Myosin heavy chain (human nascent secondary and all fast fibers) | Developmental Studies Hybridoma Bank A4.1519 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Myosin heavy chain (human nascent secondary and all fast fibers)

catalog :

A4.1519

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A4.1519

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 8

Reference
Gillies A, Smith L, Lieber R, Varghese S. Method for decellularizing skeletal muscle without detergents or proteolytic enzymes. Tissue Eng Part C Methods. 2011;17:383-9 pubmed publisher
Brazelton T, Nystrom M, Blau H. Significant differences among skeletal muscles in the incorporation of bone marrow-derived cells. Dev Biol. 2003;262:64-74 pubmed
Shiotani A, Westra W, Flint P. Myosin heavy chain composition in human laryngeal muscles. Laryngoscope. 1999;109:1521-4 pubmed
Shiotani A, Jones R, Flint P. Postnatal development of myosin heavy chain isoforms in rat laryngeal muscles. Ann Otol Rhinol Laryngol. 1999;108:509-15 pubmed
Tikunov B, Levine S, Mancini D. Chronic congestive heart failure elicits adaptations of endurance exercise in diaphragmatic muscle. Circulation. 1997;95:910-6 pubmed
Tikunov B, Mancini D, Levine S. Changes in myofibrillar protein composition of human diaphragm elicited by congestive heart failure. J Mol Cell Cardiol. 1996;28:2537-41 pubmed
Webster C, Silberstein L, Hays A, Blau H. Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy. Cell. 1988;52:503-13 pubmed
Hughes S, Blau H. Muscle fiber pattern is independent of cell lineage in postnatal rodent development. Cell. 1992;68:659-71 pubmed

product information

Internal ID :

1262

Name :

A4.1519

Depositor Name :

Blau, H.M.

Depositor Institution :

Baxter Lab for Stem Cell Biology, Stanford University

Depositor Website :

http://baxterlab.stanford.edu/

Date Deposited :

8/25/93

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Myosin heavy chain (human nascent secondary and all fast fibers)

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgM

Isotype for catalog (legacy) :

IgM

Positive Tested Species Reactivity :

Human,Porcine,Rodent

Species Tested (legacy) :

human, rodent (others?)

Initial Publication Pubmed ID :

3342447

Depositor Notes (Special Instructions) :

Fusion: 1983. Recognizes nascent secondary and all fast adult fibers in human. In rat, high affinity for IIA fibers, low affinity for IIB fibers, and doesn't stain IIX/D.

Collections :

Cardiac development,Cell markers,Cytoskeleton,Human,Skeletal muscle

Search Keywords :

Blau, H. M., Myosin heavy chain (human nascent secondary and all fast fibers), Human, MIgM, Human/Rodent/Porcine, MYH2, BM3; MYH2A; MYPOP; MYHSA2; MYHas8; MyHC-2A; MyHC-IIa, AB_528357, monoclonal, Human/Cell markers/Cytoskeletal elements/Skeletal muscle/Cardiac development proteins, Immunohistochemistry/Immunofluorescence/Western Blot/ELISA/Immunoprecipitation

Antigen Molecular Weight :

220kDa

Gene :

MYH2

Alternate Gene Name(s) :

BM3; MYH2A; MYPOP; MYHSA2; MYHas8; MyHC-2A; MyHC-IIa

Uniprot ID :

Q9UKX2

Antibody Registry ID :

AB_528357

Immunogen :

partially purified myosin (pyrophosphate extracted) from adult (22 year old) skeletal muscle

Clonality :

Monoclonal

Myeloma Strain :

653

Epitope Mapped :

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

1531450

Immunofluorescence Pubmed IDs :

14512018

Western Blot Pubmed IDs :

10335716 3342447 9054750 10499066 9004169 20973753

ELISA Pubmed IDs :

3342447

Additional Information :

This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 52, 503-513.; Cell 68, 659-671.; J. Cell Biol. 121, 795-810.; Dev. Biol. 158, 183-199.; Asian-Aust. J. Anim. Sci. 24(1), 125-129.

more info or order :

Developmental Studies Hybridoma Bank product webpage