product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Myosin all isoforms
catalog :
A4.1025
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A4.1025
reactivity :
human, mouse, rat, zebrafish
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 65
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; zebrafish ; 1:1000; loading ...; fig 8d
Berberoglu M, Gallagher T, Morrow Z, Talbot J, Hromowyk K, Tenente I, et al. Satellite-like cells contribute to pax7-dependent skeletal muscle repair in adult zebrafish. Dev Biol. 2017;424:162-180 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:10; fig 6
Antony N, McDougall A, Mantamadiotis T, Cole T, Bird A. Creb1 regulates late stage mammalian lung development via respiratory epithelial and mesenchymal-independent mechanisms. Sci Rep. 2016;6:25569 pubmed publisher
  • western blot; human; fig 3
Toral Ojeda I, Aldanondo G, Lasa Elgarresta J, Lasa Fernández H, Fernandez Torron R, Lopez de Munain A, et al. Calpain 3 deficiency affects SERCA expression and function in the skeletal muscle. Expert Rev Mol Med. 2016;18:e7 pubmed publisher
  • immunohistochemistry; zebrafish ; 1:10; fig 4
Ruparelia A, Oorschot V, Ramm G, Bryson Richardson R. FLNC myofibrillar myopathy results from impaired autophagy and protein insufficiency. Hum Mol Genet. 2016;25:2131-2142 pubmed
  • immunocytochemistry; human; 1:100; fig 7
Neems D, Garza Gongora A, Smith E, Kosak S. Topologically associated domains enriched for lineage-specific genes reveal expression-dependent nuclear topologies during myogenesis. Proc Natl Acad Sci U S A. 2016;113:E1691-700 pubmed publisher
  • immunohistochemistry; mouse; 1:2; fig 1
Kraft Sheleg O, Zaffryar Eilot S, Genin O, Yaseen W, Soueid Baumgarten S, Kessler O, et al. Localized LoxL3-Dependent Fibronectin Oxidation Regulates Myofiber Stretch and Integrin-Mediated Adhesion. Dev Cell. 2016;36:550-61 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:50; fig 6
Barthold J, Pugarelli J, Macdonald M, Ren J, Adetunji M, Polson S, et al. Polygenic inheritance of cryptorchidism susceptibility in the LE/orl rat. Mol Hum Reprod. 2016;22:18-34 pubmed publisher
  • immunohistochemistry; zebrafish ; 1:10
Ruparelia A, Oorschot V, Vaz R, Ramm G, Bryson Richardson R. Zebrafish models of BAG3 myofibrillar myopathy suggest a toxic gain of function leading to BAG3 insufficiency. Acta Neuropathol. 2014;128:821-33 pubmed publisher
  • immunohistochemistry; zebrafish ; 1:250; fig 2
Subramanian A, Schilling T. Thrombospondin-4 controls matrix assembly during development and repair of myotendinous junctions. elife. 2014;3: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:4
Nicklas S, Otto A, Wu X, Miller P, Stelzer S, Wen Y, et al. TRIM32 regulates skeletal muscle stem cell differentiation and is necessary for normal adult muscle regeneration. PLoS ONE. 2012;7:e30445 pubmed publisher
Morgan J, Robbins A, Mateson A, Sawamoto K, Tomatsu S, Gray D, et al. Regional Variation in Androgen Receptor Expression and Biomechanical Properties May Contribute to Cryptorchidism Susceptibility in the LE/orl Rat. Front Endocrinol (Lausanne). 2018;9:738 pubmed publisher
Fenix A, Neininger A, Taneja N, Hyde K, Visetsouk M, Garde R, et al. Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes. elife. 2018;7: pubmed publisher
Subramanian A, Kanzaki L, Galloway J, Schilling T. Mechanical force regulates tendon extracellular matrix organization and tenocyte morphogenesis through TGFbeta signaling. elife. 2018;7: pubmed publisher
Ma R, Jacobs C, Sharma P, Kocha K, Huang P. Stereotypic generation of axial tenocytes from bipartite sclerotome domains in zebrafish. PLoS Genet. 2018;14:e1007775 pubmed publisher
Ganassi M, Badodi S, Ortuste Quiroga H, Zammit P, Hinits Y, Hughes S. Myogenin promotes myocyte fusion to balance fibre number and size. Nat Commun. 2018;9:4232 pubmed publisher
Mueller A, Cichewicz M, Dey B, Layer R, Reon B, Gagan J, et al. MUNC, a long noncoding RNA that facilitates the function of MyoD in skeletal myogenesis. Mol Cell Biol. 2015;35:498-513 pubmed publisher
Lee K, Skromne I. Retinoic acid regulates size, pattern and alignment of tissues at the head-trunk transition. Development. 2014;141:4375-84 pubmed publisher
McAleer C, Smith A, Najjar S, Pirozzi K, Long C, Hickman J. Mechanistic investigation of adult myotube response to exercise and drug treatment in vitro using a multiplexed functional assay system. J Appl Physiol (1985). 2014;117:1398-405 pubmed publisher
Chen Y, Chen C, Yeh S, Lin Y, Wu Y, Hsieh W, et al. Urethral dysfunction in female mice with estrogen receptor β deficiency. PLoS ONE. 2014;9:e109058 pubmed publisher
Barthold J, Robbins A, Wang Y, Pugarelli J, Mateson A, Anand Ivell R, et al. Cryptorchidism in the orl rat is associated with muscle patterning defects in the fetal gubernaculum and altered hormonal signaling. Biol Reprod. 2014;91:41 pubmed publisher
Iannotti F, Silvestri C, Mazzarella E, Martella A, Calvigioni D, Piscitelli F, et al. The endocannabinoid 2-AG controls skeletal muscle cell differentiation via CB1 receptor-dependent inhibition of Kv7 channels. Proc Natl Acad Sci U S A. 2014;111:E2472-81 pubmed publisher
Ganassi M, Badodi S, Polacchini A, Baruffaldi F, Battini R, Hughes S, et al. Distinct functions of alternatively spliced isoforms encoded by zebrafish mef2ca and mef2cb. Biochim Biophys Acta. 2014;1839:559-70 pubmed publisher
Chen J, Galloway J. The development of zebrafish tendon and ligament progenitors. Development. 2014;141:2035-45 pubmed publisher
Liang S, Li H, Wang Y, Wu S, Cai Y, Cui H, et al. Pulmonary endoderm, second heart field and the morphogenesis of distal outflow tract in mouse embryonic heart. Dev Growth Differ. 2014;56:276-92 pubmed publisher
O Brien J, Hernandez Lagunas L, Artinger K, Ford H. MicroRNA-30a regulates zebrafish myogenesis through targeting the transcription factor Six1. J Cell Sci. 2014;127:2291-301 pubmed publisher
Bareja A, Holt J, Luo G, Chang C, Lin J, Hinken A, et al. Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis. PLoS ONE. 2014;9:e90398 pubmed publisher
Madison R, McGee C, Rawson R, Robinson G. Extracellular vesicles from a muscle cell line (C2C12) enhance cell survival and neurite outgrowth of a motor neuron cell line (NSC-34). J Extracell Vesicles. 2014;3: pubmed publisher
Clark C, Austen O, Poparic I, Guthrie S. ?2-Chimaerin regulates a key axon guidance transition during development of the oculomotor projection. J Neurosci. 2013;33:16540-51 pubmed publisher
de Carlos F, Cobo J, Macias E, Feito J, Cobo T, Calavia M, et al. The sensory innervation of the human pharynx: searching for mechanoreceptors. Anat Rec (Hoboken). 2013;296:1735-46 pubmed publisher
Wolny M, Colegrave M, Colman L, White E, Knight P, Peckham M. Cardiomyopathy mutations in the tail of ?-cardiac myosin modify the coiled-coil structure and affect integration into thick filaments in muscle sarcomeres in adult cardiomyocytes. J Biol Chem. 2013;288:31952-62 pubmed publisher
Pirozzi K, Long C, McAleer C, Smith A, Hickman J. Correlation of embryonic skeletal muscle myotube physical characteristics with contractile force generation on an atomic force microscope-based bio-microelectromechanical systems device. Appl Phys Lett. 2013;103:83108 pubmed
Atlasi Y, Noori R, Gaspar C, Franken P, Sacchetti A, Rafati H, et al. Wnt signaling regulates the lineage differentiation potential of mouse embryonic stem cells through Tcf3 down-regulation. PLoS Genet. 2013;9:e1003424 pubmed publisher
Berger J, Currie P. 503unc, a small and muscle-specific zebrafish promoter. Genesis. 2013;51:443-7 pubmed publisher
Ruparelia A, Zhao M, Currie P, Bryson Richardson R. Characterization and investigation of zebrafish models of filamin-related myofibrillar myopathy. Hum Mol Genet. 2012;21:4073-83 pubmed publisher
Banfi S, Monti L, Acquati F, Tettamanti G, de Eguileor M, Grimaldi A. Muscle development and differentiation in the urodele Ambystoma mexicanum. Dev Growth Differ. 2012;54:489-502 pubmed publisher
Park K, Lim J, Sohn S, Oh S. Myosin heavy chain isoform expression in human extraocular muscles: longitudinal variation and patterns of expression in global and orbital layers. Muscle Nerve. 2012;45:713-20 pubmed publisher
Bai X, Wu X, Wang X, Liu X, Song Y, Gao F, et al. Inhibition of mammalian muscle differentiation by excretory secretory products of muscle larvae of Trichinella spiralis in vitro. Parasitol Res. 2012;110:2481-90 pubmed publisher
Collins Hooper H, Luke G, Cranfield M, Otto W, Ray S, Patel K. Efficient myogenic reprogramming of adult white fat stem cells and bone marrow stem cells by freshly isolated skeletal muscle fibers. Transl Res. 2011;158:334-43 pubmed publisher
Cole N, Hall T, Don E, Berger S, Boisvert C, Neyt C, et al. Development and evolution of the muscles of the pelvic fin. PLoS Biol. 2011;9:e1001168 pubmed publisher
Valasek P, Theis S, DeLaurier A, Hinits Y, Luke G, Otto A, et al. Cellular and molecular investigations into the development of the pectoral girdle. Dev Biol. 2011;357:108-16 pubmed publisher
Bilousova G, Jun D, King K, De Langhe S, Chick W, Torchia E, et al. Osteoblasts derived from induced pluripotent stem cells form calcified structures in scaffolds both in vitro and in vivo. Stem Cells. 2011;29:206-16 pubmed publisher
Madhala Levy D, Williams V, Hughes S, Reshef R, Halevy O. Cooperation between Shh and IGF-I in promoting myogenic proliferation and differentiation via the MAPK/ERK and PI3K/Akt pathways requires Smo activity. J Cell Physiol. 2012;227:1455-64 pubmed publisher
Ayala R, Zhang C, Yang D, Hwang Y, Aung A, Shroff S, et al. Engineering the cell-material interface for controlling stem cell adhesion, migration, and differentiation. Biomaterials. 2011;32:3700-11 pubmed publisher
Schabort E, van der Merwe M, Niesler C. TGF-? isoforms inhibit IGF-1-induced migration and regulate terminal differentiation in a cell-specific manner. J Muscle Res Cell Motil. 2011;31:359-67 pubmed publisher
Ratti J, Rostkova E, Gautel M, Pfuhl M. Structure and interactions of myosin-binding protein C domain C0: cardiac-specific regulation of myosin at its neck?. J Biol Chem. 2011;286:12650-8 pubmed publisher
Bilousova G, Chen J, Roop D. Differentiation of mouse induced pluripotent stem cells into a multipotent keratinocyte lineage. J Invest Dermatol. 2011;131:857-64 pubmed publisher
Geach T, Zimmerman L. Paralysis and delayed Z-disc formation in the Xenopus tropicalis unc45b mutant dicky ticker. BMC Dev Biol. 2010;10:75 pubmed publisher
Etard C, Roostalu U, Strahle U. Lack of Apobec2-related proteins causes a dystrophic muscle phenotype in zebrafish embryos. J Cell Biol. 2010;189:527-39 pubmed publisher
Pisani D, Dechesne C, Sacconi S, Delplace S, Belmonte N, Cochet O, et al. Isolation of a highly myogenic CD34-negative subset of human skeletal muscle cells free of adipogenic potential. Stem Cells. 2010;28:753-64 pubmed publisher
Sato Y, Probst H, Tatsumi R, Ikeuchi Y, Neuberger M, Rada C. Deficiency in APOBEC2 leads to a shift in muscle fiber type, diminished body mass, and myopathy. J Biol Chem. 2010;285:7111-8 pubmed publisher
Tee J, van Rooijen C, Boonen R, Zivkovic D. Regulation of slow and fast muscle myofibrillogenesis by Wnt/beta-catenin and myostatin signaling. PLoS ONE. 2009;4:e5880 pubmed publisher
Mishima Y, Abreu Goodger C, Staton A, Stahlhut C, Shou C, Cheng C, et al. Zebrafish miR-1 and miR-133 shape muscle gene expression and regulate sarcomeric actin organization. Genes Dev. 2009;23:619-32 pubmed publisher
Schnapp E, Pistocchi A, Karampetsou E, Foglia E, Lamia C, Cotelli F, et al. Induced early expression of mrf4 but not myog rescues myogenesis in the myod/myf5 double-morphant zebrafish embryo. J Cell Sci. 2009;122:481-8 pubmed publisher
Bessarab D, Chong S, Srinivas B, Korzh V. Six1a is required for the onset of fast muscle differentiation in zebrafish. Dev Biol. 2008;323:216-28 pubmed publisher
Du S, Li H, Bian Y, Zhong Y. Heat-shock protein 90alpha1 is required for organized myofibril assembly in skeletal muscles of zebrafish embryos. Proc Natl Acad Sci U S A. 2008;105:554-9 pubmed publisher
Yokoyama T, Takano K, Yoshida A, Katada F, Sun P, Takenawa T, et al. DA-Raf1, a competent intrinsic dominant-negative antagonist of the Ras-ERK pathway, is required for myogenic differentiation. J Cell Biol. 2007;177:781-93 pubmed
Maggs A, Taylor Harris P, Peckham M, Hughes S. Evidence for differential post-translational modifications of slow myosin heavy chain during murine skeletal muscle development. J Muscle Res Cell Motil. 2000;21:101-13 pubmed
Holley S, Geisler R, Nusslein Volhard C. Control of her1 expression during zebrafish somitogenesis by a delta-dependent oscillator and an independent wave-front activity. Genes Dev. 2000;14:1678-90 pubmed
Hook P, Li X, Sleep J, Hughes S, Larsson L. In vitro motility speed of slow myosin extracted from single soleus fibres from young and old rats. J Physiol. 1999;520 Pt 2:463-71 pubmed
Shiotani A, Westra W, Flint P. Myosin heavy chain composition in human laryngeal muscles. Laryngoscope. 1999;109:1521-4 pubmed
Shiotani A, Flint P. Myosin heavy chain composition in rat laryngeal muscles after denervation. Laryngoscope. 1998;108:1225-9 pubmed
Cho M, Webster S, Blau H. Evidence for myoblast-extrinsic regulation of slow myosin heavy chain expression during muscle fiber formation in embryonic development. J Cell Biol. 1993;121:795-810 pubmed
Hughes S, Cho M, Karsch Mizrachi I, Travis M, Silberstein L, Leinwand L, et al. Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle. Dev Biol. 1993;158:183-99 pubmed
Cho M, Hughes S, Karsch Mizrachi I, Travis M, Leinwand L, Blau H. Fast myosin heavy chains expressed in secondary mammalian muscle fibers at the time of their inception. J Cell Sci. 1994;107 ( Pt 9):2361-71 pubmed
Webster C, Silberstein L, Hays A, Blau H. Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy. Cell. 1988;52:503-13 pubmed
product information
Internal ID :
1256
Name :
A4.1025
Depositor Name :
Blau, H.M.
Depositor Institution :
Baxter Lab for Stem Cell Biology, Stanford University
Depositor Website :
http://baxterlab.stanford.edu/
Date Deposited :
8/25/93
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Myosin all isoforms
Antigen Species :
Human
Host Species :
mouse
Isotype :
MIgG2a
Isotype for catalog (legacy) :
IgG2a
Positive Tested Species Reactivity :
Amphibian,Fish,Human,Rodent,Zebrafish
Species Tested (legacy) :
human, rodent, zebrafish (others?)
Initial Publication Pubmed ID :
7687223 3342447
Collections :
Cardiac development,Cytoskeleton,Human,Skeletal muscle
Search Keywords :
Blau, Helen M., Myosin [all isoforms] , Human, MIgG2a, Human/Rodent/Fish/Amphibian, MYH1, MYHa; HEL71; MYHSA1; MyHC-2x; MyHC-2X/D, AB_528356, monoclonal
Antigen Molecular Weight :
220 kDa
Gene :
MYH1
Alternate Gene Name(s) :
MYHa, HEL71, MYHSA1, MyHC-2x, MyHC-2X/D
Uniprot ID :
P12882
Antibody Registry ID :
AB_528356
Immunogen :
Myosin heavy chain (human MHC or MyHC) purified.
Clonality :
Monoclonal
Myeloma Strain :
P3 x 63Ag8 653
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
10961835 24844180 24634509 25273835 24587351 24803652 24697670 25371368 20637071 22499099 24047955 23444339 22519643 21741963 22706277 24046483 22061041 24123994 21990962 24133258 20440001 19240126 19193870 19517013 18789916 18182494 10887161
Immunofluorescence Pubmed IDs :
10523415 24844180 24966393 24634509 25273835 25301895 24803652 25403490 21297165 20637071 22200963 21732479 23444339 22519643 21618536 22706277 20135684 21150926 21396708 22061041 21990962 30388110
Western Blot Pubmed IDs :
8491773 9707248 7531198 7687223 10499066 10961835 25275480 24563732 24927567 24844180 22200963 22499099 24047955 21298471 22519643 21618536 20022958
ELISA Pubmed IDs :
7687223
DSHB Growth Medium :
Iscove's
References (legacy) :
Cell 52, 503-513.; Cell 68, 659-671.; J. Cell Biol. 121, 795-810.; Dev. Biol. 158, 183-199.; J. Muscle Res. Cell Motil. 31, 359-367.; Tiss. Eng. 20(1), doi:10.1089/ten.tec.2013.0146.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US