fibers)

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgM

Isotype for catalog (legacy) :

IgM

Positive Tested Species Reactivity :

C. elegans,Chicken,Fish,Human,Rodent

Species Tested (legacy) :

human, rodent (others?)

Initial Publication Pubmed ID :

3342447

Depositor Notes (Special Instructions) :

Stains first developmental MyHCI and the epitope on slow MHC is different from that of mAbs A4.951 and N2.261 [PMID 7687223]

Collections :

Cardiac development,Cell markers,Cytoskeleton,Human,Skeletal muscle

Search Keywords :

Blau, H. M., Myosin heavy chain (human slow fibers), Human, MIgM, Human/Rodent/Fish/C. elegans/Chicken, MYH7, CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB, AB_528384, monoclonal, Human/Cell markers/Cytoskeletal elements/Skeletal muscle/Cardiac development proteins, Immunohistochemistry/Immunofluorescence/Western Blot/ELISA/Immunoprecipitation

Antigen Molecular Weight :

220kDa

Gene :

MYH7

Alternate Gene Name(s) :

CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB

Uniprot ID :

P12883

Antibody Registry ID :

AB_528384

Immunogen :

partially purified myosin (pyrophosphate extracted) from human adult (22 year old) skeletal muscle

Clonality :

Monoclonal

Myeloma Strain :

653

Epitope Mapped :

Yes

Epitope Location or Sequence :

C-terminal excluding the first 100 aa of the N-terminal

Epitope Map PubMed ID :

7687223

Recommended Applications :

ELISA,FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

23497627 10845576 11494274 11799136 12533526 1531450 11005719 11522165 11867069 14740222 15322861 17486578 23835800 10961835 9277335 23306907

Immunofluorescence Pubmed IDs :

3342447 25703336 25982555 26592719 26945595 25676176 26597319 10961835 15189813 7687223 21658376 14512018 8706926 27680944 27681426 28498977

Western Blot Pubmed IDs :

7687223 21088205 3342447 10845576 11799136 17486578 27055500 23835800 10335716 9054750

ELISA Pubmed IDs :

7687223 3342447

FFPE Pubmed IDs :

24924099

Additional Information :

A4.480 may be used to stain skeletal muscle paraffin sections. High pH antigen retrieval is required. See PUBMED ID: 24924099 .

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 52, 503-513.; Cell 68, 659-671.; J. Cell Biol. 121, 795-810.; Dev. Biol. 158, 183-199.; Invest. Ophthalmol. Vis. Sci. 41(7), 1608-1616.; J. Anat. 197, 275-290.; Cleft Palate-Craniofacial J. 38(5), 438-448.; Muscle Nerve 24, 1202-1212.; J. Neurol. Sci. 195, 11-23.; J. Histochem. Cytochem. 50(2), 171-183.; J. Histochem. Cytochem. 51(2), 175-186.; Histochem. Cell Biol. 122, 161-169.; Histochem. Cell Biol. 121, 101-107.; Am. J. Physiol. Cell Physiol. 287, C1031-C1040.; Muscle Nerve 36, 183-189.; Dev. Biol. 343, 51-62.

more info or order :

Developmental Studies Hybridoma Bank product webpage