Myosin heavy chain (human fast fibers) | Developmental Studies Hybridoma Bank A4.74 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Myosin heavy chain (human fast fibers)

catalog :

A4.74

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A4.74

reactivity :

human, mouse, cow

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 42

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:200; loading ...; fig 3c	Wyckelsma V, Levinger I, Murphy R, Petersen A, Perry B, Hedges C, et al. Intense interval training in healthy older adults increases skeletal muscle [3H]ouabain-binding site content and elevates Na+,K+-ATPase ?2 isoform abundance in Type II fibers. Physiol Rep. 2017;5: pubmed publisher
western blot; human; 1:200; loading ...; fig 7	Perry B, Wyckelsma V, Murphy R, Steward C, Anderson M, Levinger I, et al. Dissociation between short-term unloading and resistance training effects on skeletal muscle Na+,K+-ATPase, muscle function, and fatigue in humans. J Appl Physiol (1985). 2016;121:1074-1086 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:50; fig 2	Barone R, Macaluso F, Sangiorgi C, Campanella C, Marino Gammazza A, Moresi V, et al. Skeletal muscle Heat shock protein 60 increases after endurance training and induces peroxisome proliferator-activated receptor gamma coactivator 1 Î±1 expression. Sci Rep. 2016;6:19781 pubmed publisher
western blot; human; 1:200; fig 1	Wyckelsma V, McKenna M, Levinger I, Petersen A, Lamboley C, Murphy R. Cell specific differences in the protein abundances of GAPDH and Na(+),K(+)-ATPase in skeletal muscle from aged individuals. Exp Gerontol. 2016;75:8-15 pubmed publisher
western blot; human; fig 1	Mohr M, Thomassen M, Girard O, Racinais S, Nybo L. Muscle variables of importance for physiological performance in competitive football. Eur J Appl Physiol. 2016;116:251-62 pubmed publisher
western blot; human; fig 2	Hostrup M, Kalsen A, Onslev J, Jessen S, Haase C, Habib S, et al. Mechanisms underlying enhancements in muscle force and power output during maximal cycle ergometer exercise induced by chronic Î²2-adrenergic stimulation in men. J Appl Physiol (1985). 2015;119:475-86 pubmed publisher
immunohistochemistry; human	Janbaz A, Lindström M, Liu J, Pedrosa Domellöf F. Intermediate filaments in the human extraocular muscles. Invest Ophthalmol Vis Sci. 2014;55:5151-9 pubmed publisher
western blot; human; 1:200; fig 1a	Lamboley C, Murphy R, McKenna M, Lamb G. Sarcoplasmic reticulum Ca2+ uptake and leak properties, and SERCA isoform expression, in type I and type II fibres of human skeletal muscle. J Physiol. 2014;592:1381-95 pubmed publisher
immunohistochemistry; human; 1:100	Hauerslev S, Sveen M, Vissing J, Krag T. Protein turnover and cellular stress in mildly and severely affected muscles from patients with limb girdle muscular dystrophy type 2I. PLoS ONE. 2013;8:e66929 pubmed publisher
	Choi Y, Chen P, Shin S, Zhang J, Hwang S, Lee K. Mild heat stress enhances differentiation and proliferation of Japanese quail myoblasts and enhances slow muscle fiber characteristics. Poult Sci. 2016;95:1912-7 pubmed publisher
	Chalmers H, Caswell J, Perkins J, Goodwin D, Viel L, Ducharme N, et al. Ultrasonography detects early laryngeal muscle atrophy in an equine neurectomy model. Muscle Nerve. 2016;53:583-92 pubmed publisher
	Biondi O, Branchu J, Ben Salah A, Houdebine L, Bertin L, Chali F, et al. IGF-1R Reduction Triggers Neuroprotective Signaling Pathways in Spinal Muscular Atrophy Mice. J Neurosci. 2015;35:12063-79 pubmed publisher
	Radovanovic D, Peikert K, LindstrÃ¶m M, DomellÃ¶f F. Sympathetic innervation of human muscle spindles. J Anat. 2015;226:542-8 pubmed publisher
	Guerra J, Ferrer B, Giralt M, Comes G, Carrasco J, Molinero A, et al. Muscular interleukin-6 differentially regulates skeletal muscle adaptation to high-fat diet in a sex-dependent manner. Cytokine. 2015;74:145-51 pubmed publisher
	Boman N, BurÃ©n J, Antti H, Svensson M. Gene expression and fiber type variations in repeated vastus lateralis biopsies. Muscle Nerve. 2015;52:812-7 pubmed publisher
	Peikert K, May C. Muscle spindles in the human bulbospongiosus and ischiocavernosus muscles. Muscle Nerve. 2015;52:55-62 pubmed publisher
	Bentzinger C, Lin S, Romanino K, Castets P, Guridi M, Summermatter S, et al. Differential response of skeletal muscles to mTORC1 signaling during atrophy and hypertrophy. Skelet Muscle. 2013;3:6 pubmed publisher
	Osterlund C, Liu J, Thornell L, Eriksson P. Intrafusal myosin heavy chain expression of human masseter and biceps muscles at young age shows fundamental similarities but also marked differences. Histochem Cell Biol. 2013;139:895-907 pubmed publisher
	Graziotti G, Chamizo V, Ríos C, Acevedo L, Rodríguez Menéndez J, Victorica C, et al. Adaptive functional specialisation of architectural design and fibre type characteristics in agonist shoulder flexor muscles of the llama, Lama glama. J Anat. 2012;221:151-63 pubmed publisher
	Sieck D, Zhan W, Fang Y, Ermilov L, Sieck G, Mantilla C. Structure-activity relationships in rodent diaphragm muscle fibers vs. neuromuscular junctions. Respir Physiol Neurobiol. 2012;180:88-96 pubmed publisher
	Duris M, Picard B, Geay Y. Specificity of different anti-myosin heavy chain antibodies in bovine muscle. Meat Sci. 2000;55:67-78 pubmed
	Lee Y, Mikesh M, Smith I, Rimer M, Thompson W. Muscles in a mouse model of spinal muscular atrophy show profound defects in neuromuscular development even in the absence of failure in neuromuscular transmission or loss of motor neurons. Dev Biol. 2011;356:432-44 pubmed publisher
	Scime A, Soleimani V, Bentzinger C, Gillespie M, Le Grand F, Grenier G, et al. Oxidative status of muscle is determined by p107 regulation of PGC-1alpha. J Cell Biol. 2010;190:651-62 pubmed publisher
	Zhou Y, Liu D, Kaminski H. Myosin heavy chain expression in mouse extraocular muscle: more complex than expected. Invest Ophthalmol Vis Sci. 2010;51:6355-63 pubmed publisher
	Otto A, Macharia R, Matsakas A, Valasek P, Mankoo B, Patel K. A hypoplastic model of skeletal muscle development displaying reduced foetal myoblast cell numbers, increased oxidative myofibres and improved specific tension capacity. Dev Biol. 2010;343:51-62 pubmed publisher
	Graziotti G, Palencia P, Delhon G, Rivero J. Neuromuscular partitioning, architectural design, and myosin fiber types of the M. vastus lateralis of the llama (Lama glama). J Morphol. 2004;262:667-81 pubmed
	Young H, Black A. Adult stem cells. Anat Rec A Discov Mol Cell Evol Biol. 2004;276:75-102 pubmed
	Young H. Existence of reserve quiescent stem cells in adults, from amphibians to humans. Curr Top Microbiol Immunol. 2004;280:71-109 pubmed
	Brazelton T, Nystrom M, Blau H. Significant differences among skeletal muscles in the incorporation of bone marrow-derived cells. Dev Biol. 2003;262:64-74 pubmed
	Liu J, Thornell L, Pedrosa Domellöf F. Muscle spindles in the deep muscles of the human neck: a morphological and immunocytochemical study. J Histochem Cytochem. 2003;51:175-86 pubmed
	Liu J, Eriksson P, Thornell L, Pedrosa Domellöf F. Myosin heavy chain composition of muscle spindles in human biceps brachii. J Histochem Cytochem. 2002;50:171-83 pubmed
	Young H, Steele T, Bray R, Hudson J, Floyd J, Hawkins K, et al. Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors. Anat Rec. 2001;264:51-62 pubmed
	Jergovic D, Stål P, Lidman D, Lindvall B, Hildebrand C. Changes in a rat facial muscle after facial nerve injury and repair. Muscle Nerve. 2001;24:1202-12 pubmed
	Maggs A, Taylor Harris P, Peckham M, Hughes S. Evidence for differential post-translational modifications of slow myosin heavy chain during murine skeletal muscle development. J Muscle Res Cell Motil. 2000;21:101-13 pubmed
	Pedrosa Domellof F, Holmgren Y, Lucas C, Hoh J, Thornell L. Human extraocular muscles: unique pattern of myosin heavy chain expression during myotube formation. Invest Ophthalmol Vis Sci. 2000;41:1608-16 pubmed
	Shiotani A, Westra W, Flint P. Myosin heavy chain composition in human laryngeal muscles. Laryngoscope. 1999;109:1521-4 pubmed
	Shiotani A, Jones R, Flint P. Postnatal development of myosin heavy chain isoforms in rat laryngeal muscles. Ann Otol Rhinol Laryngol. 1999;108:509-15 pubmed
	Tikunov B, Levine S, Mancini D. Chronic congestive heart failure elicits adaptations of endurance exercise in diaphragmatic muscle. Circulation. 1997;95:910-6 pubmed
	Tikunov B, Mancini D, Levine S. Changes in myofibrillar protein composition of human diaphragm elicited by congestive heart failure. J Mol Cell Cardiol. 1996;28:2537-41 pubmed
	Hughes S, Cho M, Karsch Mizrachi I, Travis M, Silberstein L, Leinwand L, et al. Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle. Dev Biol. 1993;158:183-99 pubmed
	Webster C, Silberstein L, Hays A, Blau H. Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy. Cell. 1988;52:503-13 pubmed
	Hughes S, Blau H. Muscle fiber pattern is independent of cell lineage in postnatal rodent development. Cell. 1992;68:659-71 pubmed

product information

Internal ID :

1268

Name :

A4.74

Depositor Name :

Blau, H.M.

Depositor Institution :

Baxter Lab for Stem Cell Biology, Stanford University

Depositor Website :

http://baxterlab.stanford.edu/

Date Deposited :

8/25/93

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Myosin heavy chain (human fast fibers)

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Bovine,C. elegans,Horse,Human,Llama,Porcine,Quail,Rodent

Species Tested (legacy) :

human, rodent (others?)

Initial Publication Pubmed ID :

3342447

Depositor Notes (Special Instructions) :

Fusion: 1983. Recognizes all fast fibers in human. In rat, high affinity for IIA fibers, low affinity for IIX/D fibers, and doesn't stain IIB.

Collections :

Cardiac development,Cell markers,Cytoskeleton,Human,Skeletal muscle

Search Keywords :

Blau, H. M., Myosin heavy chain (human fast fibers), Human, MIgG1, Human/Rodent/ Horse/Llama/Quail/Bovine/Porcine/C. elegans, MYH2, BM3; MYH2A; MYPOP; MYHSA2; MYHas8; MyHC-2A; MyHC-IIa, AB_528383, monoclonal, Human/Cell markers/Cytoskeletal elements/Skeletal muscle/Cardiac development proteins, Immunohistochemistry/Immunofluorescence/Western Blot/ELISA/Immunoprecipitation

Antigen Molecular Weight :

220kDa

Gene :

MYH2

Alternate Gene Name(s) :

BM3; MYH2A; MYPOP; MYHSA2; MYHas8; MyHC-2A; MyHC-IIa

Uniprot ID :

Q9UKX2

Antibody Registry ID :

AB_528383

Immunogen :

partially purified myosin (pyrophosphate extracted) from human adult (22 year old) skeletal muscle

Clonality :

Monoclonal

Myeloma Strain :

653

Epitope Mapped :

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

23497627 20417199 15376272 10845576 11505371 11494274 11799136 12533526 14594208 14699636 22625659 1531450 25363905 10961835 23306907

Immunofluorescence Pubmed IDs :

3342447 25994126 25703336 25982555 26227954 22063925 22060906 7687223 20610840 21658376 26311784 14512018

Western Blot Pubmed IDs :

7687223 3342447 21088205 10845576 11799136 27038421 22060906 10335716 9054750 10499066 9004169

ELISA Pubmed IDs :

7687223 3342447

Additional Information :

stains MyHCIIa [PMID 7687223]. This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 52, 503-513.; Cell 68, 659-671.; J. Cell Biol. 121, 795-810.; Dev. Biol. 158, 183-199.; Invest. Ophthalmol. Vis. Sci. 41(7), 1608-1616.; Anat. Rec. 264, 51-62.; Muscle Nerve 24, 1202-1212.; J. Histochem. Cytochem. 50(2), 171-183.; J. Histochem. Cytochem. 51(2), 175-186.; Curr. Top. Microbiol. Immunol. 280, 71-109.; Anat. Rec. Part A 276A, 75-102.; J. Morphol. 262, 667-681.; InVet 6(1), 21-27.; Dev. Biol. 343, 51-62.; Asian-Aust. J. Anim. Sci. 24(1), 125-129.; J. Anat. doi:10.1111/j.1469-7580.2012.01520.x

more info or order :

Developmental Studies Hybridoma Bank product webpage